Reviewed, UniProtKB/Swiss-Prot Q7Z020 (TRPA1_DROME)

Names and origin

Protein names

Recommended name:
    Transient receptor potential cation channel subfamily A member 1
      Short name=dTRPA1
Alternative name(s):
    Ankyrin-like with transmembrane domains protein 1
      Short name=dANKTM1

Gene names

Name:	TrpA1
Synonyms:	Anktm1
ORF Names:	CG5751

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1296 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Essential for thermotaxis by sensing environmental temperature. Receptor-activated non-selective cation channel involved in detection of sensations such as temperature. Involved in heat nociception by being activated by warm temperature of about 24-29 degrees Celsius. Ref.3 Ref.4
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Disruption phenotype	Eliminates avoidance of elevated temperatures along a thermal gradient. Ref.4
Sequence similarities	Belongs to the transient receptor family. Contains 14 ANK repeats.
Sequence caution	The sequence AAF50356.3 differs from that shown. Reason: Erroneous gene model prediction. The sequence ABW08500.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Ion transport Sensory transduction Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	ANK repeat Repeat Transmembrane
Molecular function	Ionic channel
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cation transport Ref.3 Inferred from direct assay. Source: UniProtKB cellular response to heat Inferred from direct assay. Source: FlyBase thermotaxis Ref.4 Inferred from mutant phenotype. Source: FlyBase transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Ref.3 Inferred by curator. Source: UniProtKB
Molecular function	cation channel activity Ref.3 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform E (identifier: Q7Z020-3)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform F (identifier: Q7Z020-2)

The sequence of this isoform differs from the canonical sequence as follows:
     789-819: AFLQCPFMFAKIDEKTGESITTASPIPLPAL → WPYQKTPEQIEAKRKEFNDPKWRPAPLAVV

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1296	1296	Transient receptor potential cation channel subfamily A member 1		PRO_0000215373
Regions
	Topological domain	1 – 852	852	Cytoplasmic Potential
	Transmembrane	853 – 873	21	1 Potential
	Topological domain	874 – 926	53	Extracellular Potential
	Transmembrane	927 – 947	21	2 Potential
	Topological domain	948 – 955	8	Cytoplasmic Potential
	Transmembrane	956 – 976	21	3 Potential
	Topological domain	977 – 988	12	Extracellular Potential
	Transmembrane	989 – 1009	21	4 Potential
	Topological domain	1010 – 1031	22	Cytoplasmic Potential
	Transmembrane	1032 – 1052	21	5 Potential
	Topological domain	1053 – 1070	18	Extracellular Potential
	Transmembrane	1071 – 1093	23	6 Potential
	Topological domain	1094 – 1103	10	Cytoplasmic Potential
	Transmembrane	1104 – 1124	21	7 Potential
	Topological domain	1125 – 1296	172	Extracellular Potential
	Repeat	188 – 217	30	ANK 1
	Repeat	221 – 250	30	ANK 2
	Repeat	254 – 283	30	ANK 3
	Repeat	289 – 318	30	ANK 4
	Repeat	323 – 352	30	ANK 5
	Repeat	364 – 393	30	ANK 6
	Repeat	397 – 426	30	ANK 7
	Repeat	435 – 464	30	ANK 8
	Repeat	468 – 497	30	ANK 9
	Repeat	542 – 571	30	ANK 10
	Repeat	575 – 604	30	ANK 11
	Repeat	611 – 640	30	ANK 12
	Repeat	643 – 673	31	ANK 13
	Repeat	677 – 706	30	ANK 14
Amino acid modifications
	Glycosylation	884	1	N-linked (GlcNAc...) Potential
	Glycosylation	901	1	N-linked (GlcNAc...) Potential
	Glycosylation	922	1	N-linked (GlcNAc...) Potential
	Glycosylation	1174	1	N-linked (GlcNAc...) Potential
	Glycosylation	1293	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	789 – 819	31	AFLQC…PLPAL → WPYQKTPEQIEAKRKEFNDP KWRPAPLAVV in isoform F.		VSP_030263

