Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q7YSW8 (PP2BA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calcineurin subunit A

EC=3.1.3.16
Alternative name(s):
Serine/threonine-protein phosphatase 2B catalytic subunit
Gene names
Name:canA
ORF Names:DDB_G0276883
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length623 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalytic subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Ref.5

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate. Ref.1

Cofactor

Binds 1 Fe3+ ion per subunit.

Binds 1 zinc ion per subunit.

Enzyme regulation

Activated by arachidonic acid, linoleic acid and oleic acid at the same extent than calmodulin. Ref.6

Subunit structure

Calcineurin is composed of a catalytic subunit (A) and a regulatory subunit (B).

Developmental stage

Highest levels are found after 10 hours of development. Levels decrease during multicellular development (at protein level). Ref.1

Post-translational modification

The N-terminus is blocked.

Sequence similarities

Belongs to the PPP phosphatase family. PP-2B subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 623623Calcineurin subunit A
PRO_0000331279

Regions

Region41 – 350310Catalytic By similarity
Region296 – 3027Calcineurin B binding-site 1 Potential
Region345 – 3506Calcineurin B binding-site 2 Potential
Region444 – 46623Calmodulin-binding Potential
Region507 – 55448Inhibitory domain By similarity
Coiled coil422 – 46039 Potential
Compositional bias596 – 60813Poly-Gln

Sites

Active site2011Proton donor By similarity
Metal binding1381Iron By similarity
Metal binding1401Iron By similarity
Metal binding1681Iron By similarity
Metal binding1681Zinc By similarity
Metal binding2001Zinc By similarity
Metal binding2491Zinc By similarity
Metal binding3301Zinc By similarity

Experimental info

Sequence conflict771L → I in CAA65935. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q7YSW8 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: EB9E46E18795FA6C

FASTA62371,393
        10         20         30         40         50         60 
MSTNNNKAPT SQSSDKSATT ESKLNDNENT ETETKTDENN EEKPKMTLKP PQPKIENKVS 

        70         80         90        100        110        120 
TIDRVVPSVK YPKSLPLPHD VLFNEDKTIN LPKLQEHFFA EGRLNHDDVI EIVKMAAEIL 

       130        140        150        160        170        180 
EKEPTLIQVE APITVCGDTH GQFYDLIKIF ENDIGGNPAN TNYLFLGDYV DRGYFSMEVI 

       190        200        210        220        230        240 
IYLYACKINY PNTFFLLRGN HECRHLTEYF TFKEECLHKY SEEVYDFITE SFNALPLAAL 

       250        260        270        280        290        300 
MNGKFLCIHG GLSPDIKTLD DIANIDRFKE PPSSGPMCDL LWSDPMEEFS PEIREHFVPN 

       310        320        330        340        350        360 
DVRGCSYLYS YRAVCSFLQK NKLLSVIRAH EAQNAGYKMH LQNDATGFPS VITLFSAPNY 

       370        380        390        400        410        420 
LDAYNNKGAV LRYENNVMNI RQFTCSPHPY WLPNFMDVFT WSMPFVSEKV AEMLLVLLNL 

       430        440        450        460        470        480 
CNDEEAEKNE NAQTVKDTSE EEKRRQMLRA KVKSVSKMMR MFSLLRQERE TIMMIKSFSP 

       490        500        510        520        530        540 
SRKIPQGLLT EGKDALKKAL GDFAQARKMD LINEKRPPIL DRVNSRGELL RMNSRGELFR 

       550        560        570        580        590        600 
INSKGDLFRS NSYADLKPPQ GPQETIKITE CHEQNITTNN INPNSITTNE NNSNEQLQQQ 

       610        620 
QQQQQQQQPP TTTSTTTQTE VAK 

« Hide

References

« Hide 'large scale' references
[1]"Primary structure, expression and developmental regulation of a Dictyostelium calcineurin A homologue."
Dammann H., Hellstern S., Husain Q., Mutzel R.
Eur. J. Biochem. 238:391-399(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, DEVELOPMENTAL STAGE.
[2]"Cloning and characterization of calcineurin from Dictyostelium discoideum."
Lydan M.A., O'Day D.H., Cotter D.A.
Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: AX3-1.
[3]"Sequence and analysis of chromosome 2 of Dictyostelium discoideum."
Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A.
Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[4]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[5]"Overexpression, purification and characterization of Dictyostelium calcineurin A."
Hellstern S., Dammann H., Husain Q., Mutzel R.
Res. Microbiol. 148:335-343(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Ca(2+)/calmodulin-independent activation of calcineurin from Dictyostelium by unsaturated long chain fatty acids."
Kessen U., Schaloske R., Aichem A., Mutzel R.
J. Biol. Chem. 274:37821-37826(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION.
[7]"Unconventional mRNA processing in the expression of two calcineurin B isoforms in Dictyostelium."
Aichem A., Mutzel R.
J. Mol. Biol. 308:873-882(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CNBA.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X97280 mRNA. Translation: CAA65935.1.
U22397 mRNA. Translation: AAB82063.1.
AAFI02000019 Genomic DNA. Translation: EAL68943.1.
RefSeqXP_642811.1. XM_637719.1.

3D structure databases

ProteinModelPortalQ7YSW8.
SMRQ7YSW8. Positions 52-421.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ7YSW8. 1 interaction.
STRING44689.DDB_0185021.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0185021; DDB0185021; DDB_G0276883.
GeneID8620674.
KEGGddi:DDB_G0276883.

Organism-specific databases

dictyBaseDDB_G0276883. canA.

Phylogenomic databases

eggNOGCOG0639.
OMAIFENDIG.
PhylomeDBQ7YSW8.

Family and domain databases

Gene3D3.60.21.10. 1 hit.
InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMSSF56300. SSF56300. 1 hit.
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePP2BA_DICDI
AccessionPrimary (citable) accession number: Q7YSW8
Secondary accession number(s): Q27558, Q27560, Q550X1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: October 1, 2003
Last modified: July 9, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase