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Protein

Granzyme A

Gene

GZMA

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent cell death with morphological features of apoptosis when delivered into the target cell through the immunological synapse. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA (By similarity).By similarity

Catalytic activityi

Hydrolysis of proteins, including fibronectin, type IV collagen and nucleolin. Preferential cleavage: -Arg-|-Xaa-, -Lys-|-Xaa- >> -Phe-|-Xaa- in small molecule substrates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei67 – 671Charge relay systemBy similarity
Active sitei112 – 1121Charge relay systemBy similarity
Active sitei211 – 2111Charge relay systemBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Apoptosis, Cytolysis

Protein family/group databases

MEROPSiS01.135.

Names & Taxonomyi

Protein namesi
Recommended name:
Granzyme A (EC:3.4.21.78)
Gene namesi
Name:GZMA
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 20

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626By similarityAdd
BLAST
Propeptidei27 – 282Activation peptideBy similarityPRO_0000027397
Chaini29 – 258230Granzyme APRO_0000027398Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi52 ↔ 68PROSITE-ProRule annotation
Disulfide bondi146 ↔ 217PROSITE-ProRule annotation
Glycosylationi169 – 1691N-linked (GlcNAc...)Sequence analysis
Disulfide bondi178 ↔ 196PROSITE-ProRule annotation
Disulfide bondi207 ↔ 230PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ7YRZ7.
PRIDEiQ7YRZ7.

Interactioni

Subunit structurei

Interacts with APEX1 (By similarity). Homodimer; disulfide-linked.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000039959.

Structurei

3D structure databases

ProteinModelPortaliQ7YRZ7.
SMRiQ7YRZ7. Positions 29-258.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 255227Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118895.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ7YRZ7.
KOiK01352.
OMAiPQTFEGD.
OrthoDBiEOG7W9RVH.
TreeFamiTF333630.

Family and domain databases

InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7YRZ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIPFPFSFP PAICLLLIPG VFPVSCEGII GGNEVAPHTR RYMALIKGLK
60 70 80 90 100
LCAGALIKEN WVLTAAHCDL KGNPQVILGA HSTSHKEKLD QVFSIKKAIP
110 120 130 140 150
YPCFDPQTFE GDLQLLQLEG KATMTKAVGI LQLPRTEDDV KPHTKCHVAG
160 170 180 190 200
WGSTKKDACQ MSNALREANV TVIDRKICND AQHYNFNPVI DLSMICAGGR
210 220 230 240 250
KGEDDSCEGD SGSPLICDNV FRGVTSFGKC GNPQKPGIYI LLTKKHLNWI

KKTIAGAI
Length:258
Mass (Da):28,070
Last modified:October 1, 2003 - v1
Checksum:i042707AD8C87624E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ544059 mRNA. Translation: CAD66427.1.
RefSeqiNP_001001142.1. NM_001001142.1.
UniGeneiBt.29672.

Genome annotation databases

EnsembliENSBTAT00000040181; ENSBTAP00000039959; ENSBTAG00000027865.
GeneIDi407178.
KEGGibta:407178.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ544059 mRNA. Translation: CAD66427.1.
RefSeqiNP_001001142.1. NM_001001142.1.
UniGeneiBt.29672.

3D structure databases

ProteinModelPortaliQ7YRZ7.
SMRiQ7YRZ7. Positions 29-258.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000039959.

Protein family/group databases

MEROPSiS01.135.

Proteomic databases

PaxDbiQ7YRZ7.
PRIDEiQ7YRZ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000040181; ENSBTAP00000039959; ENSBTAG00000027865.
GeneIDi407178.
KEGGibta:407178.

Organism-specific databases

CTDi3001.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118895.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ7YRZ7.
KOiK01352.
OMAiPQTFEGD.
OrthoDBiEOG7W9RVH.
TreeFamiTF333630.

Family and domain databases

InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Jenne D.E.
    Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiGRAA_BOVIN
AccessioniPrimary (citable) accession number: Q7YRZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: October 1, 2003
Last modified: June 8, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.