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Q7Y232 (U73B4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UDP-glycosyltransferase 73B4

EC=2.4.1.-
Alternative name(s):
Flavonol 3-O-glucosyltransferase UGT73B4
EC=2.4.1.91
Gene names
Name:UGT73B4
Ordered Locus Names:At2g15490
ORF Names:F9O13.4
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length484 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Possesses quercetin 3-O-glucosyltransferase and low 7-O-glucosyltransferase activities in vitro. Also active in vitro on benzoates and benzoate derivatives. Can detoxify the explosive 2,4,6-trinitrotoluene in plant by forming O- or C-glucose conjugates. Ref.6 Ref.7 Ref.9

Catalytic activity

UDP-glucose + a flavonol = UDP + a flavonol 3-O-D-glucoside.

Tissue specificity

Specifically expressed in roots. Ref.8

Induction

Induced by pathogen infection and salicylic acid. Ref.8

Miscellaneous

Plants overexpressing UGT73B4 show enhanced root growth in seedlings grown in presence of 2,4,6-trinitrotoluene.

Sequence similarities

Belongs to the UDP-glycosyltransferase family.

Sequence caution

The sequence AAD17393.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q7Y232-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q7Y232-2)

The sequence of this isoform differs from the canonical sequence as follows:
     189-402: Missing.
Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 484484UDP-glycosyltransferase 73B4
PRO_0000409079

Regions

Region356 – 3583UDP-glucose binding By similarity
Region373 – 3819UDP-glucose binding By similarity
Region395 – 3984UDP-glucose binding By similarity

Sites

Binding site2941UDP-glucose By similarity

Natural variations

Alternative sequence189 – 402214Missing in isoform 2.
VSP_041227

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 41C07469E63747D7

FASTA48453,961
        10         20         30         40         50         60 
MNREQIHILF FPFMAHGHMI PLLDMAKLFA RRGAKSTLLT TPINAKILEK PIEAFKVQNP 

        70         80         90        100        110        120 
DLEIGIKILN FPCVELGLPE GCENRDFINS YQKSDSFDLF LKFLFSTKYM KQQLESFIET 

       130        140        150        160        170        180 
TKPSALVADM FFPWATESAE KIGVPRLVFH GTSSFALCCS YNMRIHKPHK KVASSSTPFV 

       190        200        210        220        230        240 
IPGLPGDIVI TEDQANVTNE ETPFGKFWKE VRESETSSFG VLVNSFYELE SSYADFYRSF 

       250        260        270        280        290        300 
VAKKAWHIGP LSLSNRGIAE KAGRGKKANI DEQECLKWLD SKTPGSVVYL SFGSGTGLPN 

       310        320        330        340        350        360 
EQLLEIAFGL EGSGQNFIWV VSKNENQVGT GENEDWLPKG FEERNKGKGL IIRGWAPQVL 

       370        380        390        400        410        420 
ILDHKAIGGF VTHCGWNSTL EGIAAGLPMV TWPMGAEQFY NEKLLTKVLR IGVNVGATEL 

       430        440        450        460        470        480 
VKKGKLISRA QVEKAVREVI GGEKAEERRL RAKELGEMAK AAVEEGGSSY NDVNKFMEEL 


NGRK 

« Hide

Isoform 2 [UniParc].

Checksum: 5CEF0CE3B3D1A3BE
Show »

FASTA27030,278

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[4]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[5]"Phylogenetic analysis of the UDP-glycosyltransferase multigene family of Arabidopsis thaliana."
Li Y., Baldauf S., Lim E.K., Bowles D.J.
J. Biol. Chem. 276:4338-4343(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
[6]"The activity of Arabidopsis glycosyltransferases toward salicylic acid, 4-hydroxybenzoic acid, and other benzoates."
Lim E.K., Doucet C.J., Li Y., Elias L., Worrall D., Spencer S.P., Ross J., Bowles D.J.
J. Biol. Chem. 277:586-592(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Arabidopsis glycosyltransferases as biocatalysts in fermentation for regioselective synthesis of diverse quercetin glucosides."
Lim E.K., Ashford D.A., Hou B., Jackson R.G., Bowles D.J.
Biotechnol. Bioeng. 87:623-631(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Pathogen-responsive expression of glycosyltransferase genes UGT73B3 and UGT73B5 is necessary for resistance to Pseudomonas syringae pv tomato in Arabidopsis."
Langlois-Meurinne M., Gachon C.M., Saindrenan P.
Plant Physiol. 139:1890-1901(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION BY PATHOGEN AND SALICYLIC ACID.
[9]"Detoxification of the explosive 2,4,6-trinitrotoluene in Arabidopsis: discovery of bifunctional O- and C-glucosyltransferases."
Gandia-Herrero F., Lorenz A., Larson T., Graham I.A., Bowles D.J., Rylott E.L., Bruce N.C.
Plant J. 56:963-974(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC006248 Genomic DNA. Translation: AAD17393.1. Different initiation.
CP002685 Genomic DNA. Translation: AEC06407.1.
CP002685 Genomic DNA. Translation: AEC06408.1.
BT008319 mRNA. Translation: AAP37678.1.
AK227684 mRNA. Translation: BAE99671.1.
PIRF84529.
RefSeqNP_179151.2. NM_127109.3. [Q7Y232-1]
NP_973469.1. NM_201740.1. [Q7Y232-2]
UniGeneAt.40404.

3D structure databases

ProteinModelPortalQ7Y232.
SMRQ7Y232. Positions 8-482.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT2G15490.1-P.

Protein family/group databases

CAZyGT1. Glycosyltransferase Family 1.

Proteomic databases

PaxDbQ7Y232.
PRIDEQ7Y232.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G15490.1; AT2G15490.1; AT2G15490. [Q7Y232-1]
GeneID816041.
KEGGath:AT2G15490.

Organism-specific databases

TAIRAT2G15490.

Phylogenomic databases

eggNOGNOG263906.
HOGENOMHOG000237565.
InParanoidQ7Y232.
OMAENRDFIN.
PhylomeDBQ7Y232.

Enzyme and pathway databases

BioCycARA:GQT-2588-MONOMER.

Gene expression databases

ArrayExpressQ7Y232.
GenevestigatorQ7Y232.

Family and domain databases

InterProIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERPTHR11926. PTHR11926. 1 hit.
PfamPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEPS00375. UDPGT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameU73B4_ARATH
AccessionPrimary (citable) accession number: Q7Y232
Secondary accession number(s): Q3EBZ8, Q9ZQG3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: October 1, 2003
Last modified: May 14, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names