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Q7YQE1

- GCNT3_BOVIN

UniProt

Q7YQE1 - GCNT3_BOVIN

Protein

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3

Gene

GCNT3

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development By similarity.By similarity

    Catalytic activityi

    UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R.
    UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.

    Pathwayi

    GO - Molecular functioni

    1. acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity Source: Ensembl
    2. beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC
    3. N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. immunoglobulin production in mucosal tissue Source: Ensembl
    2. intestinal absorption Source: Ensembl
    3. kidney morphogenesis Source: Ensembl
    4. protein glycosylation Source: UniProtKB-UniPathway
    5. tissue morphogenesis Source: Ensembl

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    ReactomeiREACT_208023. O-linked glycosylation of mucins.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT14. Glycosyltransferase Family 14.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 (EC:2.4.1.102, EC:2.4.1.150)
    Alternative name(s):
    C2GnT-mucin type
    Mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase
    bC2GnT-M
    Short name:
    C2GnT-M
    Gene namesi
    Name:GCNT3
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 10

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 440440Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3PRO_0000288543Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi72 – 721N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi73 ↔ 2301 Publication
    Glycosylationi108 – 1081N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi164 ↔ 3841 Publication
    Disulfide bondi185 ↔ 2121 Publication
    Disulfide bondi393 ↔ 4251 Publication

    Post-translational modificationi

    N-glycosylated.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ7YQE1.

    Expressioni

    Tissue specificityi

    Primarily expressed in mucus-secreting tissues.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7YQE1.
    SMRiQ7YQE1. Positions 70-435.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1212CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini31 – 440410LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei13 – 3018Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 14 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG253565.
    GeneTreeiENSGT00750000117608.
    HOGENOMiHOG000293251.
    HOVERGENiHBG051711.
    InParanoidiQ7YQE1.
    KOiK09662.
    OMAiPCSGIHQ.
    OrthoDBiEOG74N5HW.
    TreeFamiTF315534.

    Family and domain databases

    InterProiIPR003406. Glyco_trans_14.
    [Graphical view]
    PfamiPF02485. Branch. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7YQE1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKMTGWKKKL CRGHHLWALG CYMLLAVVAL RLSLRLKCDV DSLDLESRDF    50
    QSQRCRDVLY KNLKLPAKRS INCSGITRGD QEAVVQALLD NLEVKKKRLP 100
    FTDTYYLNIT RDCEQFKAQR KFIQFPLSKE ELDFPIAYSM VVHEKIENFE 150
    RLLRAVYAPQ NIYCVHVDVK SPETFKEAVK AIISCFPNVF MASKLVPVVY 200
    ASWSRVQADL NCMEDLLQSS VPWKYLLNTC GTDFPIKTNA EMVLALKMLN 250
    GKNSMESEIP SEYKKNRWKY RYEVTDRLYL TSKMKDPPPD NLPMFTGNAY 300
    FVASRAFVQH VLENPKSQRL IEWVKDTYSP DEHLWATLQR APWMPGSVPY 350
    HPKYHISDMT AIARLVKWQG HEGDVSMGAP YAPCSGIHQR AICIYGAGDL 400
    HWILQNHHLL ANKFDPRVDD NVLQCLEEYL RHKAIYGTEL 440
    Length:440
    Mass (Da):50,953
    Last modified:October 1, 2003 - v1
    Checksum:i94D0EF6A08D9DA10
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti72 – 721N → S in AAO22165. (PubMed:12525612)Curated
    Sequence conflicti263 – 2631Y → S in AAP76324. (PubMed:14592928)Curated
    Sequence conflicti263 – 2631Y → S in AAP76326. (PubMed:14592928)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF465338 Genomic DNA. Translation: AAO22165.1.
    AY283763 mRNA. Translation: AAP76324.1.
    AY283764 mRNA. Translation: AAP76325.1.
    AY283765 mRNA. Translation: AAP76326.1.
    AY283766 mRNA. Translation: AAP76327.1.
    AY283767 Genomic DNA. Translation: AAP76328.1.
    RefSeqiNP_991378.1. NM_205809.1.
    XP_005211713.1. XM_005211656.1.
    UniGeneiBt.51854.

    Genome annotation databases

    EnsembliENSBTAT00000012427; ENSBTAP00000012427; ENSBTAG00000009443.
    GeneIDi404167.
    KEGGibta:404167.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF465338 Genomic DNA. Translation: AAO22165.1 .
    AY283763 mRNA. Translation: AAP76324.1 .
    AY283764 mRNA. Translation: AAP76325.1 .
    AY283765 mRNA. Translation: AAP76326.1 .
    AY283766 mRNA. Translation: AAP76327.1 .
    AY283767 Genomic DNA. Translation: AAP76328.1 .
    RefSeqi NP_991378.1. NM_205809.1.
    XP_005211713.1. XM_005211656.1.
    UniGenei Bt.51854.

    3D structure databases

    ProteinModelPortali Q7YQE1.
    SMRi Q7YQE1. Positions 70-435.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT14. Glycosyltransferase Family 14.

    Proteomic databases

    PRIDEi Q7YQE1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000012427 ; ENSBTAP00000012427 ; ENSBTAG00000009443 .
    GeneIDi 404167.
    KEGGi bta:404167.

    Organism-specific databases

    CTDi 9245.

    Phylogenomic databases

    eggNOGi NOG253565.
    GeneTreei ENSGT00750000117608.
    HOGENOMi HOG000293251.
    HOVERGENi HBG051711.
    InParanoidi Q7YQE1.
    KOi K09662.
    OMAi PCSGIHQ.
    OrthoDBi EOG74N5HW.
    TreeFami TF315534.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_208023. O-linked glycosylation of mucins.

    Miscellaneous databases

    NextBioi 20817599.

    Family and domain databases

    InterProi IPR003406. Glyco_trans_14.
    [Graphical view ]
    Pfami PF02485. Branch. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The core 2 beta-1,6-N-acetylglucosaminyltransferase-mucin encoded by bovine herpesvirus 4 was acquired from an ancestor of the African buffalo."
      Markine-Goriaynoff N., Georgin J.-P., Goltz M., Zimmermann W., Broll H., Wamwayi H.M., Pastoret P.-P., Sharp P.M., Vanderplasschen A.
      J. Virol. 77:1784-1792(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Mucin biosynthesis: bovine C2GnT-M gene, tissue-specific expression, and herpes virus-4 homologue."
      Choi K.H., Osorio F.A., Cheng P.-W.
      Am. J. Respir. Cell Mol. Biol. 30:710-719(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Tissue: Colon and Tracheal epithelium.
    3. "Identification of disulfide bonds among the nine core 2 N-acetylglucosaminyltransferase-M cysteines conserved in the mucin beta6-N-acetylglucosaminyltransferase family."
      Singh J., Khan G.A., Kinarsky L., Cheng H., Wilken J., Choi K.H., Bedows E., Sherman S., Cheng P.-W.
      J. Biol. Chem. 279:38969-38977(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE, DISULFIDE BONDS, GLYCOSYLATION.

    Entry informationi

    Entry nameiGCNT3_BOVIN
    AccessioniPrimary (citable) accession number: Q7YQE1
    Secondary accession number(s): Q7YQF8, Q866Z3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3