Q7Z3V4 (UBE3B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-protein ligase E3B
EC=6.3.2.-

Gene names

Name:

UBE3B

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1068 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates By similarity.
Pathway	Protein modification; protein ubiquitination.
Tissue specificity	Widely expressed. Ref.1
Sequence similarities	Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. Contains 1 IQ domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	Ligase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein ubiquitination involved in ubiquitin-dependent protein catabolic process Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	ubiquitin-protein ligase activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q7Z3V4-1)

Also known as: UBE3B_v1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Major isoform.

Isoform 2 (identifier: Q7Z3V4-2)

Also known as: UBE3B_v2;

The sequence of this isoform differs from the canonical sequence as follows:
     693-708: DGYEQLRQLSQHAMKG → SLFECPWPLVINAESC
     709-1068: Missing.

Isoform 3 (identifier: Q7Z3V4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     211-244: ILLTRGLARPRPCLSKGTLTAAFSLALRPVIAAQ → CCDGLFPDLVSYAPHNNPVRWSVGRSWYDWQLSR
     245-1068: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1068	1068	Ubiquitin-protein ligase E3B		PRO_0000281882
Regions
	Domain	29 – 58	30	IQ
	Domain	702 – 1068	367	HECT
Sites
	Active site	1036	1	Glycyl thioester intermediate By similarity
Amino acid modifications
	Modified residue	419	1	Phosphoserine Ref.5
Natural variations
	Alternative sequence	211 – 244	34	ILLTR…VIAAQ → CCDGLFPDLVSYAPHNNPVR WSVGRSWYDWQLSR in isoform 3.		VSP_024085
	Alternative sequence	245 – 1068	824	Missing in isoform 3.		VSP_024086
	Alternative sequence	693 – 708	16	DGYEQ…HAMKG → SLFECPWPLVINAESC in isoform 2.		VSP_024087
	Alternative sequence	709 – 1068	360	Missing in isoform 2.		VSP_024088
	Natural variant	346	1	R → Q. Ref.2 Ref.4 Corresponds to variant rs7298565 [ dbSNP | Ensembl ].		VAR_031302
Experimental info
	Sequence conflict	151	1	E → V in CAD97645. Ref.2
	Sequence conflict	907	1	I → V in CAD97645. Ref.2
	Sequence conflict	942	1	Y → H in CAD97645. Ref.2
	Sequence conflict	1018	1	F → S in CAD97645. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (UBE3B_v1) [UniParc]. Last modified May 18, 2010. Version 3. Checksum: A1740A3DDC5E1A4D	FASTA	1,068	123,098
	10 20 30 40 50 60 MFTLSQTSRA WFIDRARQAR EERLVQKERE RAAVVIQAHV RSFLCRSRLQ RDIRREIDDF 70 80 90 100 110 120 FKADDPESTK RSALCIFKIA RKLLFLFRIK EDNERFEKLC RSILSSMDAE NEPKVWYVSL 130 140 150 160 170 180 ACSKDLTLLW IQQIKNILWY CCDFLKQLKP EILQDSRLIT LYLTMLVTFT DTSTWKILRG 190 200 210 220 230 240 KGESLRPAMN HICANIMGHL NQHGFYSVLQ ILLTRGLARP RPCLSKGTLT AAFSLALRPV 250 260 270 280 290 300 IAAQFSDNLI RPFLIHIMSV PALVTHLSTV TPERLTVLES HDMLRKFIIF LRDQDRCRDV 310 320 330 340 350 360 