Q7Z091 (CI1BD_CONRA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Iota-conotoxin-like r11d Alternative name(s): R11.8
Organism	Conus radiatus (Rayed cone)
Taxonomic identifier	61198 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Lophotrochozoa › Mollusca › Gastropoda › Caenogastropoda › Hypsogastropoda › Neogastropoda › Conoidea › Conidae › Conus

Protein attributes

Sequence length	41 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Iota-conotoxins bind to voltage-gated sodium channels (Nav) and act as agonists by shifting the voltage-dependence of activation to more hyperpolarized levels By similarity. Both natural (L-Thr form) and synthetic (D-Thr form) peptides cause paralysis and death following intracranial injection and grooming and hypersensitivity upon intraperitoneal injection into mice. The L-Thr form of the peptide is 7-fold more potent than the D-Thr form. Both natural peptide (L-Thr form) and synthetic peptide (D-Thr form) are active on nerve, and on muscle. Ref.2
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom duct.
Domain	The cysteine framework is XI (C-C-CC-CC-C-C).
Post-translational modification	Position 41 corresponds to a L-threonine, and not a D-threonine as firstly supposed.
Sequence similarities	Belongs to the conotoxin I1 superfamily.
Mass spectrometry	Molecular mass is 4399.26 Da from positions 1 - 41. Determined by MALDI. Ref.1

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Ion channel impairing toxin Neurotoxin Sodium channel inhibitor Toxin
PTM	Bromination Disulfide bond Hydroxylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sodium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 41	41	Iota-conotoxin-like r11d		PRO_0000086870
Amino acid modifications
	Modified residue	8	1	4-hydroxyproline
	Modified residue	26	1	4-hydroxyproline
	Modified residue	30	1	6'-bromotryptophan
	Disulfide bond	2 ↔ 16		By similarity
	Disulfide bond	9 ↔ 19		By similarity
	Disulfide bond	15 ↔ 24		By similarity
	Disulfide bond	18 ↔ 35		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7Z091 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: B1AD320F1A1B5B31	FASTA	41	4,373
	10 20 30 40 GCKKDRKPCS YHADCCNCCL SGICAPSTNW ILPGCSTSTF T

References

[1]	"Novel excitatory Conus peptides define a new conotoxin superfamily." Jimenez E.C., Shetty R.P., Lirazan M., Rivier J., Walker C., Abogadie F.C., Yoshikami D., Cruz L.J., Olivera B.M. J. Neurochem. 85:610-621(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE, MASS SPECTROMETRY. Tissue: Venom and Venom duct.
[2]	"I(1)-superfamily conotoxins and prediction of single D-amino acid occurrence." Buczek O., Jimenez E.C., Yoshikami D., Imperial J.S., Watkins M., Morrison A., Olivera B.M. Toxicon 51:218-229(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, SYNTHESIS OF [L-THR-41]R11D AND [D-THR-41]R11D, FUNCTION. Tissue: Venom.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY208962 mRNA. Translation: AAP41544.1.
3D structure databases
ProteinModelPortal	Q7Z091.
SMR	Q7Z091. Positions 2-40.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
ConoServer	843. RXID.
Family and domain databases
InterPro	IPR013141. Conotoxin-I_CS. IPR012624. Toxin_19. [Graphical view]
Pfam	PF08088. Toxin_19. 1 hit. [Graphical view]
PROSITE	PS60019. I_CONOTOXIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CI1BD_CONRA

Accession

Primary (citable) accession number: Q7Z091

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 2, 2004
Last sequence update:	October 1, 2003
Last modified:	May 1, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program