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Q7YRU3

- PLCZ1_PIG

UniProt

Q7YRU3 - PLCZ1_PIG

Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1

Gene

PLCZ

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. Triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.By similarity2 PublicationsCurated

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.By similarity

    Cofactori

    Calcium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei170 – 1701By similarityPROSITE-ProRule annotation
    Active sitei215 – 2151By similarityPROSITE-ProRule annotation

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
    3. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. calcium ion transport Source: InterPro
    2. egg activation Source: InterPro
    3. intracellular signal transduction Source: InterPro
    4. lipid catabolic process Source: UniProtKB-KW
    5. multicellular organismal development Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein, Hydrolase, Transducer

    Keywords - Biological processi

    Fertilization, Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    Calcium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 (EC:3.1.4.11)
    Alternative name(s):
    Phosphoinositide phospholipase C-zeta-1
    Phospholipase C-zeta-1By similarity
    Short name:
    PLC-zeta-1By similarity
    Gene namesi
    Name:PLCZImported
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Unplaced

    Subcellular locationi

    Nucleus By similarity. Cytoplasmperinuclear region By similarity
    Note: Exhibits alternative cytoplasmic/nuclear localization during development. Translocates from the pronucleus into cytoplasm upon nuclear envelope breakdown for mitosis and localizes again to the pronucleus at interphase following meiosis and mitosis By similarity.By similarity

    GO - Cellular componenti

    1. cytosol Source: InterPro
    2. nucleus Source: UniProtKB-SubCell
    3. perinuclear region of cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 6366361-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1PRO_0000347247Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed specifically in testis.1 Publication

    Developmental stagei

    In testes, detected only when the elongated spermatids have differentiated from 96 days after birth.1 Publication

    Interactioni

    Subunit structurei

    Interacts via its C2 domain with PtdIns3P and, to a lesser extent, PtdIns5P in vitro.By similarity

    Protein-protein interaction databases

    STRINGi9823.ENSSSCP00000000621.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7YRU3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini35 – 7036EF-handSequence AnalysisAdd
    BLAST
    Domaini155 – 299145PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini375 – 491117PI-PLC Y-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini496 – 600105C2PROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili318 – 34528Sequence AnalysisAdd
    BLAST

    Domaini

    The EF-hand and C2 domains are essential for triggering Ca2+ oscillating activity and the regulation of PLCZ1 enzyme activity.By similarity
    The X-Y linker region between PI-PLC X-box and Y-box domains may be a target for proteolysis and may play an important regulatory role during fertilization.By similarity

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 EF-hand domain.Sequence Analysis
    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG149692.
    HOGENOMiHOG000006871.
    HOVERGENiHBG053610.
    KOiK05861.

    Family and domain databases

    Gene3Di1.10.238.10. 2 hits.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR001192. PI-PLC_fam.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR028395. PLC-zeta1.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view]
    PANTHERiPTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF29. PTHR10336:SF29. 1 hit.
    PfamiPF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view]
    PRINTSiPR00390. PHPHLIPASEC.
    SMARTiSM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7YRU3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MENKWFLSMV RDDFKGGKIN LEKAQKLLEK LDIQCNTIHV KCIFKDNDRL    50
    KQGRITIEEF RTIYRIIAHR EEIIEIFNAY PENRKILFER NLIDFLTQEQ 100
    YSLDINRSIV YEIIQKYEPI EEVKQAHQMS FEGFTRDMGS SECLLFNNEC 150
    GSVYQDMTHP LSDYFISSSH NTYLISDQIM GPSNLWGYVS ALVKGCRCLE 200
    IDCWDGSQNE PVVYHGYTLT SKLLFKTVIQ AIHKYAFITS DYPVVLSLEN 250
    HCSLSQQEVM ADNLQSVFGD ALLSDVLDDC PDRLPSPEAL KFKILVRNKK 300
    IGTLKETHER KGFDKHGQVQ ECEEEEEAEQ EEEENEVRDS EILDILQDDL 350
    EKEELKRGVG IKFFKKKKVK IATALSDLVI YTKVEKFRSF HYSRLYQQFN 400
    ETNSIGETQA RKLSKLRASE FILHTRKFIT RIYPKATRAD SSNFNPQEFW 450
    NIGCQMVALN FQTPGLPMDL QNGKFLENGN SGYILKPHFL RDGKSIFNPN 500
    KAPINSNPIT LTIRLISGIQ LPPSYHSSSN KADTLVIIEI FGVPNDQMKQ 550
    QTRVIKKNAF SPRWNETFTF IIQVPELALI RFVVENQGLI TGNEFLGQYT 600
    LPVLCMNKGY RRVPLFSKMG ESLEPASLFI YVWYIR 636
    Length:636
    Mass (Da):73,702
    Last modified:October 1, 2003 - v1
    Checksum:iA558E05243ECF479
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB113581 mRNA. Translation: BAC78817.1.
    RefSeqiNP_999515.1. NM_214350.1.
    UniGeneiSsc.17349.

    Genome annotation databases

    GeneIDi397632.
    KEGGissc:397632.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB113581 mRNA. Translation: BAC78817.1 .
    RefSeqi NP_999515.1. NM_214350.1.
    UniGenei Ssc.17349.

    3D structure databases

    ProteinModelPortali Q7YRU3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9823.ENSSSCP00000000621.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 397632.
    KEGGi ssc:397632.

    Organism-specific databases

    CTDi 397632.

    Phylogenomic databases

    eggNOGi NOG149692.
    HOGENOMi HOG000006871.
    HOVERGENi HBG053610.
    KOi K05861.

    Family and domain databases

    Gene3Di 1.10.238.10. 2 hits.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR001192. PI-PLC_fam.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR028395. PLC-zeta1.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view ]
    PANTHERi PTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF29. PTHR10336:SF29. 1 hit.
    Pfami PF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    [Graphical view ]
    PRINTSi PR00390. PHPHLIPASEC.
    SMARTi SM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning, testicular postnatal expression, and oocyte-activating potential of porcine phospholipase Czeta."
      Yoneda A., Kashima M., Yoshida S., Terada K., Nakagawa S., Sakamoto A., Hayakawa K., Suzuki K., Ueda J., Watanabe T.
      Reproduction 132:393-401(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: Landrace1 Publication.
      Tissue: Testis1 Publication.
    2. "Functional, biochemical, and chromatographic characterization of the complete [Ca2+]i oscillation-inducing activity of porcine sperm."
      Kurokawa M., Sato K., Wu H., He C., Malcuit C., Black S.J., Fukami K., Fissore R.A.
      Dev. Biol. 285:376-392(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiPLCZ1_PIG
    AccessioniPrimary (citable) accession number: Q7YRU3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 2, 2008
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3