Q7XUK4 (GUN12_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase 12
EC=3.2.1.4

Alternative name(s):
Endo-1,4-beta glucanase 12
OsGLU3

Gene names

Name:	GLU3
Ordered Locus Names:	Os04g0497200, LOC_Os04g41970
ORF Names:	OSJNBa0067K08.14

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	623 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Membrane; Single-pass type II membrane protein Potential.
Tissue specificity	Ubiquitous. Ref.5
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.
Sequence caution	The sequence BAF15118.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 623	623	Endoglucanase 12		PRO_0000249289
Regions
	Topological domain	1 – 73	73	Cytoplasmic Potential
	Transmembrane	74 – 94	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	95 – 623	529	Extracellular Potential
Sites
	Active site	513	1	By similarity
	Active site	561	1	By similarity
	Active site	570	1	By similarity
Amino acid modifications
	Glycosylation	217	1	N-linked (GlcNAc...) Potential
	Glycosylation	236	1	N-linked (GlcNAc...) Potential
	Glycosylation	324	1	N-linked (GlcNAc...) Potential
	Glycosylation	345	1	N-linked (GlcNAc...) Potential
	Glycosylation	408	1	N-linked (GlcNAc...) Potential
	Glycosylation	425	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q7XUK4 [UniParc]. Last modified March 1, 2004. Version 2. Checksum: BAEF21481AEF18B2	FASTA	623	69,243
	10 20 30 40 50 60 MYSANHWGGS FEIAADGAAE DDHSRNMDLD RGALSARQHQ LDETQQSWLL GPPEAKKKDK 70 80 90 100 110 120 YVDLGCVVVK RKLLWWVLWT LLAAFILIGL PVIIAKSIPK KKPHAPPPDQ YTDALHKALL 130 140 150 160 170 180 FFNAQKSGRL PKNNGIKWRG NSGLSDGSDL TDVKGGLVGG YYDAGDNIKF HFPLAFSMTM 190 200 210 220 230 240 LSWSVIEYSA KYKAVGEYDH VRELIKWGTD YLLLTFNSSA STIDKVYSQV GIAKINGTQP 250 260 270 280 290 300 DDHYCWNRPE DMAYPRPVQT AGSAPDLGGE MAAALAAASI VFRDNAAYSK KLVNGAAAVY 310 320 330 340 350 360 KFARSSGRRT PYSRGNQYIE YYYNSTSYWD EYMWSAAWMY YATGNNTYIT FATDPRLPKN 370 380 390 400 410 420 AKAFYSILDF SVFSWDNKLP GAELLLSRLR MFLNPGYPYE ESLIGYHNTT SMNMCTYFPR 430 440 450 460 470 480 FGAFNFTKGG LAQFNHGKGQ PLQYTVANSF LAALYADYME SVNVPGWYCG PYFMTVDDLR 490 500 510 520 530 540 SFARSQVNYI LGDNPKKMSY VVGYGKKYPR RLHHRGASTP HNGIKYSCTG GYKWRDTKGA 550 560 570 580 590 600 DPNVLVGAMV GGPDKNDQFK DARLTYAQNE PTLVGNAGLV AALVALTNSG RGAGVTAVDK 610 620 NTMFSAVPPM FPATPPPPSK WKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of rice chromosome 4." Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y., Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q., Zhang L. , Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T., Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R., Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G., Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C., Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J., Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J., Li J., Hong G., Xue Y., Han B. Nature 420:316-320(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Oryza sativa full length cDNA." The rice full-length cDNA consortium Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[5]	"OsGLU1, a putative membrane-bound endo-1,4-beta-D-glucanase from rice, affects plant internode elongation." Zhou H.-L., He S.-J., Cao Y.-R., Chen T., Du B.-X., Chu C.-C., Zhang J.-S., Chen S.-Y. Plant Mol. Biol. 60:137-151(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL606627 Genomic DNA. Translation: CAD41250.2. AP008210 Genomic DNA. Translation: BAF15118.2. Sequence problems. AK243593 mRNA. No translation available.
RefSeq	NP_001053204.2. NM_001059739.2.
UniGene	Os.49714. Os.58516.
3D structure databases
HSSP	HSSP built from PDB template 1TF4 based on UniProtKB P26221.
ProteinModelPortal	Q7XUK4.
ModBase	Search...
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Proteomic databases
PRIDE	Q7XUK4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os04g41970.1; LOC_Os04g41970.1; LOC_Os04g41970.
GeneID	4336284.
KEGG	osa:4336284.
Organism-specific databases
Gramene	Q7XUK4.
Phylogenomic databases
eggNOG	NOG307789.
HOGENOM	HOG000021033.
OMA	QCASAST.
ProtClustDB	CLSN2679649.
Gene expression databases
ArrayExpress	Q7XUK4.
Family and domain databases
Gene3D	1.50.10.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN12_ORYSJ

Accession

Primary (citable) accession number: Q7XUK4
Secondary accession number(s): Q0JC19

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	March 1, 2004
Last modified:	April 3, 2013
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program