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Protein

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3

Gene

GCNT3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development (By similarity).By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R.
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.

Pathwayi

GO - Molecular functioni

  1. acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity Source: Ensembl
  2. beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC
  3. N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. immunoglobulin production in mucosal tissue Source: Ensembl
  2. intestinal absorption Source: Ensembl
  3. kidney morphogenesis Source: Ensembl
  4. protein glycosylation Source: UniProtKB-UniPathway
  5. tissue morphogenesis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_208023. O-linked glycosylation of mucins.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT14. Glycosyltransferase Family 14.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 (EC:2.4.1.102, EC:2.4.1.150)
Alternative name(s):
C2GnT-mucin type
Mucus-type core 2 beta-1,6-N-acetylglucosaminyltransferase
bC2GnT-M
Short name:
C2GnT-M
Gene namesi
Name:GCNT3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 10

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei13 – 3018Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini31 – 440410LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. Golgi membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 440440Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3PRO_0000288543Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi72 – 721N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi73 ↔ 2301 Publication
Glycosylationi108 – 1081N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi164 ↔ 3841 Publication
Disulfide bondi185 ↔ 2121 Publication
Disulfide bondi393 ↔ 4251 Publication

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ7YQE1.

Expressioni

Tissue specificityi

Primarily expressed in mucus-secreting tissues.

Structurei

3D structure databases

ProteinModelPortaliQ7YQE1.
SMRiQ7YQE1. Positions 70-435.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 14 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG253565.
GeneTreeiENSGT00760000119183.
HOGENOMiHOG000293251.
HOVERGENiHBG051711.
InParanoidiQ7YQE1.
KOiK09662.
OMAiQQLIEWV.
OrthoDBiEOG74N5HW.
TreeFamiTF315534.

Family and domain databases

InterProiIPR003406. Glyco_trans_14.
[Graphical view]
PfamiPF02485. Branch. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7YQE1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMTGWKKKL CRGHHLWALG CYMLLAVVAL RLSLRLKCDV DSLDLESRDF
60 70 80 90 100
QSQRCRDVLY KNLKLPAKRS INCSGITRGD QEAVVQALLD NLEVKKKRLP
110 120 130 140 150
FTDTYYLNIT RDCEQFKAQR KFIQFPLSKE ELDFPIAYSM VVHEKIENFE
160 170 180 190 200
RLLRAVYAPQ NIYCVHVDVK SPETFKEAVK AIISCFPNVF MASKLVPVVY
210 220 230 240 250
ASWSRVQADL NCMEDLLQSS VPWKYLLNTC GTDFPIKTNA EMVLALKMLN
260 270 280 290 300
GKNSMESEIP SEYKKNRWKY RYEVTDRLYL TSKMKDPPPD NLPMFTGNAY
310 320 330 340 350
FVASRAFVQH VLENPKSQRL IEWVKDTYSP DEHLWATLQR APWMPGSVPY
360 370 380 390 400
HPKYHISDMT AIARLVKWQG HEGDVSMGAP YAPCSGIHQR AICIYGAGDL
410 420 430 440
HWILQNHHLL ANKFDPRVDD NVLQCLEEYL RHKAIYGTEL
Length:440
Mass (Da):50,953
Last modified:October 1, 2003 - v1
Checksum:i94D0EF6A08D9DA10
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti72 – 721N → S in AAO22165 (PubMed:12525612).Curated
Sequence conflicti263 – 2631Y → S in AAP76324 (PubMed:14592928).Curated
Sequence conflicti263 – 2631Y → S in AAP76326 (PubMed:14592928).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF465338 Genomic DNA. Translation: AAO22165.1.
AY283763 mRNA. Translation: AAP76324.1.
AY283764 mRNA. Translation: AAP76325.1.
AY283765 mRNA. Translation: AAP76326.1.
AY283766 mRNA. Translation: AAP76327.1.
AY283767 Genomic DNA. Translation: AAP76328.1.
RefSeqiNP_991378.1. NM_205809.1.
XP_005211713.1. XM_005211656.2.
XP_010807543.1. XM_010809241.1.
UniGeneiBt.51854.

Genome annotation databases

EnsembliENSBTAT00000012427; ENSBTAP00000012427; ENSBTAG00000009443.
GeneIDi404167.
KEGGibta:404167.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF465338 Genomic DNA. Translation: AAO22165.1.
AY283763 mRNA. Translation: AAP76324.1.
AY283764 mRNA. Translation: AAP76325.1.
AY283765 mRNA. Translation: AAP76326.1.
AY283766 mRNA. Translation: AAP76327.1.
AY283767 Genomic DNA. Translation: AAP76328.1.
RefSeqiNP_991378.1. NM_205809.1.
XP_005211713.1. XM_005211656.2.
XP_010807543.1. XM_010809241.1.
UniGeneiBt.51854.

3D structure databases

ProteinModelPortaliQ7YQE1.
SMRiQ7YQE1. Positions 70-435.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT14. Glycosyltransferase Family 14.

Proteomic databases

PRIDEiQ7YQE1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000012427; ENSBTAP00000012427; ENSBTAG00000009443.
GeneIDi404167.
KEGGibta:404167.

Organism-specific databases

CTDi9245.

Phylogenomic databases

eggNOGiNOG253565.
GeneTreeiENSGT00760000119183.
HOGENOMiHOG000293251.
HOVERGENiHBG051711.
InParanoidiQ7YQE1.
KOiK09662.
OMAiQQLIEWV.
OrthoDBiEOG74N5HW.
TreeFamiTF315534.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_208023. O-linked glycosylation of mucins.

Miscellaneous databases

NextBioi20817599.

Family and domain databases

InterProiIPR003406. Glyco_trans_14.
[Graphical view]
PfamiPF02485. Branch. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The core 2 beta-1,6-N-acetylglucosaminyltransferase-mucin encoded by bovine herpesvirus 4 was acquired from an ancestor of the African buffalo."
    Markine-Goriaynoff N., Georgin J.-P., Goltz M., Zimmermann W., Broll H., Wamwayi H.M., Pastoret P.-P., Sharp P.M., Vanderplasschen A.
    J. Virol. 77:1784-1792(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Mucin biosynthesis: bovine C2GnT-M gene, tissue-specific expression, and herpes virus-4 homologue."
    Choi K.H., Osorio F.A., Cheng P.-W.
    Am. J. Respir. Cell Mol. Biol. 30:710-719(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Tissue: Colon and Tracheal epithelium.
  3. "Identification of disulfide bonds among the nine core 2 N-acetylglucosaminyltransferase-M cysteines conserved in the mucin beta6-N-acetylglucosaminyltransferase family."
    Singh J., Khan G.A., Kinarsky L., Cheng H., Wilken J., Choi K.H., Bedows E., Sherman S., Cheng P.-W.
    J. Biol. Chem. 279:38969-38977(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, DISULFIDE BONDS, GLYCOSYLATION.

Entry informationi

Entry nameiGCNT3_BOVIN
AccessioniPrimary (citable) accession number: Q7YQE1
Secondary accession number(s): Q7YQF8, Q866Z3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 1, 2003
Last modified: March 4, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.