Q7Z1K3 (ANIS1_ANISI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Major allergen Ani s 1 Alternative name(s): 21 kDa allergen Excretory gland allergen Ans1 Allergen=Ani s 1
Organism	Anisakis simplex (Herring worm)
Taxonomic identifier	6269 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Ascaridida › Ascaridoidea › Anisakidae › Anisakis › Anisakis simplex complex

Protein attributes

Sequence length	194 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Secreted Ref.2.
Allergenic properties	Causes an allergic reaction in human. Binds to IgE. A.simplex is a fish parasite that, when accidentally ingested by humans, may cause allergic reactions in sensitized individuals. Ref.2
Sequence similarities	Contains 1 BPTI/Kunitz inhibitor domain. Contains 1 WR1 domain.

Ontologies

Keywords
Cellular component	Secreted
Disease	Allergen
Domain	Signal
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Ref.2
	Chain	24 – 194	171	Major allergen Ani s 1		PRO_0000016888
Regions
	Domain	28 – 84	57	BPTI/Kunitz inhibitor
	Domain	90 – 134	45	WR1
Amino acid modifications
	Disulfide bond	28 ↔ 84		By similarity
	Disulfide bond	38 ↔ 67		By similarity
	Disulfide bond	59 ↔ 80		By similarity
Experimental info
	Sequence conflict	37	1	P → Q AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q7Z1K3 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: F1ADA1A463633B37	FASTA	194	21,185
	10 20 30 40 50 60 MASMQHFSLA ALLLAASICL GDADRTECQL PLDKGTPCTQ EGGVKPSVAW WHDDKSGICL 70 80 90 100 110 120 SFKYTGCGGN ANRFTTIKNC EQHCKMPDRG ACALGKKPAE DSNGEQLVCA GMREDKCPNG 130 140 150 160 170 180 YQCKMMAFMG LCCPTKEEEL FAREYEGVCK SGKPVKMDRG SGWMMTILGK SCDDQFCPED 190 AKCERGKLFA NCCK

References

[1]	"Purification and molecular cloning of a majpr allergen from Anisakis simplex." Shimakura K., Miura H., Ikeda K., Ishizaki S., Nagashima Y., Shiomi K., Shirai T., Kasuya S. Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Isolation and characterization of a major allergen from the fish parasite Anisakis simplex." Moneo I., Caballero M.L., Gomez F., Ortega E., Alonso M.J. J. Allergy Clin. Immunol. 106:177-182(2000) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 24-40, SUBCELLULAR LOCATION, ALLERGEN.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB100095 mRNA. Translation: BAC77154.1.
PIR	A59069.
3D structure databases
ProteinModelPortal	Q7Z1K3.
ModBase	Search...
Protein family/group databases
Allergome	3079. Ani s 1.0101. 35. Ani s 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	4.10.410.10. 1 hit.
InterPro	IPR006150. Cys_repeat_1. IPR002223. Prot_inh_Kunz-m. IPR020901. Prtase_inh_Kunz-CS. [Graphical view]
Pfam	PF00014. Kunitz_BPTI. 1 hit. [Graphical view]
PRINTS	PR00759. BASICPTASE.
SMART	SM00131. KU. 1 hit. SM00289. WR1. 2 hits. [Graphical view]
SUPFAM	SSF57362. Prot_inh_Kunz-m. 1 hit.
PROSITE	PS00280. BPTI_KUNITZ_1. 1 hit. PS50279. BPTI_KUNITZ_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ANIS1_ANISI

Accession

Primary (citable) accession number: Q7Z1K3
Secondary accession number(s): Q7M4A7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	October 1, 2003
Last modified:	March 6, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)