Reviewed, UniProtKB/Swiss-Prot Q7YS61 (TRDMT_BOVIN)

Names and origin

Protein names

Recommended name:
    tRNA (cytosine-5-)-methyltransferase
    EC=2.1.1.29
Alternative name(s):
    DNA (cytosine-5)-methyltransferase-like protein 2
      Short name=Dnmt2

Gene names

Name:	TRDMT1
Synonyms:	DNMT2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp) By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing 5-methylcytosine.
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q7YS61-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q7YS61-2)

The sequence of this isoform differs from the canonical sequence as follows:
     360-391: FPEMTTVKQRYRLLGNSLNVHVVAKLIKILCD → MWDTRGPQLSFINGLRT

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 391	391	tRNA (cytosine-5-)-methyltransferase		PRO_0000247560
Sites
	Active site	79	1	By similarity
Natural variations
	Alternative sequence	360 – 391	32	FPEMT…KILCD → MWDTRGPQLSFINGLRT in isoform 2.		VSP_020573

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: FDB6EE432C98265C	FASTA	391	44,570
	10 20 30 40 50 60 MEPLRALELY SGIGGMHQAL RESCIPAQVV AAVDVNTVAN EVYKYNFPHT QLLAKTIEGI 70 80 90 100 110 120 TLEEFDRLSF NMILMSPPCQ PFTRIGLQGD VTDPRTNSFL HILDILPRLQ KLPKYILLEN 130 140 150 160 170 180 VKGFEMSSTR DLLIQTIENC GFQYQEFLLS PTSLGIPNSR LRYFLIAKLQ PEPFPFQAPG 190 200 210 220 230 240 QVLMEFPKTE SEHPPKYAIN AEKKTEEKKT GPKICFDSST QCSGKEAILF KLETAGEIDR 250 260 270 280 290 300 KHQQDSDLSV RMLKDFLEDD IDKHSFFLPP KSLLRYALLL DIVKPTSRRS MCFTKGYGRY 310 320 330 340 350 360 IEGTGSVLQT TEDVQIENIY KSLTSLSQEE KIMRLSMLQL RFFTPKEIAN LLGFPPEFGF 370 380 390 PEMTTVKQRY RLLGNSLNVH VVAKLIKILC D
	Isoform 2. Checksum: D19F839DDFE14C53	FASTA	376	42,862
	10 20 30 40 50 60 MEPLRALELY SGIGGMHQAL RESCIPAQVV AAVDVNTVAN EVYKYNFPHT QLLAKTIEGI 70 80 90 100 110 120 TLEEFDRLSF NMILMSPPCQ PFTRIGLQGD VTDPRTNSFL HILDILPRLQ KLPKYILLEN 130 140 150 160 170 180 VKGFEMSSTR DLLIQTIENC GFQYQEFLLS PTSLGIPNSR LRYFLIAKLQ PEPFPFQAPG 190 200 210 220 230 240 QVLMEFPKTE SEHPPKYAIN AEKKTEEKKT GPKICFDSST QCSGKEAILF KLETAGEIDR 250 260 270 280 290 300 KHQQDSDLSV RMLKDFLEDD IDKHSFFLPP KSLLRYALLL DIVKPTSRRS MCFTKGYGRY 310 320 330 340 350 360 IEGTGSVLQT TEDVQIENIY KSLTSLSQEE KIMRLSMLQL RFFTPKEIAN LLGFPPEFGM 370 WDTRGPQLSF INGLRT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of DNA (cytosine 5) methyltransferase mRNA sequence and expression in bovine preimplantation embryos, fetal and adult tissues." Golding M.C., Westhusin M.E. Gene Expr. Patterns 3:551-558(2003) [PubMed: 12971987] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY244708 mRNA. Translation: AAP20550.1. BT021768 mRNA. Translation: AAX46615.1.
IPI	IPI00708300. IPI00717277.
RefSeq	NP_861528.1.
UniGene	Bt.22960
3D structure databases
SMR	Q7YS61. Positions 2-390.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7YS61.
Genome annotation databases
Ensembl	ENSBTAT00000027378; ENSBTAP00000027378; ENSBTAG00000020546; Bos taurus. [Genome view] ENSBTAT00000027381; ENSBTAP00000027381; ENSBTAG00000020546; Bos taurus. [Genome view]
GeneID	353353.
KEGG	bta:353353.
Organism-specific databases
CTD	353353.
Phylogenomic databases
eggNOG	maNOG12697.
HOVERGEN	Q7YS61.
InParanoid	Q7YS61.
PhylomeDB	Q7YS61.
Enzyme and pathway databases
BRENDA	2.1.1.29. 251.
Family and domain databases
InterPro	IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. [Graphical view]
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. False negative. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRDMT_BOVIN

Accession

Primary (citable) accession number: Q7YS61
Secondary accession number(s): Q58D29

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	October 1, 2003
Last modified:	February 9, 2010
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)