Q7Y1Z0 (CHI5_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitinase 5
EC=3.2.1.14

Alternative name(s):
Class IV chitinase a
Short name=OsChia4a
Pathogenesis related (PR)-3 chitinase 5

Gene names

Name:	Cht5
Ordered Locus Names:	Os04g0494100, LOC_Os04g41680
ORF Names:	OsJ_15308, OSJNBb0091E11.12

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May function in reproductive organs during embryogenesis and seed maturation.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Tissue specificity	Expressed in sheaths and meristems and at lower levels in roots and leaves. Ref.7
Sequence similarities	Belongs to the glycosyl hydrolase 19 family. Chitinase class IV subfamily. Contains 1 chitin-binding type-1 domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Plant defense Polysaccharide degradation
Domain	Signal
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW defense response Inferred from electronic annotation. Source: UniProtKB-KW polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW somatic embryogenesis Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	apoplast Inferred from electronic annotation. Source: EnsemblPlants/Gramene plant-type cell wall Inferred from electronic annotation. Source: EnsemblPlants/Gramene plasma membrane Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 288	259	Chitinase 5		PRO_0000383464
Regions
	Domain	30 – 64	35	Chitin-binding type-1
	Compositional bias	64 – 84	21	Poly-Gly
Amino acid modifications
	Disulfide bond	32 ↔ 40		By similarity
	Disulfide bond	34 ↔ 46		By similarity
	Disulfide bond	39 ↔ 53		By similarity
	Disulfide bond	57 ↔ 62		By similarity
	Disulfide bond	107 ↔ 156		By similarity
	Disulfide bond	169 ↔ 178		By similarity
	Disulfide bond	256 ↔ 288		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7Y1Z0 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: E988A12134910C52	FASTA	288	30,487
	10 20 30 40 50 60 MANSPTPTML AFLALGLALL LSATGQASAQ NCGCQSNMCC SKWGYCGTGK DYCGDGCRSG 70 80 90 100 110 120 PCYGGGGGGG GGGGGGGGGG GGSGVSVESV VTEAFFNGIK NQAPNGCAGK NFYTRQSFLN 130 140 150 160 170 180 AAHSYSGFAR DRTNDDSKRE IAAFFAHVTH ETGHMCYINE INGASMDYCD KNNKQWPCQP 190 200 210 220 230 240 GKKYYGRGPL QISWNYNYGP AGQNIGFDGL RDPDRVAQDP TISFKTALWF WMNNVHQVML 250 260 270 280 QGFGATIRAI NGALECNGKN PGAVNARVNY YKDYCRQFGV DPGGNLYC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure, heterologous expression, and properties of rice (Oryza sativa L.) family 19 chitinases." Truong N.-H., Park S.-M., Nishizawa Y., Watanabe T., Sasaki T., Itoh Y. Biosci. Biotechnol. Biochem. 67:1063-1070(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare.
[2]	"Sequence and analysis of rice chromosome 4." Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y., Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q., Zhang L. , Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T., Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R., Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G., Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C., Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J., Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J., Li J., Hong G., Xue Y., Han B. Nature 420:316-320(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[5]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[6]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[7]	"Distribution, structure, organ-specific expression, and phylogenic analysis of the pathogenesis-related protein-3 chitinase gene family in rice (Oryza sativa L.)." Nakazaki T., Tsukiyama T., Okumoto Y., Kageyama D., Naito K., Inouye K., Tanisaka T. Genome 49:619-630(2006) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB096140 mRNA. Translation: BAC76691.1. AL606629 Genomic DNA. Translation: CAE01675.2. AP008210 Genomic DNA. Translation: BAF15100.1. CM000141 Genomic DNA. Translation: EEE61251.1. AK071013 mRNA. Translation: BAG92259.1. AK104242 mRNA. Translation: BAG96537.1. AK104426 mRNA. Translation: BAG96672.1.
RefSeq	NP_001053186.1. NM_001059721.1.
UniGene	Os.10166.
3D structure databases
HSSP	HSSP built from PDB template 1MMC based on UniProtKB P27275.
ProteinModelPortal	Q7Y1Z0.
ModBase	Search...
Protein family/group databases
CAZy	CBM18. Carbohydrate-Binding Module Family 18. GH19. Glycoside Hydrolase Family 19.
Proteomic databases
PaxDb	Q7Y1Z0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os04g41680.1; LOC_Os04g41680.1; LOC_Os04g41680.
GeneID	4336265.
KEGG	osa:4336265.
Organism-specific databases
Gramene	Q7Y1Z0.
Phylogenomic databases
eggNOG	COG3979.
KO	K01183.
OMA	MENAPRI.
ProtClustDB	CLSN2725090.
Gene expression databases
ArrayExpress	Q7Y1Z0.
Family and domain databases
Gene3D	3.30.60.10. 1 hit.
InterPro	IPR001002. Chitin-bd_1. IPR018371. Chitin-binding_1_CS. IPR016283. Glyco_hydro_19. IPR000726. Glyco_hydro_19_cat. IPR023346. Lysozyme-like_dom. [Graphical view]
Pfam	PF00187. Chitin_bind_1. 1 hit. PF00182. Glyco_hydro_19. 1 hit. [Graphical view]
PIRSF	PIRSF001060. Endochitinase. 1 hit.
ProDom	PD000609. Chitin_bd_1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00270. ChtBD1. 1 hit. [Graphical view]
SUPFAM	SSF57016. Chitin_bd_1. 1 hit. SSF53955. SSF53955. 1 hit.
PROSITE	PS00026. CHIT_BIND_I_1. 1 hit. PS50941. CHIT_BIND_I_2. 1 hit. PS00773. CHITINASE_19_1. 1 hit. PS00774. CHITINASE_19_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHI5_ORYSJ

Accession

Primary (citable) accession number: Q7Y1Z0
Secondary accession number(s): Q7XT47

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	October 1, 2003
Last modified:	April 3, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program