Reviewed, UniProtKB/Swiss-Prot Q7Z2D5 (LPPR4_HUMAN)

Names and origin

Protein names

Recommended name:
    Lipid phosphate phosphatase-related protein type 4
    EC=3.1.3.4
Alternative name(s):
    Plasticity-related gene 1 protein
      Short name=PRG-1
    Brain-specific phosphatidic acid phosphatase-like protein 1

Gene names

Name:	LPPR4
Synonyms:	KIAA0455, PRG1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	763 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydrolyzes lysophosphatidic acid (LPA). Facilitates axonal outgrowth during development and regenerative sprouting. In the outgrowing axons acts as an ecto-enzyme and attenuates phospholipid-induced axon collapse in neurons and facilitates outgrowth in the hippocampus. Ref.1
Catalytic activity	A 3-sn-phosphatidate + H2O = a 1,2-diacyl-sn-glycerol + phosphate.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Tissue specificity	Specifically expressed in neurons. Ref.1
Sequence similarities	Belongs to the PA-phosphatase related phosphoesterase family.
Sequence caution	The sequence CAI22799.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence EAW72991.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	phosphatidate phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q7Z2D5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q7Z2D5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     552-577: SARAKWLKAAEKTVACNRSNSQPRIM → RVSSKAAPRTLKAARSPALAPSAIKP
     578-763: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 763	763	Lipid phosphate phosphatase-related protein type 4		PRO_0000317436
Regions
	Transmembrane	67 – 87	21	Potential
	Transmembrane	119 – 139	21	Potential
	Transmembrane	178 – 198	21	Potential
	Transmembrane	247 – 267	21	Potential
	Transmembrane	276 – 296	21	Potential
	Transmembrane	308 – 328	21	Potential
	Compositional bias	695 – 698	4	Poly-His
Amino acid modifications
	Modified residue	217	1	Phosphoserine By similarity
	Modified residue	346	1	Phosphoserine By similarity
	Modified residue	416	1	Phosphothreonine By similarity
	Glycosylation	214	1	N-linked (GlcNAc...) Potential
	Glycosylation	219	1	N-linked (GlcNAc...) Potential
	Glycosylation	268	1	N-linked (GlcNAc...) Potential
	Glycosylation	362	1	N-linked (GlcNAc...) Potential
	Glycosylation	432	1	N-linked (GlcNAc...) Potential
	Glycosylation	455	1	N-linked (GlcNAc...) Potential
	Glycosylation	513	1	N-linked (GlcNAc...) Potential
	Glycosylation	543	1	N-linked (GlcNAc...) Potential
	Glycosylation	568	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	552 – 577	26	SARAK…QPRIM → RVSSKAAPRTLKAARSPALA PSAIKP in isoform 2.		VSP_030950
	Alternative sequence	578 – 763	186	Missing in isoform 2.		VSP_030951
	Natural variant	2	1	Q → K: dbSNP rs712896.		VAR_050618
	Natural variant	32	1	A → V: dbSNP rs35285687.		VAR_050619
Experimental info
	Mutagenesis	252	1	H → K: Loss of activity. Ref.1
	Sequence conflict	18	1	A → S in BAB71245. Ref.4
	Sequence conflict	718	1	L → R in CAD39052. Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: F0401DD5073EBE71	FASTA	763	82,983
	10 20 30 40 50 60 MQRAGSSGGR GECDISGAGR LGLEEAARLS CAVHTSPGGG RRPGQAAGMS AKERPKGKVI 70 80 90 100 110 120 KDSVTLLPCF YFVELPILAS SVVSLYFLEL TDVFKPVHSG FSCYDRSLSM PYIEPTQEAI 130 140 150 160 170 180 PFLMLLSLAF AGPAITIMVG EGILYCCLSK RRNGVGLEPN INAGGCNFNS FLRRAVRFVG 190 200 210 220 230 240 VHVFGLCSTA LITDIIQLST GYQAPYFLTV CKPNYTSLNV SCKENSYIVE DICSGSDLTV 250 260 270 280 290 300 INSGRKSFPS QHATLAAFAA VYVSMYFNST LTDSSKLLKP LLVFTFIICG IICGLTRITQ 310 320 330 340 350 360 YKNHPVDVYC GFLIGGGIAL YLGLYAVGNF LPSDESMFQH RDALRSLTDL NQDPNRLLSA 370 380 390 400 410 420 KNGSSSDGIA HTEGILNRNH RDASSLTNLK RANADVEIIT PRSPMGKENM VTFSNTLPRA 430 440 450 460 470 480 NTPSVEDPVR RNASIHASMD SARSKQLLTQ WKNKNESRKL SLQVIEPEPG QSPPRSIEMR 490 500 510 520 530 540 SSSEPSRVGV NGDHHGPGNQ YLKIQPGAVP GCNNSMPGGP RVSIQSRPGS SQLVHIPEET 550 560 570 580 590 600 QENISTSPKS SSARAKWLKA AEKTVACNRS NSQPRIMQVI AMSKQQGVLQ SSPKNTEGST 610 620 630 640 650 660 VSCTGSIRYK TLTDHEPSGI VRVEAHPENN RPIIQIPSTE GEGSGSWKWK APEKGSLRQT 670 680 690 700 710 720 YELNDLNRDS ESCESLKDSF GSGDRKRSNI DSNEHHHHGI TTIRVTPVEG SEIGSETLSI 730 740 750 760 SSSRDSTLRR KGNIILIPER SNSPENTRNI FYKGTSPTRA YKD
