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Protein

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3

Gene

GCNT3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development.By similarity

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + O3-(beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl)-L-seryl/threonyl-[protein] = UDP + O3-(beta-D-galactosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->6))-N-acetyl-alpha-D-galactosaminyl)-L-seryl/threonyl-[protein].By similarity
UDP-N-acetyl-alpha-D-glucosamine + beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc-R = UDP + beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->3)-(beta-D-GlcNAc-(1->6))-beta-D-Gal-(1->4)-beta-D-GlcNAc-R.By similarity
UDP-N-acetyl-alpha-D-glucosamine + N-acetyl-beta-D-glucosaminyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->6)-(N-acetyl-beta-D-glucosaminyl-(1->3))-N-acetyl-alpha-D-galactosaminyl-R.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-BTA-913709 O-linked glycosylation of mucins
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT14 Glycosyltransferase Family 14

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 (EC:2.4.1.102By similarity, EC:2.4.1.148By similarity, EC:2.4.1.150By similarity)
Alternative name(s):
C2GnT-mucin type
Mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase
bC2GnT-M
Short name:
C2GnT-M
Gene namesi
Name:GCNT3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 10

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Transmembranei13 – 30Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST18
Topological domaini31 – 440LumenalSequence analysisAdd BLAST410

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002885431 – 440Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3Add BLAST440

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi72N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi73 ↔ 2301 Publication
Glycosylationi108N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi164 ↔ 3841 Publication
Disulfide bondi185 ↔ 2121 Publication
Disulfide bondi393 ↔ 4251 Publication

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ7YQE1
PRIDEiQ7YQE1

Expressioni

Tissue specificityi

Primarily expressed in mucus-secreting tissues.

Gene expression databases

BgeeiENSBTAG00000009443

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000012427

Structurei

3D structure databases

ProteinModelPortaliQ7YQE1
SMRiQ7YQE1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 14 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0799 Eukaryota
ENOG410XQ7M LUCA
GeneTreeiENSGT00760000119183
HOGENOMiHOG000293251
HOVERGENiHBG051711
InParanoidiQ7YQE1
KOiK09662
OMAiHLLANKF
OrthoDBiEOG091G0HLZ
TreeFamiTF315534

Family and domain databases

InterProiView protein in InterPro
IPR003406 Glyco_trans_14
PfamiView protein in Pfam
PF02485 Branch, 1 hit

Sequencei

Sequence statusi: Complete.

Q7YQE1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMTGWKKKL CRGHHLWALG CYMLLAVVAL RLSLRLKCDV DSLDLESRDF
60 70 80 90 100
QSQRCRDVLY KNLKLPAKRS INCSGITRGD QEAVVQALLD NLEVKKKRLP
110 120 130 140 150
FTDTYYLNIT RDCEQFKAQR KFIQFPLSKE ELDFPIAYSM VVHEKIENFE
160 170 180 190 200
RLLRAVYAPQ NIYCVHVDVK SPETFKEAVK AIISCFPNVF MASKLVPVVY
210 220 230 240 250
ASWSRVQADL NCMEDLLQSS VPWKYLLNTC GTDFPIKTNA EMVLALKMLN
260 270 280 290 300
GKNSMESEIP SEYKKNRWKY RYEVTDRLYL TSKMKDPPPD NLPMFTGNAY
310 320 330 340 350
FVASRAFVQH VLENPKSQRL IEWVKDTYSP DEHLWATLQR APWMPGSVPY
360 370 380 390 400
HPKYHISDMT AIARLVKWQG HEGDVSMGAP YAPCSGIHQR AICIYGAGDL
410 420 430 440
HWILQNHHLL ANKFDPRVDD NVLQCLEEYL RHKAIYGTEL
Length:440
Mass (Da):50,953
Last modified:October 1, 2003 - v1
Checksum:i94D0EF6A08D9DA10
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72N → S in AAO22165 (PubMed:12525612).Curated1
Sequence conflicti263Y → S in AAP76324 (PubMed:14592928).Curated1
Sequence conflicti263Y → S in AAP76326 (PubMed:14592928).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF465338 Genomic DNA Translation: AAO22165.1
AY283763 mRNA Translation: AAP76324.1
AY283764 mRNA Translation: AAP76325.1
AY283765 mRNA Translation: AAP76326.1
AY283766 mRNA Translation: AAP76327.1
AY283767 Genomic DNA Translation: AAP76328.1
RefSeqiNP_991378.1, NM_205809.1
XP_005211713.1, XM_005211656.3
XP_010807543.1, XM_010809241.2
UniGeneiBt.51854

Genome annotation databases

EnsembliENSBTAT00000012427; ENSBTAP00000012427; ENSBTAG00000009443
GeneIDi404167
KEGGibta:404167

Similar proteinsi

Entry informationi

Entry nameiGCNT3_BOVIN
AccessioniPrimary (citable) accession number: Q7YQE1
Secondary accession number(s): Q7YQF8, Q866Z3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 1, 2003
Last modified: April 25, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health