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Q7Z1V1

- CP51_TRYCC

UniProt

Q7Z1V1 - CP51_TRYCC

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Protein

Sterol 14-alpha demethylase

Gene

CYP51

Organism
Trypanosoma cruzi (strain CL Brener)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol (By similarity). Favors C4 dimethylated substrates, the substrate preference order is 24-methylenedihydrolanosterol > 24,25-dihydrolanosterol > lanosterol > obtusifoliol > norlanosterol.By similarity1 Publication

Catalytic activityi

A 14-alpha-methylsteroid + 3 O2 + 3 NADPH = a Delta(14)-steroid + formate + 3 NADP+ + 4 H2O.1 Publication

Cofactori

hemeBy similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi422 – 4221Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. heme binding Source: InterPro
  2. iron ion binding Source: InterPro
  3. sterol 14-demethylase activity Source: UniProtKB-EC

GO - Biological processi

  1. sterol biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00770; UER00754.

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol 14-alpha demethylaseImported1 Publication (EC:1.14.13.70)
Short name:
Tc14DM1 Publication
Alternative name(s):
Cytochrome P450 51By similarity
Lanosterol 14-alpha demethylaseImported1 Publication
Gene namesi
Name:CYP51Imported
ORF Names:Tc00.1047053506297.260, Tc00.1047053510101.50
OrganismiTrypanosoma cruzi (strain CL Brener)
Taxonomic identifieri353153 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosomaSchizotrypanum
ProteomesiUP000002296: Unassembled WGS sequence

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2121HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi105 – 1051I → F: Increases activity on norlanosterol and obtusifoliol. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 481481Sterol 14-alpha demethylasePRO_0000389527Add
BLAST

Expressioni

Developmental stagei

Expressed in both the insect and mammalian life-cycle stages.1 Publication

Interactioni

Structurei

Secondary structure

1
481
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi29 – 313Combined sources
Beta strandi34 – 363Combined sources
Turni40 – 423Combined sources
Helixi45 – 506Combined sources
Helixi52 – 6211Combined sources
Beta strandi66 – 727Combined sources
Beta strandi75 – 806Combined sources
Helixi83 – 853Combined sources
Helixi86 – 905Combined sources
Turni94 – 963Combined sources
Helixi99 – 1024Combined sources
Helixi104 – 1063Combined sources
Helixi107 – 1104Combined sources
Turni112 – 1143Combined sources
Helixi115 – 1173Combined sources
Helixi120 – 13213Combined sources
Helixi136 – 1383Combined sources
Helixi139 – 1413Combined sources
Helixi142 – 15716Combined sources
Beta strandi160 – 1667Combined sources
Helixi167 – 18317Combined sources
Helixi186 – 1916Combined sources
Helixi194 – 20613Combined sources
Helixi210 – 2134Combined sources
Helixi216 – 2205Combined sources
Helixi229 – 25022Combined sources
Turni252 – 2543Combined sources
Helixi261 – 2655Combined sources
Beta strandi271 – 2733Combined sources
Helixi278 – 30831Combined sources
Helixi310 – 3123Combined sources
Helixi313 – 32311Combined sources
Beta strandi324 – 3263Combined sources
Helixi332 – 3376Combined sources
Helixi340 – 35213Combined sources
Beta strandi359 – 3657Combined sources
Beta strandi367 – 3693Combined sources
Beta strandi372 – 3743Combined sources
Beta strandi379 – 3824Combined sources
Helixi384 – 3874Combined sources
Turni391 – 3933Combined sources
Beta strandi394 – 3963Combined sources
Helixi418 – 4203Combined sources
Helixi425 – 44218Combined sources
Beta strandi443 – 4519Combined sources
Beta strandi459 – 4613Combined sources
Helixi466 – 4683Combined sources
Beta strandi470 – 4756Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WUZX-ray2.35A/B22-481[»]
2WX2X-ray2.27A/B22-481[»]
3K1OX-ray2.89A32-481[»]
3KHMX-ray2.85A32-481[»]
3KSWX-ray3.05A32-481[»]
3ZG2X-ray2.80A29-481[»]
3ZG3X-ray2.90A29-481[»]
4BMMX-ray2.84A/B/C/D32-481[»]
4BY0X-ray3.10A/B32-481[»]
4C0CX-ray2.04A32-481[»]
4C27X-ray1.95A/B29-481[»]
4C28X-ray2.03A/B29-481[»]
4CK8X-ray2.62A/B32-481[»]
4CK9X-ray2.74A32-481[»]
4CKAX-ray2.70A32-481[»]
4COHX-ray2.08A/B29-481[»]
4H6OX-ray2.80A29-481[»]
4UQHX-ray2.43A32-481[»]
ProteinModelPortaliQ7Z1V1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7Z1V1.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Sequence Analysis

