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Protein

Endoglucanase 12

Gene

GLU3

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei513 – 5131By similarity
Active sitei561 – 5611By similarity
Active sitei570 – 5701By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cell wall biogenesis/degradation, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase 12 (EC:3.2.1.4)
Alternative name(s):
Endo-1,4-beta glucanase 12
OsGLU3
Gene namesi
Name:GLU3
Ordered Locus Names:Os04g0497200, LOC_Os04g41970
ORF Names:OSJNBa0067K08.14
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
ProteomesiUP000000763 Componenti: Chromosome 4

Organism-specific databases

GrameneiQ7XUK4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7373CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei74 – 9421Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini95 – 623529ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 623623Endoglucanase 12PRO_0000249289Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi217 – 2171N-linked (GlcNAc...)Sequence Analysis
Glycosylationi236 – 2361N-linked (GlcNAc...)Sequence Analysis
Glycosylationi324 – 3241N-linked (GlcNAc...)Sequence Analysis
Glycosylationi345 – 3451N-linked (GlcNAc...)Sequence Analysis
Glycosylationi408 – 4081N-linked (GlcNAc...)Sequence Analysis
Glycosylationi425 – 4251N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ7XUK4.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

ExpressionAtlasiQ7XUK4. baseline.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os04g41970.1.

Structurei

3D structure databases

ProteinModelPortaliQ7XUK4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG307789.
HOGENOMiHOG000021033.
InParanoidiQ7XUK4.
OMAiQCASAST.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamiPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7XUK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYSANHWGGS FEIAADGAAE DDHSRNMDLD RGALSARQHQ LDETQQSWLL
60 70 80 90 100
GPPEAKKKDK YVDLGCVVVK RKLLWWVLWT LLAAFILIGL PVIIAKSIPK
110 120 130 140 150
KKPHAPPPDQ YTDALHKALL FFNAQKSGRL PKNNGIKWRG NSGLSDGSDL
160 170 180 190 200
TDVKGGLVGG YYDAGDNIKF HFPLAFSMTM LSWSVIEYSA KYKAVGEYDH
210 220 230 240 250
VRELIKWGTD YLLLTFNSSA STIDKVYSQV GIAKINGTQP DDHYCWNRPE
260 270 280 290 300
DMAYPRPVQT AGSAPDLGGE MAAALAAASI VFRDNAAYSK KLVNGAAAVY
310 320 330 340 350
KFARSSGRRT PYSRGNQYIE YYYNSTSYWD EYMWSAAWMY YATGNNTYIT
360 370 380 390 400
FATDPRLPKN AKAFYSILDF SVFSWDNKLP GAELLLSRLR MFLNPGYPYE
410 420 430 440 450
ESLIGYHNTT SMNMCTYFPR FGAFNFTKGG LAQFNHGKGQ PLQYTVANSF
460 470 480 490 500
LAALYADYME SVNVPGWYCG PYFMTVDDLR SFARSQVNYI LGDNPKKMSY
510 520 530 540 550
VVGYGKKYPR RLHHRGASTP HNGIKYSCTG GYKWRDTKGA DPNVLVGAMV
560 570 580 590 600
GGPDKNDQFK DARLTYAQNE PTLVGNAGLV AALVALTNSG RGAGVTAVDK
610 620
NTMFSAVPPM FPATPPPPSK WKP
Length:623
Mass (Da):69,243
Last modified:March 1, 2004 - v2
Checksum:iBAEF21481AEF18B2
GO

Sequence cautioni

The sequence BAF15118.2 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL606627 Genomic DNA. Translation: CAD41250.2.
AP008210 Genomic DNA. Translation: BAF15118.2. Sequence problems.
AK243593 mRNA. No translation available.
RefSeqiNP_001053204.2. NM_001059739.2.
UniGeneiOs.49714.
Os.58516.

Genome annotation databases

EnsemblPlantsiOS04T0497200-01; OS04T0497200-01; OS04G0497200.
GeneIDi4336284.
KEGGiosa:4336284.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL606627 Genomic DNA. Translation: CAD41250.2.
AP008210 Genomic DNA. Translation: BAF15118.2. Sequence problems.
AK243593 mRNA. No translation available.
RefSeqiNP_001053204.2. NM_001059739.2.
UniGeneiOs.49714.
Os.58516.

3D structure databases

ProteinModelPortaliQ7XUK4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os04g41970.1.

Protein family/group databases

CAZyiGH9. Glycoside Hydrolase Family 9.

Proteomic databases

PRIDEiQ7XUK4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS04T0497200-01; OS04T0497200-01; OS04G0497200.
GeneIDi4336284.
KEGGiosa:4336284.

Organism-specific databases

GrameneiQ7XUK4.

Phylogenomic databases

eggNOGiNOG307789.
HOGENOMiHOG000021033.
InParanoidiQ7XUK4.
OMAiQCASAST.

Gene expression databases

ExpressionAtlasiQ7XUK4. baseline.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamiPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. "Oryza sativa full length cDNA."
    The rice full-length cDNA consortium
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.
  5. "OsGLU1, a putative membrane-bound endo-1,4-beta-D-glucanase from rice, affects plant internode elongation."
    Zhou H.-L., He S.-J., Cao Y.-R., Chen T., Du B.-X., Chu C.-C., Zhang J.-S., Chen S.-Y.
    Plant Mol. Biol. 60:137-151(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiGUN12_ORYSJ
AccessioniPrimary (citable) accession number: Q7XUK4
Secondary accession number(s): Q0JC19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: March 1, 2004
Last modified: June 24, 2015
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.