Q7Y201 (PME13_ARATH) Reviewed, UniProtKB/Swiss-Prot
Last modified
September 21, 2011.
Version 52.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Probable pectinesterase/pectinesterase inhibitor 13 Including the following 2 domains:
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| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) | ||||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Protein attributes
| Sequence length | 614 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Acts in the modification of cell walls via demethylesterification of cell wall pectin By similarity. |
| Catalytic activity | Pectin + n H2O = n methanol + pectate. |
| Pathway | Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5. |
| Subcellular location | Membrane; Single-pass membrane protein Potential. |
| Tissue specificity | Expressed in flower buds. Ref.6 |
| Miscellaneous | The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport. |
| Sequence similarities | In the N-terminal section; belongs to the PMEI family. In the C-terminal section; belongs to the pectinesterase family. |
| Sequence caution | The sequence AAC14494.1 differs from that shown. Reason: Erroneous initiation. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Membrane |
| Domain | Transmembrane Transmembrane helix |
| Molecular function | Aspartyl esterase Hydrolase |
| PTM | Disulfide bond Glycoprotein |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | cell wall modification Inferred from electronic annotation. Source: InterPro |
| Cellular component | cell wall Inferred from electronic annotation. Source: InterPro integral to membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | aspartyl esterase activity Inferred from electronic annotation. Source: UniProtKB-KW enzyme inhibitor activityInferred from electronic annotation. Source: InterPro pectinesterase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 614 | 614 | Probable pectinesterase/pectinesterase inhibitor 13 | PRO_0000371670 | |||||||
Regions | |||||||||||
| Transmembrane | 25 – 45 | 21 | Helical; Potential | ||||||||
| Region | 103 – 255 | 153 | Pectinesterase inhibitor 13 | ||||||||
| Region | 301 – 598 | 298 | Pectinesterase 13 | ||||||||
| Compositional bias | 55 – 61 | 7 | Poly-Gln | ||||||||
Sites | |||||||||||
| Active site | 429 | 1 | Proton donor; for pectinesterase activity By similarity | ||||||||
| Active site | 450 | 1 | Nucleophile; for pectinesterase activity By similarity | ||||||||
| Binding site | 376 | 1 | Substrate; for pectinesterase activity By similarity | ||||||||
| Binding site | 406 | 1 | Substrate; for pectinesterase activity By similarity | ||||||||
| Binding site | 518 | 1 | Substrate; for pectinesterase activity By similarity | ||||||||
| Binding site | 520 | 1 | Substrate; for pectinesterase activity By similarity | ||||||||
| Site | 428 | 1 | Transition state stabilizer By similarity | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 66 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 128 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 197 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 243 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 301 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 351 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 367 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 522 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 588 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 443 ↔ 463 | By similarity | |||||||||
Experimental info | |||||||||||
| Sequence conflict | 277 | 1 | D → G in AAP40488. Ref.3 | ||||||||
| Sequence conflict | 277 | 1 | D → G in BAF01447. Ref.4 | ||||||||
| Sequence conflict | 402 | 1 | P → S in AAP40488. Ref.3 | ||||||||
| Sequence conflict | 402 | 1 | P → S in BAF01447. Ref.4 | ||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C.Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [2] | The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia. |
| [3] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [4] | "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. Shinozaki K.Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [5] | "Pectin methylesterases: sequence-structural features and phylogenetic relationships." Markovic O., Janecek S. Carbohydr. Res. 339:2281-2295(2004) [PubMed: 15337457] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE. |
| [6] | "Comprehensive expression profiling of the pectin methylesterase gene family during silique development in Arabidopsis thaliana." Louvet R., Cavel E., Gutierrez L., Guenin S., Roger D., Gillet F., Guerineau F., Pelloux J. Planta 224:782-791(2006) [PubMed: 16622707] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AC002505 Genomic DNA. Translation: AAC14494.1. Different initiation. CP002685 Genomic DNA. Translation: AEC07840.1. BT008681 mRNA. Translation: AAP40488.1. AK229600 mRNA. Translation: BAF01447.1. |
| IPI | IPI00521390. |
| PIR | T00978. |
| RefSeq | NP_850077.1. NM_179746.1. |
| UniGene | At.38894. At.70844. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1GQ8 based on UniProtKB P83218. |
| ProteinModelPortal | Q7Y201. |
| SMR | Q7Y201. Positions 296-611. |
| ModBase | Search... |
Proteomic databases | |
| PRIDE | Q7Y201. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblPlants | AT2G26450.1; AT2G26450.1; AT2G26450. |
| GeneID | 817185. |
| GenomeReviews | Gene locus AT2G26450 in contig CT485783_GR. |
| KEGG | ath:AT2G26450. |
Organism-specific databases | |
| GeneFarm | 229. 8. |
| TAIR | At2g26450. |
Phylogenomic databases | |
| eggNOG | euNOG09608. |
| GeneTree | EPGT00070000028024. |
| InParanoid | Q7Y201. |
| OMA | QGDWISA. |
Gene expression databases | |
| Genevestigator | Q7Y201. |
Family and domain databases | |
| InterPro | IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. IPR018040. Pectinesterase_AS. IPR000070. Pectinesterase_cat. IPR006501. Pectinesterase_inhib. [Graphical view] |
| Gene3D | G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit. G3DSA:1.20.140.40. Pectinesterase_inhib. 1 hit. |
| Pfam | PF01095. Pectinesterase. 1 hit. PF04043. PMEI. 1 hit. [Graphical view] |
| SMART | SM00856. PMEI. 1 hit. [Graphical view] |
| SUPFAM | SSF51126. Pectin_lyas_like. 1 hit. SSF101148. Pectinesterase_inhib. 1 hit. |
| TIGRFAMs | TIGR01614. PME_inhib. 1 hit. |
| PROSITE | PS00800. PECTINESTERASE_1. False negative. PS00503. PECTINESTERASE_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | PME13_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q7Y201 Secondary accession number(s): O48712 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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