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Protein

Glucose-6-phosphate 1-dehydrogenase

Gene

G6PD

Organism
Bos indicus (Zebu)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis (By similarity).By similarity

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

Pathwayi: pentose phosphate pathway

This protein is involved in step 1 of the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glucose-6-phosphate 1-dehydrogenase (G6PD)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
This subpathway is part of the pathway pentose phosphate pathway, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei147NADP 1By similarity1
Binding sitei171NADP 1; via carbonyl oxygenBy similarity1
Binding sitei171SubstrateBy similarity1
Binding sitei239SubstrateBy similarity1
Binding sitei258SubstrateBy similarity1
Binding sitei357NADP 2By similarity1
Binding sitei360SubstrateBy similarity1
Binding sitei365SubstrateBy similarity1
Binding sitei366NADP 2By similarity1
Binding sitei370NADP 2By similarity1
Binding sitei393NADP 2By similarity1
Binding sitei395SubstrateBy similarity1
Binding sitei487NADP 2By similarity1
Binding sitei503NADP 2By similarity1
Binding sitei509NADP 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi38 – 45NADP 1By similarity8
Nucleotide bindingi401 – 403NADP 2By similarity3
Nucleotide bindingi421 – 423NADP 2By similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00115; UER00408.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenase (EC:1.1.1.49)
Short name:
G6PD
Gene namesi
Name:G6PD
OrganismiBos indicus (Zebu)
Taxonomic identifieri9915 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000680802 – 515Glucose-6-phosphate 1-dehydrogenaseAdd BLAST514

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei8PhosphoserineBy similarity1
Modified residuei10PhosphothreonineBy similarity1
Modified residuei89N6-acetyllysineBy similarity1
Modified residuei171N6-acetyllysineBy similarity1
Modified residuei403N6-acetyllysineBy similarity1
Modified residuei432N6-acetyllysineBy similarity1
Modified residuei497N6-acetyllysineBy similarity1
Modified residuei503PhosphotyrosineBy similarity1

Post-translational modificationi

Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiQ7YS37.

Interactioni

Subunit structurei

Homotetramer; dimer of dimers. Interacts with SIRT2; the interaction is enhanced by H2O2 treatment (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ7YS37.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni201 – 205Substrate bindingBy similarity5

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG000856.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD. 1 hit.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7YS37-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEQVALSQT QTCGILWEEL CQSYSFHQSD THIFIIMGAS GDLAKRNIYP
60 70 80 90 100
TIWWLFQDGL LPKDTFIVGY TDSHFTVANI RKQSEPFFKS TPEEEPKLEE
110 120 130 140 150
FFAHSSYMAS QYDDVASYEH LNSGMNALHQ GPQANCLFYL ALLPSGYRTV
160 170 180 190 200
TKNICDTCTS QTGWNRIIVE KPFGRDLQSS NQLSNHIASL FHEDQIYRID
210 220 230 240 250
HYLGKEVVQN LMVLRFVNRI LGPIWNRDNI ACMSFTFKEP FGTEGRWSYL
260 270 280 290 300
SESGIIWEVM QNPLLQILCL VAMEKPISTN SDNIRDDKVR VLKCISKVQV
310 320 330 340 350
SNVVLSQYME NPTEEGEATR GYPEDPRVPH GSTTDTFAAA VLYVENERWD
360 370 380 390 400
GVPFILRCGK ALNERKAEVR LQFRDVAGDI FRQQCKRNEL VIRVQPNEAV
410 420 430 440 450
YTKMMTKKPG MFFNPEESEL DLTYGNRYKN VKFPDAYERL ILDVFCGSQM
460 470 480 490 500
HFVRSDELRE AWRIFTPLLH HIEREKARPI PYVYGSRGPV EADELMKRVG
510
FQYEGTYKWV NPHKL
Length:515
Mass (Da):59,584
Last modified:January 23, 2007 - v3
Checksum:i2397324759DD9972
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ566767 mRNA. Translation: CAD97761.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ566767 mRNA. Translation: CAD97761.1.

3D structure databases

ProteinModelPortaliQ7YS37.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ7YS37.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG000856.

Enzyme and pathway databases

UniPathwayiUPA00115; UER00408.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD. 1 hit.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG6PD_BOSIN
AccessioniPrimary (citable) accession number: Q7YS37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: January 23, 2007
Last modified: October 5, 2016
This is version 75 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Pro-263 is present instead of the conserved His which is expected to act as the active site proton acceptor.Curated

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.