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK05917.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00465. EP450IV.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7Z1V1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFIEAIVLAL TALILYSVYS VKSFNTTRPT DPPVYPVTVP FLGHIVQFGK
60 70 80 90 100
NPLEFMQRCK RDLKSGVFTI SIGGQRVTIV GDPHEHSRFF SPRNEILSPR
110 120 130 140 150
EVYTIMTPVF GEGVAYAAPY PRMREQLNFL AEELTIAKFQ NFVPAIQHEV
160 170 180 190 200
RKFMAENWKE DEGVINLLED CGAMIINTAC QCLFGEDLRK RLNARHFAQL
210 220 230 240 250
LSKMESSLIP AAVFMPWLLR LPLPQSARCR EARAELQKIL GEIIVAREKE
260 270 280 290 300
EASKDNNTSD LLGGLLKAVY RDGTRMSLHE VCGMIVAAMF AGQHTSTITT
310 320 330 340 350
SWSMLHLMHP KNKKWLDKLH KEIDEFPAQL NYDNVMDEMP FAERCVRESI
360 370 380 390 400
RRDPPLLMVM RMVKAEVKVG SYVVPKGDII ACSPLLSHHD EEAFPNPRLW
410 420 430 440 450
DPERDEKVDG AFIGFGAGVH KCIGQKFALL QVKTILATAF REYDFQLLRD
460 470 480
EVPDPDYHTM VVGPTLNQCL VKYTRKKKLP S
Length:481
Mass (Da):54,683
Last modified:October 1, 2003 - v1
Checksum:iC83BA5243C959151
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91A → G in AAW47718. (PubMed:16321980)Curated
Sequence conflicti9 – 91A → G in EAN98359. (PubMed:16020725)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti62 – 621D → E in allele 2. 2 Publications
Natural varianti117 – 1171A → S in allele 2. 2 Publications
Natural varianti160 – 1601E → K in allele 2. 2 Publications

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY283022 Genomic DNA. Translation: AAP33131.1.
AY283023 Genomic DNA. Translation: AAP33132.1.
AY856083 Genomic DNA. Translation: AAW47718.1.
AAHK01000021 Genomic DNA. Translation: EAN99368.1.
AAHK01000058 Genomic DNA. Translation: EAN98359.1.
RefSeqiXP_820210.1. XM_815117.1.
XP_821219.1. XM_816126.1.

