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Q7Y223

- MAN2_ARATH

UniProt

Q7Y223 - MAN2_ARATH

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Protein

Mannan endo-1,4-beta-mannosidase 2

Gene

MAN2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei215 – 2151Proton donorBy similarity
Active sitei335 – 3351NucleophileBy similarity

GO - Molecular functioni

  1. mannan endo-1,4-beta-mannosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT2G20680-MONOMER.

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan endo-1,4-beta-mannosidase 2 (EC:3.2.1.78)
Alternative name(s):
Beta-mannanase 2
Endo-beta-1,4-mannanase 2
Short name:
AtMAN2
Gene namesi
Name:MAN2
Ordered Locus Names:At2g20680
ORF Names:F5H14.35
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G20680.

Subcellular locationi

Secreted Curated

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence AnalysisAdd
BLAST
Chaini29 – 433405Mannan endo-1,4-beta-mannosidase 2PRO_0000277475Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi46 – 461N-linked (GlcNAc...)Sequence Analysis
Glycosylationi169 – 1691N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ7Y223.

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves and seeds.1 Publication

Gene expression databases

GenevestigatoriQ7Y223.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G20680.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ7Y223.
SMRiQ7Y223. Positions 41-412.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3934.
HOGENOMiHOG000169952.
InParanoidiQ7Y223.
OMAiRRYFKNY.
PhylomeDBiQ7Y223.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7Y223-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAPTGNGPV IPILGFLTCV AFIYLSFGDL WFGLKTEGEL AFVKRNGTQF
60 70 80 90 100
VVDGKALYVN GWNSYWFMDH AVNDHSRHRV SAMLEAGAKM GLTVCRTWAF
110 120 130 140 150
NDGGYNALQI SPGRFDERVF KALDHVIAEA KTHGVRLLLS LVNNLQAYGG
160 170 180 190 200
KTQYVNWAWQ EGVGLSSSND SFFFDPSIRR YFKNYLTVLL TRKNSLTGIE
210 220 230 240 250
YRNDPTIFAW ELINEPRCMS DVSGDTLQDW INEMTAFIKS IDNKHLLTVG
260 270 280 290 300
LEGFYGPSSP KKLTVNPERW ASELGSDFVR NSDSPNIDFA SVHIYPDHWF
310 320 330 340 350
HDQGFEEKLK FVVKWMLSHI EDGDKELKKP VLFTEFGLSN LNKDYDPSQR
360 370 380 390 400
DRFYRTIFDV IYKSAKRKRS GAGTLVWQFL IEGMEGFNDD FGIVPWEQDS
410 420 430
IQRLMIEQSC RLSRITGRHL LDKKSIEMCS HRP
Length:433
Mass (Da):49,558
Last modified:October 1, 2003 - v1
Checksum:iDBC5FEBE6749B2D3
GO

Sequence cautioni

The sequence AAD20927.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006234 Genomic DNA. Translation: AAD20927.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC07056.1.
BT008596 mRNA. Translation: AAP40422.1.
BT025988 mRNA. Translation: ABG25077.1.
AK228569 mRNA. Translation: BAF00487.1.
PIRiA84592.
RefSeqiNP_179660.2. NM_127632.2.
UniGeneiAt.39660.

Genome annotation databases

EnsemblPlantsiAT2G20680.1; AT2G20680.1; AT2G20680.
GeneIDi816596.
KEGGiath:AT2G20680.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006234 Genomic DNA. Translation: AAD20927.1 . Sequence problems.
CP002685 Genomic DNA. Translation: AEC07056.1 .
BT008596 mRNA. Translation: AAP40422.1 .
BT025988 mRNA. Translation: ABG25077.1 .
AK228569 mRNA. Translation: BAF00487.1 .
PIRi A84592.
RefSeqi NP_179660.2. NM_127632.2.
UniGenei At.39660.

3D structure databases

ProteinModelPortali Q7Y223.
SMRi Q7Y223. Positions 41-412.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT2G20680.1-P.

Protein family/group databases

CAZyi GH5. Glycoside Hydrolase Family 5.

Proteomic databases

PRIDEi Q7Y223.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G20680.1 ; AT2G20680.1 ; AT2G20680 .
GeneIDi 816596.
KEGGi ath:AT2G20680.

Organism-specific databases

TAIRi AT2G20680.

Phylogenomic databases

eggNOGi COG3934.
HOGENOMi HOG000169952.
InParanoidi Q7Y223.
OMAi RRYFKNY.
PhylomeDBi Q7Y223.

Enzyme and pathway databases

BioCyci ARA:AT2G20680-MONOMER.

Gene expression databases

Genevestigatori Q7Y223.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00150. Cellulase. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clones."
    Shinn P., Chen H., Kim C.J., Quinitio C., Ecker J.R.
    Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar."
    Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F.
    Funct. Integr. Genomics 7:1-16(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, TISSUE SPECIFICITY.

Entry informationi

Entry nameiMAN2_ARATH
AccessioniPrimary (citable) accession number: Q7Y223
Secondary accession number(s): Q9SKU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: October 1, 2003
Last modified: November 26, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3