Q7YT16 (LYS1_MUSDO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Lysozyme 1 EC=3.2.1.17 Alternative name(s): 1,4-beta-N-acetylmuramidase 1
Organism	Musca domestica (House fly)
Taxonomic identifier	7370 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Muscoidea › Muscidae › Musca

Protein attributes

Sequence length	141 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May not function as a self-defense protein, but as a digestive enzyme, probably in the gut of the insect body. Inactive towards Micrococcus luteus. Active toward glycol chitin.
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Sequence similarities	Belongs to the glycosyl hydrolase 22 family.
Biophysicochemical properties	pH dependence: Active at acidic pHs. Inactive above pH 7.

Ontologies

Keywords
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Ref.2

Chain

20 – 141

122

Lysozyme 1

PRO_0000018508

Sites

Active site

51

1

By similarity

Active site

69

1

By similarity

Amino acid modifications

Glycosylation

65

1

N-linked (GlcNAc...) Potential

Glycosylation

104

1

N-linked (GlcNAc...) Potential

Disulfide bond

25 ↔ 140

By similarity

Disulfide bond

46 ↔ 130

By similarity

Disulfide bond

81 ↔ 97

By similarity

Disulfide bond

93 ↔ 111

By similarity

Secondary structure

1

.

.

.

.

.

.

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.

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.

.

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141

Helix Strand Turn

Details...

Helix

24 – 33

10

Helix

38 – 40

3

Helix

41 – 52

12

Beta strand

55 – 57

3

Turn

71 – 74

4

Turn

77 – 80

4

Helix

97 – 101

5

Beta strand

102 – 104

3

Helix

106 – 119

14

Helix

121 – 123

3

Helix

127 – 130

4

Helix

138 – 140

3

Sequences

	Sequence		Length	Mass (Da)
	Q7YT16-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: C640C6407AD80F0E	FASTA	141	15,733
	10 20 30 40 50 60 MKFFIVLVAA LALAAPAMGK TFTRCSLARE MYALGVPKSE LPQWTCIAEH ESSYRTNVVG 70 80 90 100 110 120 PTNSNGSNDY GIFQINNYYW CQPSNGRFSY NECHLSCDAL LTDNISNSVT CARKIKSQQG 130 140 WTAWSTWKYC SGSLPSINDC F

References

[1]	"Cloning, sequencing and characterization of a digestive lysozyme from Musca domestica midgut." Chimoy P., Marana S.R., Ferreira C., Terra W.R. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Midgut epithelium.
[2]	"Insect lysozyme from house fly (Musca domestica) larvae: possible digestive function based on sequence and enzymatic properties." Ito Y., Nakamura M., Hotani T., Imoto T. J. Biochem. 118:546-551(1995) [PubMed: 8690715] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-141. Tissue: Larva.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY344589 mRNA. Translation: AAQ20048.1.

PIR

PC4062.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2FBD	X-ray	1.90	A/B	20-141	[»]
2H5Z	X-ray	1.92	A/B	20-141	[»]

ProteinModelPortal

Q7YT16.

ModBase

Search...

Protein family/group databases

CAZy

GH22. Glycoside Hydrolase Family 22.

Enzyme and pathway databases

BRENDA

3.2.1.17. 21508.

Family and domain databases

InterPro

IPR001916. Glyco_hydro_22.
IPR019799. Glyco_hydro_22_CS.
IPR000974. Glyco_hydro_22_lys.
[Graphical view]

Pfam

PF00062. Lys. 1 hit.
[Graphical view]

PRINTS

PR00137. LYSOZYME.
PR00135. LYZLACT.

SMART

SM00263. LYZ1. 1 hit.
[Graphical view]

PROSITE

PS00128. LACTALBUMIN_LYSOZYME_1. 1 hit.
PS51348. LACTALBUMIN_LYSOZYME_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

LYS1_MUSDO

Accession

Primary (citable) accession number: Q7YT16
Secondary accession number(s): Q7M437

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	October 1, 2003
Last modified:	August 10, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)