##gff-version 3
Q7Z3F1	UniProtKB	Chain	1	870	.	.	.	ID=PRO_0000087551;Note=Lysosomal cholesterol signaling protein	
Q7Z3F1	UniProtKB	Transmembrane	39	59	.	.	.	Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255	
Q7Z3F1	UniProtKB	Transmembrane	80	100	.	.	.	Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255	
Q7Z3F1	UniProtKB	Transmembrane	105	125	.	.	.	Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255	
Q7Z3F1	UniProtKB	Transmembrane	134	154	.	.	.	Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255	
Q7Z3F1	UniProtKB	Transmembrane	168	188	.	.	.	Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255	
Q7Z3F1	UniProtKB	Transmembrane	214	234	.	.	.	Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255	
Q7Z3F1	UniProtKB	Transmembrane	244	264	.	.	.	Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255	
Q7Z3F1	UniProtKB	Transmembrane	274	294	.	.	.	Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255	
Q7Z3F1	UniProtKB	Transmembrane	316	336	.	.	.	Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255	
Q7Z3F1	UniProtKB	Transmembrane	347	367	.	.	.	Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255	
Q7Z3F1	UniProtKB	Transmembrane	382	402	.	.	.	Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255	
Q7Z3F1	UniProtKB	Transmembrane	415	435	.	.	.	Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255	
Q7Z3F1	UniProtKB	Transmembrane	439	459	.	.	.	Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255	
Q7Z3F1	UniProtKB	Transmembrane	481	501	.	.	.	Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255	
Q7Z3F1	UniProtKB	Transmembrane	521	541	.	.	.	Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255	
Q7Z3F1	UniProtKB	Transmembrane	661	681	.	.	.	Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255	
Q7Z3F1	UniProtKB	Transmembrane	692	712	.	.	.	Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255	
Q7Z3F1	UniProtKB	Domain	757	835	.	.	.	Note=DEP;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00066	
Q7Z3F1	UniProtKB	Binding site	43	43	.	.	.	Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:36007018;Dbxref=PMID:36007018	
Q7Z3F1	UniProtKB	Binding site	57	57	.	.	.	Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:36007018;Dbxref=PMID:36007018	
Q7Z3F1	UniProtKB	Glycosylation	9	9	.	.	.	Note=N-linked (GlcNAc...) asparagine;Ontology_term=ECO:0000255;evidence=ECO:0000255	
Q7Z3F1	UniProtKB	Glycosylation	15	15	.	.	.	Note=N-linked (GlcNAc...) asparagine;Ontology_term=ECO:0000255;evidence=ECO:0000255	
Q7Z3F1	UniProtKB	Glycosylation	29	29	.	.	.	Note=N-linked (GlcNAc...) asparagine;Ontology_term=ECO:0000255;evidence=ECO:0000255	
Q7Z3F1	UniProtKB	Glycosylation	198	198	.	.	.	Note=N-linked (GlcNAc...) asparagine;Ontology_term=ECO:0000255;evidence=ECO:0000255	
Q7Z3F1	UniProtKB	Glycosylation	309	309	.	.	.	Note=N-linked (GlcNAc...) asparagine;Ontology_term=ECO:0000255;evidence=ECO:0000255	
Q7Z3F1	UniProtKB	Glycosylation	381	381	.	.	.	Note=N-linked (GlcNAc...) asparagine;Ontology_term=ECO:0000255;evidence=ECO:0000255	
Q7Z3F1	UniProtKB	Glycosylation	543	543	.	.	.	Note=N-linked (GlcNAc...) asparagine;Ontology_term=ECO:0000255;evidence=ECO:0000255	
Q7Z3F1	UniProtKB	Glycosylation	612	612	.	.	.	Note=N-linked (GlcNAc...) asparagine;Ontology_term=ECO:0000255;evidence=ECO:0000255	
Q7Z3F1	UniProtKB	Mutagenesis	43	44	.	.	.	Note=Nearly abolished cholesterol-binding. FP->IA;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36007018;Dbxref=PMID:36007018	
Q7Z3F1	UniProtKB	Mutagenesis	43	43	.	.	.	Note=Strongly reduced cholesterol-binding. F->I;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36007018;Dbxref=PMID:36007018	
Q7Z3F1	UniProtKB	Mutagenesis	48	48	.	.	.	Note=Does not affect cholesterol-binding. E->Q;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36007018;Dbxref=PMID:36007018	
Q7Z3F1	UniProtKB	Mutagenesis	57	57	.	.	.	Note=Strongly reduced cholesterol-binding. Y->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36007018;Dbxref=PMID:36007018	
Q7Z3F1	UniProtKB	Mutagenesis	551	551	.	.	.	Note=Abolished ability to regulate mTORC1. Y->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36007018;Dbxref=PMID:36007018	
Q7Z3F1	UniProtKB	Mutagenesis	595	595	.	.	.	Note=In 4CA%2C abolished ability to regulate mTORC1%3B when associated with A-604%2C A-629 and A-638. C->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36007018;Dbxref=PMID:36007018	
Q7Z3F1	UniProtKB	Mutagenesis	604	604	.	.	.	Note=In 4CA%2C abolished ability to regulate mTORC1%3B when associated with A-595%2C A-629 and A-638. C->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36007018;Dbxref=PMID:36007018	
Q7Z3F1	UniProtKB	Mutagenesis	629	629	.	.	.	Note=In 4CA%2C abolished ability to regulate mTORC1%3B when associated with A-595%2C A-604 and A-638. C->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36007018;Dbxref=PMID:36007018	
Q7Z3F1	UniProtKB	Mutagenesis	638	638	.	.	.	Note=In 4CA%2C abolished ability to regulate mTORC1%3B when associated with A-595%2C A-604 and A-629. C->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:36007018;Dbxref=PMID:36007018	
Q7Z3F1	UniProtKB	Sequence conflict	33	33	.	.	.	Note=D->G;Ontology_term=ECO:0000305;evidence=ECO:0000305	
Q7Z3F1	UniProtKB	Sequence conflict	194	194	.	.	.	Note=K->E;Ontology_term=ECO:0000305;evidence=ECO:0000305	
Q7Z3F1	UniProtKB	Sequence conflict	260	260	.	.	.	Note=L->H;Ontology_term=ECO:0000305;evidence=ECO:0000305	
Q7Z3F1	UniProtKB	Sequence conflict	460	460	.	.	.	Note=W->R;Ontology_term=ECO:0000305;evidence=ECO:0000305	
Q7Z3F1	UniProtKB	Sequence conflict	517	517	.	.	.	Note=G->A;Ontology_term=ECO:0000305;evidence=ECO:0000305	
Q7Z3F1	UniProtKB	Sequence conflict	550	550	.	.	.	Note=S->G;Ontology_term=ECO:0000305;evidence=ECO:0000305	
Q7Z3F1	UniProtKB	Sequence conflict	638	638	.	.	.	Note=C->R;Ontology_term=ECO:0000305;evidence=ECO:0000305	
Q7Z3F1	UniProtKB	Sequence conflict	674	674	.	.	.	Note=S->Y;Ontology_term=ECO:0000305;evidence=ECO:0000305	
Q7Z3F1	UniProtKB	Sequence conflict	693	693	.	.	.	Note=Q->R;Ontology_term=ECO:0000305;evidence=ECO:0000305