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Q7YR49

- PO5F1_PANTR

UniProt

Q7YR49 - PO5F1_PANTR

Protein

POU domain, class 5, transcription factor 1

Gene

POU5F1

Organism
Pan troglodytes (Chimpanzee)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi230 – 28960HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: Ensembl
    2. miRNA binding Source: Ensembl
    3. sequence-specific DNA binding Source: Ensembl
    4. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: Ensembl
    5. transcription corepressor activity Source: Ensembl
    6. transcription regulatory region DNA binding Source: Ensembl

    GO - Biological processi

    1. BMP signaling pathway involved in heart induction Source: Ensembl
    2. cardiac cell fate determination Source: Ensembl
    3. ectodermal cell fate commitment Source: Ensembl
    4. endodermal cell fate specification Source: Ensembl
    5. germ-line stem cell maintenance Source: Ensembl
    6. mesodermal cell fate commitment Source: Ensembl
    7. mRNA transcription from RNA polymerase II promoter Source: Ensembl
    8. negative regulation of calcium ion-dependent exocytosis Source: Ensembl
    9. negative regulation of gene silencing by miRNA Source: Ensembl
    10. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
    11. positive regulation of catenin import into nucleus Source: Ensembl
    12. positive regulation of SMAD protein import into nucleus Source: Ensembl
    13. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
    14. regulation of asymmetric cell division Source: Ensembl
    15. regulation of heart induction by regulation of canonical Wnt signaling pathway Source: Ensembl
    16. regulation of methylation-dependent chromatin silencing Source: Ensembl
    17. response to retinoic acid Source: Ensembl
    18. response to wounding Source: Ensembl
    19. somatic stem cell maintenance Source: Ensembl
    20. trophectodermal cell differentiation Source: Ensembl

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    POU domain, class 5, transcription factor 1
    Alternative name(s):
    Octamer-binding protein 3
    Short name:
    Oct-3
    Octamer-binding transcription factor 3
    Short name:
    OTF-3
    Gene namesi
    Name:POU5F1
    Synonyms:OCT3, OTF3
    OrganismiPan troglodytes (Chimpanzee)
    Taxonomic identifieri9598 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePan
    ProteomesiUP000002277: Chromosome 6

    Subcellular locationi

    Cytoplasm. Nucleus
    Note: Expressed in a diffuse and slightly punctuate pattern.By similarity

    GO - Cellular componenti

    1. cytosol Source: Ensembl
    2. nucleoplasm Source: Ensembl
    3. transcriptional repressor complex Source: Ensembl
    4. transcription factor complex Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 360360POU domain, class 5, transcription factor 1PRO_0000100750Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei111 – 1111Phosphoserine; by MAPKBy similarity
    Cross-linki123 – 123Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
    Modified residuei235 – 2351PhosphothreonineBy similarity
    Modified residuei236 – 2361PhosphoserineBy similarity
    Modified residuei289 – 2891PhosphoserineBy similarity
    Modified residuei290 – 2901PhosphoserineBy similarity
    Modified residuei355 – 3551PhosphoserineBy similarity

    Post-translational modificationi

    Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression By similarity.By similarity
    Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.By similarity
    ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription By similarity.By similarity

    Keywords - PTMi

    Isopeptide bond, Phosphoprotein, Ubl conjugation

    Interactioni

    Subunit structurei

    Interacts with ZSCAN10, UBE2I and PKM. Interacts with WWP2 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ7YR49.
    SMRiQ7YR49. Positions 144-289.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini138 – 21275POU-specificPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The POU-specific domain mediates interaction with PKM.By similarity

