Q7YSW8 (PP2BA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calcineurin subunit A
EC=3.1.3.16

Alternative name(s):
Serine/threonine-protein phosphatase 2B catalytic subunit

Gene names

Name:	canA
ORF Names:	DDB_G0276883

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	623 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalytic subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Ref.5
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate. Ref.1
Cofactor	Binds 1 Fe3+ ion per subunit. Binds 1 zinc ion per subunit.
Enzyme regulation	Activated by arachidonic acid, linoleic acid and oleic acid at the same extent than calmodulin. Ref.6
Subunit structure	Calcineurin is composed of a catalytic subunit (A) and a regulatory subunit (B).
Developmental stage	Highest levels are found after 10 hours of development. Levels decrease during multicellular development (at protein level). Ref.1
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the PPP phosphatase family. PP-2B subfamily.

Ontologies

Keywords
Domain	Coiled coil
Ligand	Calmodulin-binding Iron Metal-binding Zinc
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein dephosphorylation Inferred from direct assay Ref.5. Source: dictyBase
Cellular_component	calcineurin complex Inferred by curator Ref.7. Source: dictyBase
Molecular_function	calcium ion binding Inferred from sequence or structural similarity. Source: dictyBase calmodulin binding Inferred from direct assay Ref.1. Source: dictyBase protein serine/threonine phosphatase activity Inferred from direct assay Ref.5. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 623	623	Calcineurin subunit A		PRO_0000331279
Regions
	Region	41 – 350	310	Catalytic By similarity
	Region	296 – 302	7	Calcineurin B binding-site 1 Potential
	Region	345 – 350	6	Calcineurin B binding-site 2 Potential
	Region	444 – 466	23	Calmodulin-binding Potential
	Region	507 – 554	48	Inhibitory domain By similarity
	Coiled coil	422 – 460	39	Potential
	Compositional bias	596 – 608	13	Poly-Gln
Sites
	Active site	201	1	Proton donor By similarity
	Metal binding	138	1	Iron By similarity
	Metal binding	140	1	Iron By similarity
	Metal binding	168	1	Iron By similarity
	Metal binding	168	1	Zinc By similarity
	Metal binding	200	1	Zinc By similarity
	Metal binding	249	1	Zinc By similarity
	Metal binding	330	1	Zinc By similarity
Experimental info
	Sequence conflict	77	1	L → I in CAA65935. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q7YSW8 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: EB9E46E18795FA6C	FASTA	623	71,393
	10 20 30 40 50 60 MSTNNNKAPT SQSSDKSATT ESKLNDNENT ETETKTDENN EEKPKMTLKP PQPKIENKVS 70 80 90 100 110 120 TIDRVVPSVK YPKSLPLPHD VLFNEDKTIN LPKLQEHFFA EGRLNHDDVI EIVKMAAEIL 130 140 150 160 170 180 EKEPTLIQVE APITVCGDTH GQFYDLIKIF ENDIGGNPAN TNYLFLGDYV DRGYFSMEVI 190 200 210 220 230 240 IYLYACKINY PNTFFLLRGN HECRHLTEYF TFKEECLHKY SEEVYDFITE SFNALPLAAL 250 260 270 280 290 300 MNGKFLCIHG GLSPDIKTLD DIANIDRFKE PPSSGPMCDL LWSDPMEEFS PEIREHFVPN 310 320 330 340 350 360 DVRGCSYLYS YRAVCSFLQK NKLLSVIRAH EAQNAGYKMH LQNDATGFPS VITLFSAPNY 370 380 390 400 410 420 LDAYNNKGAV LRYENNVMNI RQFTCSPHPY WLPNFMDVFT WSMPFVSEKV AEMLLVLLNL 430 440 450 460 470 480 CNDEEAEKNE NAQTVKDTSE EEKRRQMLRA KVKSVSKMMR MFSLLRQERE TIMMIKSFSP 490 500 510 520 530 540 SRKIPQGLLT EGKDALKKAL GDFAQARKMD LINEKRPPIL DRVNSRGELL RMNSRGELFR 550 560 570 580 590 600 INSKGDLFRS NSYADLKPPQ GPQETIKITE CHEQNITTNN INPNSITTNE NNSNEQLQQQ 610 620 QQQQQQQQPP TTTSTTTQTE VAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure, expression and developmental regulation of a Dictyostelium calcineurin A homologue." Dammann H., Hellstern S., Husain Q., Mutzel R. Eur. J. Biochem. 238:391-399(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, DEVELOPMENTAL STAGE.
[2]	"Cloning and characterization of calcineurin from Dictyostelium discoideum." Lydan M.A., O'Day D.H., Cotter D.A. Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: AX3-1.
[3]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[4]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[5]	"Overexpression, purification and characterization of Dictyostelium calcineurin A." Hellstern S., Dammann H., Husain Q., Mutzel R. Res. Microbiol. 148:335-343(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Ca(2+)/calmodulin-independent activation of calcineurin from Dictyostelium by unsaturated long chain fatty acids." Kessen U., Schaloske R., Aichem A., Mutzel R. J. Biol. Chem. 274:37821-37826(1999) [PubMed] [Europe PMC] [Abstract] Cited for: ENZYME REGULATION.
[7]	"Unconventional mRNA processing in the expression of two calcineurin B isoforms in Dictyostelium." Aichem A., Mutzel R. J. Mol. Biol. 308:873-882(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CNBA.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X97280 mRNA. Translation: CAA65935.1. U22397 mRNA. Translation: AAB82063.1. AAFI02000019 Genomic DNA. Translation: EAL68943.1.
RefSeq	XP_642811.1. XM_637719.1.
3D structure databases
ProteinModelPortal	Q7YSW8.
SMR	Q7YSW8. Positions 52-421.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q7YSW8. 1 interaction.
STRING	44689.DDB_0185021.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0185021; DDB0185021; DDB_G0276883.
GeneID	8620674.
KEGG	ddi:DDB_G0276883.
Organism-specific databases
dictyBase	DDB_G0276883. canA.
Phylogenomic databases
eggNOG	COG0639.
KO	K04348.
OMA	IFENDIG.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PP2BA_DICDI

Accession

Primary (citable) accession number: Q7YSW8
Secondary accession number(s): Q27558, Q27560, Q550X1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	October 1, 2003
Last modified:	May 29, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)