Q7Z020 (TRPA1_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transient receptor potential cation channel subfamily A member 1
Short name=dTRPA1

Alternative name(s):
Ankyrin-like with transmembrane domains protein 1
Short name=dANKTM1

Gene names

Name:	TrpA1
Synonyms:	Anktm1
ORF Names:	CG5751

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1296 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Essential for thermotaxis by sensing environmental temperature. Receptor-activated non-selective cation channel involved in detection of sensations such as temperature. Involved in heat nociception by being activated by warm temperature of about 24-29 degrees Celsius. Ref.3 Ref.4
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Disruption phenotype	Eliminates avoidance of elevated temperatures along a thermal gradient. Ref.4
Sequence similarities	Belongs to the transient receptor (TC 1.A.4) family. [View classification] Contains 14 ANK repeats.

Ontologies

Keywords
Biological process	Ion transport Sensory transduction Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	ANK repeat Repeat Transmembrane Transmembrane helix
Molecular function	Ionic channel
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular response to heat Inferred from direct assay. Source: FlyBase circadian sleep/wake cycle Inferred from mutant phenotype. Source: FlyBase detection of chemical stimulus involved in sensory perception of bitter taste Inferred from mutant phenotype. Source: FlyBase memory Inferred from mutant phenotype. Source: FlyBase thermotaxis Inferred from direct assay. Source: FlyBase
Cellular component	integral to membrane Inferred by curator Ref.3. Source: UniProtKB
Molecular function	cation channel activity Inferred from direct assay Ref.3. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform E (identifier: Q7Z020-3)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform F (identifier: Q7Z020-2)

The sequence of this isoform differs from the canonical sequence as follows:
     789-819: AFLQCPFMFAKIDEKTGESITTASPIPLPAL → WPYQKTPEQIEAKRKEFNDPKWRPAPLAVV

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1296	1296	Transient receptor potential cation channel subfamily A member 1		PRO_0000215373
Regions
	Topological domain	1 – 852	852	Cytoplasmic Potential
	Transmembrane	853 – 873	21	Helical; Name=1; Potential
	Topological domain	874 – 926	53	Extracellular Potential
	Transmembrane	927 – 947	21	Helical; Name=2; Potential
	Topological domain	948 – 955	8	Cytoplasmic Potential
	Transmembrane	956 – 976	21	Helical; Name=3; Potential
	Topological domain	977 – 988	12	Extracellular Potential
	Transmembrane	989 – 1009	21	Helical; Name=4; Potential
	Topological domain	1010 – 1031	22	Cytoplasmic Potential
	Transmembrane	1032 – 1052	21	Helical; Name=5; Potential
	Topological domain	1053 – 1070	18	Extracellular Potential
	Transmembrane	1071 – 1093	23	Helical; Name=6; Potential
	Topological domain	1094 – 1103	10	Cytoplasmic Potential
	Transmembrane	1104 – 1124	21	Helical; Name=7; Potential
	Topological domain	1125 – 1296	172	Extracellular Potential
	Repeat	188 – 217	30	ANK 1
	Repeat	221 – 250	30	ANK 2
	Repeat	254 – 283	30	ANK 3
	Repeat	289 – 318	30	ANK 4
	Repeat	323 – 352	30	ANK 5
	Repeat	364 – 393	30	ANK 6
	Repeat	397 – 426	30	ANK 7
	Repeat	435 – 464	30	ANK 8
	Repeat	468 – 497	30	ANK 9
	Repeat	542 – 571	30	ANK 10
	Repeat	575 – 604	30	ANK 11
	Repeat	611 – 640	30	ANK 12
	Repeat	643 – 673	31	ANK 13
	Repeat	677 – 706	30	ANK 14
Amino acid modifications
	Glycosylation	884	1	N-linked (GlcNAc...) Potential
	Glycosylation	901	1	N-linked (GlcNAc...) Potential
	Glycosylation	922	1	N-linked (GlcNAc...) Potential
	Glycosylation	1174	1	N-linked (GlcNAc...) Potential
	Glycosylation	1293	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	789 – 819	31	AFLQC…PLPAL → WPYQKTPEQIEAKRKEFNDP KWRPAPLAVV in isoform F.		VSP_030263

