ID   Q7YZB1_CIOIN            Unreviewed;       277 AA.
AC   Q7YZB1;
DT   01-OCT-2003, integrated into UniProtKB/TrEMBL.
DT   01-OCT-2003, sequence version 1.
DT   27-MAR-2024, entry version 60.
DE   RecName: Full=Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase {ECO:0000256|ARBA:ARBA00012641, ECO:0000256|RuleBase:RU363127};
DE            EC=2.4.1.135 {ECO:0000256|ARBA:ARBA00012641, ECO:0000256|RuleBase:RU363127};
DE   Flags: Fragment;
GN   Name=uglcat3 {ECO:0000313|EMBL:CAE12169.1};
OS   Ciona intestinalis (Transparent sea squirt) (Ascidia intestinalis).
OC   Eukaryota; Metazoa; Chordata; Tunicata; Ascidiacea; Phlebobranchia;
OC   Cionidae; Ciona.
OX   NCBI_TaxID=7719 {ECO:0000313|EMBL:CAE12169.1};
RN   [1] {ECO:0000313|EMBL:CAE12169.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Martinez-Duncker I., Mollicone R., Candelier J.J., Codogno P., Oriol R.;
RT   "Phylogeny of beta3-glucuronyltransferases.";
RL   Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-O-(beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-
CC         xylosyl)-L-seryl-[protein] + UDP-alpha-D-glucuronate = 3-O-(beta-D-
CC         GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-seryl-
CC         [protein] + H(+) + UDP; Xref=Rhea:RHEA:24168, Rhea:RHEA-COMP:12571,
CC         Rhea:RHEA-COMP:12573, ChEBI:CHEBI:15378, ChEBI:CHEBI:58052,
CC         ChEBI:CHEBI:58223, ChEBI:CHEBI:132090, ChEBI:CHEBI:132093;
CC         EC=2.4.1.135; Evidence={ECO:0000256|ARBA:ARBA00000010,
CC         ECO:0000256|RuleBase:RU363127};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000256|PIRSR:PIRSR605027-3,
CC         ECO:0000256|RuleBase:RU363127};
CC   -!- PATHWAY: Protein modification; protein glycosylation.
CC       {ECO:0000256|RuleBase:RU363127}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC       {ECO:0000256|RuleBase:RU363127}; Single-pass type II membrane protein
CC       {ECO:0000256|RuleBase:RU363127}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 43 family.
CC       {ECO:0000256|ARBA:ARBA00007706, ECO:0000256|RuleBase:RU363127}.
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DR   EMBL; AJ577582; CAE12169.1; -; mRNA.
DR   AlphaFoldDB; Q7YZB1; -.
DR   CAZy; GT43; Glycosyltransferase Family 43.
DR   UniPathway; UPA00378; -.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015018; F:galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006486; P:protein glycosylation; IEA:UniProtKB-UniPathway.
DR   CDD; cd00218; GlcAT-I; 1.
DR   InterPro; IPR005027; Glyco_trans_43.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   PANTHER; PTHR10896; GALACTOSYLGALACTOSYLXYLOSYLPROTEIN 3-BETA-GLUCURONOSYLTRANSFERASE BETA-1,3-GLUCURONYLTRANSFERASE; 1.
DR   PANTHER; PTHR10896:SF72; GALACTOSYLGALACTOSYLXYLOSYLPROTEIN 3-BETA-GLUCURONOSYLTRANSFERASE I; 1.
DR   Pfam; PF03360; Glyco_transf_43; 1.
DR   SUPFAM; SSF53448; Nucleotide-diphospho-sugar transferases; 1.
PE   2: Evidence at transcript level;
KW   Golgi apparatus {ECO:0000256|RuleBase:RU363127};
KW   Manganese {ECO:0000256|PIRSR:PIRSR605027-3, ECO:0000256|RuleBase:RU363127};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR605027-3,
KW   ECO:0000256|RuleBase:RU363127};
KW   Signal-anchor {ECO:0000256|RuleBase:RU363127};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU363127};
KW   Transmembrane {ECO:0000256|RuleBase:RU363127}.
FT   ACT_SITE        241
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR605027-1"
FT   BINDING         158
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR605027-3"
FT   SITE            189
FT                   /note="Interaction with galactose moiety of substrate
FT                   glycoprotein"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR605027-4"
FT   NON_TER         1
FT                   /evidence="ECO:0000313|EMBL:CAE12169.1"
SQ   SEQUENCE   277 AA;  32181 MW;  C5922AE5BB90EE15 CRC64;
     RHEGCLLIMA IIDIALLAIN FCYSRDFLTI RPQKAKAFCK TKPSFQVETI KENMPIIYGV
     TSTYKRYLQL PELTRLSQTL LHIPKFHWIL TEDSYQKTKT VTKFLQKSGL NYTHLNTKNN
     IPPTKFIKDY NTRRMGLNWI RENVPPTKDA IVYFIDDDNT YSLKLFDEIR ATKRAAVWQV
     GLVGGILNEG PVKCENGKVL EWKAYWWPDR LIPIDMAGFA VHVKLLFERP KAEFCDLPDM
     ESDFLSSLCV TRDNIEANNC NDVLVWHTQT KTPEVEE
//