Reviewed, UniProtKB/Swiss-Prot Q7XUK4 (GUN12_ORYSJ)

Names and origin

Protein names

Recommended name:
    Endoglucanase 12
    EC=3.2.1.4
Alternative name(s):
    Endo-1,4-beta glucanase 12
    OsGLU3

Gene names

Name:	GLU3
Ordered Locus Names:	Os04g0497200, LOC_Os04g41970
ORF Names:	OSJNBa0067K08.14

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	623 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Membrane; Single-pass type II membrane protein Potential.
Tissue specificity	Ubiquitous. Ref.5
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 623	623	Endoglucanase 12		PRO_0000249289
Regions
	Topological domain	1 – 73	73	Cytoplasmic Potential
	Transmembrane	74 – 94	21	Signal-anchor for type II membrane protein Potential
	Topological domain	95 – 623	529	Extracellular Potential
Sites
	Active site	513	1	By similarity
	Active site	561	1	By similarity
	Active site	570	1	By similarity
Amino acid modifications
	Glycosylation	217	1	N-linked (GlcNAc...) Potential
	Glycosylation	236	1	N-linked (GlcNAc...) Potential
	Glycosylation	324	1	N-linked (GlcNAc...) Potential
	Glycosylation	345	1	N-linked (GlcNAc...) Potential
	Glycosylation	408	1	N-linked (GlcNAc...) Potential
	Glycosylation	425	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q7XUK4-1 [UniParc]. Last modified March 1, 2004. Version 2. Checksum: BAEF21481AEF18B2	FASTA	623	69,243
	10 20 30 40 50 60 MYSANHWGGS FEIAADGAAE DDHSRNMDLD RGALSARQHQ LDETQQSWLL GPPEAKKKDK 70 80 90 100 110 120 YVDLGCVVVK RKLLWWVLWT LLAAFILIGL PVIIAKSIPK KKPHAPPPDQ YTDALHKALL 130 140 150 160 170 180 FFNAQKSGRL PKNNGIKWRG NSGLSDGSDL TDVKGGLVGG YYDAGDNIKF HFPLAFSMTM 190 200 210 220 230 240 LSWSVIEYSA KYKAVGEYDH VRELIKWGTD YLLLTFNSSA STIDKVYSQV GIAKINGTQP 250 260 270 280 290 300 DDHYCWNRPE DMAYPRPVQT AGSAPDLGGE MAAALAAASI VFRDNAAYSK KLVNGAAAVY 310 320 330 340 350 360 KFARSSGRRT PYSRGNQYIE YYYNSTSYWD EYMWSAAWMY YATGNNTYIT FATDPRLPKN 370 380 390 400 410 420 AKAFYSILDF SVFSWDNKLP GAELLLSRLR MFLNPGYPYE ESLIGYHNTT SMNMCTYFPR 430 440 450 460 470 480 FGAFNFTKGG LAQFNHGKGQ PLQYTVANSF LAALYADYME SVNVPGWYCG PYFMTVDDLR 490 500 510 520 530 540 SFARSQVNYI LGDNPKKMSY VVGYGKKYPR RLHHRGASTP HNGIKYSCTG GYKWRDTKGA 550 560 570 580 590 600 DPNVLVGAMV GGPDKNDQFK DARLTYAQNE PTLVGNAGLV AALVALTNSG RGAGVTAVDK 610 620 NTMFSAVPPM FPATPPPPSK WKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of rice chromosome 4." Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y., Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q., Zhang L. , Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T., Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R., Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G., Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C., Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J., Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J., Li J., Hong G., Xue Y., Han B. Nature 420:316-320(2002) [PubMed: 12447439] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Oryza sativa full length cDNA." The rice full-length cDNA consortium Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[5]	"OsGLU1, a putative membrane-bound endo-1,4-beta-D-glucanase from rice, affects plant internode elongation." Zhou H.-L., He S.-J., Cao Y.-R., Chen T., Du B.-X., Chu C.-C., Zhang J.-S., Chen S.-Y. Plant Mol. Biol. 60:137-151(2006) [PubMed: 16463105] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL606627 Genomic DNA. Translation: CAD41250.2. AP008210 Genomic DNA. Translation: BAF15118.1. AK243593 mRNA. No translation available.
RefSeq	NP_001053204.1.
UniGene	Os.49714 Os.58516
3D structure databases
HSSP	HSSP built from PDB template 1IA6 based on UniProtKB Q9EYQ2.
ModBase	Search...
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Genome annotation databases
GeneID	4336284.
GenomeReviews	Gene locus GLU3 in contig AP008210_GR.
KEGG	osa:4336284.
Organism-specific databases
Gramene	Q7XUK4.
Family and domain databases
InterPro	IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Gene3D	G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
PANTHER	PTHR22298:SF3. Glyco_hydro_9. 1 hit.
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN12_ORYSJ

Accession

Primary (citable) accession number: Q7XUK4
Secondary accession number(s): Q0JC19

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	March 1, 2004
Last modified:	October 13, 2009
This is version 37 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)