Sequences

	Sequence		Length	Mass (Da)
	Isoform E [UniParc]. Last modified May 26, 2009. Version 3. Checksum: F7A213B00F2E274E	FASTA	1,296	145,971
	10 20 30 40 50 60 MPKLYNGVYS GQCGALSPPD LMEAQPKLLP KPRSNSSGST GRNSKYWIFS MIIERSAGPK 70 80 90 100 110 120 RIEIDGDDAD TPLEAILPAE PPAEVCLLRD SPFRILRSTM TSGDKETPKR EDFASALRFL 130 140 150 160 170 180 MGGCAREPEM TAMAPLNLPK KWARILRMSS TPKIPIVDYL EAAESGNLDD FKRLFMADNS 190 200 210 220 230 240 RIALKDAKGR TAAHQAAARN RVNILRYIRD QNGDFNAKDN AGNTPLHIAV ESDAYDALDY 250 260 270 280 290 300 LLSIPVDTGV LNEKKQAPVH LATELNKVKS LRVMGQYRNV IDIQQGGEHG RTALHLAAIY 310 320 330 340 350 360 DHEECARILI TEFDACPRKP CNNGYYPIHE AAKNASSKTM EVFFQWGEQR GCTREEMISF 370 380 390 400 410 420 YDSEGNVPLH SAVHGGDIKA VELCLKSGAK ISTQQHDLST PVHLACAQGA IDIVKLMFEM 430 440 450 460 470 480 QPMEKRLCLS CTDVQKMTPL HCASMFDHPD IVSYLVAEGA DINALDKEHR SPLLLAASRS 490 500 510 520 530 540 GWKTVHLLIR LGACISVKDA AARNVLHFVI MNGGRLTDFA EQVANCQTQA QLKLLLNEKD 550 560 570 580 590 600 SMGCSPLHYA SRDGHIRSLE NLIRLGACIN LKNNNNESPL HFAARYGRYN TVRQLLDSEK 610 620 630 640 650 660 GSFIINESDG AGMTPLHISS QQGHTRVVQL LLNRGALLHR DHTGRNPLQL AAMSGYTETI 670 680 690 700 710 720 ELLHSVHSHL LDQVDKDGNT ALHLATMENK PHAISVLMSM GCKLVYNVLD MSAIDYAIYY 730 740 750 760 770 780 KYPEAALAMV THEERANEVM ALRSDKHPCV TLALIASMPK VFEAVQDKCI TKANCKKDSK 790 800 810 820 830 840 SFYIKYSFAF LQCPFMFAKI DEKTGESITT ASPIPLPALN TMVTHGRVEL LAHPLSQKYL 850 860 870 880 890 900 QMKWNSYGKY FHLANLLIYS IFLVFVTIYS SLMMNNIELK AGDNKTMSQY CNMGWEQLTM 910 920 930 940 950 960 NLSQNPSVAS QIRLDSCEER INRTTAILFC AVVIVVYILL NSMRELIQIY QQKLHYILET 970 980 990 1000 1010 1020 VNLISWVLYI SALVMVTPAF QPDGGINTIH YSAASIAVFL SWFRLLLFLQ RFDQVGIYVV 1030 1040 1050 1060 1070 1080 MFLEILQTLI KVLMVFSILI IAFGLAFYIL LSKIIDPQPN HLSFSNIPMS LLRTFSMMLG 1090 1100 1110 1120 1130 1140 ELDFVGTYVN TYYRDQLKVP MTSFLILSVF MILMPILLMN LLIGLAVGDI ESVRRNAQLK 1150 1160 1170 1180 1190 1200 RLAMQVVLHT ELERKLPHVW LQRVDKMELI EYPNETKCKL GFCDFILRKW FSNPFTEDSS 1210 1220 1230 1240 1250 1260 MDVISFDNND DYINAELERQ RRKLRDISRM LEQQHHLVRL IVQKMEIKTE ADDVDEGISP 1270 1280 1290 NELRSVVGLR SAGGNRWNSP RVRNKLRAAL SFNKSM
	Isoform F [UniParc]. Checksum: 01333DE406A37216	FASTA	1,295	146,256
	10 20 30 40 50 60 MPKLYNGVYS GQCGALSPPD LMEAQPKLLP KPRSNSSGST GRNSKYWIFS MIIERSAGPK 70 80 90 100 110 120 RIEIDGDDAD TPLEAILPAE PPAEVCLLRD SPFRILRSTM TSGDKETPKR EDFASALRFL 130 140 150 160 170 180 MGGCAREPEM TAMAPLNLPK KWARILRMSS TPKIPIVDYL EAAESGNLDD FKRLFMADNS 190 200 210 220 230 240 RIALKDAKGR TAAHQAAARN RVNILRYIRD QNGDFNAKDN AGNTPLHIAV ESDAYDALDY 250 260 270 280 290 300 LLSIPVDTGV LNEKKQAPVH LATELNKVKS LRVMGQYRNV IDIQQGGEHG RTALHLAAIY 310 320 330 340 350 360 DHEECARILI TEFDACPRKP CNNGYYPIHE AAKNASSKTM EVFFQWGEQR GCTREEMISF 370 380 390 400 410 420 YDSEGNVPLH SAVHGGDIKA VELCLKSGAK ISTQQHDLST PVHLACAQGA IDIVKLMFEM 430 440 450 460 470 480 QPMEKRLCLS CTDVQKMTPL HCASMFDHPD IVSYLVAEGA DINALDKEHR SPLLLAASRS 490 500 510 520 530 540 GWKTVHLLIR LGACISVKDA AARNVLHFVI MNGGRLTDFA EQVANCQTQA QLKLLLNEKD 550 560 570 580 590 600 SMGCSPLHYA SRDGHIRSLE NLIRLGACIN LKNNNNESPL HFAARYGRYN TVRQLLDSEK 610 620 630 640 650 660 GSFIINESDG AGMTPLHISS QQGHTRVVQL LLNRGALLHR DHTGRNPLQL AAMSGYTETI 670 680 690 700 710 720 ELLHSVHSHL LDQVDKDGNT ALHLATMENK PHAISVLMSM GCKLVYNVLD MSAIDYAIYY 730 740 750 760 770 780 KYPEAALAMV THEERANEVM ALRSDKHPCV TLALIASMPK VFEAVQDKCI TKANCKKDSK 790 800 810 820 830 840 SFYIKYSFWP YQKTPEQIEA KRKEFNDPKW RPAPLAVVNT MVTHGRVELL AHPLSQKYLQ 850 860 870 880 890 900 MKWNSYGKYF HLANLLIYSI FLVFVTIYSS LMMNNIELKA GDNKTMSQYC NMGWEQLTMN 910 920 930 940 950 960 LSQNPSVASQ IRLDSCEERI NRTTAILFCA VVIVVYILLN SMRELIQIYQ QKLHYILETV 970 980 990 1000 1010 1020 NLISWVLYIS ALVMVTPAFQ PDGGINTIHY SAASIAVFLS WFRLLLFLQR FDQVGIYVVM 1030 1040 1050 1060 1070 1080 FLEILQTLIK VLMVFSILII AFGLAFYILL SKIIDPQPNH LSFSNIPMSL LRTFSMMLGE 1090 1100 1110 1120 1130 1140 LDFVGTYVNT YYRDQLKVPM TSFLILSVFM ILMPILLMNL LIGLAVGDIE SVRRNAQLKR 1150 1160 1170 1180 1190 1200 LAMQVVLHTE LERKLPHVWL QRVDKMELIE YPNETKCKLG FCDFILRKWF SNPFTEDSSM 1210 1220 1230 1240 1250 1260 DVISFDNNDD YINAELERQR RKLRDISRML EQQHHLVRLI VQKMEIKTEA DDVDEGISPN 1270 1280 1290 ELRSVVGLRS AGGNRWNSPR VRNKLRAALS FNKSM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[3]	"Ion channels: opposite thermosensor in fruitfly and mouse." Viswanath V., Story G.M., Peier A.M., Petrus M.J., Lee V.M., Hwang S.W., Patapoutian A., Jegla T. Nature 423:822-823(2003) [PubMed: 12815418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 100-1296 (ISOFORM E), FUNCTION.
[4]	"The Drosophila ortholog of vertebrate TRPA1 regulates thermotaxis." Rosenzweig M., Brennan K.M., Tayler T.D., Phelps P.O., Patapoutian A., Garrity P.A. Genes Dev. 19:419-424(2005) [PubMed: 15681611] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