Genome annotation databases

GeneIDi3552837.
3554116.
KEGGitcr:506297.260.
tcr:510101.50.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY283022 Genomic DNA. Translation: AAP33131.1 .
AY283023 Genomic DNA. Translation: AAP33132.1 .
AY856083 Genomic DNA. Translation: AAW47718.1 .
AAHK01000021 Genomic DNA. Translation: EAN99368.1 .
AAHK01000058 Genomic DNA. Translation: EAN98359.1 .
RefSeqi XP_820210.1. XM_815117.1.
XP_821219.1. XM_816126.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2WUZ X-ray 2.35 A/B 22-481 [» ]
2WX2 X-ray 2.27 A/B 22-481 [» ]
3K1O X-ray 2.89 A 32-481 [» ]
3KHM X-ray 2.85 A 32-481 [» ]
3KSW X-ray 3.05 A 32-481 [» ]
3ZG2 X-ray 2.80 A 29-481 [» ]
3ZG3 X-ray 2.90 A 29-481 [» ]
4BMM X-ray 2.84 A/B/C/D 32-481 [» ]
4BY0 X-ray 3.10 A/B 32-481 [» ]
4C0C X-ray 2.04 A 32-481 [» ]
4C27 X-ray 1.95 A/B 29-481 [» ]
4C28 X-ray 2.03 A/B 29-481 [» ]
4CK8 X-ray 2.62 A/B 32-481 [» ]
4CK9 X-ray 2.74 A 32-481 [» ]
4CKA X-ray 2.70 A 32-481 [» ]
4COH X-ray 2.08 A/B 29-481 [» ]
4H6O X-ray 2.80 A 29-481 [» ]
4UQH X-ray 2.43 A 32-481 [» ]
ProteinModelPortali Q7Z1V1.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi Q7Z1V1.
ChEMBLi CHEMBL1075110.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3552837.
3554116.
KEGGi tcr:506297.260.
tcr:510101.50.

Phylogenomic databases

KOi K05917.

Enzyme and pathway databases

UniPathwayi UPA00770 ; UER00754 .

Miscellaneous databases

EvolutionaryTracei Q7Z1V1.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00465. EP450IV.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and analysis of Trypanosoma cruzi lanosterol 14alpha-demethylase."
    Buckner F.S., Joubert B.M., Boyle S.M., Eastman R.T., Verlinde C.L.M.J., Matsuda S.P.T.
    Mol. Biochem. Parasitol. 132:75-81(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELES 1 AND 2), DEVELOPMENTAL STAGE.
    Strain: TulahuenImported.
  2. "CYP51 from Trypanosoma cruzi: a phyla-specific residue in the B' helix defines substrate preferences of sterol 14alpha-demethylase."
    Lepesheva G.I., Zaitseva N.G., Nes W.D., Zhou W., Arase M., Liu J., Hill G.C., Waterman M.R.
    J. Biol. Chem. 281:3577-3585(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELE 1), FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF ILE-105.
  3. "The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease."
    El-Sayed N.M.A., Myler P.J., Bartholomeu D.C., Nilsson D., Aggarwal G., Tran A.-N., Ghedin E., Worthey E.A., Delcher A.L., Blandin G., Westenberger S.J., Caler E., Cerqueira G.C., Branche C., Haas B., Anupama A., Arner E., Aslund L.
    , Attipoe P., Bontempi E., Bringaud F., Burton P., Cadag E., Campbell D.A., Carrington M., Crabtree J., Darban H., da Silveira J.F., de Jong P., Edwards K., Englund P.T., Fazelina G., Feldblyum T., Ferella M., Frasch A.C., Gull K., Horn D., Hou L., Huang Y., Kindlund E., Klingbeil M., Kluge S., Koo H., Lacerda D., Levin M.J., Lorenzi H., Louie T., Machado C.R., McCulloch R., McKenna A., Mizuno Y., Mottram J.C., Nelson S., Ochaya S., Osoegawa K., Pai G., Parsons M., Pentony M., Pettersson U., Pop M., Ramirez J.L., Rinta J., Robertson L., Salzberg S.L., Sanchez D.O., Seyler A., Sharma R., Shetty J., Simpson A.J., Sisk E., Tammi M.T., Tarleton R., Teixeira S., Van Aken S., Vogt C., Ward P.N., Wickstead B., Wortman J., White O., Fraser C.M., Stuart K.D., Andersson B.
    Science 309:409-415(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ALLELES 1 AND 2).
    Strain: CL BrenerImported.

Entry informationi

Entry nameiCP51_TRYCC
AccessioniPrimary (citable) accession number: Q7Z1V1
Secondary accession number(s): Q5I4E1, Q7Z1V0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: October 1, 2003
Last modified: November 26, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3