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Protein

tRNA (cytosine-5-)-methyltransferase

Gene

TRDMT1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp).By similarity

Catalytic activityi

S-adenosyl-L-methionine + cytosine(38) in tRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(38) in tRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei79 – 791PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. RNA binding Source: UniProtKB-KW
  3. tRNA methyltransferase activity Source: Ensembl

GO - Biological processi

  1. DNA methylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (cytosine-5-)-methyltransferase (EC:2.1.1.204)
Alternative name(s):
DNA (cytosine-5)-methyltransferase-like protein 2
Short name:
Dnmt2
Gene namesi
Name:TRDMT1
Synonyms:DNMT2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 13

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 391391tRNA (cytosine-5-)-methyltransferasePRO_0000247560Add
BLAST

Proteomic databases

PRIDEiQ7YS61.

Structurei

3D structure databases

ProteinModelPortaliQ7YS61.
SMRiQ7YS61. Positions 2-390.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 391388SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0270.
GeneTreeiENSGT00390000016416.
HOGENOMiHOG000265038.
HOVERGENiHBG051385.
InParanoidiQ7YS61.
KOiK15336.
OMAiQSFDINT.
OrthoDBiEOG7PZRZ4.
TreeFamiTF300024.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7YS61-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPLRALELY SGIGGMHQAL RESCIPAQVV AAVDVNTVAN EVYKYNFPHT
60 70 80 90 100
QLLAKTIEGI TLEEFDRLSF NMILMSPPCQ PFTRIGLQGD VTDPRTNSFL
110 120 130 140 150
HILDILPRLQ KLPKYILLEN VKGFEMSSTR DLLIQTIENC GFQYQEFLLS
160 170 180 190 200
PTSLGIPNSR LRYFLIAKLQ PEPFPFQAPG QVLMEFPKTE SEHPPKYAIN
210 220 230 240 250
AEKKTEEKKT GPKICFDSST QCSGKEAILF KLETAGEIDR KHQQDSDLSV
260 270 280 290 300
RMLKDFLEDD IDKHSFFLPP KSLLRYALLL DIVKPTSRRS MCFTKGYGRY
310 320 330 340 350
IEGTGSVLQT TEDVQIENIY KSLTSLSQEE KIMRLSMLQL RFFTPKEIAN
360 370 380 390
LLGFPPEFGF PEMTTVKQRY RLLGNSLNVH VVAKLIKILC D
Length:391
Mass (Da):44,570
Last modified:October 1, 2003 - v1
Checksum:iFDB6EE432C98265C
GO
Isoform 2 (identifier: Q7YS61-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     360-391: FPEMTTVKQRYRLLGNSLNVHVVAKLIKILCD → MWDTRGPQLSFINGLRT

Show »
Length:376
Mass (Da):42,862
Checksum:iD19F839DDFE14C53
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei360 – 39132FPEMT…KILCD → MWDTRGPQLSFINGLRT in isoform 2. 1 PublicationVSP_020573Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY244708 mRNA. Translation: AAP20550.1.
BT021768 mRNA. Translation: AAX46615.1.
RefSeqiNP_861528.1. NM_181812.2. [Q7YS61-1]
UniGeneiBt.22960.

Genome annotation databases

EnsembliENSBTAT00000027378; ENSBTAP00000027378; ENSBTAG00000020546. [Q7YS61-1]
GeneIDi353353.
KEGGibta:353353.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY244708 mRNA. Translation: AAP20550.1.
BT021768 mRNA. Translation: AAX46615.1.
RefSeqiNP_861528.1. NM_181812.2. [Q7YS61-1]
UniGeneiBt.22960.

3D structure databases

ProteinModelPortaliQ7YS61.
SMRiQ7YS61. Positions 2-390.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ7YS61.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000027378; ENSBTAP00000027378; ENSBTAG00000020546. [Q7YS61-1]
GeneIDi353353.
KEGGibta:353353.

Organism-specific databases

CTDi1787.

Phylogenomic databases

eggNOGiCOG0270.
GeneTreeiENSGT00390000016416.
HOGENOMiHOG000265038.
HOVERGENiHBG051385.
InParanoidiQ7YS61.
KOiK15336.
OMAiQSFDINT.
OrthoDBiEOG7PZRZ4.
TreeFamiTF300024.

Miscellaneous databases

NextBioi20812736.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of DNA (cytosine 5) methyltransferase mRNA sequence and expression in bovine preimplantation embryos, fetal and adult tissues."
    Golding M.C., Westhusin M.E.
    Gene Expr. Patterns 3:551-558(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).

Entry informationi

Entry nameiTRDMT_BOVIN
AccessioniPrimary (citable) accession number: Q7YS61
Secondary accession number(s): Q58D29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: October 1, 2003
Last modified: January 7, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.