    Sequence similaritiesi

    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 POU-specific domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    eggNOGiNOG329627.
    GeneTreeiENSGT00750000117482.
    HOGENOMiHOG000089941.
    HOVERGENiHBG057998.
    InParanoidiQ7YR49.
    KOiK09367.
    OrthoDBiEOG7DJSMG.
    TreeFamiTF316413.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR015585. POU_dom_5.
    IPR000327. POU_specific.
    [Graphical view]
    PANTHERiPTHR11636:SF15. PTHR11636:SF15. 1 hit.
    PfamiPF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view]
    PRINTSiPR00028. POUDOMAIN.
    SMARTiSM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00035. POU_1. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7YR49-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGHLTSDFA FSPPPGGGGD GPGGPEPGWV DPRTWLSFQG PPGGPGIGPG    50
    VGPGSEVWGI PPCPPPYEFC GGMAYCGPQV GVGLVPQGGL ETSQPEGEAG 100
    VGVESNSDGA SPEPCTVTPG AVKLEKEKLE QNPEESQDIK ALQKELEQFA 150
    KLLKQKRITL GYTQADVGLT LGVLFGKVFS QTTICRFEAL QLSFKNMCKL 200
    RPLLQKWVEE ADNNENLQEI CKAETLVQAR KRKRTSIENR VRGNLENLFL 250
    QCPKPTLQQI SHIAQQLGLE KDVVRVWFCN RRQKGKRSSS DYAQREDFEA 300
    AGSPFSGGPV SFPLAPGPHF GTPGYGSPHF TALYSSVPFP EGEAFPPVSV 350
    TTLGSPMHSN 360
    Length:360
    Mass (Da):38,601
    Last modified:October 1, 2003 - v1
    Checksum:i375D4ADB3C01348C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti6 – 61T → A in BAE92816. (PubMed:16702430)Curated
    Sequence conflicti6 – 61T → A in BAE92818. (PubMed:16702430)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000041 Genomic DNA. Translation: BAC78165.1.
    AB210197 Genomic DNA. Translation: BAE92816.1.
    AB210198 Genomic DNA. Translation: BAE92818.1.
    RefSeqiNP_001238970.1. NM_001252041.1.

    Genome annotation databases

    EnsembliENSPTRT00000033151; ENSPTRP00000030631; ENSPTRG00000017953.
    GeneIDi744263.
    KEGGiptr:744263.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000041 Genomic DNA. Translation: BAC78165.1 .
    AB210197 Genomic DNA. Translation: BAE92816.1 .
    AB210198 Genomic DNA. Translation: BAE92818.1 .
    RefSeqi NP_001238970.1. NM_001252041.1.

    3D structure databases

    ProteinModelPortali Q7YR49.
    SMRi Q7YR49. Positions 144-289.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSPTRT00000033151 ; ENSPTRP00000030631 ; ENSPTRG00000017953 .
    GeneIDi 744263.
    KEGGi ptr:744263.

    Organism-specific databases

    CTDi 5460.

    Phylogenomic databases

    eggNOGi NOG329627.
    GeneTreei ENSGT00750000117482.
    HOGENOMi HOG000089941.
    HOVERGENi HBG057998.
    InParanoidi Q7YR49.
    KOi K09367.
    OrthoDBi EOG7DJSMG.
    TreeFami TF316413.

    Miscellaneous databases

    NextBioi 20911216.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR015585. POU_dom_5.
    IPR000327. POU_specific.
    [Graphical view ]
    PANTHERi PTHR11636:SF15. PTHR11636:SF15. 1 hit.
    Pfami PF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view ]
    PRINTSi PR00028. POUDOMAIN.
    SMARTi SM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00035. POU_1. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparative sequencing of human and chimpanzee MHC class I regions unveils insertions/deletions as the major path to genomic divergence."
      Anzai T., Shiina T., Kimura N., Yanagiya K., Kohara S., Shigenari A., Yamagata T., Kulski J.K., Naruse T.K., Fujimori Y., Fukuzumi Y., Yamazaki M., Tashiro H., Iwamoto C., Umehara Y., Imanishi T., Meyer A., Ikeo K.
      , Gojobori T., Bahram S., Inoko H.
      Proc. Natl. Acad. Sci. U.S.A. 100:7708-7713(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "Rapid evolution of major histocompatibility complex class I genes in primates generates new disease alleles in humans via hitchhiking diversity."
      Shiina T., Ota M., Shimizu S., Katsuyama Y., Hashimoto N., Takasu M., Anzai T., Kulski J.K., Kikkawa E., Naruse T., Kimura N., Yanagiya K., Watanabe A., Hosomichi K., Kohara S., Iwamoto C., Umehara Y., Meyer A.
      , Wanner V., Sano K., Macquin C., Ikeo K., Tokunaga K., Gojobori T., Inoko H., Bahram S.
      Genetics 173:1555-1570(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiPO5F1_PANTR
    AccessioniPrimary (citable) accession number: Q7YR49
    Secondary accession number(s): Q1XHV2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 21, 2003
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3