Q7Z2X4 (PCLI1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
PTB-containing, cubilin and LRP1-interacting protein
Short name=P-CLI1

Alternative name(s):
Phosphotyrosine interaction domain-containing protein 1
Protein NYGGF4

Gene names

Name:	PID1
Synonyms:	NYGGF4, PCLI1
ORF Names:	HMFN2073

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Increases proliferation of preadipocytes without affecting adipocytic differentiation. Ref.2
Subunit structure	Found in a complex with PID1/PCLI1, LRP1 and CUBNI. Interacts with LRP1 and CUBN. Ref.6
Subcellular location	Cytoplasm Ref.2.
Tissue specificity	Expressed in subcutaneous fat, heart, skeletal muscle, brain, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocyte. Ref.2
Induction	Up-regulated in fat of obese subjects. Ref.2
Polymorphism	Some sequences seem to have a duplication of exon 2.
Sequence similarities	Contains 1 PID domain.
Sequence caution	The sequence BAD38656.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to fatty acid Inferred from sequence or structural similarity. Source: BHF-UCL cellular response to interleukin-6 Inferred from sequence or structural similarity. Source: BHF-UCL cellular response to leptin stimulus Inferred from electronic annotation. Source: Compara cellular response to tumor necrosis factor Inferred from sequence or structural similarity. Source: BHF-UCL energy reserve metabolic process Inferred by curator PubMed 20165904. Source: BHF-UCL mitochondrion morphogenesis Inferred from mutant phenotype PubMed 20165904. Source: BHF-UCL negative regulation of ATP biosynthetic process Inferred from mutant phenotype PubMed 20165904. Source: BHF-UCL negative regulation of establishment of protein localization to plasma membrane Inferred from direct assay PubMed 19079291. Source: BHF-UCL negative regulation of glucose import in response to insulin stimulus Inferred from direct assay PubMed 19079291. Source: BHF-UCL negative regulation of insulin receptor signaling pathway Inferred from direct assay PubMed 19079291. Source: BHF-UCL negative regulation of mitochondrial DNA replication Inferred from mutant phenotype PubMed 20165904. Source: BHF-UCL negative regulation of protein phosphorylation Inferred from direct assay PubMed 19079291. Source: BHF-UCL positive regulation of ATP biosynthetic process Inferred from sequence or structural similarity. Source: BHF-UCL positive regulation of fat cell proliferation Inferred from direct assay Ref.2. Source: BHF-UCL positive regulation of reactive oxygen species metabolic process Inferred from mutant phenotype PubMed 20165904. Source: BHF-UCL positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 20165904. Source: BHF-UCL regulation of G1/S transition of mitotic cell cycle Inferred from direct assay Ref.2. Source: BHF-UCL regulation of mitochondrial fusion Inferred by curator PubMed 20165904. Source: BHF-UCL regulation of mitochondrial membrane potential Inferred from sequence or structural similarity. Source: BHF-UCL
Cellular_component	mitochondrion Inferred from direct assay. Source: HPA nucleus Inferred from direct assay. Source: HPA
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q7Z2X4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q7Z2X4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-43: MFSLPLSLPL...LIMIFLASGT → MPRIAGNHLM...LHFGLPASEM

Isoform 3 (identifier: Q7Z2X4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-82: Missing.
     83-92: MKTRTHSGCK → MWQPATERLQ

Isoform 4 (identifier: Q7Z2X4-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-43: MFSLPLSLPLCEDTAFLPSKCCSSHKTIKQARTLIMIFLASGT → MWQPATERLQ

