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Q7Y232

- U73B4_ARATH

UniProt

Q7Y232 - U73B4_ARATH

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Protein
UDP-glycosyltransferase 73B4
Gene
UGT73B4, At2g15490, F9O13.4
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Possesses quercetin 3-O-glucosyltransferase and low 7-O-glucosyltransferase activities in vitro. Also active in vitro on benzoates and benzoate derivatives. Can detoxify the explosive 2,4,6-trinitrotoluene in plant by forming O- or C-glucose conjugates.3 Publications

Catalytic activityi

UDP-glucose + a flavonol = UDP + a flavonol 3-O-D-glucoside.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei294 – 2941UDP-glucose By similarity

GO - Molecular functioni

  1. UDP-glucosyltransferase activity Source: TAIR
  2. flavonol 3-O-glucosyltransferase activity Source: UniProtKB-EC
  3. quercetin 3-O-glucosyltransferase activity Source: TAIR
  4. quercetin 7-O-glucosyltransferase activity Source: TAIR
Complete GO annotation...

GO - Biological processi

  1. response to other organism Source: TAIR
  2. response to toxic substance Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Detoxification

Enzyme and pathway databases

BioCyciARA:GQT-2588-MONOMER.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glycosyltransferase 73B4 (EC:2.4.1.-)
Alternative name(s):
Flavonol 3-O-glucosyltransferase UGT73B4 (EC:2.4.1.91)
Gene namesi
Name:UGT73B4
Ordered Locus Names:At2g15490
ORF Names:F9O13.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G15490.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 484484UDP-glycosyltransferase 73B4
PRO_0000409079Add
BLAST

Proteomic databases

PaxDbiQ7Y232.
PRIDEiQ7Y232.

Expressioni

Tissue specificityi

Specifically expressed in roots.1 Publication

Inductioni

Induced by pathogen infection and salicylic acid.1 Publication

Gene expression databases

ArrayExpressiQ7Y232.
GenevestigatoriQ7Y232.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G15490.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ7Y232.
SMRiQ7Y232. Positions 8-482.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni356 – 3583UDP-glucose binding By similarity
Regioni373 – 3819UDP-glucose binding By similarity
Regioni395 – 3984UDP-glucose binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG263906.
HOGENOMiHOG000237565.
InParanoidiQ7Y232.
OMAiENRDFIN.
PhylomeDBiQ7Y232.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q7Y232-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MNREQIHILF FPFMAHGHMI PLLDMAKLFA RRGAKSTLLT TPINAKILEK    50
PIEAFKVQNP DLEIGIKILN FPCVELGLPE GCENRDFINS YQKSDSFDLF 100
LKFLFSTKYM KQQLESFIET TKPSALVADM FFPWATESAE KIGVPRLVFH 150
GTSSFALCCS YNMRIHKPHK KVASSSTPFV IPGLPGDIVI TEDQANVTNE 200
ETPFGKFWKE VRESETSSFG VLVNSFYELE SSYADFYRSF VAKKAWHIGP 250
LSLSNRGIAE KAGRGKKANI DEQECLKWLD SKTPGSVVYL SFGSGTGLPN 300
EQLLEIAFGL EGSGQNFIWV VSKNENQVGT GENEDWLPKG FEERNKGKGL 350
IIRGWAPQVL ILDHKAIGGF VTHCGWNSTL EGIAAGLPMV TWPMGAEQFY 400
NEKLLTKVLR IGVNVGATEL VKKGKLISRA QVEKAVREVI GGEKAEERRL 450
RAKELGEMAK AAVEEGGSSY NDVNKFMEEL NGRK 484
Length:484
Mass (Da):53,961
Last modified:October 1, 2003 - v1
Checksum:i41C07469E63747D7
GO
Isoform 2 (identifier: Q7Y232-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     189-402: Missing.

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:270
Mass (Da):30,278
Checksum:i5CEF0CE3B3D1A3BE
GO

Sequence cautioni

The sequence AAD17393.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei189 – 402214Missing in isoform 2.
VSP_041227Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC006248 Genomic DNA. Translation: AAD17393.1. Different initiation.
CP002685 Genomic DNA. Translation: AEC06407.1.
CP002685 Genomic DNA. Translation: AEC06408.1.
BT008319 mRNA. Translation: AAP37678.1.
AK227684 mRNA. Translation: BAE99671.1.
PIRiF84529.
RefSeqiNP_179151.2. NM_127109.3. [Q7Y232-1]
NP_973469.1. NM_201740.1. [Q7Y232-2]
UniGeneiAt.40404.