Sequences

	Sequence		Length	Mass (Da)
	Isoform E [UniParc]. Last modified May 26, 2009. Version 3. Checksum: F7A213B00F2E274E	FASTA	1,296	145,971
	10 20 30 40 50 60 MPKLYNGVYS GQCGALSPPD LMEAQPKLLP KPRSNSSGST GRNSKYWIFS MIIERSAGPK 70 80 90 100 110 120 RIEIDGDDAD TPLEAILPAE PPAEVCLLRD SPFRILRSTM TSGDKETPKR EDFASALRFL 130 140 150 160 170 180 MGGCAREPEM TAMAPLNLPK KWARILRMSS TPKIPIVDYL EAAESGNLDD FKRLFMADNS 190 200 210 220 230 240 RIALKDAKGR TAAHQAAARN RVNILRYIRD QNGDFNAKDN AGNTPLHIAV ESDAYDALDY 250 260 270 280 290 300 LLSIPVDTGV LNEKKQAPVH LATELNKVKS LRVMGQYRNV IDIQQGGEHG RTALHLAAIY 310 320 330 340 350 360 DHEECARILI TEFDACPRKP CNNGYYPIHE AAKNASSKTM EVFFQWGEQR GCTREEMISF 370 380 390 400 410 420 YDSEGNVPLH SAVHGGDIKA VELCLKSGAK ISTQQHDLST PVHLACAQGA IDIVKLMFEM 430 440 450 460 470 480 QPMEKRLCLS CTDVQKMTPL HCASMFDHPD IVSYLVAEGA DINALDKEHR SPLLLAASRS 490 500 510 520 530 540 GWKTVHLLIR LGACISVKDA AARNVLHFVI MNGGRLTDFA EQVANCQTQA QLKLLLNEKD 550 560 570 580 590 600 SMGCSPLHYA SRDGHIRSLE NLIRLGACIN LKNNNNESPL HFAARYGRYN TVRQLLDSEK 610 620 630 640 650 660 GSFIINESDG AGMTPLHISS QQGHTRVVQL LLNRGALLHR DHTGRNPLQL AAMSGYTETI 670 680 690 700 710 720 ELLHSVHSHL LDQVDKDGNT ALHLATMENK PHAISVLMSM GCKLVYNVLD MSAIDYAIYY 730 740 750 760 770 780 KYPEAALAMV THEERANEVM ALRSDKHPCV TLALIASMPK VFEAVQDKCI TKANCKKDSK 790 800 810 820 830 840 SFYIKYSFAF LQCPFMFAKI DEKTGESITT ASPIPLPALN TMVTHGRVEL LAHPLSQKYL 850 860 870 880 890 900 QMKWNSYGKY FHLANLLIYS IFLVFVTIYS SLMMNNIELK AGDNKTMSQY CNMGWEQLTM 910 920 930 940 950 960 NLSQNPSVAS QIRLDSCEER INRTTAILFC AVVIVVYILL NSMRELIQIY QQKLHYILET 970 980 990 1000 1010 1020 VNLISWVLYI SALVMVTPAF QPDGGINTIH YSAASIAVFL SWFRLLLFLQ RFDQVGIYVV 1030 1040 1050 1060 1070 1080 MFLEILQTLI KVLMVFSILI IAFGLAFYIL LSKIIDPQPN HLSFSNIPMS LLRTFSMMLG 1090 1100 1110 1120 1130 1140 ELDFVGTYVN TYYRDQLKVP MTSFLILSVF MILMPILLMN LLIGLAVGDI ESVRRNAQLK 1150 1160 1170 1180 1190 1200 RLAMQVVLHT ELERKLPHVW LQRVDKMELI EYPNETKCKL GFCDFILRKW FSNPFTEDSS 1210 1220 1230 1240 1250 1260 MDVISFDNND DYINAELERQ RRKLRDISRM LEQQHHLVRL IVQKMEIKTE ADDVDEGISP 1270 1280 1290 NELRSVVGLR SAGGNRWNSP RVRNKLRAAL SFNKSM
	Isoform F. Checksum: 01333DE406A37216	FASTA	1,295	146,256
	10 20 30 40 50 60 MPKLYNGVYS GQCGALSPPD LMEAQPKLLP KPRSNSSGST GRNSKYWIFS MIIERSAGPK 70 80 90 100 110 120 RIEIDGDDAD TPLEAILPAE PPAEVCLLRD SPFRILRSTM TSGDKETPKR EDFASALRFL 130 140 150 160 170 180 MGGCAREPEM TAMAPLNLPK KWARILRMSS TPKIPIVDYL EAAESGNLDD FKRLFMADNS 190 200 210 220 230 240 RIALKDAKGR TAAHQAAARN RVNILRYIRD QNGDFNAKDN AGNTPLHIAV ESDAYDALDY 250 260 270 280 290 300 LLSIPVDTGV LNEKKQAPVH LATELNKVKS LRVMGQYRNV IDIQQGGEHG RTALHLAAIY 310 320 330 340 350 360 DHEECARILI TEFDACPRKP CNNGYYPIHE AAKNASSKTM EVFFQWGEQR GCTREEMISF 370 380 390 400 410 420 YDSEGNVPLH SAVHGGDIKA VELCLKSGAK ISTQQHDLST PVHLACAQGA IDIVKLMFEM 430 440 450 460 470 480 QPMEKRLCLS CTDVQKMTPL HCASMFDHPD IVSYLVAEGA DINALDKEHR SPLLLAASRS 490 500 510 520 530 540 GWKTVHLLIR LGACISVKDA AARNVLHFVI MNGGRLTDFA EQVANCQTQA QLKLLLNEKD 550 560 570 580 590 600 SMGCSPLHYA SRDGHIRSLE NLIRLGACIN LKNNNNESPL HFAARYGRYN TVRQLLDSEK 610 620 630 640 650 660 GSFIINESDG AGMTPLHISS QQGHTRVVQL LLNRGALLHR DHTGRNPLQL AAMSGYTETI 670 680 690 700 710 720 ELLHSVHSHL LDQVDKDGNT ALHLATMENK PHAISVLMSM GCKLVYNVLD MSAIDYAIYY 730 740 750 760 770 780 KYPEAALAMV THEERANEVM ALRSDKHPCV TLALIASMPK VFEAVQDKCI TKANCKKDSK 790 800 810 820 830 840 SFYIKYSFWP YQKTPEQIEA KRKEFNDPKW RPAPLAVVNT MVTHGRVELL AHPLSQKYLQ 850 860 870 880 890 900 MKWNSYGKYF HLANLLIYSI FLVFVTIYSS LMMNNIELKA GDNKTMSQYC NMGWEQLTMN 910 920 930 940 950 960 LSQNPSVASQ IRLDSCEERI NRTTAILFCA VVIVVYILLN SMRELIQIYQ QKLHYILETV 970 980 990 1000 1010 1020 NLISWVLYIS ALVMVTPAFQ PDGGINTIHY SAASIAVFLS WFRLLLFLQR FDQVGIYVVM 1030 1040 1050 1060 1070 1080 FLEILQTLIK VLMVFSILII AFGLAFYILL SKIIDPQPNH LSFSNIPMSL LRTFSMMLGE 1090 1100 1110 1120 1130 1140 LDFVGTYVNT YYRDQLKVPM TSFLILSVFM ILMPILLMNL LIGLAVGDIE SVRRNAQLKR 1150 1160 1170 1180 1190 1200 LAMQVVLHTE LERKLPHVWL QRVDKMELIE YPNETKCKLG FCDFILRKWF SNPFTEDSSM 1210 1220 1230 1240 1250 1260 DVISFDNNDD YINAELERQR RKLRDISRML EQQHHLVRLI VQKMEIKTEA DDVDEGISPN 1270 1280 1290 ELRSVVGLRS AGGNRWNSPR VRNKLRAALS FNKSM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[3]	"Ion channels: opposite thermosensor in fruitfly and mouse." Viswanath V., Story G.M., Peier A.M., Petrus M.J., Lee V.M., Hwang S.W., Patapoutian A., Jegla T. Nature 423:822-823(2003) [PubMed: 12815418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 100-1296 (ISOFORM E), FUNCTION.
[4]	"The Drosophila ortholog of vertebrate TRPA1 regulates thermotaxis." Rosenzweig M., Brennan K.M., Tayler T.D., Phelps P.O., Patapoutian A., Garrity P.A. Genes Dev. 19:419-424(2005) [PubMed: 15681611] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

