Reviewed, UniProtKB/Swiss-Prot Q7YRB7 (AOFB_CANFA)

Names and origin

Protein names

Recommended name:
    Amine oxidase [flavin-containing] B
    EC=1.4.3.4
Alternative name(s):
    Monoamine oxidase type B
      Short name=MAO-B

Gene names

Name:

MAOB

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine By similarity.
Catalytic activity	RCH2NHR' + H2O + O2 = RCHO + R'NH2 + H2O2.
Cofactor	FAD By similarity.
Subunit structure	Monomer, homo- or heterodimer (containing two subunits of similar size). Each subunit contains a covalently bound flavin. Enzymatically active as monomer By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the flavin monoamine oxidase family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Acetylation
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amine oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 520	519	Amine oxidase [flavin-containing] B		PRO_0000099857
Regions
	Topological domain	2 – 489	488	Cytoplasmic By similarity
	Transmembrane	490 – 516	27	Anchor for type IV membrane protein By similarity
	Topological domain	517 – 520	4	Mitochondrial intermembrane By similarity
	Compositional bias	36 – 52	17	Arg/Lys-rich (basic)
Sites
	Site	156	1	Important for catalytic activity By similarity
	Site	365	1	Important for catalytic activity By similarity
	Site	382	1	Important for catalytic activity By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	52	1	N6-acetyllysine By similarity
	Modified residue	397	1	S-8alpha-FAD cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7YRB7-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: D5C6295875D7D8BE	FASTA	520	58,384
	10 20 30 40 50 60 MSGKCDVVVV GGGISGMAAA KLLHDFGLNV VVLEARDRVG GRTYTIRNQK VKYLDLGGSY 70 80 90 100 110 120 VGPTQNCILR LAKELGLETY KVNEVERLIH HVKGKSYPFR GPFPPVWNPI AYLDHNNLWR 130 140 150 160 170 180 TMDDMGREIP SDAPWKAPLA EEWDHMTMKE LLDKICWTES AKQLATLFVN LCVTAETHEV 190 200 210 220 230 240 SALWFLWYVK QCGGTTRIIS TTNGGQERKF VGGSGQVSER IMDLLGDQVK LERPVTHIDQ 250 260 270 280 290 300 TGENVLVETL NHEVYEAKYV ISAIPPTLGM KIHFNPPLPM MRNQLITRVP LGSVIKCIVY 310 320 330 340 350 360 YKEPFWRKKD YCGTMIIEGE EAPIAYTLDD TKPDGNYAAI MGFILAHKAR KLARLTKDER 370 380 390 400 410 420 MKKLCELYAK VLGSQEALQP VHYEEKNWCE EQYSGGCYTT YFPPGIMTQY GRVLRQPVGR 430 440 450 460 470 480 IYFAGTETAT HWSGYMEGAV EAGERAAREI LHAMGKIPED EIWQSEPESV DVPAQPITTT 490 500 510 520 FLERHLPSVP GLLRLIGLTA IFSATALGVL AHKRGLLVRV

References

[1]	Hashizume C., Mori Y. Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Beagle. Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB070958 mRNA. Translation: BAB86936.1.
RefSeq	NP_001002970.1.
UniGene	Cfa.152
3D structure databases
SMR	Q7YRB7. Positions 4-499.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7YRB7.
Genome annotation databases
Ensembl	ENSCAFT00000022963; ENSCAFP00000021330; ENSCAFG00000014451; Canis familiaris. [Genome view]
GeneID	403451.
KEGG	cfa:403451.
Organism-specific databases
CTD	403451.
Phylogenomic databases
eggNOG	maNOG07443.
HOVERGEN	Q7YRB7.
Enzyme and pathway databases
BRENDA	1.4.3.4. 463.
Family and domain databases
InterPro	IPR001613. Amineoxid_fl. IPR002937. Amino_oxidase. [Graphical view]
Pfam	PF01593. Amino_oxidase. 1 hit. [Graphical view]
PRINTS	PR00757. AMINEOXDASEF.
ProtoNet	Search...

Entry information

Entry name

AOFB_CANFA

Accession

Primary (citable) accession number: Q7YRB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 49 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)