Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q7Y232

- U73B4_ARATH

UniProt

Q7Y232 - U73B4_ARATH

Protein

UDP-glycosyltransferase 73B4

Gene

UGT73B4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Possesses quercetin 3-O-glucosyltransferase and low 7-O-glucosyltransferase activities in vitro. Also active in vitro on benzoates and benzoate derivatives. Can detoxify the explosive 2,4,6-trinitrotoluene in plant by forming O- or C-glucose conjugates.3 Publications

    Catalytic activityi

    UDP-glucose + a flavonol = UDP + a flavonol 3-O-D-glucoside.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei294 – 2941UDP-glucoseBy similarity

    GO - Molecular functioni

    1. flavonol 3-O-glucosyltransferase activity Source: UniProtKB-EC
    2. quercetin 3-O-glucosyltransferase activity Source: TAIR
    3. quercetin 7-O-glucosyltransferase activity Source: TAIR
    4. UDP-glucosyltransferase activity Source: TAIR

    GO - Biological processi

    1. response to other organism Source: TAIR
    2. response to toxic substance Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Detoxification

    Enzyme and pathway databases

    BioCyciARA:GQT-2588-MONOMER.

    Protein family/group databases

    CAZyiGT1. Glycosyltransferase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-glycosyltransferase 73B4 (EC:2.4.1.-)
    Alternative name(s):
    Flavonol 3-O-glucosyltransferase UGT73B4 (EC:2.4.1.91)
    Gene namesi
    Name:UGT73B4
    Ordered Locus Names:At2g15490
    ORF Names:F9O13.4
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G15490.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 484484UDP-glycosyltransferase 73B4PRO_0000409079Add
    BLAST

    Proteomic databases

    PaxDbiQ7Y232.
    PRIDEiQ7Y232.

    Expressioni

    Tissue specificityi

    Specifically expressed in roots.1 Publication

    Inductioni

    Induced by pathogen infection and salicylic acid.1 Publication

    Gene expression databases

    ArrayExpressiQ7Y232.
    GenevestigatoriQ7Y232.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G15490.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7Y232.
    SMRiQ7Y232. Positions 8-482.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni356 – 3583UDP-glucose bindingBy similarity
    Regioni373 – 3819UDP-glucose bindingBy similarity
    Regioni395 – 3984UDP-glucose bindingBy similarity

    Sequence similaritiesi

    Belongs to the UDP-glycosyltransferase family.Curated

    Phylogenomic databases

    eggNOGiNOG263906.
    HOGENOMiHOG000237565.
    InParanoidiQ7Y232.
    OMAiENRDFIN.
    PhylomeDBiQ7Y232.

    Family and domain databases

    InterProiIPR002213. UDP_glucos_trans.
    [Graphical view]
    PANTHERiPTHR11926. PTHR11926. 1 hit.
    PfamiPF00201. UDPGT. 1 hit.
    [Graphical view]
    PROSITEiPS00375. UDPGT. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q7Y232-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MNREQIHILF FPFMAHGHMI PLLDMAKLFA RRGAKSTLLT TPINAKILEK    50
    PIEAFKVQNP DLEIGIKILN FPCVELGLPE GCENRDFINS YQKSDSFDLF 100
    LKFLFSTKYM KQQLESFIET TKPSALVADM FFPWATESAE KIGVPRLVFH 150
    GTSSFALCCS YNMRIHKPHK KVASSSTPFV IPGLPGDIVI TEDQANVTNE 200
    ETPFGKFWKE VRESETSSFG VLVNSFYELE SSYADFYRSF VAKKAWHIGP 250
    LSLSNRGIAE KAGRGKKANI DEQECLKWLD SKTPGSVVYL SFGSGTGLPN 300
    EQLLEIAFGL EGSGQNFIWV VSKNENQVGT GENEDWLPKG FEERNKGKGL 350
    IIRGWAPQVL ILDHKAIGGF VTHCGWNSTL EGIAAGLPMV TWPMGAEQFY 400
    NEKLLTKVLR IGVNVGATEL VKKGKLISRA QVEKAVREVI GGEKAEERRL 450
    RAKELGEMAK AAVEEGGSSY NDVNKFMEEL NGRK 484
    Length:484
    Mass (Da):53,961
    Last modified:October 1, 2003 - v1
    Checksum:i41C07469E63747D7
    GO
    Isoform 2 (identifier: Q7Y232-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         189-402: Missing.

