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Protein

UDP-glycosyltransferase 73B4

Gene

UGT73B4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Possesses quercetin 3-O-glucosyltransferase and low 7-O-glucosyltransferase activities in vitro. Also active in vitro on benzoates and benzoate derivatives. Can detoxify the explosive 2,4,6-trinitrotoluene in plant by forming O- or C-glucose conjugates.3 Publications

Miscellaneous

Plants overexpressing UGT73B4 show enhanced root growth in seedlings grown in presence of 2,4,6-trinitrotoluene.

Catalytic activityi

UDP-glucose + a flavonol = UDP + a flavonol 3-O-D-glucoside.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei294UDP-glucoseBy similarity1

GO - Molecular functioni

GO - Biological processi

  • metabolic process Source: InterPro
  • response to other organism Source: TAIR
  • response to toxic substance Source: UniProtKB-KW

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processDetoxification

Protein family/group databases

CAZyiGT1 Glycosyltransferase Family 1

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glycosyltransferase 73B4 (EC:2.4.1.-)
Alternative name(s):
Flavonol 3-O-glucosyltransferase UGT73B4 (EC:2.4.1.91)
Gene namesi
Name:UGT73B4
Ordered Locus Names:At2g15490
ORF Names:F9O13.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G15490
TAIRilocus:2053669 AT2G15490

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004090791 – 484UDP-glycosyltransferase 73B4Add BLAST484

Proteomic databases

PaxDbiQ7Y232
PRIDEiQ7Y232

PTM databases

iPTMnetiQ7Y232

Expressioni

Tissue specificityi

Specifically expressed in roots.1 Publication

Inductioni

Induced by pathogen infection and salicylic acid.1 Publication

Gene expression databases

ExpressionAtlasiQ7Y232 baseline and differential
GenevisibleiQ7Y232 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G15490.1

Structurei

3D structure databases

ProteinModelPortaliQ7Y232
SMRiQ7Y232
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni356 – 358UDP-glucose bindingBy similarity3
Regioni373 – 381UDP-glucose bindingBy similarity9
Regioni395 – 398UDP-glucose bindingBy similarity4

Sequence similaritiesi

Belongs to the UDP-glycosyltransferase family.Curated

Phylogenomic databases

eggNOGiKOG1192 Eukaryota
COG1819 LUCA
HOGENOMiHOG000237565
InParanoidiQ7Y232
OMAiLPGNIVM
OrthoDBiEOG093608LC
PhylomeDBiQ7Y232

Family and domain databases

InterProiView protein in InterPro
IPR002213 UDP_glucos_trans
IPR035595 UDP_glycos_trans_CS
PfamiView protein in Pfam
PF00201 UDPGT, 1 hit
PROSITEiView protein in PROSITE
PS00375 UDPGT, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7Y232-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNREQIHILF FPFMAHGHMI PLLDMAKLFA RRGAKSTLLT TPINAKILEK
60 70 80 90 100
PIEAFKVQNP DLEIGIKILN FPCVELGLPE GCENRDFINS YQKSDSFDLF
110 120 130 140 150
LKFLFSTKYM KQQLESFIET TKPSALVADM FFPWATESAE KIGVPRLVFH
160 170 180 190 200
GTSSFALCCS YNMRIHKPHK KVASSSTPFV IPGLPGDIVI TEDQANVTNE
210 220 230 240 250
ETPFGKFWKE VRESETSSFG VLVNSFYELE SSYADFYRSF VAKKAWHIGP
260 270 280 290 300
LSLSNRGIAE KAGRGKKANI DEQECLKWLD SKTPGSVVYL SFGSGTGLPN
310 320 330 340 350
EQLLEIAFGL EGSGQNFIWV VSKNENQVGT GENEDWLPKG FEERNKGKGL
360 370 380 390 400
IIRGWAPQVL ILDHKAIGGF VTHCGWNSTL EGIAAGLPMV TWPMGAEQFY
410 420 430 440 450
NEKLLTKVLR IGVNVGATEL VKKGKLISRA QVEKAVREVI GGEKAEERRL
460 470 480
RAKELGEMAK AAVEEGGSSY NDVNKFMEEL NGRK
Length:484
Mass (Da):53,961
Last modified:October 1, 2003 - v1
Checksum:i41C07469E63747D7
GO
Isoform 2 (identifier: Q7Y232-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     189-402: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:270
Mass (Da):30,278
Checksum:i5CEF0CE3B3D1A3BE
GO

Sequence cautioni

The sequence AAD17393 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_041227189 – 402Missing in isoform 2. CuratedAdd BLAST214

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006248 Genomic DNA Translation: AAD17393.1 Different initiation.
CP002685 Genomic DNA Translation: AEC06407.1
CP002685 Genomic DNA Translation: AEC06408.1
BT008319 mRNA Translation: AAP37678.1
AK227684 mRNA Translation: BAE99671.1
PIRiF84529
RefSeqiNP_179151.2, NM_127109.3 [Q7Y232-1]
NP_973469.1, NM_201740.1 [Q7Y232-2]
UniGeneiAt.40404

Genome annotation databases

EnsemblPlantsiAT2G15490.1; AT2G15490.1; AT2G15490 [Q7Y232-1]
AT2G15490.2; AT2G15490.2; AT2G15490 [Q7Y232-2]
GeneIDi816041
GrameneiAT2G15490.1; AT2G15490.1; AT2G15490 [Q7Y232-1]
AT2G15490.2; AT2G15490.2; AT2G15490 [Q7Y232-2]
KEGGiath:AT2G15490

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiU73B4_ARATH
AccessioniPrimary (citable) accession number: Q7Y232
Secondary accession number(s): Q3EBZ8, Q9ZQG3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: October 1, 2003
Last modified: April 25, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health