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Q7Z1V1

- CP51_TRYCC

UniProt

Q7Z1V1 - CP51_TRYCC

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Protein

Sterol 14-alpha demethylase

Gene
CYP51, Tc00.1047053506297.260, Tc00.1047053510101.50
Organism
Trypanosoma cruzi (strain CL Brener)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol By similarity. Favors C4 dimethylated substrates, the substrate preference order is 24-methylenedihydrolanosterol > 24,25-dihydrolanosterol > lanosterol > obtusifoliol > norlanosterol.By similarity1 Publication

Catalytic activityi

A 14-alpha-methylsteroid + 3 O2 + 3 NADPH = a Delta(14)-steroid + formate + 3 NADP+ + 4 H2O.1 Publication

Cofactori

Heme group By similarity.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi422 – 4221Iron (heme axial ligand) By similarityBy similarity

GO - Molecular functioni

  1. heme binding Source: InterPro
  2. iron ion binding Source: InterPro
  3. sterol 14-demethylase activity Source: UniProtKB-EC

GO - Biological processi

  1. sterol biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00770; UER00754.

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol 14-alpha demethylase (EC:1.14.13.70)
Short name:
Tc14DM
Alternative name(s):
Cytochrome P450 51
Lanosterol 14-alpha demethylase
Gene namesi
Name:CYP51
ORF Names:Tc00.1047053506297.260, Tc00.1047053510101.50
OrganismiTrypanosoma cruzi (strain CL Brener)
Taxonomic identifieri353153 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosomaSchizotrypanum
ProteomesiUP000002296: Unassembled WGS sequence

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2121Helical; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi105 – 1051I → F: Increases activity on norlanosterol and obtusifoliol. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 481481Sterol 14-alpha demethylasePRO_0000389527Add
BLAST

Expressioni

Developmental stagei

Expressed in both the insect and mammalian life-cycle stages.1 Publication

Interactioni

Structurei

Secondary structure

1
481
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi29 – 313
Beta strandi34 – 363
Turni40 – 423
Helixi45 – 506
Helixi52 – 6211
Beta strandi66 – 727
Beta strandi75 – 806
Helixi83 – 853
Helixi86 – 905
Turni94 – 963
Beta strandi97 – 993
Helixi100 – 1067
Helixi107 – 1104
Turni112 – 1143
Helixi115 – 1173
Helixi120 – 13213
Helixi136 – 1383
Helixi139 – 1413
Helixi142 – 15716
Beta strandi160 – 1667
Helixi167 – 18317
Helixi186 – 1916
Helixi194 – 20613
Helixi210 – 2134
Helixi216 – 2205
Helixi229 – 24820
Turni249 – 2546
Helixi261 – 2666
Beta strandi271 – 2733
Helixi278 – 30831
Helixi310 – 3123
Helixi313 – 32311
Beta strandi324 – 3263
Helixi332 – 3376
Helixi340 – 35213
Beta strandi359 – 3657
Beta strandi367 – 3693
Beta strandi372 – 3743
Beta strandi379 – 3824
Helixi384 – 3874
Turni391 – 3933
Beta strandi394 – 3963
Helixi418 – 4203
Helixi425 – 44218
Beta strandi443 – 4519
Beta strandi459 – 4613
Helixi466 – 4683
Beta strandi470 – 4767

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WUZX-ray2.35A/B22-481[»]
2WX2X-ray2.27A/B22-481[»]
3K1OX-ray2.89A32-481[»]
3KHMX-ray2.85A32-481[»]
3KSWX-ray3.05A32-481[»]
3ZG2X-ray2.80A29-481[»]
3ZG3X-ray2.90A29-481[»]
4BMMX-ray2.84A/B/C/D32-481[»]
4BY0X-ray3.10A/B32-481[»]
4COHX-ray2.08A/B29-481[»]
4H6OX-ray2.80A29-481[»]
ProteinModelPortaliQ7Z1V1.

Miscellaneous databases

EvolutionaryTraceiQ7Z1V1.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK05917.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00465. EP450IV.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7Z1V1-1 [UniParc]FASTAAdd to Basket

« Hide

MFIEAIVLAL TALILYSVYS VKSFNTTRPT DPPVYPVTVP FLGHIVQFGK    50
NPLEFMQRCK RDLKSGVFTI SIGGQRVTIV GDPHEHSRFF SPRNEILSPR 100
EVYTIMTPVF GEGVAYAAPY PRMREQLNFL AEELTIAKFQ NFVPAIQHEV 150
RKFMAENWKE DEGVINLLED CGAMIINTAC QCLFGEDLRK RLNARHFAQL 200
LSKMESSLIP AAVFMPWLLR LPLPQSARCR EARAELQKIL GEIIVAREKE 250
EASKDNNTSD LLGGLLKAVY RDGTRMSLHE VCGMIVAAMF AGQHTSTITT 300
SWSMLHLMHP KNKKWLDKLH KEIDEFPAQL NYDNVMDEMP FAERCVRESI 350
RRDPPLLMVM RMVKAEVKVG SYVVPKGDII ACSPLLSHHD EEAFPNPRLW 400
DPERDEKVDG AFIGFGAGVH KCIGQKFALL QVKTILATAF REYDFQLLRD 450
EVPDPDYHTM VVGPTLNQCL VKYTRKKKLP S 481
Length:481
Mass (Da):54,683
Last modified:October 1, 2003 - v1
Checksum:iC83BA5243C959151
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti62 – 621D → E in allele 2. 2 Publications
Natural varianti117 – 1171A → S in allele 2. 2 Publications
Natural varianti160 – 1601E → K in allele 2. 2 Publications