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:270
    Mass (Da):30,278
    Checksum:i5CEF0CE3B3D1A3BE
    GO

    Sequence cautioni

    The sequence AAD17393.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei189 – 402214Missing in isoform 2. CuratedVSP_041227Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006248 Genomic DNA. Translation: AAD17393.1. Different initiation.
    CP002685 Genomic DNA. Translation: AEC06407.1.
    CP002685 Genomic DNA. Translation: AEC06408.1.
    BT008319 mRNA. Translation: AAP37678.1.
    AK227684 mRNA. Translation: BAE99671.1.
    PIRiF84529.
    RefSeqiNP_179151.2. NM_127109.3. [Q7Y232-1]
    NP_973469.1. NM_201740.1. [Q7Y232-2]
    UniGeneiAt.40404.

    Genome annotation databases

    EnsemblPlantsiAT2G15490.1; AT2G15490.1; AT2G15490. [Q7Y232-1]
    GeneIDi816041.
    KEGGiath:AT2G15490.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006248 Genomic DNA. Translation: AAD17393.1 . Different initiation.
    CP002685 Genomic DNA. Translation: AEC06407.1 .
    CP002685 Genomic DNA. Translation: AEC06408.1 .
    BT008319 mRNA. Translation: AAP37678.1 .
    AK227684 mRNA. Translation: BAE99671.1 .
    PIRi F84529.
    RefSeqi NP_179151.2. NM_127109.3. [Q7Y232-1 ]
    NP_973469.1. NM_201740.1. [Q7Y232-2 ]
    UniGenei At.40404.

    3D structure databases

    ProteinModelPortali Q7Y232.
    SMRi Q7Y232. Positions 8-482.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT2G15490.1-P.

    Protein family/group databases

    CAZyi GT1. Glycosyltransferase Family 1.

    Proteomic databases

    PaxDbi Q7Y232.
    PRIDEi Q7Y232.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G15490.1 ; AT2G15490.1 ; AT2G15490 . [Q7Y232-1 ]
    GeneIDi 816041.
    KEGGi ath:AT2G15490.

    Organism-specific databases

    TAIRi AT2G15490.

    Phylogenomic databases

    eggNOGi NOG263906.
    HOGENOMi HOG000237565.
    InParanoidi Q7Y232.
    OMAi ENRDFIN.
    PhylomeDBi Q7Y232.

    Enzyme and pathway databases

    BioCyci ARA:GQT-2588-MONOMER.

    Gene expression databases

    ArrayExpressi Q7Y232.
    Genevestigatori Q7Y232.

    Family and domain databases

    InterProi IPR002213. UDP_glucos_trans.
    [Graphical view ]
    PANTHERi PTHR11926. PTHR11926. 1 hit.
    Pfami PF00201. UDPGT. 1 hit.
    [Graphical view ]
    PROSITEi PS00375. UDPGT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    5. "Phylogenetic analysis of the UDP-glycosyltransferase multigene family of Arabidopsis thaliana."
      Li Y., Baldauf S., Lim E.K., Bowles D.J.
      J. Biol. Chem. 276:4338-4343(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY.
    6. "The activity of Arabidopsis glycosyltransferases toward salicylic acid, 4-hydroxybenzoic acid, and other benzoates."
      Lim E.K., Doucet C.J., Li Y., Elias L., Worrall D., Spencer S.P., Ross J., Bowles D.J.
      J. Biol. Chem. 277:586-592(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Arabidopsis glycosyltransferases as biocatalysts in fermentation for regioselective synthesis of diverse quercetin glucosides."
      Lim E.K., Ashford D.A., Hou B., Jackson R.G., Bowles D.J.
      Biotechnol. Bioeng. 87:623-631(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Pathogen-responsive expression of glycosyltransferase genes UGT73B3 and UGT73B5 is necessary for resistance to Pseudomonas syringae pv tomato in Arabidopsis."
      Langlois-Meurinne M., Gachon C.M., Saindrenan P.
      Plant Physiol. 139:1890-1901(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INDUCTION BY PATHOGEN AND SALICYLIC ACID.
    9. "Detoxification of the explosive 2,4,6-trinitrotoluene in Arabidopsis: discovery of bifunctional O- and C-glucosyltransferases."
      Gandia-Herrero F., Lorenz A., Larson T., Graham I.A., Bowles D.J., Rylott E.L., Bruce N.C.
      Plant J. 56:963-974(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiU73B4_ARATH
    AccessioniPrimary (citable) accession number: Q7Y232
    Secondary accession number(s): Q3EBZ8, Q9ZQG3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 31, 2011
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Plants overexpressing UGT73B4 show enhanced root growth in seedlings grown in presence of 2,4,6-trinitrotoluene.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3