Reviewed, UniProtKB/Swiss-Prot Q7YRH6 (PEMT_BOVIN)

Names and origin

Protein names

Recommended name:
    Phosphatidylethanolamine N-methyltransferase
      Short name=PEAMT
      Short name=PEMT
    EC=2.1.1.17
Alternative name(s):
    PEMT2

Gene names

Name:	PEMT
Synonyms:	PEMT2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	199 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes three sequential methylation of phosphatidylethanolamine (PE) by AdoMet, thus producing phosphatidylcholine (PC) By similarity.
Catalytic activity	S-adenosyl-L-methionine + phosphatidylethanolamine = S-adenosyl-L-homocysteine + phosphatidyl-N-methylethanolamine.
Enzyme regulation	The first methylation is rate-limiting By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Potential. Mitochondrion membrane; Multi-pass membrane protein Potential. Note: Found in endoplasmic reticulum where most PEMT activity is generated and in mitochondria By similarity.
Sequence similarities	Belongs to the PEMT/PEM2 methyltransferase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Endoplasmic reticulum Membrane Mitochondrion
Domain	Transmembrane
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphatidylethanolamine N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 199	198	Phosphatidylethanolamine N-methyltransferase		PRO_0000281924
Regions
	Transmembrane	13 – 33	21	Potential
	Transmembrane	46 – 66	21	Potential
	Transmembrane	94 – 114	21	Potential
	Transmembrane	158 – 178	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q7YRH6-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: E6F91D9AF795B2CC	FASTA	199	22,018
	10 20 30 40 50 60 MTRLLGYVDL SEPHFVAAVL AIVFNPLFWN VVARWEHKTR KLSKAFGSPR LACYTLGGAI 70 80 90 100 110 120 LLLNVLRSHC FTQAMLSQPR MQSLDNPAVY HVGLALLGVG GVFVLSSFLA LGFTGTFLGD 130 140 150 160 170 180 YFGILKEARV TMFPFSVLDN PMYWGSTAIY LGWAIVHASP TGLLLTALVA LIYMVAIVYE 190 EPFTAEIYQQ KASQAYKRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of Bos taurus pemt2." Zhou G., Yu L., Zhao S. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY334572 mRNA. Translation: AAQ01191.1. BC105466 mRNA. Translation: AAI05467.1.
IPI	IPI00701113.
RefSeq	NP_892034.1.
UniGene	Bt.20969
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000016432. Bos taurus. [Contig view]
GeneID	360197.
KEGG	bta:360197.
Phylogenomic databases
HOVERGEN	Q7YRH6.
Enzyme and pathway databases
BRENDA	2.1.1.17. 251.
Family and domain databases
InterPro	IPR007318. PEMT. [Graphical view]
PANTHER	PTHR15458. PEMT. 1 hit.
Pfam	PF04191. PEMT. 1 hit. [Graphical view]
PIRSF	PIRSF005444. PEMT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PEMT_BOVIN

Accession

Primary (citable) accession number: Q7YRH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	October 1, 2003
Last modified:	February 10, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)