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Q7YRU4 (MDHC_FELCA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Malate dehydrogenase, cytoplasmic
EC=1.1.1.37
Alternative name(s):
Cytosolic malate dehydrogenase
Gene names
Name:MDH1
OrganismFelis catus (Cat) (Felis silvestris catus) [Complete proteome]
Taxonomic identifier9685 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Feliformia › Felidae › Felinae › Felis

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity.

Post-translational modification

ISGylated By similarity.

Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation By similarity.

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
   PTMAcetylation
Phosphoprotein
Ubl conjugation
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcentrosome

Inferred from electronic annotation. Source: Compara

mitochondrion

Inferred from electronic annotation. Source: Compara

   Molecular_functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 334333Malate dehydrogenase, cytoplasmic
PRO_0000226736

Regions

Nucleotide binding11 – 177NAD By similarity
Nucleotide binding129 – 1313NAD By similarity

Sites

Active site1871Proton acceptor By similarity
Binding site921Substrate By similarity
Binding site981Substrate By similarity
Binding site1051NAD By similarity
Binding site1121NAD By similarity
Binding site1311Substrate By similarity
Binding site1621Substrate By similarity

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue1181N6-acetyllysine By similarity
Modified residue1211N6-acetyllysine By similarity
Modified residue2101Phosphotyrosine By similarity
Modified residue2411Phosphoserine By similarity
Modified residue2981N6-acetyllysine By similarity
Modified residue3331Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7YRU4 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 31FEB6E50FF5D946

FASTA33436,423
        10         20         30         40         50         60 
MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL DGVLMELQDC 

        70         80         90        100        110        120 
ALPLLKDVIA TDKEDVAFKD LDVAILVGSM PRRDGMERKD LLKANVKIFK CQGAALEKYA 

       130        140        150        160        170        180 
KKSVKVIVVG NPANTNCLTA CKSAPSIPKE NFSCLTRLDH NRAKAQIALK LGVTSDDVKN 

       190        200        210        220        230        240 
VIIWGNHSST QYPDVSHAKV KLHGKEVGVY DALKDDSWLK GEFITTVQQR GAAVIKARKL 

       250        260        270        280        290        300 
SSAMSAAKAI CDHVRDIWFG TPEGEFVSMG IISDGNPYGV PDDLLYSFPV TIKNKTWKVV 

       310        320        330 
EGLTINDFSR EKMDLTAKEL AEEKETAFEF LSSA

« Hide

References

[1]"Cloning and characterization of feline cytosolic malate dehydrogenase."
Nakamura M., Sasaki N., Arai T.
Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB113364 mRNA. Translation: BAC78621.1.
RefSeqNP_001009329.1. NM_001009329.1.

3D structure databases

ProteinModelPortalQ7YRU4.
SMRQ7YRU4. Positions 2-334.
ModBaseSearch...

Protein-protein interaction databases

STRING9685.ENSFCAP00000015559.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID493924.
KEGGfca:493924.

Organism-specific databases

CTD4190.

Phylogenomic databases

eggNOGCOG0039.
GeneTreeENSGT00530000063410.
HOVERGENHBG006340.
KOK00025.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR23382. PTHR23382. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMSSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMsTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMDHC_FELCA
AccessionPrimary (citable) accession number: Q7YRU4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: January 23, 2007
Last modified: May 29, 2013
This is version 64 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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