Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mediator of RNA polymerase II transcription subunit 12

Gene

MED12

Organism
Pan troglodytes (Chimpanzee)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway (By similarity).By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 12
Alternative name(s):
Mediator complex subunit 12
Trinucleotide repeat-containing gene 11 protein
Gene namesi
Name:MED12
Synonyms:TNRC11
OrganismiPan troglodytes (Chimpanzee)
Taxonomic identifieri9598 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePan
Proteomesi
  • UP000002277 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 20272027Mediator of RNA polymerase II transcription subunit 12PRO_0000312958Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei13 – 131PhosphotyrosineBy similarity
Modified residuei482 – 4821PhosphoserineBy similarity
Modified residuei512 – 5121PhosphoserineBy similarity
Modified residuei545 – 5451PhosphoserineBy similarity
Modified residuei547 – 5471PhosphoserineBy similarity
Modified residuei1105 – 11051PhosphoserineBy similarity
Modified residuei1116 – 11161PhosphoserineBy similarity
Modified residuei1645 – 16451N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ7YQK8.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Also interacts with CTNNB1 and GLI3 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9598.ENSPTRP00000047374.

Structurei

3D structure databases

ProteinModelPortaliQ7YQK8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1463 – 1901439Interaction with CTNNB1 and GLI3By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1579 – 162446Pro-richAdd
BLAST
Compositional biasi1747 – 2018272Gln-richAdd
BLAST

Sequence similaritiesi

Belongs to the Mediator complex subunit 12 family.Curated

Phylogenomic databases

eggNOGiKOG3598. Eukaryota.
ENOG410XP2V. LUCA.
HOVERGENiHBG052447.
InParanoidiQ7YQK8.
KOiK15162.