CESLEGCHTL CLMGNLLHLG SLSPRVLEEE TDGFVSLLTQ TLCYCRKYVS QKKSNLTHWH 370 380 390 400 410 420 PVLGWFSQSV DYGLNESMHL ITKQLQFLWG VPLIRIFFCD ILSKKLLESQ EPAHAQPASP 430 440 450 460 470 480 QNVLPVKSLL KRAFQKSASV RNILRPVGGK RVDSAEVQKV CNICVLYQTS LTTLTQIRLQ 490 500 510 520 530 540 ILTGLTYLDD LLPKLWAFIC ELGPHGGLKL FLECLNNDTE ESKQLLAMLM LFCDCSRHLI 550 560 570 580 590 600 TILDDIEVYE EQISFKLEEL VTISSFLNSF VFKMIWDGIV ENAKGETLEL FQSVHGWLMV 610 620 630 640 650 660 LYERDCRRRF TPEDHWLRKD LKPSVLFQEL DRDRKRAQLI LQYIPHVIPH KNRVLLFRTM 670 680 690 700 710 720 VTKEKEKLGL VETSSASPHV THITIRRSRM LEDGYEQLRQ LSQHAMKGVI RVKFVNDLGV 730 740 750 760 770 780 DEAGIDQDGV FKEFLEEIIK RVFDPALNLF KTTSGDERLY PSPTSYIHEN YLQLFEFVGK 790 800 810 820 830 840 MLGKAVYEGI VVDVPFASFF LSQLLGHHHS VFYSSVDELP SLDSEFYKNL TSIKRYDGDI 850 860 870 880 890 900 TDLGLTLSYD EDVMGQLVCH ELIPGGKTIP VTNENKISYI HLMAHFRMHT QIKNQTAALI 910 920 930 940 950 960 SGFRSIIKPE WIRMFSTPEL QRLISGDNAE IDLEDLKKHT VYYGGFHGSH RVIIWLWDIL 970 980 990 1000 1010 1020 ASDFTPDERA MFLKFVTSCS RPPLLGFAYL KPPFSIRCVE VSDDQDTGDT LGSVLRGFFT 1030 1040 1050 1060 IRKREPGGRL PTSSTCFNLL KLPNYSKKSV LREKLRYAIS MNTGFELS
	Isoform 2 (UBE3B_v2) [UniParc]. Checksum: A4BF5D68DEFA6DD2	FASTA	708	82,097
	10 20 30 40 50 60 MFTLSQTSRA WFIDRARQAR EERLVQKERE RAAVVIQAHV RSFLCRSRLQ RDIRREIDDF 70 80 90 100 110 120 FKADDPESTK RSALCIFKIA RKLLFLFRIK EDNERFEKLC RSILSSMDAE NEPKVWYVSL 130 140 150 160 170 180 ACSKDLTLLW IQQIKNILWY CCDFLKQLKP EILQDSRLIT LYLTMLVTFT DTSTWKILRG 190 200 210 220 230 240 KGESLRPAMN HICANIMGHL NQHGFYSVLQ ILLTRGLARP RPCLSKGTLT AAFSLALRPV 250 260 270 280 290 300 IAAQFSDNLI RPFLIHIMSV PALVTHLSTV TPERLTVLES HDMLRKFIIF LRDQDRCRDV 310 320 330 340 350 360 CESLEGCHTL CLMGNLLHLG SLSPRVLEEE TDGFVSLLTQ TLCYCRKYVS QKKSNLTHWH 370 380 390 400 410 420 PVLGWFSQSV DYGLNESMHL ITKQLQFLWG VPLIRIFFCD ILSKKLLESQ EPAHAQPASP 430 440 450 460 470 480 QNVLPVKSLL KRAFQKSASV RNILRPVGGK RVDSAEVQKV CNICVLYQTS LTTLTQIRLQ 490 500 510 520 530 540 ILTGLTYLDD LLPKLWAFIC ELGPHGGLKL FLECLNNDTE ESKQLLAMLM LFCDCSRHLI 550 560 570 580 590 600 TILDDIEVYE EQISFKLEEL VTISSFLNSF VFKMIWDGIV ENAKGETLEL FQSVHGWLMV 610 620 630 640 650 660 LYERDCRRRF TPEDHWLRKD LKPSVLFQEL DRDRKRAQLI LQYIPHVIPH KNRVLLFRTM 670 680 690 700 VTKEKEKLGL VETSSASPHV THITIRRSRM LESLFECPWP LVINAESC
	Isoform 3 [UniParc]. Checksum: C2B67EC118442978	FASTA	244	28,934
	10 20 30 40 50 60 MFTLSQTSRA WFIDRARQAR EERLVQKERE RAAVVIQAHV RSFLCRSRLQ RDIRREIDDF 70 80 90 100 110 120 FKADDPESTK RSALCIFKIA RKLLFLFRIK EDNERFEKLC RSILSSMDAE NEPKVWYVSL 130 140 150 160 170 180 ACSKDLTLLW IQQIKNILWY CCDFLKQLKP EILQDSRLIT LYLTMLVTFT DTSTWKILRG 190 200 210 220 230 240 KGESLRPAMN HICANIMGHL NQHGFYSVLQ CCDGLFPDLV SYAPHNNPVR WSVGRSWYDW QLSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the human UBE3B gene: structure, expression, evolution, and alternative splicing." Gong T.-W.L., Huang L., Warner S.J., Lomax M.I. Genomics 82:143-152(2003) [PubMed: 12837265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT GLN-346. Tissue: Endometrial adenocarcinoma.
