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Q7Y223 (MAN2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mannan endo-1,4-beta-mannosidase 2

EC=3.2.1.78
Alternative name(s):
Beta-mannanase 2
Endo-beta-1,4-mannanase 2
Short name=AtMAN2
Gene names
Name:MAN2
Ordered Locus Names:At2g20680
ORF Names:F5H14.35
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length433 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Subcellular location

Secreted Potential.

Tissue specificity

Expressed in roots, stems, leaves and seeds. Ref.6

Sequence similarities

Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Sequence caution

The sequence AAD20927.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmannan endo-1,4-beta-mannosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Potential
Chain29 – 433405Mannan endo-1,4-beta-mannosidase 2
PRO_0000277475

Sites

Active site2151Proton donor By similarity
Active site3351Nucleophile By similarity

Amino acid modifications

Glycosylation461N-linked (GlcNAc...) Potential
Glycosylation1691N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q7Y223 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: DBC5FEBE6749B2D3

FASTA43349,558
        10         20         30         40         50         60 
MAAPTGNGPV IPILGFLTCV AFIYLSFGDL WFGLKTEGEL AFVKRNGTQF VVDGKALYVN 

        70         80         90        100        110        120 
GWNSYWFMDH AVNDHSRHRV SAMLEAGAKM GLTVCRTWAF NDGGYNALQI SPGRFDERVF 

       130        140        150        160        170        180 
KALDHVIAEA KTHGVRLLLS LVNNLQAYGG KTQYVNWAWQ EGVGLSSSND SFFFDPSIRR 

       190        200        210        220        230        240 
YFKNYLTVLL TRKNSLTGIE YRNDPTIFAW ELINEPRCMS DVSGDTLQDW INEMTAFIKS 

       250        260        270        280        290        300 
IDNKHLLTVG LEGFYGPSSP KKLTVNPERW ASELGSDFVR NSDSPNIDFA SVHIYPDHWF 

       310        320        330        340        350        360 
HDQGFEEKLK FVVKWMLSHI EDGDKELKKP VLFTEFGLSN LNKDYDPSQR DRFYRTIFDV 

       370        380        390        400        410        420 
IYKSAKRKRS GAGTLVWQFL IEGMEGFNDD FGIVPWEQDS IQRLMIEQSC RLSRITGRHL 

       430 
LDKKSIEMCS HRP 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Arabidopsis ORF clones."
Shinn P., Chen H., Kim C.J., Quinitio C., Ecker J.R.
Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"The endo-beta-mannanase gene families in Arabidopsis, rice, and poplar."
Yuan J.S., Yang X., Lai J., Lin H., Cheng Z.-M., Nonogaki H., Chen F.
Funct. Integr. Genomics 7:1-16(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC006234 Genomic DNA. Translation: AAD20927.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC07056.1.
BT008596 mRNA. Translation: AAP40422.1.
BT025988 mRNA. Translation: ABG25077.1.
AK228569 mRNA. Translation: BAF00487.1.
PIRA84592.
RefSeqNP_179660.2. NM_127632.2.
UniGeneAt.39660.

3D structure databases

ProteinModelPortalQ7Y223.
SMRQ7Y223. Positions 41-395.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT2G20680.1-P.

Protein family/group databases

CAZyGH5. Glycoside Hydrolase Family 5.

Proteomic databases

PRIDEQ7Y223.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G20680.1; AT2G20680.1; AT2G20680.
GeneID816596.
KEGGath:AT2G20680.

Organism-specific databases

TAIRAT2G20680.

Phylogenomic databases

eggNOGCOG3934.
HOGENOMHOG000169952.
InParanoidQ7Y223.
OMARRYFKNY.
PhylomeDBQ7Y223.

Enzyme and pathway databases

BioCycARA:AT2G20680-MONOMER.

Gene expression databases

GenevestigatorQ7Y223.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMAN2_ARATH
AccessionPrimary (citable) accession number: Q7Y223
Secondary accession number(s): Q9SKU9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: October 1, 2003
Last modified: June 11, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names