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Q7Y223

- MAN2_ARATH

UniProt

Q7Y223 - MAN2_ARATH

Protein

Mannan endo-1,4-beta-mannosidase 2

Gene

MAN2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei215 – 2151Proton donorBy similarity
    Active sitei335 – 3351NucleophileBy similarity

    GO - Molecular functioni

    1. mannan endo-1,4-beta-mannosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT2G20680-MONOMER.

    Protein family/group databases

    CAZyiGH5. Glycoside Hydrolase Family 5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mannan endo-1,4-beta-mannosidase 2 (EC:3.2.1.78)
    Alternative name(s):
    Beta-mannanase 2
    Endo-beta-1,4-mannanase 2
    Short name:
    AtMAN2
    Gene namesi
    Name:MAN2
    Ordered Locus Names:At2g20680
    ORF Names:F5H14.35
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G20680.

    Subcellular locationi

    Secreted Curated

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2828Sequence AnalysisAdd
    BLAST
    Chaini29 – 433405Mannan endo-1,4-beta-mannosidase 2PRO_0000277475Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi46 – 461N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi169 – 1691N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ7Y223.

    Expressioni

    Tissue specificityi

    Expressed in roots, stems, leaves and seeds.1 Publication

    Gene expression databases

    GenevestigatoriQ7Y223.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G20680.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7Y223.
    SMRiQ7Y223. Positions 41-395.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3934.
    HOGENOMiHOG000169952.
    InParanoidiQ7Y223.
    OMAiRRYFKNY.
    PhylomeDBiQ7Y223.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001547. Glyco_hydro_5.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00150. Cellulase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q7Y223-1 [UniParc]FASTAAdd to Basket

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    MAAPTGNGPV IPILGFLTCV AFIYLSFGDL WFGLKTEGEL AFVKRNGTQF    50
    VVDGKALYVN GWNSYWFMDH AVNDHSRHRV SAMLEAGAKM GLTVCRTWAF 100
    NDGGYNALQI SPGRFDERVF KALDHVIAEA KTHGVRLLLS LVNNLQAYGG 150
    KTQYVNWAWQ EGVGLSSSND SFFFDPSIRR YFKNYLTVLL TRKNSLTGIE 200
    YRNDPTIFAW ELINEPRCMS DVSGDTLQDW INEMTAFIKS IDNKHLLTVG 250
    LEGFYGPSSP KKLTVNPERW ASELGSDFVR NSDSPNIDFA SVHIYPDHWF 300
    HDQGFEEKLK FVVKWMLSHI EDGDKELKKP VLFTEFGLSN LNKDYDPSQR 350
    DRFYRTIFDV IYKSAKRKRS GAGTLVWQFL IEGMEGFNDD FGIVPWEQDS 400
    IQRLMIEQSC RLSRITGRHL LDKKSIEMCS HRP 433
    Length:433
    Mass (Da):49,558
    Last modified:October 1, 2003 - v1
    Checksum:iDBC5FEBE6749B2D3
    GO

    Sequence cautioni

    The sequence AAD20927.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006234 Genomic DNA. Translation: AAD20927.1. Sequence problems.
    CP002685 Genomic DNA. Translation: AEC07056.1.
    BT008596 mRNA. Translation: AAP40422.1.
    BT025988 mRNA. Translation: ABG25077.1.
    AK228569 mRNA. Translation: BAF00487.1.
    PIRiA84592.
    RefSeqiNP_179660.2. NM_127632.2.
    UniGeneiAt.39660.

    Genome annotation databases

    EnsemblPlantsiAT2G20680.1; AT2G20680.1; AT2G20680.
    GeneIDi816596.
    KEGGiath:AT2G20680.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006234 Genomic DNA. Translation: AAD20927.1 . Sequence problems.
    CP002685 Genomic DNA. Translation: AEC07056.1 .
    BT008596 mRNA. Translation: AAP40422.1 .
    BT025988 mRNA. Translation: ABG25077.1 .
    AK228569 mRNA. Translation: BAF00487.1 .
    PIRi A84592.
    RefSeqi NP_179660.2. NM_127632.2.
    UniGenei At.39660.

    3D structure databases

    ProteinModelPortali Q7Y223.
    SMRi Q7Y223. Positions 41-395.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT2G20680.1-P.

    Protein family/group databases

    CAZyi GH5. Glycoside Hydrolase Family 5.

    Proteomic databases

    PRIDEi Q7Y223.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G20680.1 ; AT2G20680.1 ; AT2G20680 .
    GeneIDi 816596.
    KEGGi ath:AT2G20680.

    Organism-specific databases

    TAIRi AT2G20680.

    Phylogenomic databases

    eggNOGi COG3934.
    HOGENOMi HOG000169952.
    InParanoidi Q7Y223.
    OMAi RRYFKNY.
    PhylomeDBi Q7Y223.

    Enzyme and pathway databases

    BioCyci ARA:AT2G20680-MONOMER.

    Gene expression databases

    Genevestigatori Q7Y223.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001547. Glyco_hydro_5.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00150. Cellulase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Arabidopsis ORF clones."
      Shinn P., Chen H., Kim C.J., Quinitio C., Ecker J.R.
      Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar."
      Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F.
      Funct. Integr. Genomics 7:1-16(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiMAN2_ARATH
    AccessioniPrimary (citable) accession number: Q7Y223
    Secondary accession number(s): Q9SKU9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 6, 2007
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3