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Protein

L-ascorbate peroxidase 5, peroxisomal

Gene

APX5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a key role in hydrogen peroxide removal.By similarity

Miscellaneous

Binds one cation per subunit; probably K+, but might also be Ca2+.By similarity

Catalytic activityi

2 L-ascorbate + H2O2 + 2 H+ = L-ascorbate + L-dehydroascorbate + 2 H2O.

Cofactori

heme bNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei35Transition state stabilizerPROSITE-ProRule annotation1
Active sitei39Proton acceptorPROSITE-ProRule annotation1
Metal bindingi158Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi159Potassium or calciumBy similarity1
Metal bindingi175Potassium or calciumBy similarity1
Metal bindingi182Potassium or calciumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase, Peroxidase
Biological processHydrogen peroxide
LigandCalcium, Heme, Iron, Metal-binding, Potassium

Enzyme and pathway databases

BioCyciARA:AT4G35970-MONOMER

Protein family/group databases

PeroxiBasei1887 AtAPx04

Names & Taxonomyi

Protein namesi
Recommended name:
L-ascorbate peroxidase 5, peroxisomal (EC:1.11.1.11)
Short name:
AtAPx04
Gene namesi
Name:APX5
Ordered Locus Names:At4g35970
ORF Names:F4B14_240, T19K4.100
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G35970
TAIRilocus:2125409 AT4G35970

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei251 – 271HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000261324? – 279L-ascorbate peroxidase 5, peroxisomal
Transit peptidei1 – ?PeroxisomeSequence analysis

Proteomic databases

PaxDbiQ7XZP5

Expressioni

Gene expression databases

ExpressionAtlasiQ7XZP5 baseline and differential
GenevisibleiQ7XZP5 AT

Interactioni

Subunit structurei

Interacts with AKR2A and AKR2B.1 Publication

Protein-protein interaction databases

BioGridi15033, 2 interactors
STRINGi3702.AT4G35970.1

Structurei

3D structure databases

ProteinModelPortaliQ7XZP5
SMRiQ7XZP5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi272 – 279AKR2A-binding sequence (ABS) required for peroxisome membrane targeting1 Publication8

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEWS Eukaryota
COG0376 LUCA
HOGENOMiHOG000189824
InParanoidiQ7XZP5
KOiK00434
OMAiTICYEAS
OrthoDBiEOG09360FM3
PhylomeDBiQ7XZP5

Family and domain databases

InterProiView protein in InterPro
IPR010255 Haem_peroxidase
IPR002016 Haem_peroxidase_pln/fun/bac
IPR002207 Peroxidase_I
IPR019794 Peroxidases_AS
IPR019793 Peroxidases_heam-ligand_BS
PfamiView protein in Pfam
PF00141 peroxidase, 1 hit
PRINTSiPR00459 ASPEROXIDASE
PR00458 PEROXIDASE
SUPFAMiSSF48113 SSF48113, 1 hit
PROSITEiView protein in PROSITE
PS00435 PEROXIDASE_1, 1 hit
PS00436 PEROXIDASE_2, 1 hit
PS50873 PEROXIDASE_4, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7XZP5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVNVDAEYL KEIEKTRRDL RALISSRNCA PIMLRLAWHD AGTYDAKKKT
60 70 80 90 100
GGANGSIRFK EELNRPHNKG LEKAVAFCEE VKAKHPRVSY ADLYQLAGVV
110 120 130 140 150
AVEVTGGPAI PFTPGRKDAD SADDGELPNP NEGASHLRTL FSRMGLLDRD
160 170 180 190 200
IVALSGGHTL GRAHKERSDF EGPWTQDPLK FDNSYFVELL KGETPGLLQL
210 220 230 240 250
KTDKALLDDP KFHPFVKLYA KDEDMFFKAY AISHKKLSEL GFNPPRRIPS
260 270
AVTQQTLGIA VAAAVVIFTI CYEASRRGK
Length:279
Mass (Da):30,895
Last modified:November 28, 2006 - v2
Checksum:i518836C729B1C745
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL022373 Genomic DNA Translation: CAA18491.1
AL031986 Genomic DNA Translation: CAA21483.1
AL161588 Genomic DNA Translation: CAB81506.1
CP002687 Genomic DNA Translation: AEE86596.1
AK119023 mRNA Translation: BAC43599.1
BT006053 mRNA Translation: AAP04038.1
AF441714 mRNA Translation: AAP72144.1
PIRiT04707
RefSeqiNP_195321.1, NM_119763.3
UniGeneiAt.31367

Genome annotation databases

EnsemblPlantsiAT4G35970.1; AT4G35970.1; AT4G35970
GeneIDi829751
GrameneiAT4G35970.1; AT4G35970.1; AT4G35970
KEGGiath:AT4G35970

Similar proteinsi

Entry informationi

Entry nameiAPX5_ARATH
AccessioniPrimary (citable) accession number: Q7XZP5
Secondary accession number(s): O65634
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 28, 2006
Last modified: April 25, 2018
This is version 97 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health