Reviewed, UniProtKB/Swiss-Prot Q7XZF7 (GYRA_ORYSJ)

Names and origin

Protein names

Recommended name:
    Probable DNA gyrase subunit A, chloroplastic/mitochondrial
    EC=5.99.1.3

Gene names

Name:	GYRA
Ordered Locus Names:	Os03g0812000, LOC_Os03g59750
ORF Names:	OSJNBa0024F18.37, OSJNBb0033J23.17

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	939 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis By similarity.
Subcellular location	Plastid › chloroplast Potential. Mitochondrion Potential.
Sequence similarities	Belongs to the topoisomerase gyrA/parC subunit family.
Sequence caution	The sequence AAP73860.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAR89868.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Chloroplast Mitochondrion Plastid
Domain	Transit peptide
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell chromosome Inferred from electronic annotation. Source: InterPro mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast and mitochondrion Potential
	Chain	? – 939		Probable DNA gyrase subunit A, chloroplastic/mitochondrial		PRO_0000247947
Regions
	Compositional bias	46 – 52	7	Poly-Ser
Sites
	Active site	196	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7XZF7-1 [UniParc]. Last modified July 25, 2006. Version 2. Checksum: 88439FE6D72B88E9	FASTA	939	103,168
	10 20 30 40 50 60 MALSAALRLP LPRLLWGPTG SLLAAAAAAS RRRAAVVAVP AVRFLSSSSS SSDGSRSVQP 70 80 90 100 110 120 LRAGRDERAA AGEGGAAVKE RVVPVELHKE ATEAYMAYAM SVLLGRALPD VRDGLKPVHR 130 140 150 160 170 180 RILYAMHEMG LASRRPFRKC ARVVGEVLGK FHPHGDSAVY ETLVRMAQDF SMRYPLVQGH 190 200 210 220 230 240 GNFGSIDADP PAAMRYTECR LDVGCFFFLD IDAIFIIGQV DFVPNFDNSQ KEPSLLPARV 250 260 270 280 290 300 PSLLLNGSSG IAVGMATNIP PHNLGELVDV LSVMIENPEA TLQELLECMP GPDFPTGGTI 310 320 330 340 350 360 VGNQGILEAY KTGRGRVVMR GKTDIETIDV KSKRSAIIIK EVPYQTNKST LVERIAELVE 370 380 390 400 410 420 EKVLEGISDI RDESDRSGMR VVIELKRGAD PAIVLNNLYR HTALQSSFSC NMVAILDGQP 430 440 450 460 470 480 KLMGLKEILQ AFIDFRCSVI ERRARFKLSQ ALERKHIVEG IVIGLDNLDS VIQIIRGTSN 490 500 510 520 530 540 HAMARESLIK EFGLSDKQAE ALLDITLRKL TSLERKKFVD EAKSLSEEIS KLNELLSNKK 550 560 570 580 590 600 LIFQLILQEA TDLKNKFATP RRSFIEDSAS TEVDDLDIIP NEEMLLILSE KGYVKRMKPN 610 620 630 640 650 660 TFNLQNRGTI GKSVGKMRMN DNTSDFIVCQ THDHVLYFSD KGIVYSARAY KIPECTRAAT 670 680 690 700 710 720 GTPLVQLLSL SDGERITSIV PVNEFGEDQY LVMLTVNGYI KKVPLNAFSA IRTSGIISIQ 730 740 750 760 770 780 LAPGDELKWV RRCGDDDLVA LASQNGMVIV NTCNKLRALG RKTRGVLAMK LKEGDKMASM 790 800 810 820 830 840 DIIPATSHNM PETYSRVRDL SPPWLLFIAD NGIGKRVPLN AFRQGNFNRV GLQGYKLPPD 850 860 870 880 890 900 CSLAAVFVVG FSLTDDGESD EQVVLVSQSG TVNRIKVKDI SIRSRSARGV ILMRLEHAGK 910 920 930 IQSASLISAA EEEEEQDPES ASLISEAEEP EKQDPEVSA

References

[1]

"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S. Genome Res. 15:1284-1291(2005) [PubMed: 16109971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

[2]

"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 502-939. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC135594 Genomic DNA. Translation: AAR89868.1. Sequence problems. AC137507 Genomic DNA. Translation: AAP73860.1. Sequence problems. AK059281 mRNA. No translation available.
3D structure databases
HSSP	HSSP built from PDB template 1AB4 based on UniProtKB P09097.
ModBase	Search...
Organism-specific databases
Gramene	Q7XZF7.
Family and domain databases
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
ProDom	PD000742. DNA_topoisoIV. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
TIGRFAMs	TIGR01063. gyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_ORYSJ

Accession

Primary (citable) accession number: Q7XZF7
Secondary accession number(s): Q75HI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 25, 2006
Last modified:	July 7, 2009
This is version 38 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)