Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q7YQE1 (GCNT3_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3

EC=2.4.1.102
EC=2.4.1.150
Alternative name(s):
C2GnT-mucin type
Mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase
bC2GnT-M
Short name=C2GnT-M
Gene names
Name:GCNT3
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length440 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development By similarity.

Catalytic activity

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R.

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein By similarity.

Tissue specificity

Primarily expressed in mucus-secreting tissues.

Post-translational modification

N-glycosylated Probable. Ref.3

Sequence similarities

Belongs to the glycosyltransferase 14 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 440440Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3
PRO_0000288543

Regions

Topological domain1 – 1212Cytoplasmic Potential
Transmembrane13 – 3018Helical; Signal-anchor for type II membrane protein; Potential
Topological domain31 – 440410Lumenal Potential

Amino acid modifications

Glycosylation721N-linked (GlcNAc...) Potential
Glycosylation1081N-linked (GlcNAc...) Potential
Disulfide bond73 ↔ 230 Ref.3
Disulfide bond164 ↔ 384 Ref.3
Disulfide bond185 ↔ 212 Ref.3
Disulfide bond393 ↔ 425 Ref.3

Experimental info

Sequence conflict721N → S in AAO22165. Ref.1
Sequence conflict2631Y → S in AAP76324. Ref.2
Sequence conflict2631Y → S in AAP76326. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q7YQE1 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 94D0EF6A08D9DA10

FASTA44050,953
        10         20         30         40         50         60 
MKMTGWKKKL CRGHHLWALG CYMLLAVVAL RLSLRLKCDV DSLDLESRDF QSQRCRDVLY 

        70         80         90        100        110        120 
KNLKLPAKRS INCSGITRGD QEAVVQALLD NLEVKKKRLP FTDTYYLNIT RDCEQFKAQR 

       130        140        150        160        170        180 
KFIQFPLSKE ELDFPIAYSM VVHEKIENFE RLLRAVYAPQ NIYCVHVDVK SPETFKEAVK 

       190        200        210        220        230        240 
AIISCFPNVF MASKLVPVVY ASWSRVQADL NCMEDLLQSS VPWKYLLNTC GTDFPIKTNA 

       250        260        270        280        290        300 
EMVLALKMLN GKNSMESEIP SEYKKNRWKY RYEVTDRLYL TSKMKDPPPD NLPMFTGNAY 

       310        320        330        340        350        360 
FVASRAFVQH VLENPKSQRL IEWVKDTYSP DEHLWATLQR APWMPGSVPY HPKYHISDMT 

       370        380        390        400        410        420 
AIARLVKWQG HEGDVSMGAP YAPCSGIHQR AICIYGAGDL HWILQNHHLL ANKFDPRVDD 

       430        440 
NVLQCLEEYL RHKAIYGTEL 

« Hide

References

[1]"The core 2 beta-1,6-N-acetylglucosaminyltransferase-mucin encoded by bovine herpesvirus 4 was acquired from an ancestor of the African buffalo."
Markine-Goriaynoff N., Georgin J.-P., Goltz M., Zimmermann W., Broll H., Wamwayi H.M., Pastoret P.-P., Sharp P.M., Vanderplasschen A.
J. Virol. 77:1784-1792(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Mucin biosynthesis: bovine C2GnT-M gene, tissue-specific expression, and herpes virus-4 homologue."
Choi K.H., Osorio F.A., Cheng P.-W.
Am. J. Respir. Cell Mol. Biol. 30:710-719(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Tissue: Colon and Tracheal epithelium.
[3]"Identification of disulfide bonds among the nine core 2 N-acetylglucosaminyltransferase-M cysteines conserved in the mucin beta6-N-acetylglucosaminyltransferase family."
Singh J., Khan G.A., Kinarsky L., Cheng H., Wilken J., Choi K.H., Bedows E., Sherman S., Cheng P.-W.
J. Biol. Chem. 279:38969-38977(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE, DISULFIDE BONDS, GLYCOSYLATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF465338 Genomic DNA. Translation: AAO22165.1.
AY283763 mRNA. Translation: AAP76324.1.
AY283764 mRNA. Translation: AAP76325.1.
AY283765 mRNA. Translation: AAP76326.1.
AY283766 mRNA. Translation: AAP76327.1.
AY283767 Genomic DNA. Translation: AAP76328.1.
RefSeqNP_991378.1. NM_205809.1.
XP_005211713.1. XM_005211656.1.
UniGeneBt.51854.

3D structure databases

ProteinModelPortalQ7YQE1.
SMRQ7YQE1. Positions 70-435.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGT14. Glycosyltransferase Family 14.

Proteomic databases

PRIDEQ7YQE1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000012427; ENSBTAP00000012427; ENSBTAG00000009443.
GeneID404167.
KEGGbta:404167.

Organism-specific databases

CTD9245.

Phylogenomic databases

eggNOGNOG253565.
GeneTreeENSGT00750000117608.
HOGENOMHOG000293251.
HOVERGENHBG051711.
InParanoidQ7YQE1.
KOK09662.
OMAPCSGIHQ.
OrthoDBEOG74N5HW.
TreeFamTF315534.

Enzyme and pathway databases

UniPathwayUPA00378.

Family and domain databases

InterProIPR003406. Glyco_trans_14.
[Graphical view]
PfamPF02485. Branch. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20817599.

Entry information

Entry nameGCNT3_BOVIN
AccessionPrimary (citable) accession number: Q7YQE1
Secondary accession number(s): Q7YQF8, Q866Z3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 1, 2003
Last modified: April 16, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways