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Q7YRU3

- PLCZ1_PIG

UniProt

Q7YRU3 - PLCZ1_PIG

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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1

Gene
PLCZ
Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. Triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function.By similarity2 Publications

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.By similarity

Cofactori

Calcium By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei170 – 1701 By similarityBy similarity
Active sitei215 – 2151 By similarityBy similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
  3. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. calcium ion transport Source: InterPro
  2. egg activation Source: InterPro
  3. intracellular signal transduction Source: InterPro
  4. lipid catabolic process Source: UniProtKB-KW
  5. multicellular organismal development Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Transducer

Keywords - Biological processi

Fertilization, Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C-zeta-1
Phospholipase C-zeta-1
Short name:
PLC-zeta-1
Gene namesi
Name:PLCZ
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Unplaced

Subcellular locationi

Nucleus By similarity. Cytoplasmperinuclear region By similarity
Note: Exhibits alternative cytoplasmic/nuclear localization during development. Translocates from the pronucleus into cytoplasm upon nuclear envelope breakdown for mitosis and localizes again to the pronucleus at interphase following meiosis and mitosis By similarity.By similarity

GO - Cellular componenti

  1. cytosol Source: InterPro
  2. nucleus Source: UniProtKB-SubCell
  3. perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6366361-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1PRO_0000347247Add
BLAST

Expressioni

Tissue specificityi

Expressed specifically in testis.1 Publication

Developmental stagei

In testes, detected only when the elongated spermatids have differentiated from 96 days after birth.1 Publication

Interactioni

Subunit structurei

Interacts via its C2 domain with PtdIns3P and, to a lesser extent, PtdIns5P in vitro By similarity.By similarity

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000000621.

Structurei

3D structure databases

ProteinModelPortaliQ7YRU3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 7036EF-handAdd
BLAST
Domaini155 – 299145PI-PLC X-boxAdd
BLAST
Domaini375 – 491117PI-PLC Y-boxAdd
BLAST
Domaini496 – 600105C2Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili318 – 34528 Reviewed predictionAdd
BLAST

Domaini

The EF-hand and C2 domains are essential for triggering Ca2+ oscillating activity and the regulation of PLCZ1 enzyme activity By similarity.By similarity
The X-Y linker region between PI-PLC X-box and Y-box domains may be a target for proteolysis and may play an important regulatory role during fertilization By similarity.By similarity

Sequence similaritiesi

Contains 1 C2 domain.
Contains 1 EF-hand domain.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG149692.
HOGENOMiHOG000006871.
HOVERGENiHBG053610.
KOiK05861.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR028395. PLC-zeta1.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF29. PTHR10336:SF29. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view]
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7YRU3-1 [UniParc]FASTAAdd to Basket

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MENKWFLSMV RDDFKGGKIN LEKAQKLLEK LDIQCNTIHV KCIFKDNDRL    50
KQGRITIEEF RTIYRIIAHR EEIIEIFNAY PENRKILFER NLIDFLTQEQ 100
YSLDINRSIV YEIIQKYEPI EEVKQAHQMS FEGFTRDMGS SECLLFNNEC 150
GSVYQDMTHP LSDYFISSSH NTYLISDQIM GPSNLWGYVS ALVKGCRCLE 200
IDCWDGSQNE PVVYHGYTLT SKLLFKTVIQ AIHKYAFITS DYPVVLSLEN 250
HCSLSQQEVM ADNLQSVFGD ALLSDVLDDC PDRLPSPEAL KFKILVRNKK 300
IGTLKETHER KGFDKHGQVQ ECEEEEEAEQ EEEENEVRDS EILDILQDDL 350
EKEELKRGVG IKFFKKKKVK IATALSDLVI YTKVEKFRSF HYSRLYQQFN 400
ETNSIGETQA RKLSKLRASE FILHTRKFIT RIYPKATRAD SSNFNPQEFW 450
NIGCQMVALN FQTPGLPMDL QNGKFLENGN SGYILKPHFL RDGKSIFNPN 500
KAPINSNPIT LTIRLISGIQ LPPSYHSSSN KADTLVIIEI FGVPNDQMKQ 550
QTRVIKKNAF SPRWNETFTF IIQVPELALI RFVVENQGLI TGNEFLGQYT 600
LPVLCMNKGY RRVPLFSKMG ESLEPASLFI YVWYIR 636
Length:636
Mass (Da):73,702
Last modified:October 1, 2003 - v1
Checksum:iA558E05243ECF479
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB113581 mRNA. Translation: BAC78817.1.
RefSeqiNP_999515.1. NM_214350.1.
UniGeneiSsc.17349.

Genome annotation databases

GeneIDi397632.
KEGGissc:397632.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB113581 mRNA. Translation: BAC78817.1 .
RefSeqi NP_999515.1. NM_214350.1.
UniGenei Ssc.17349.

3D structure databases

ProteinModelPortali Q7YRU3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9823.ENSSSCP00000000621.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 397632.
KEGGi ssc:397632.

Organism-specific databases

CTDi 397632.

Phylogenomic databases

eggNOGi NOG149692.
HOGENOMi HOG000006871.
HOVERGENi HBG053610.
KOi K05861.

Family and domain databases

Gene3Di 1.10.238.10. 2 hits.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProi IPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR001192. PI-PLC_fam.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR028395. PLC-zeta1.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view ]
PANTHERi PTHR10336. PTHR10336. 1 hit.
PTHR10336:SF29. PTHR10336:SF29. 1 hit.
Pfami PF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
[Graphical view ]
PRINTSi PR00390. PHPHLIPASEC.
SMARTi SM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF51695. SSF51695. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning, testicular postnatal expression, and oocyte-activating potential of porcine phospholipase Czeta."
    Yoneda A., Kashima M., Yoshida S., Terada K., Nakagawa S., Sakamoto A., Hayakawa K., Suzuki K., Ueda J., Watanabe T.
    Reproduction 132:393-401(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: Landrace.
    Tissue: Testis.
  2. "Functional, biochemical, and chromatographic characterization of the complete [Ca2+]i oscillation-inducing activity of porcine sperm."
    Kurokawa M., Sato K., Wu H., He C., Malcuit C., Black S.J., Fukami K., Fissore R.A.
    Dev. Biol. 285:376-392(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPLCZ1_PIG
AccessioniPrimary (citable) accession number: Q7YRU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: October 1, 2003
Last modified: April 16, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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