The power behind pain - Issue 82 of May 2007

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014296 Genomic DNA. Translation: AAF50356.4. AE014296 Genomic DNA. Translation: ABW08500.3. AY302598 mRNA. Translation: AAP76197.1.
RefSeq	NP_001097554.3. NM_001104084.3. NP_648263.4. NM_140006.4.
UniGene	Dm.15461.
3D structure databases
ProteinModelPortal	Q7Z020.
SMR	Q7Z020. Positions 48-768.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7Z020.
Protein family/group databases
TCDB	1.A.4.6.2. transient receptor potential Ca2+ channel (TRP-CC) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0300208; FBpp0289445; FBgn0035934.
GeneID	39015.
KEGG	dme:Dmel_CG5751.
Organism-specific databases
CTD	8989.
FlyBase	FBgn0035934. TrpA1.
Phylogenomic databases
eggNOG	inNOG08973.
GeneTree	EMGT00070000025711.
InParanoid	Q7Z020.
OrthoDB	EOG415DV7.
PhylomeDB	Q7Z020.
Gene expression databases
Bgee	Q7Z020.
Family and domain databases
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR005821. Ion_trans. [Graphical view]
Gene3D	G3DSA:1.25.40.20. ANK. 4 hits.
KO	K04984.
Pfam	PF12796. Ank_2. 5 hits. PF00520. Ion_trans. 1 hit. [Graphical view]
PRINTS	PR01415. ANKYRIN.
SMART	SM00248. ANK. 15 hits. [Graphical view]
SUPFAM	SSF48403. ANK. 3 hits.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 10 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	811470.

Entry information

Entry name

TRPA1_DROME

Accession

Primary (citable) accession number: Q7Z020
Secondary accession number(s): A8JNP0, Q9VSQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	May 26, 2009
Last modified:	January 25, 2012
This is version 67 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project