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Q7YSW8

- PP2BA_DICDI

UniProt

Q7YSW8 - PP2BA_DICDI

Protein

Calcineurin subunit A

Gene

canA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    Catalytic subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase.1 Publication

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.1 Publication

    Cofactori

    Binds 1 Fe3+ ion per subunit.
    Binds 1 zinc ion per subunit.

    Enzyme regulationi

    Activated by arachidonic acid, linoleic acid and oleic acid at the same extent than calmodulin.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi138 – 1381IronBy similarity
    Metal bindingi140 – 1401IronBy similarity
    Metal bindingi168 – 1681IronBy similarity
    Metal bindingi168 – 1681ZincBy similarity
    Metal bindingi200 – 2001ZincBy similarity
    Active sitei201 – 2011Proton donorBy similarity
    Metal bindingi249 – 2491ZincBy similarity
    Metal bindingi330 – 3301ZincBy similarity

    GO - Molecular functioni

    1. calcium ion binding Source: dictyBase
    2. calmodulin binding Source: dictyBase
    3. protein binding Source: dictyBase
    4. protein serine/threonine phosphatase activity Source: dictyBase

    GO - Biological processi

    1. protein dephosphorylation Source: dictyBase
    2. regulation of sorocarp development Source: dictyBase
    3. response to cation stress Source: dictyBase

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Calmodulin-binding, Iron, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_219084. Ca2+ pathway.
    REACT_221868. DARPP-32 events.
    REACT_227520. FCERI mediated Ca+2 mobilization.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calcineurin subunit A (EC:3.1.3.16)
    Alternative name(s):
    Serine/threonine-protein phosphatase 2B catalytic subunit
    Gene namesi
    Name:canA
    ORF Names:DDB_G0276883
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0276883. canA.

    Subcellular locationi

    GO - Cellular componenti

    1. calcineurin complex Source: dictyBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 623623Calcineurin subunit APRO_0000331279Add
    BLAST

    Post-translational modificationi

    The N-terminus is blocked.

    Expressioni

    Developmental stagei

    Highest levels are found after 10 hours of development. Levels decrease during multicellular development (at protein level).1 Publication

    Interactioni

    Subunit structurei

    Calcineurin is composed of a catalytic subunit (A) and a regulatory subunit (B).

    Protein-protein interaction databases

    IntActiQ7YSW8. 1 interaction.
    STRINGi44689.DDB_0185021.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7YSW8.
    SMRiQ7YSW8. Positions 52-421.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni41 – 350310CatalyticBy similarityAdd
    BLAST
    Regioni296 – 3027Calcineurin B binding-site 1Sequence Analysis
    Regioni345 – 3506Calcineurin B binding-site 2Sequence Analysis
    Regioni444 – 46623Calmodulin-bindingSequence AnalysisAdd
    BLAST
    Regioni507 – 55448Inhibitory domainBy similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili422 – 46039Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi596 – 60813Poly-GlnAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-2B subfamily.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG0639.
    KOiK04348.
    OMAiIFENDIG.
    PhylomeDBiQ7YSW8.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7YSW8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTNNNKAPT SQSSDKSATT ESKLNDNENT ETETKTDENN EEKPKMTLKP    50
    PQPKIENKVS TIDRVVPSVK YPKSLPLPHD VLFNEDKTIN LPKLQEHFFA 100
    EGRLNHDDVI EIVKMAAEIL EKEPTLIQVE APITVCGDTH GQFYDLIKIF 150
    ENDIGGNPAN TNYLFLGDYV DRGYFSMEVI IYLYACKINY PNTFFLLRGN 200
    HECRHLTEYF TFKEECLHKY SEEVYDFITE SFNALPLAAL MNGKFLCIHG 250
    GLSPDIKTLD DIANIDRFKE PPSSGPMCDL LWSDPMEEFS PEIREHFVPN 300
    DVRGCSYLYS YRAVCSFLQK NKLLSVIRAH EAQNAGYKMH LQNDATGFPS 350
    VITLFSAPNY LDAYNNKGAV LRYENNVMNI RQFTCSPHPY WLPNFMDVFT 400
    WSMPFVSEKV AEMLLVLLNL CNDEEAEKNE NAQTVKDTSE EEKRRQMLRA 450
    KVKSVSKMMR MFSLLRQERE TIMMIKSFSP SRKIPQGLLT EGKDALKKAL 500
    GDFAQARKMD LINEKRPPIL DRVNSRGELL RMNSRGELFR INSKGDLFRS 550
    NSYADLKPPQ GPQETIKITE CHEQNITTNN INPNSITTNE NNSNEQLQQQ 600
    QQQQQQQQPP TTTSTTTQTE VAK 623
    Length:623
    Mass (Da):71,393
    Last modified:October 1, 2003 - v1
    Checksum:iEB9E46E18795FA6C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti77 – 771L → I in CAA65935. (PubMed:8681950)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X97280 mRNA. Translation: CAA65935.1.
    U22397 mRNA. Translation: AAB82063.1.
    AAFI02000019 Genomic DNA. Translation: EAL68943.1.
    RefSeqiXP_642811.1. XM_637719.1.

    Genome annotation databases

    EnsemblProtistsiDDB0185021; DDB0185021; DDB_G0276883.
    GeneIDi8620674.
    KEGGiddi:DDB_G0276883.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X97280 mRNA. Translation: CAA65935.1 .
    U22397 mRNA. Translation: AAB82063.1 .
    AAFI02000019 Genomic DNA. Translation: EAL68943.1 .
    RefSeqi XP_642811.1. XM_637719.1.

    3D structure databases

    ProteinModelPortali Q7YSW8.
    SMRi Q7YSW8. Positions 52-421.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q7YSW8. 1 interaction.
    STRINGi 44689.DDB_0185021.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0185021 ; DDB0185021 ; DDB_G0276883 .
    GeneIDi 8620674.
    KEGGi ddi:DDB_G0276883.

    Organism-specific databases

    dictyBasei DDB_G0276883. canA.

    Phylogenomic databases

    eggNOGi COG0639.
    KOi K04348.
    OMAi IFENDIG.
    PhylomeDBi Q7YSW8.

    Enzyme and pathway databases

    Reactomei REACT_219084. Ca2+ pathway.
    REACT_221868. DARPP-32 events.
    REACT_227520. FCERI mediated Ca+2 mobilization.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure, expression and developmental regulation of a Dictyostelium calcineurin A homologue."
      Dammann H., Hellstern S., Husain Q., Mutzel R.
      Eur. J. Biochem. 238:391-399(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, DEVELOPMENTAL STAGE.
    2. "Cloning and characterization of calcineurin from Dictyostelium discoideum."
      Lydan M.A., O'Day D.H., Cotter D.A.
      Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: AX3-1.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    4. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    5. "Overexpression, purification and characterization of Dictyostelium calcineurin A."
      Hellstern S., Dammann H., Husain Q., Mutzel R.
      Res. Microbiol. 148:335-343(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Ca(2+)/calmodulin-independent activation of calcineurin from Dictyostelium by unsaturated long chain fatty acids."
      Kessen U., Schaloske R., Aichem A., Mutzel R.
      J. Biol. Chem. 274:37821-37826(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME REGULATION.
    7. "Unconventional mRNA processing in the expression of two calcineurin B isoforms in Dictyostelium."
      Aichem A., Mutzel R.
      J. Mol. Biol. 308:873-882(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CNBA.

    Entry informationi

    Entry nameiPP2BA_DICDI
    AccessioniPrimary (citable) accession number: Q7YSW8
    Secondary accession number(s): Q27558, Q27560, Q550X1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3