Genome annotation databases

EnsemblPlantsiAT2G15490.1; AT2G15490.1; AT2G15490. [Q7Y232-1]
GeneIDi816041.
KEGGiath:AT2G15490.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC006248 Genomic DNA. Translation: AAD17393.1 . Different initiation.
CP002685 Genomic DNA. Translation: AEC06407.1 .
CP002685 Genomic DNA. Translation: AEC06408.1 .
BT008319 mRNA. Translation: AAP37678.1 .
AK227684 mRNA. Translation: BAE99671.1 .
PIRi F84529.
RefSeqi NP_179151.2. NM_127109.3. [Q7Y232-1 ]
NP_973469.1. NM_201740.1. [Q7Y232-2 ]
UniGenei At.40404.

3D structure databases

ProteinModelPortali Q7Y232.
SMRi Q7Y232. Positions 8-482.
ModBasei Search...

Protein-protein interaction databases

STRINGi 3702.AT2G15490.1-P.

Protein family/group databases

CAZyi GT1. Glycosyltransferase Family 1.

Proteomic databases

PaxDbi Q7Y232.
PRIDEi Q7Y232.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G15490.1 ; AT2G15490.1 ; AT2G15490 . [Q7Y232-1 ]
GeneIDi 816041.
KEGGi ath:AT2G15490.

Organism-specific databases

TAIRi AT2G15490.

Phylogenomic databases

eggNOGi NOG263906.
HOGENOMi HOG000237565.
InParanoidi Q7Y232.
OMAi ENRDFIN.
PhylomeDBi Q7Y232.

Enzyme and pathway databases

BioCyci ARA:GQT-2588-MONOMER.

Gene expression databases

ArrayExpressi Q7Y232.
Genevestigatori Q7Y232.

Family and domain databases

InterProi IPR002213. UDP_glucos_trans.
[Graphical view ]
PANTHERi PTHR11926. PTHR11926. 1 hit.
Pfami PF00201. UDPGT. 1 hit.
[Graphical view ]
PROSITEi PS00375. UDPGT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Phylogenetic analysis of the UDP-glycosyltransferase multigene family of Arabidopsis thaliana."
    Li Y., Baldauf S., Lim E.K., Bowles D.J.
    J. Biol. Chem. 276:4338-4343(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  6. "The activity of Arabidopsis glycosyltransferases toward salicylic acid, 4-hydroxybenzoic acid, and other benzoates."
    Lim E.K., Doucet C.J., Li Y., Elias L., Worrall D., Spencer S.P., Ross J., Bowles D.J.
    J. Biol. Chem. 277:586-592(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Arabidopsis glycosyltransferases as biocatalysts in fermentation for regioselective synthesis of diverse quercetin glucosides."
    Lim E.K., Ashford D.A., Hou B., Jackson R.G., Bowles D.J.
    Biotechnol. Bioeng. 87:623-631(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Pathogen-responsive expression of glycosyltransferase genes UGT73B3 and UGT73B5 is necessary for resistance to Pseudomonas syringae pv tomato in Arabidopsis."
    Langlois-Meurinne M., Gachon C.M., Saindrenan P.
    Plant Physiol. 139:1890-1901(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION BY PATHOGEN AND SALICYLIC ACID.
  9. "Detoxification of the explosive 2,4,6-trinitrotoluene in Arabidopsis: discovery of bifunctional O- and C-glucosyltransferases."
    Gandia-Herrero F., Lorenz A., Larson T., Graham I.A., Bowles D.J., Rylott E.L., Bruce N.C.
    Plant J. 56:963-974(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiU73B4_ARATH
AccessioniPrimary (citable) accession number: Q7Y232
Secondary accession number(s): Q3EBZ8, Q9ZQG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: October 1, 2003
Last modified: May 14, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Plants overexpressing UGT73B4 show enhanced root growth in seedlings grown in presence of 2,4,6-trinitrotoluene.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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