Family and domain databases

InterProiIPR021989. Mediator_Med12_catenin-bd.
IPR021990. Mediator_Med12_LCEWAV.
[Graphical view]
PfamiPF12145. Med12-LCEWAV. 1 hit.
PF12144. Med12-PQL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7YQK8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAAWLIKMT CAYYAAISET KVKKRHVDPF MEWTQIITKY LWEQLQKMAE
60 70 80 90 100
YYRPGPAGSG GCGSTIGPLP HDVEVAIRQW DYTEKLAMFM FQDGMLDRHE
110 120 130 140 150
FLTWVLECFE KIRPGEDELL KLLLPLLLRY SGEFVQSAYL SRRLAYFCTR
160 170 180 190 200
RLALQLDGVS SHSSHVISAQ STSTLPTTPA PQPPTSSTPS TPFSDLLMCP
210 220 230 240 250
QHRPLVFGLS CILQTILLCC PSALVWHYSL TDSRIKTGSP LDHLPIAPSN
260 270 280 290 300
LPMPEGNSAF TQQVRAKLRE IEQQIKERGQ AVEVRWSFDK CQEATAGFTI
310 320 330 340 350
GRVLHTLEVL DSHSFERSDF SNSLDSLCNR IFGLGPSKDG HEISSDDDAV
360 370 380 390 400
VSLLCEWAVS CKRSGRHRAM VVAKLLEKRQ AEIEAERCGE SEAADEKGSI
410 420 430 440 450
ASGSLSAPSA PIFQDVLLQF LDTQAPMLTD PRSENERVEF FNLVLLFCEL
460 470 480 490 500
IRHDVFSHNM YTCTLISRGD LAFGAPGPRP PSPFDDPADD AEHKEAEGSS
510 520 530 540 550
SSKLEDPGLS ESMDIDPSSS VLFEDMEKPD FSLFSPTMPC EGKGSPSPEK
560 570 580 590 600
PDVEKEVKPP PKEKIEGTLG ILYDQPRHVQ YATHFPIPQE ESCSHECNQR
610 620 630 640 650
LVVLFGVGKQ RDDARHAIKK ITKDILKVLN RKGTAETDQL APIVPLNPGD
660 670 680 690 700
LTFLGGEDGQ KRRRNRPEAF PTAEDIFAKF QHLSHYDQHQ VTAQVSRNVL
710 720 730 740 750
EQITSFALGM SYHLPLVQHV QFIFDLMEYS LSISGLIDFA IQLLNELSVV
760 770 780 790 800
EAELLLKSSD LVGSYTTSLC LCIVAVLRHY HACLILNQDQ MAQVFEGLCG
810 820 830 840 850
VVKHGMNRSD GSSAERCILA YLYDLYTSCS HLKNKFGELF SDFCSKVKNT
860 870 880 890 900
IYCNVEPSES NMRWAPEFMI DTLENPAAHT FTYTGLGKSL SENPANRYSF
910 920 930 940 950
VCNALMHVCV GHHDPDRVND IAILCAELTG YCKSLSAEWL GVLKALCCSS
960 970 980 990 1000
NNGTCGFNDL LCNVDVSDLS FHDSLATFVA ILIARQCLLL EDLIRCAAIP
1010 1020 1030 1040 1050
SLLNAACSEQ DSEPGARLTC RILLHLFKTP QLNPCQSDGN KPTVGIRSSC
1060 1070 1080 1090 1100
DRHLLAASQN RIVDGAVFAV LKAVFVLGDA ELKGSGFTVT GGTEELPEEE
1110 1120 1130 1140 1150
GGGGSGGRRQ GGRNISVETA SLDVYAKYVL RSICQQEWVG ERCLKSLCED
1160 1170 1180 1190 1200
SNDLQDPVLS SAQAQRLMQL ICYPHRLLDN EDGENPQRQR IKRILQNLDQ
1210 1220 1230 1240 1250
WTMRQSSLEL QLMIKQTPNN EMNSLLENIA KATIEVFQQS AETGSSSGST
1260 1270 1280 1290 1300
ASNMPSSSKT KPVLSSLERS GVWLVAPLIA KLPTSVQGHV LKAAGEELEK
1310 1320 1330 1340 1350
GQHLGSSSRK ERDRQKQKSM SLLSQQPFLS LVLTCLKGQD EQREGLLTSL
1360 1370 1380 1390 1400
YSQVHQIVNN WRDDQYLDDC KPKQLMHEAL KLRLNLVGGM FDTVQRSTQQ
1410 1420 1430 1440 1450
TTEWAMLLLE IIISGTVDMQ SNNELFTTVL DMLSVLINGT LAADMSSISQ
1460 1470 1480 1490 1500
GSMEENKRAY MNLAKKLQKE LGERQSDSLE KVRQLLPLPK QTRDVITCEP
1510 1520 1530 1540 1550
QGSLIDTKGN KIAGFDSIFK KEGLQVSTKQ KISPWDLFEG LKPSAPLSWG
1560 1570 1580 1590 1600
WFGTVRVDRR VARGEEQQRL LLYHTHLRPR PRAYYLEPLP LPPEDEEPPA
1610 1620 1630 1640 1650
PTLLEPEKKA PEPPKTDKPG AAPPSTEERK KKSTKGKKRS QPATKTEDYG
1660 1670 1680 1690 1700
MGPGRSGPYG VTVPPDLLHH PNPGSITHLN YRQGSIGLYT QNQPLPAGGP
1710 1720 1730 1740 1750
RVDPYRPVRL PMQKLPTRPT YPGVLPTTMT GVMGLEPSSY KTSVYRQQQP
1760 1770 1780 1790 1800
AVPQGQRLRQ QLQAKIQSQG MLGQSSVHQM TPSSSYGLQT SQGYTPYVSH
1810 1820 1830 1840 1850
VGLQQHTGPA GTMVPPSYSS QPYQSTHPST NPTLVDPTRH LQQRPSGYVH
1860 1870 1880 1890 1900
QQAPTYGHGL TSTQRFSHQT LQQTPMISTM TPMSAQGVQA GVRSTAILPE
1910 1920 1930 1940 1950
QQQQQQQQQQ QQQQQQQQQQ QQQQQQYHIR QQQQQQILRQ QQQQQQQQQQ
1960 1970 1980 1990 2000
QQQQQQQQQQ QQQQQHQQQQ QQQAAPPQPQ PQSQPQFQRQ GLQQTQQQQQ
2010 2020
TAALVRQLQQ QLSNTQPQPS TNIFGRY
Length:2,027
Mass (Da):226,338
Last modified:October 1, 2003 - v1
Checksum:iD598B3534ED56057
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB102669 mRNA. Translation: BAC81138.1.
RefSeqiNP_001009019.1. NM_001009019.1.
UniGeneiPtr.6417.

Genome annotation databases

GeneIDi449626.
KEGGiptr:449626.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB102669 mRNA. Translation: BAC81138.1.
RefSeqiNP_001009019.1. NM_001009019.1.
UniGeneiPtr.6417.

3D structure databases

ProteinModelPortaliQ7YQK8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9598.ENSPTRP00000047374.

Proteomic databases

PaxDbiQ7YQK8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi449626.
KEGGiptr:449626.

Organism-specific databases

CTDi9968.

Phylogenomic databases

eggNOGiKOG3598. Eukaryota.
ENOG410XP2V. LUCA.
HOVERGENiHBG052447.
InParanoidiQ7YQK8.
KOiK15162.

Family and domain databases

InterProiIPR021989. Mediator_Med12_catenin-bd.
IPR021990. Mediator_Med12_LCEWAV.
[Graphical view]
PfamiPF12145. Med12-LCEWAV. 1 hit.
PF12144. Med12-PQL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMED12_PANTR
AccessioniPrimary (citable) accession number: Q7YQK8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 1, 2003
Last modified: July 6, 2016
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.