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91A → G in AAW47718. 1 Publication
Sequence conflicti9 – 91A → G in EAN98359. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY283022 Genomic DNA. Translation: AAP33131.1.
AY283023 Genomic DNA. Translation: AAP33132.1.
AY856083 Genomic DNA. Translation: AAW47718.1.
AAHK01000021 Genomic DNA. Translation: EAN99368.1.
AAHK01000058 Genomic DNA. Translation: EAN98359.1.
RefSeqiXP_820210.1. XM_815117.1.
XP_821219.1. XM_816126.1.

Genome annotation databases

GeneIDi3552837.
3554116.
KEGGitcr:506297.260.
tcr:510101.50.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY283022 Genomic DNA. Translation: AAP33131.1 .
AY283023 Genomic DNA. Translation: AAP33132.1 .
AY856083 Genomic DNA. Translation: AAW47718.1 .
AAHK01000021 Genomic DNA. Translation: EAN99368.1 .
AAHK01000058 Genomic DNA. Translation: EAN98359.1 .
RefSeqi XP_820210.1. XM_815117.1.
XP_821219.1. XM_816126.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2WUZ X-ray 2.35 A/B 22-481 [» ]
2WX2 X-ray 2.27 A/B 22-481 [» ]
3K1O X-ray 2.89 A 32-481 [» ]
3KHM X-ray 2.85 A 32-481 [» ]
3KSW X-ray 3.05 A 32-481 [» ]
3ZG2 X-ray 2.80 A 29-481 [» ]
3ZG3 X-ray 2.90 A 29-481 [» ]
4BMM X-ray 2.84 A/B/C/D 32-481 [» ]
4BY0 X-ray 3.10 A/B 32-481 [» ]
4COH X-ray 2.08 A/B 29-481 [» ]
4H6O X-ray 2.80 A 29-481 [» ]
ProteinModelPortali Q7Z1V1.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi Q7Z1V1.
ChEMBLi CHEMBL1075110.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3552837.
3554116.
KEGGi tcr:506297.260.
tcr:510101.50.

Phylogenomic databases

KOi K05917.

Enzyme and pathway databases

UniPathwayi UPA00770 ; UER00754 .

Miscellaneous databases

EvolutionaryTracei Q7Z1V1.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00465. EP450IV.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and analysis of Trypanosoma cruzi lanosterol 14alpha-demethylase."
    Buckner F.S., Joubert B.M., Boyle S.M., Eastman R.T., Verlinde C.L.M.J., Matsuda S.P.T.
    Mol. Biochem. Parasitol. 132:75-81(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELES 1 AND 2), DEVELOPMENTAL STAGE.
    Strain: Tulahuen.
  2. "CYP51 from Trypanosoma cruzi: a phyla-specific residue in the B' helix defines substrate preferences of sterol 14alpha-demethylase."
    Lepesheva G.I., Zaitseva N.G., Nes W.D., Zhou W., Arase M., Liu J., Hill G.C., Waterman M.R.
    J. Biol. Chem. 281:3577-3585(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELE 1), FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF ILE-105.
  3. "The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease."
    El-Sayed N.M.A., Myler P.J., Bartholomeu D.C., Nilsson D., Aggarwal G., Tran A.-N., Ghedin E., Worthey E.A., Delcher A.L., Blandin G., Westenberger S.J., Caler E., Cerqueira G.C., Branche C., Haas B., Anupama A., Arner E., Aslund L.
    , Attipoe P., Bontempi E., Bringaud F., Burton P., Cadag E., Campbell D.A., Carrington M., Crabtree J., Darban H., da Silveira J.F., de Jong P., Edwards K., Englund P.T., Fazelina G., Feldblyum T., Ferella M., Frasch A.C., Gull K., Horn D., Hou L., Huang Y., Kindlund E., Klingbeil M., Kluge S., Koo H., Lacerda D., Levin M.J., Lorenzi H., Louie T., Machado C.R., McCulloch R., McKenna A., Mizuno Y., Mottram J.C., Nelson S., Ochaya S., Osoegawa K., Pai G., Parsons M., Pentony M., Pettersson U., Pop M., Ramirez J.L., Rinta J., Robertson L., Salzberg S.L., Sanchez D.O., Seyler A., Sharma R., Shetty J., Simpson A.J., Sisk E., Tammi M.T., Tarleton R., Teixeira S., Van Aken S., Vogt C., Ward P.N., Wickstead B., Wortman J., White O., Fraser C.M., Stuart K.D., Andersson B.
    Science 309:409-415(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ALLELES 1 AND 2).
    Strain: CL Brener.

Entry informationi

Entry nameiCP51_TRYCC
AccessioniPrimary (citable) accession number: Q7Z1V1
Secondary accession number(s): Q5I4E1, Q7Z1V0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: October 1, 2003
Last modified: September 3, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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