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Protein

Calcineurin subunit A

Gene

canA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • Fe3+Note: Binds 1 Fe3+ ion per subunit.
  • Zn2+Note: Binds 1 zinc ion per subunit.

Enzyme regulationi

Activated by arachidonic acid, linoleic acid and oleic acid at the same extent than calmodulin.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi138 – 1381IronBy similarity
Metal bindingi140 – 1401IronBy similarity
Metal bindingi168 – 1681IronBy similarity
Metal bindingi168 – 1681ZincBy similarity
Metal bindingi200 – 2001ZincBy similarity
Active sitei201 – 2011Proton donorBy similarity
Metal bindingi249 – 2491ZincBy similarity
Metal bindingi330 – 3301ZincBy similarity

GO - Molecular functioni

  • calmodulin binding Source: dictyBase
  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: dictyBase

GO - Biological processi

  • protein dephosphorylation Source: dictyBase
  • regulation of sorocarp development Source: dictyBase
  • response to cation stress Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Calmodulin-binding, Iron, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_284405. Ca2+ pathway.
REACT_334796. FCERI mediated Ca+2 mobilization.
REACT_344348. DARPP-32 events.
REACT_361109. CLEC7A (Dectin-1) induces NFAT activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcineurin subunit A (EC:3.1.3.16)
Alternative name(s):
Serine/threonine-protein phosphatase 2B catalytic subunit
Gene namesi
Name:canA
ORF Names:DDB_G0276883
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 2, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0276883. canA.

Subcellular locationi

GO - Cellular componenti

  • calcineurin complex Source: dictyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 623623Calcineurin subunit APRO_0000331279Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Expressioni

Developmental stagei

Highest levels are found after 10 hours of development. Levels decrease during multicellular development (at protein level).1 Publication

Interactioni

Subunit structurei

Calcineurin is composed of a catalytic subunit (A) and a regulatory subunit (B).

Protein-protein interaction databases

IntActiQ7YSW8. 1 interaction.
STRINGi44689.DDB0185021.

Structurei

3D structure databases

ProteinModelPortaliQ7YSW8.
SMRiQ7YSW8. Positions 52-421.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni41 – 350310CatalyticBy similarityAdd
BLAST
Regioni296 – 3027Calcineurin B binding-site 1Sequence Analysis
Regioni345 – 3506Calcineurin B binding-site 2Sequence Analysis
Regioni444 – 46623Calmodulin-bindingSequence AnalysisAdd
BLAST
Regioni507 – 55448Inhibitory domainBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili422 – 46039Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi596 – 60813Poly-GlnAdd
BLAST

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-2B subfamily.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG0639.
InParanoidiQ7YSW8.
KOiK04348.
OMAiANTESRD.
PhylomeDBiQ7YSW8.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7YSW8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTNNNKAPT SQSSDKSATT ESKLNDNENT ETETKTDENN EEKPKMTLKP
60 70 80 90 100
PQPKIENKVS TIDRVVPSVK YPKSLPLPHD VLFNEDKTIN LPKLQEHFFA
110 120 130 140 150
EGRLNHDDVI EIVKMAAEIL EKEPTLIQVE APITVCGDTH GQFYDLIKIF
160 170 180 190 200
ENDIGGNPAN TNYLFLGDYV DRGYFSMEVI IYLYACKINY PNTFFLLRGN
210 220 230 240 250
HECRHLTEYF TFKEECLHKY SEEVYDFITE SFNALPLAAL MNGKFLCIHG
260 270 280 290 300
GLSPDIKTLD DIANIDRFKE PPSSGPMCDL LWSDPMEEFS PEIREHFVPN
310 320 330 340 350
DVRGCSYLYS YRAVCSFLQK NKLLSVIRAH EAQNAGYKMH LQNDATGFPS
360 370 380 390 400
VITLFSAPNY LDAYNNKGAV LRYENNVMNI RQFTCSPHPY WLPNFMDVFT
410 420 430 440 450
WSMPFVSEKV AEMLLVLLNL CNDEEAEKNE NAQTVKDTSE EEKRRQMLRA
460 470 480 490 500
KVKSVSKMMR MFSLLRQERE TIMMIKSFSP SRKIPQGLLT EGKDALKKAL
510 520 530 540 550
GDFAQARKMD LINEKRPPIL DRVNSRGELL RMNSRGELFR INSKGDLFRS
560 570 580 590 600
NSYADLKPPQ GPQETIKITE CHEQNITTNN INPNSITTNE NNSNEQLQQQ
610 620
QQQQQQQQPP TTTSTTTQTE VAK
Length:623
Mass (Da):71,393
Last modified:October 1, 2003 - v1
Checksum:iEB9E46E18795FA6C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti77 – 771L → I in CAA65935 (PubMed:8681950).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97280 mRNA. Translation: CAA65935.1.
U22397 mRNA. Translation: AAB82063.1.
AAFI02000019 Genomic DNA. Translation: EAL68943.1.
RefSeqiXP_642811.1. XM_637719.1.

Genome annotation databases

EnsemblProtistsiDDB0185021; DDB0185021; DDB_G0276883.
GeneIDi8620674.
KEGGiddi:DDB_G0276883.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97280 mRNA. Translation: CAA65935.1.
U22397 mRNA. Translation: AAB82063.1.
AAFI02000019 Genomic DNA. Translation: EAL68943.1.
RefSeqiXP_642811.1. XM_637719.1.

3D structure databases

ProteinModelPortaliQ7YSW8.
SMRiQ7YSW8. Positions 52-421.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ7YSW8. 1 interaction.
STRINGi44689.DDB0185021.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0185021; DDB0185021; DDB_G0276883.
GeneIDi8620674.
KEGGiddi:DDB_G0276883.

Organism-specific databases

dictyBaseiDDB_G0276883. canA.

Phylogenomic databases

eggNOGiCOG0639.
InParanoidiQ7YSW8.
KOiK04348.
OMAiANTESRD.
PhylomeDBiQ7YSW8.

Enzyme and pathway databases

ReactomeiREACT_284405. Ca2+ pathway.
REACT_334796. FCERI mediated Ca+2 mobilization.
REACT_344348. DARPP-32 events.
REACT_361109. CLEC7A (Dectin-1) induces NFAT activation.

Miscellaneous databases

PROiQ7YSW8.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure, expression and developmental regulation of a Dictyostelium calcineurin A homologue."
    Dammann H., Hellstern S., Husain Q., Mutzel R.
    Eur. J. Biochem. 238:391-399(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, DEVELOPMENTAL STAGE.
  2. "Cloning and characterization of calcineurin from Dictyostelium discoideum."
    Lydan M.A., O'Day D.H., Cotter D.A.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: AX3-1.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  4. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  5. "Overexpression, purification and characterization of Dictyostelium calcineurin A."
    Hellstern S., Dammann H., Husain Q., Mutzel R.
    Res. Microbiol. 148:335-343(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Ca(2+)/calmodulin-independent activation of calcineurin from Dictyostelium by unsaturated long chain fatty acids."
    Kessen U., Schaloske R., Aichem A., Mutzel R.
    J. Biol. Chem. 274:37821-37826(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.
  7. "Unconventional mRNA processing in the expression of two calcineurin B isoforms in Dictyostelium."
    Aichem A., Mutzel R.
    J. Mol. Biol. 308:873-882(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CNBA.

Entry informationi

Entry nameiPP2BA_DICDI
AccessioniPrimary (citable) accession number: Q7YSW8
Secondary accession number(s): Q27558, Q27560, Q550X1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: October 1, 2003
Last modified: July 22, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.