The power behind pain - Issue 82 of May 2007

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014296 Genomic DNA. Translation: AAF50356.3. Sequence problems. AE014296 Genomic DNA. Translation: ABW08500.2. Sequence problems. AY302598 mRNA. Translation: AAP76197.1.
RefSeq	NP_001097554.2. NP_648263.3.
UniGene	Dm.15461
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q7Z020.
Protein family/group databases
TCDB	1.A.4.6.2. transient receptor potential Ca2+ channel (TRP-CC) family.
Genome annotation databases
Ensembl	FBtr0300208; FBpp0289445; FBgn0035934; Drosophila melanogaster. [Genome view] FBtr0300209; FBpp0289446; FBgn0035934; Drosophila melanogaster. [Genome view]
GeneID	39015.
KEGG	dme:Dmel_CG5751.
Organism-specific databases
CTD	39015.
FlyBase	FBgn0035934. TrpA1.
Phylogenomic databases
OrthoDB	EOG9RXZ62
Gene expression databases
Bgee	Q7Z020.
Family and domain databases
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR005821. Ion_trans. [Graphical view]
Gene3D	G3DSA:1.25.40.20. ANK. 3 hits.
Pfam	PF00023. Ank. 13 hits. PF00520. Ion_trans. 1 hit. [Graphical view]
SMART	SM00248. ANK. 15 hits. [Graphical view]
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 10 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	811470.

Entry information

Entry name

TRPA1_DROME

Accession

Primary (citable) accession number: Q7Z020
Secondary accession number(s): A8JNP0, Q9VSQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	May 26, 2009
Last modified:	November 24, 2009
This is version 48 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project