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 250	250	PTB-containing, cubilin and LRP1-interacting protein		PRO_0000274900
Regions
	Domain	93 – 250	158	PID
Amino acid modifications
	Modified residue	236	1	Phosphoserine By similarity
	Modified residue	247	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 82	82	Missing in isoform 3.		VSP_022909
	Alternative sequence	1 – 43	43	MFSLP…LASGT → MPRIAGNHLMLEESRTCSSP ELLDGVWPCQPLHFGLPASE M in isoform 2.		VSP_022910
	Alternative sequence	1 – 43	43	MFSLP…LASGT → MWQPATERLQ in isoform 4.		VSP_022911
	Alternative sequence	83 – 92	10	MKTRTHSGCK → MWQPATERLQ in isoform 3.		VSP_022912
	Natural variant	43	1	T → THFQTMLKSKLNVLTLKKEP LPAVIFHEPEAIELCTTTPL MKTRTHSGCK in variant with duplicated exon 2.		VAR_030361

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 3C2F4AE5254D2885	FASTA	250	28,272
	10 20 30 40 50 60 MFSLPLSLPL CEDTAFLPSK CCSSHKTIKQ ARTLIMIFLA SGTHFQTMLK SKLNVLTLKK 70 80 90 100 110 120 EPLPAVIFHE PEAIELCTTT PLMKTRTHSG CKVTYLGKVS TTGMQFLSGC TEKPVIELWK 130 140 150 160 170 180 KHTLAREDVF PANALLEIRP FQVWLHHLDH KGEATVHMDT FQVARIAYCT ADHNVSPNIF 190 200 210 220 230 240 AWVYREINDD LSYQMDCHAV ECESKLEAKK LAHAMMEAFR KTFHSMKSDG RIHSNSSSEE 250 VSQELESDDG
	Isoform 2 [UniParc]. Checksum: D4D89783A12B71D3	FASTA	248	28,121
	10 20 30 40 50 60 MPRIAGNHLM LEESRTCSSP ELLDGVWPCQ PLHFGLPASE MHFQTMLKSK LNVLTLKKEP 70 80 90 100 110 120 LPAVIFHEPE AIELCTTTPL MKTRTHSGCK VTYLGKVSTT GMQFLSGCTE KPVIELWKKH 130 140 150 160 170 180 TLAREDVFPA NALLEIRPFQ VWLHHLDHKG EATVHMDTFQ VARIAYCTAD HNVSPNIFAW 190 200 210 220 230 240 VYREINDDLS YQMDCHAVEC ESKLEAKKLA HAMMEAFRKT FHSMKSDGRI HSNSSSEEVS QELESDDG
	Isoform 3 [UniParc]. Checksum: 5A8DBD3D2B54E512	FASTA	168	19,285
	10 20 30 40 50 60 MWQPATERLQ VTYLGKVSTT GMQFLSGCTE KPVIELWKKH TLAREDVFPA NALLEIRPFQ 70 80 90 100 110 120 VWLHHLDHKG EATVHMDTFQ VARIAYCTAD HNVSPNIFAW VYREINDDLS YQMDCHAVEC 130 140 150 160 ESKLEAKKLA HAMMEAFRKT FHSMKSDGRI HSNSSSEEVS QELESDDG
	Isoform 4 [UniParc]. Checksum: 247256CD547081F5	FASTA	217	24,829
	10 20 30 40 50 60 MWQPATERLQ HFQTMLKSKL NVLTLKKEPL PAVIFHEPEA IELCTTTPLM KTRTHSGCKV 70 80 90 100 110 120 TYLGKVSTTG MQFLSGCTEK PVIELWKKHT LAREDVFPAN ALLEIRPFQV WLHHLDHKGE 130 140 150 160 170 180 ATVHMDTFQV ARIAYCTADH NVSPNIFAWV YREINDDLSY QMDCHAVECE SKLEAKKLAH 190 200 210 AMMEAFRKTF HSMKSDGRIH SNSSSEEVSQ ELESDDG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression profiling and differential screening between hepatoblastomas and the corresponding normal livers: identification of high expression of the PLK1 oncogene as a poor-prognostic indicator of hepatoblastomas." Yamada S., Ohira M., Horie H., Ando K., Takayasu H., Suzuki Y., Sugano S., Hirata T., Goto T., Matsunaga T., Hiyama E., Hayashi Y., Ando H., Suita S., Kaneko M., Sasaki F., Hashizume K., Ohnuma N., Nakagawara A. Oncogene 23:5901-5911(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4). Tissue: Liver.
