Reviewed, UniProtKB/Swiss-Prot Q7YS70 (MECR_BOVIN)

Names and origin

Protein names

Recommended name:
    Trans-2-enoyl-CoA reductase, mitochondrial
    EC=1.3.1.38
Alternative name(s):
    BtNrbf-1
      Short name=NRBF-1

Gene names

Name:	MECR
Synonyms:	NBRF1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the reduction of trans-2-enoyl-CoA to acyl-CoA with chain length from C6 to C16 in an NADPH-dependent manner with preference to medium chain length substrate. May have a role in the mitochondrial synthesis of fatty acids.
Catalytic activity	Acyl-CoA + NADP(+) = trans-2,3-dehydroacyl-CoA + NADPH.
Subunit structure	Homodimer.
Subcellular location	MitochondrionBy similarity.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Ref.1 Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	trans-2-enoyl-CoA reductase (NADPH) activity Ref.1 Inferred from direct assay. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 53	53	Mitochondrion Potential
	Chain	54 – 373	320	Trans-2-enoyl-CoA reductase, mitochondrial		PRO_0000000887

Sequences

	Sequence		Length	Mass (Da)
	Q7YS70-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 7921122C7735A95D	FASTA	373	40,275
	10 20 30 40 50 60 MWVCGALCRT RAPAQLGQRL LPESRRRRPA SASFSASAEP SRVRALVYGH HGDPAKVVEL 70 80 90 100 110 120 KNLELAAVGG SHVHVKMLAA PINPSDINMI QGNYGLLPQL PAVGGNEGVG QVVAVGSGVT 130 140 150 160 170 180 GVKPGDWVIP ANPGLGTWRT EAVFGEEELI TVPSDIPLQS AATLGVNPCT AYRMLVDFER 190 200 210 220 230 240 LRPRDSIIQN ASNSGVGQAV IQIAAARGLR TINVLRDTPD LQKLTDTLKN LGANHVVTEE 250 260 270 280 290 300 ELRKPEMKSF FKDVPQPRLA LNCVGGKSST ELLRHLAPGG TMVTYGGMAK QPVIASVSQL 310 320 330 340 350 360 IFKDLKLRGF WLSQWKKDHS PDQFKELILT LCDLIRRGQL TAPACSEVPL QDYLCALEAS 370 TQPFVSSKQI LTM

References

[1]

"Characterization of 2-enoyl thioester reductase from mammals: an ortholog of Ybr026p/Mrf1'p of the yeast mitochondrial fatty acid synthesis type II." Miinalainen I.J., Chen Z.-J., Torkko J.M., Pirilae P.L., Sormunen R.T., Bergmann U., Qin Y.-M., Hiltunen J.K. J. Biol. Chem. 278:20154-20161(2003) [PubMed: 12654921] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION BY MASS SPECTROMETRY, ENZYME ACTIVITY, SUBUNIT. Tissue: Heart.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY256973 mRNA. Translation: AAP45003.1.
RefSeq	NP_858055.1.
UniGene	Bt.18851
3D structure databases
HSSP	HSSP built from PDB template 1H0K based on UniProtKB Q8WZM4.
SMR	Q7YS70. Positions 40-373.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000017253. Bos taurus. [Contig view]
GeneID	353301.
KEGG	bta:353301.
Phylogenomic databases
HOVERGEN	Q7YS70.
Family and domain databases
InterPro	IPR013154. AlcDHase_GroES-like. IPR002085. AlcDHase_SF_Zn. IPR013149. AlcDHase_Zn-bd. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MECR_BOVIN

Accession

Primary (citable) accession number: Q7YS70

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	October 1, 2003
Last modified:	November 25, 2008
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)