Q7Y223 (MAN2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mannan endo-1,4-beta-mannosidase 2
EC=3.2.1.78

Alternative name(s):
Beta-mannanase 2
Endo-beta-1,4-mannanase 2
Short name=AtMAN2

Gene names

Name:	MAN2
Ordered Locus Names:	At2g20680
ORF Names:	F5H14.35

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Subcellular location	Secreted Potential.
Tissue specificity	Expressed in roots, stems, leaves and seeds. Ref.6
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.
Sequence caution	The sequence AAD20927.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	mannan endo-1,4-beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 433	405	Mannan endo-1,4-beta-mannosidase 2		PRO_0000277475
Sites
	Active site	215	1	Proton donor By similarity
	Active site	335	1	Nucleophile By similarity
Amino acid modifications
	Glycosylation	46	1	N-linked (GlcNAc...) Potential
	Glycosylation	169	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q7Y223 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: DBC5FEBE6749B2D3	FASTA	433	49,558
	10 20 30 40 50 60 MAAPTGNGPV IPILGFLTCV AFIYLSFGDL WFGLKTEGEL AFVKRNGTQF VVDGKALYVN 70 80 90 100 110 120 GWNSYWFMDH AVNDHSRHRV SAMLEAGAKM GLTVCRTWAF NDGGYNALQI SPGRFDERVF 130 140 150 160 170 180 KALDHVIAEA KTHGVRLLLS LVNNLQAYGG KTQYVNWAWQ EGVGLSSSND SFFFDPSIRR 190 200 210 220 230 240 YFKNYLTVLL TRKNSLTGIE YRNDPTIFAW ELINEPRCMS DVSGDTLQDW INEMTAFIKS 250 260 270 280 290 300 IDNKHLLTVG LEGFYGPSSP KKLTVNPERW ASELGSDFVR NSDSPNIDFA SVHIYPDHWF 310 320 330 340 350 360 HDQGFEEKLK FVVKWMLSHI EDGDKELKKP VLFTEFGLSN LNKDYDPSQR DRFYRTIFDV 370 380 390 400 410 420 IYKSAKRKRS GAGTLVWQFL IEGMEGFNDD FGIVPWEQDS IQRLMIEQSC RLSRITGRHL 430 LDKKSIEMCS HRP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Shinn P., Chen H., Kim C.J., Quinitio C., Ecker J.R. Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar." Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F. Funct. Integr. Genomics 7:1-16(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC006234 Genomic DNA. Translation: AAD20927.1. Sequence problems. CP002685 Genomic DNA. Translation: AEC07056.1. BT008596 mRNA. Translation: AAP40422.1. BT025988 mRNA. Translation: ABG25077.1. AK228569 mRNA. Translation: BAF00487.1.
IPI	IPI00533463.
PIR	A84592.
RefSeq	NP_179660.2. NM_127632.2.
UniGene	At.39660.
3D structure databases
ProteinModelPortal	Q7Y223.
SMR	Q7Y223. Positions 41-395.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT2G20680.1-P.
Protein family/group databases
CAZy	GH5. Glycoside Hydrolase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G20680.1; AT2G20680.1; AT2G20680.
GeneID	816596.
KEGG	ath:AT2G20680.
Organism-specific databases
TAIR	At2g20680.
Phylogenomic databases
eggNOG	COG3934.
HOGENOM	HOG000169952.
InParanoid	Q7Y223.
OMA	INEPRCM.
PhylomeDB	Q7Y223.
ProtClustDB	CLSN2685722.
Gene expression databases
Genevestigator	Q7Y223.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001547. Glyco_hydro_5. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00150. Cellulase. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAN2_ARATH

Accession

Primary (citable) accession number: Q7Y223
Secondary accession number(s): Q9SKU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	October 1, 2003
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program