Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Malate dehydrogenase, cytoplasmic

Gene

MDH1

Organism
Felis catus (Cat) (Felis silvestris catus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei92 – 921SubstrateBy similarity
Binding sitei98 – 981SubstrateBy similarity
Binding sitei105 – 1051NADBy similarity
Binding sitei112 – 1121NADBy similarity
Binding sitei131 – 1311SubstrateBy similarity
Binding sitei162 – 1621SubstrateBy similarity
Active sitei187 – 1871Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 177NADBy similarity
Nucleotide bindingi129 – 1313NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, cytoplasmic (EC:1.1.1.37)
Alternative name(s):
Cytosolic malate dehydrogenase
Gene namesi
Name:MDH1
OrganismiFelis catus (Cat) (Felis silvestris catus)
Taxonomic identifieri9685 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis
ProteomesiUP000011712 Componenti: Chromosome A3

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 334333Malate dehydrogenase, cytoplasmicPRO_0000226736Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei110 – 1101N6-succinyllysineBy similarity
Modified residuei118 – 1181N6-acetyllysineBy similarity
Modified residuei121 – 1211N6-acetyllysineBy similarity
Modified residuei214 – 2141N6-succinyllysineBy similarity
Modified residuei241 – 2411PhosphoserineBy similarity
Modified residuei298 – 2981N6-acetyllysine; alternateBy similarity
Modified residuei298 – 2981N6-succinyllysine; alternateBy similarity
Modified residuei318 – 3181N6-succinyllysineBy similarity
Modified residuei333 – 3331PhosphoserineBy similarity

Post-translational modificationi

ISGylated.By similarity
Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi9685.ENSFCAP00000015559.

Structurei

3D structure databases

ProteinModelPortaliQ7YRU4.
SMRiQ7YRU4. Positions 2-334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

Phylogenomic databases

eggNOGiCOG0039.
GeneTreeiENSGT00530000063410.
HOVERGENiHBG006340.
InParanoidiQ7YRU4.
KOiK00025.
OMAiFVTTVQQ.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7YRU4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL
60 70 80 90 100
DGVLMELQDC ALPLLKDVIA TDKEDVAFKD LDVAILVGSM PRRDGMERKD
110 120 130 140 150
LLKANVKIFK CQGAALEKYA KKSVKVIVVG NPANTNCLTA CKSAPSIPKE
160 170 180 190 200
NFSCLTRLDH NRAKAQIALK LGVTSDDVKN VIIWGNHSST QYPDVSHAKV
210 220 230 240 250
KLHGKEVGVY DALKDDSWLK GEFITTVQQR GAAVIKARKL SSAMSAAKAI
260 270 280 290 300
CDHVRDIWFG TPEGEFVSMG IISDGNPYGV PDDLLYSFPV TIKNKTWKVV
310 320 330
EGLTINDFSR EKMDLTAKEL AEEKETAFEF LSSA
Length:334
Mass (Da):36,423
Last modified:January 23, 2007 - v3
Checksum:i31FEB6E50FF5D946
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB113364 mRNA. Translation: BAC78621.1.
RefSeqiNP_001009329.1. NM_001009329.1.

Genome annotation databases

EnsembliENSFCAT00000027082; ENSFCAP00000017047; ENSFCAG00000024183.
GeneIDi493924.
KEGGifca:493924.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB113364 mRNA. Translation: BAC78621.1.
RefSeqiNP_001009329.1. NM_001009329.1.

3D structure databases

ProteinModelPortaliQ7YRU4.
SMRiQ7YRU4. Positions 2-334.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9685.ENSFCAP00000015559.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSFCAT00000027082; ENSFCAP00000017047; ENSFCAG00000024183.
GeneIDi493924.
KEGGifca:493924.

Organism-specific databases

CTDi4190.

Phylogenomic databases

eggNOGiCOG0039.
GeneTreeiENSGT00530000063410.
HOVERGENiHBG006340.
InParanoidiQ7YRU4.
KOiK00025.
OMAiFVTTVQQ.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of feline cytosolic malate dehydrogenase."
    Nakamura M., Sasaki N., Arai T.
    Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiMDHC_FELCA
AccessioniPrimary (citable) accession number: Q7YRU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: January 23, 2007
Last modified: May 27, 2015
This is version 74 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.