[2]	"Identification and characterization of NYGGF4, a novel gene containing a phosphotyrosine-binding (PTB) domain that stimulates 3T3-L1 preadipocytes proliferation." Wang B., Zhang M., Ni Y.-H., Liu F., Fan H.-Q., Fei L., Pan X.-Q., Guo M., Chen R.-H., Guo X.-R. Gene 379:132-140(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, INDUCTION, TISSUE SPECIFICITY. Tissue: Adipose tissue.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 4), VARIANT EXON-2 DUPLICATED. Tissue: Brain, Ileal mucosa and Tongue.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Ovary.
[6]	"Identification of the ligands of protein interaction domains through a functional approach." Caratu G., Allegra D., Bimonte M., Schiattarella G.G., D'Ambrosio C., Scaloni A., Napolitano M., Russo T., Zambrano N. Mol. Cell. Proteomics 6:333-345(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH LRP1 AND CUBN, INTERACTION WITH LRP1 AND CUBN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB075874 mRNA. Translation: BAD38656.1. Different initiation. AY317148 mRNA. Translation: AAP79437.1. AK000708 mRNA. Translation: BAA91333.1. AK096636 mRNA. Translation: BAG53344.1. AK125359 mRNA. Translation: BAC86145.1. CH471063 Genomic DNA. Translation: EAW70891.1. BC040164 mRNA. Translation: AAH40164.1.
IPI	IPI00384947. IPI00744724. IPI00792193. IPI00827514.
RefSeq	NP_001094288.1. NM_001100818.1. NP_060403.3. NM_017933.4.
UniGene	Hs.409352.
3D structure databases
ProteinModelPortal	Q7Z2X4.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000375907.
PTM databases
PhosphoSite	Q7Z2X4.
Polymorphism databases
DMDM	74713284.
Proteomic databases
PaxDb	Q7Z2X4.
PeptideAtlas	Q7Z2X4.
PRIDE	Q7Z2X4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000354069; ENSP00000283937; ENSG00000153823. ENST00000392054; ENSP00000375907; ENSG00000153823. ENST00000392055; ENSP00000375908; ENSG00000153823. ENST00000409462; ENSP00000386826; ENSG00000153823.
GeneID	55022.
KEGG	hsa:55022.
UCSC	uc002vpr.4. human. uc002vps.4. human. uc002vpt.4. human. uc002vpu.4. human.
Organism-specific databases
CTD	55022.
GeneCards	GC02M229715.
HGNC	HGNC:26084. PID1.
HPA	HPA036103.
MIM	612930. gene.
neXtProt	NX_Q7Z2X4.
PharmGKB	PA162399462.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG260811.
HOGENOM	HOG000115472.
HOVERGEN	HBG054924.
OMA	TVIFHEP.
PhylomeDB	Q7Z2X4.
Gene expression databases
ArrayExpress	Q7Z2X4.
Bgee	Q7Z2X4.
CleanEx	HS_PID1.
Genevestigator	Q7Z2X4.
Family and domain databases
Gene3D	2.30.29.30. 1 hit.
InterPro	IPR011993. PH_like_dom. IPR006020. PTyr_interaction_dom. [Graphical view]
Pfam	PF00640. PID. 1 hit. [Graphical view]
PROSITE	PS01179. PID. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	PID1. human.
GenomeRNAi	55022.
NextBio	58400.
SOURCE	Search...

Entry information

Entry name

PCLI1_HUMAN

Accession

Primary (citable) accession number: Q7Z2X4
Secondary accession number(s): B3KU82 , Q68CJ2, Q6ZUS3, Q8IXL0, Q9NWP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	October 1, 2003
Last modified:	May 29, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.