Q7Z269 (SP4_POLDO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Venom serine protease EC=3.4.21.- Alternative name(s): Allergen=Pol d 4
Organism	Polistes dominula (European paper wasp) (Vespa dominula)
Taxonomic identifier	34728 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Aculeata › Vespoidea › Vespidae › Polistinae › Polistini › Polistes

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Subcellular location	Secreted By similarity.
Tissue specificity	Expressed by the venom duct.
Allergenic properties	Causes an allergic reaction in human By similarity.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Disease	Allergen
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 277	258	Venom serine protease		PRO_5000090673
Regions
	Domain	34 – 269	236	Peptidase S1
Sites
	Active site	75	1	Charge relay system By similarity
	Active site	126	1	Charge relay system By similarity
	Active site	220	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	84	1	N-linked (GlcNAc...) Potential
	Glycosylation	104	1	N-linked (GlcNAc...) Potential
	Glycosylation	155	1	N-linked (GlcNAc...) Potential
	Glycosylation	158	1	N-linked (GlcNAc...) Potential
	Glycosylation	218	1	N-linked (GlcNAc...) Potential
	Glycosylation	266	1	N-linked (GlcNAc...) Potential
	Disulfide bond	60 ↔ 76		By similarity
	Disulfide bond	192 ↔ 207		By similarity
	Disulfide bond	216 ↔ 246		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7Z269 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 9B316A8824C5CDA6	FASTA	277	30,804
	10 20 30 40 50 60 MNCGKIILLF ITIIGVAKSR EENCKCGWDN PSRIVNGVET EINEFPMVAR LIYPSPGMYC 70 80 90 100 110 120 GGTIITPQHI VTAAHCLQKY KRTNYTGIHV VVGEHDYTTD TETNVTKRYT IAEVTIHPNY 130 140 150 160 170 180 NSHNNDIAIV KTNERFEYSM KVGPVCLPFN YMTRNLTNET VTALGWGKLR YNGQNSKVLR 190 200 210 220 230 240 KVDLHVITRE QCETHYGAAI ANANLLCTFD VGRDACQNDS GGPILWRSPT TDNLILVGVV 250 260 270 NFGRTCADDA PGGNARVTSF MEFIHNATIG ETYCKAD

References

[1]	"Cloning of a paper wasp venom serine protease allergen." Fitch C.D., Hoffman D.R., Schmidt M. J. Allergy Clin. Immunol. 107:S221-S221(2001) Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Hymenoptera venom protease allergens." Winningham K.M., Fitch C.D., Schmidt M., Hoffman D.R. J. Allergy Clin. Immunol. 114:928-933(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Venom gland.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY285998 mRNA. Translation: AAP37412.1.
3D structure databases
HSSP	HSSP built from PDB template 1JBU based on UniProtKB P08709.
ProteinModelPortal	Q7Z269.
ModBase	Search...
Protein family/group databases
Allergome	3437. Pol d 4.0101. 587. Pol d 4.
MEROPS	S01.492.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SP4_POLDO

Accession

Primary (citable) accession number: Q7Z269

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 30, 2010
Last sequence update:	October 1, 2003
Last modified:	March 6, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)