	Isoform 2. Checksum: BB77A61AD5A407E2	FASTA	577	62,148
	10 20 30 40 50 60 MQRAGSSGGR GECDISGAGR LGLEEAARLS CAVHTSPGGG RRPGQAAGMS AKERPKGKVI 70 80 90 100 110 120 KDSVTLLPCF YFVELPILAS SVVSLYFLEL TDVFKPVHSG FSCYDRSLSM PYIEPTQEAI 130 140 150 160 170 180 PFLMLLSLAF AGPAITIMVG EGILYCCLSK RRNGVGLEPN INAGGCNFNS FLRRAVRFVG 190 200 210 220 230 240 VHVFGLCSTA LITDIIQLST GYQAPYFLTV CKPNYTSLNV SCKENSYIVE DICSGSDLTV 250 260 270 280 290 300 INSGRKSFPS QHATLAAFAA VYVSMYFNST LTDSSKLLKP LLVFTFIICG IICGLTRITQ 310 320 330 340 350 360 YKNHPVDVYC GFLIGGGIAL YLGLYAVGNF LPSDESMFQH RDALRSLTDL NQDPNRLLSA 370 380 390 400 410 420 KNGSSSDGIA HTEGILNRNH RDASSLTNLK RANADVEIIT PRSPMGKENM VTFSNTLPRA 430 440 450 460 470 480 NTPSVEDPVR RNASIHASMD SARSKQLLTQ WKNKNESRKL SLQVIEPEPG QSPPRSIEMR 490 500 510 520 530 540 SSSEPSRVGV NGDHHGPGNQ YLKIQPGAVP GCNNSMPGGP RVSIQSRPGS SQLVHIPEET 550 560 570 QENISTSPKS SRVSSKAAPR TLKAARSPAL APSAIKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A new phospholipid phosphatase, PRG-1, is involved in axon growth and regenerative sprouting." Braeuer A.U., Savaskan N.E., Kuehn H., Prehn S., Ninnemann O., Nitsch R. Nat. Neurosci. 6:572-578(2003) [PubMed: 12730698] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF HIS-252. Tissue: Brain.
[2]	"Lipid phosphate phosphatase related proteins." Morris A.J., Sigal Y.J., McDermott M., Sciorra V.A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Characterization of cDNA clones in size-fractionated cDNA libraries from human brain." Seki N., Ohira M., Nagase T., Ishikawa K., Miyajima N., Nakajima D., Nomura N., Ohara O. DNA Res. 4:345-349(1997) [PubMed: 9455484] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 15-763 (ISOFORM 1). Tissue: Brain.
[5]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"A new brain specific member of the PAP-2 family." Ninnemann O., Brauer A.U., Savaskan N., Nitsch R. Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 24-763 (ISOFORM 1).
[8]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 111-763 (ISOFORM 1). Tissue: Amygdala.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF541281 mRNA. Translation: AAP57770.1. AY304518 mRNA. Translation: AAP72155.1. AB007924 mRNA. Translation: BAA32300.3. Different initiation. AK056665 mRNA. Translation: BAB71245.1. AK291369 mRNA. Translation: BAF84058.1. AL139425 Genomic DNA. Translation: CAI22799.1. Sequence problems. AL139425, AL136147 Genomic DNA. Translation: CAI22800.1. AL136147, AL139425 Genomic DNA. Translation: CAI23225.1. CH471097 Genomic DNA. Translation: EAW72991.1. Sequence problems. CH471097 Genomic DNA. Translation: EAW72992.1. AF357013 mRNA. Translation: AAO85400.1. AL834390 mRNA. Translation: CAD39052.1.
IPI	IPI00337643. IPI00844586.
RefSeq	NP_001159724.1. NP_055654.2.
UniGene	Hs.13245
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q7Z2D5.
Proteomic databases
PRIDE	Q7Z2D5.
Genome annotation databases
Ensembl	ENST00000370185; ENSP00000359204; ENSG00000117600; Homo sapiens. [Genome view]
GeneID	9890.
UCSC	uc001dse.1. human.
Organism-specific databases
CTD	9890.
GeneCards	GC01P099442.
HPA	HPA008798.
MIM	607813. gene.
HUGE	Search...
Phylogenomic databases
HOGENOM	HBG443683.
HOVERGEN	Q7Z2D5.
InParanoid	Q7Z2D5.
OMA	SSDGIAH.
OrthoDB	EOG95B40S.
PhylomeDB	Q7Z2D5.
Enzyme and pathway databases
BRENDA	3.1.3.4. 247.
Gene expression databases
ArrayExpress	Q7Z2D5.
Bgee	Q7Z2D5.
Genevestigator	Q7Z2D5.
Family and domain databases
InterPro	IPR000326. P_Acid_Pase_2/haloperoxidase. [Graphical view]
Pfam	PF01569. PAP2. 1 hit. [Graphical view]
SMART	SM00014. acidPPc. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	37288.
SOURCE	Search...

Entry information

Entry name

LPPR4_HUMAN

Accession

Primary (citable) accession number: Q7Z2D5
Secondary accession number(s): O75043 , Q5T9R9, Q86XQ5, Q8N3F1, Q96MP0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 1, 2003
Last modified:	February 9, 2010
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.