[3]	"The finished DNA sequence of human chromosome 12." Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A. Nature 440:346-351(2006) [PubMed: 16541075] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), VARIANT GLN-346. Tissue: Brain, Lung, Prostate and Skin.
[5]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-419, MASS SPECTROMETRY. Tissue: Cervix carcinoma.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF251046 mRNA. Translation: AAK28419.2. AL096740 mRNA. Translation: CAH56410.1. BX537403 mRNA. Translation: CAD97645.1. AC007570 Genomic DNA. No translation available. BC032301 mRNA. Translation: AAH32301.1. BC051266 mRNA. Translation: AAH51266.1. BC068221 mRNA. Translation: AAH68221.1. BC108705 mRNA. Translation: AAI08706.1. BC141880 mRNA. Translation: AAI41881.1.
IPI	IPI00385576. IPI00411596. IPI00470597.
RefSeq	NP_569733.2. NM_130466.2. NP_904324.1. NM_183415.1.
UniGene	Hs.374067.
3D structure databases
HSSP	HSSP built from PDB template 1ND7 based on UniProtKB Q9H0M0.
ProteinModelPortal	Q7Z3V4.
SMR	Q7Z3V4. Positions 637-1061.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q7Z3V4. 1 interaction.
STRING	Q7Z3V4.
PTM databases
PhosphoSite	Q7Z3V4.
Polymorphism databases
DMDM	296453010.
Proteomic databases
PRIDE	Q7Z3V4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000342494; ENSP00000340596; ENSG00000151148. ENST00000434735; ENSP00000391529; ENSG00000151148.
GeneID	89910.
KEGG	hsa:89910.
UCSC	uc001tom.2. human. uc009zvj.1. human.
Organism-specific databases
CTD	89910.
GeneCards	GC12P109915.
H-InvDB	HIX0020836.
HGNC	HGNC:13478. UBE3B.
HPA	HPA041012.
MIM	608047. gene.
neXtProt	NX_Q7Z3V4.
PharmGKB	PA134872189.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG10089.
GeneTree	ENSGT00550000074668.
HOGENOM	HBG315289.
HOVERGEN	HBG108644.
InParanoid	Q7Z3V4.
OMA	FFTIRKR.
OrthoDB	EOG4R501Z.
PhylomeDB	Q7Z3V4.
Enzyme and pathway databases
Reactome	REACT_6900. Immune System.
Gene expression databases
ArrayExpress	Q7Z3V4.
Bgee	Q7Z3V4.
CleanEx	HS_UBE3B.
Genevestigator	Q7Z3V4.
Family and domain databases
InterPro	IPR000569. HECT. IPR000048. IQ_motif_EF-hand-BS. [Graphical view]
KO	K10588.
Pfam	PF00632. HECT. 1 hit. PF00612. IQ. 1 hit. [Graphical view]
SMART	SM00119. HECTc. 1 hit. SM00015. IQ. 1 hit. [Graphical view]
SUPFAM	SSF56204. HECT. 1 hit.
PROSITE	PS50237. HECT. 1 hit. PS50096. IQ. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	76429.
SOURCE	Search...

Entry information

Entry name

UBE3B_HUMAN

Accession

Primary (citable) accession number: Q7Z3V4
Secondary accession number(s): A5D8Z3 , Q05BX9, Q659F7, Q7Z7Q1, Q9BXZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	May 18, 2010
Last modified:	January 25, 2012
This is version 67 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.