Q7YRZ7 (GRAA_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Granzyme A
EC=3.4.21.78

Gene names

Name:

GZMA

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Lys or Arg. Cleaves after 'Lys-31' APEX1 and destroys its oxidative repair activity. Involved in apoptosis By similarity.
Catalytic activity	Hydrolysis of proteins, including fibronectin, type IV collagen and nucleolin. Preferential cleavage: -Arg-|-Xaa-, -Lys-|-Xaa- >> -Phe-|-Xaa- in small molecule substrates.
Subunit structure	Interacts with APEX1 By similarity. Homodimer; disulfide-linked.
Subcellular location	Secreted By similarity. Cytoplasmic granule By similarity. Note: Cytoplasmic granules of cytolytic T-lymphocytes By similarity.
Sequence similarities	Belongs to the peptidase S1 family. Granzyme subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Apoptosis Cytolysis
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW cytolysis Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of DNA binding Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of endodeoxyribonuclease activity Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of oxidoreductase activity Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of apoptotic process Inferred from sequence or structural similarity. Source: UniProtKB proteolysis involved in cellular protein catabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	By similarity
	Propeptide	27 – 28	2	Activation peptide By similarity		PRO_0000027397
	Chain	29 – 258	230	Granzyme A		PRO_0000027398
Regions
	Domain	29 – 255	227	Peptidase S1
Sites
	Active site	67	1	Charge relay system By similarity
	Active site	112	1	Charge relay system By similarity
	Active site	211	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	169	1	N-linked (GlcNAc...) Potential
	Disulfide bond	52 ↔ 68		By similarity
	Disulfide bond	146 ↔ 217		By similarity
	Disulfide bond	178 ↔ 196		By similarity
	Disulfide bond	207 ↔ 230		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7YRZ7 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 042707AD8C87624E	FASTA	258	28,070
	10 20 30 40 50 60 MNIPFPFSFP PAICLLLIPG VFPVSCEGII GGNEVAPHTR RYMALIKGLK LCAGALIKEN 70 80 90 100 110 120 WVLTAAHCDL KGNPQVILGA HSTSHKEKLD QVFSIKKAIP YPCFDPQTFE GDLQLLQLEG 130 140 150 160 170 180 KATMTKAVGI LQLPRTEDDV KPHTKCHVAG WGSTKKDACQ MSNALREANV TVIDRKICND 190 200 210 220 230 240 AQHYNFNPVI DLSMICAGGR KGEDDSCEGD SGSPLICDNV FRGVTSFGKC GNPQKPGIYI 250 LLTKKHLNWI KKTIAGAI

References

[1]	Jenne D.E. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ544059 mRNA. Translation: CAD66427.1.
IPI	IPI00691287.
RefSeq	NP_001001142.1. NM_001001142.1.
UniGene	Bt.29672.
3D structure databases
ProteinModelPortal	Q7YRZ7.
SMR	Q7YRZ7. Positions 29-258.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7YRZ7.
Protein family/group databases
MEROPS	S01.135.
Proteomic databases
PRIDE	Q7YRZ7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000040181; ENSBTAP00000039959; ENSBTAG00000027865.
GeneID	407178.
KEGG	bta:407178.
Organism-specific databases
CTD	3001.
Phylogenomic databases
eggNOG	maNOG14912.
GeneTree	ENSGT00580000081367.
HOVERGEN	HBG013304.
InParanoid	Q7YRZ7.
OMA	HTKCHVA.
OrthoDB	EOG4SXNDS.
PhylomeDB	Q7YRZ7.
Family and domain databases
InterPro	IPR009003. Pept_cys/ser_Trypsin-like. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
KO	K01352.
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GRAA_BOVIN

Accession

Primary (citable) accession number: Q7YRZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 23, 2004
Last sequence update:	October 1, 2003
Last modified:	November 16, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program