Q7XRB5 (PDI12_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein disulfide isomerase-like 1-2
Short name=OsPDIL1-2
EC=5.3.4.1

Gene names

Name:	PDIL1-2
Ordered Locus Names:	Os04g0436300, LOC_Os04g35600
ORF Names:	OSJNBa0006B20.4

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	517 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts as a protein-folding catalyst that interacts with nascent polypeptides to catalyze the formation, isomerization, and reduction or oxidation of disulfide bonds. May play a role in storage protein biogenesis By similarity.
Catalytic activity	Catalyzes the rearrangement of -S-S- bonds in proteins.
Subcellular location	Endoplasmic reticulum lumen Probable.
Sequence similarities	Belongs to the protein disulfide isomerase family. Contains 2 thioredoxin domains.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Redox-active center Repeat Signal
Molecular function	Isomerase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro embryo development Inferred from electronic annotation. Source: EnsemblPlants/Gramene glycerol ether metabolic process Inferred from electronic annotation. Source: InterPro protein folding Inferred from electronic annotation. Source: GOC regulation of programmed cell death Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to cadmium ion Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to endoplasmic reticulum stress Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to salt stress Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to zinc ion Inferred from electronic annotation. Source: EnsemblPlants/Gramene seed development Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	chloroplast Inferred from electronic annotation. Source: EnsemblPlants/Gramene endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell lytic vacuole within protein storage vacuole Inferred from electronic annotation. Source: EnsemblPlants/Gramene plant-type cell wall Inferred from electronic annotation. Source: EnsemblPlants/Gramene plasma membrane Inferred from electronic annotation. Source: EnsemblPlants/Gramene thylakoid Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro protein disulfide isomerase activity Inferred from electronic annotation. Source: EC protein disulfide oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 517	494	Protein disulfide isomerase-like 1-2		PRO_0000400029
Regions
	Domain	24 – 143	120	Thioredoxin 1
	Domain	357 – 484	128	Thioredoxin 2
	Motif	514 – 517	4	Prevents secretion from ER By similarity
Sites
	Active site	61	1	Nucleophile By similarity
	Active site	64	1	Nucleophile By similarity
	Active site	407	1	Nucleophile By similarity
	Active site	410	1	Nucleophile By similarity
	Site	62	1	Contributes to redox potential value By similarity
	Site	63	1	Contributes to redox potential value By similarity
	Site	129	1	Lowers pKa of C-terminal Cys of first active site By similarity
	Site	408	1	Contributes to redox potential value By similarity
	Site	409	1	Contributes to redox potential value By similarity
	Site	470	1	Lowers pKa of C-terminal Cys of second active site By similarity
Amino acid modifications
	Glycosylation	41	1	N-linked (GlcNAc...) Potential
	Glycosylation	301	1	N-linked (GlcNAc...) Potential
	Disulfide bond	61 ↔ 64		Redox-active By similarity
	Disulfide bond	407 ↔ 410		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7XRB5 [UniParc]. Last modified March 1, 2004. Version 2. Checksum: B7DB4CEEEE6AAD65	FASTA	517	57,335
	10 20 30 40 50 60 MAVNLVLSFA LAILISSSPT AVGVDATEEL KEAVLTLDAG NFSEVVAKHP FIVVKFYAPW 70 80 90 100 110 120 CGHCKQLAPE YEKAASILRK NELPVVLAKV DAYNERNKEL KDKYGVYSYP TIKIMKNGGS 130 140 150 160 170 180 DVRGYGGPRE ADGIVEYLKR QVGPASLKLE SAEEAAHSVV DKGVILVGVF PEFAGMEYEN 190 200 210 220 230 240 FMVVAEKMRA DYDFFHTSDA SILPRGDQSV KGPIVRLFKP FDELFVDSED FGKDALEKFI 250 260 270 280 290 300 EVSGFPMVVT YDADPTNHKF LERYYSTPSS KAMLFVSFGD DRIESFKSQI HEAARKFSGN 310 320 330 340 350 360 NISFLIGDVA DADRVFQYFG LRESDVPLLF VIASTGKYLN PTMDPDQIIP WLKQYIVEYG 370 380 390 400 410 420 NLTPYVKSEP IPKVNDQPVK VVVADNIDDI VFNSGKNVLL EFYAPWCGHC RKFALILEEI 430 440 450 460 470 480 AVSLQDDQDI VIAKMDGTVN DIPTDFTVEG YPTIYFYSSS GNLLSYDGAR TAEEIISFIN 490 500 510 ENRGPKAGAA AAVDEKTQID AVEEEVTSSS EPVKDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Phylogenetic analyses identify 10 classes of the protein disulfide isomerase family in plants, including single-domain protein disulfide isomerase-related proteins." Houston N.L., Fan C., Xiang J.Q., Schulze J.M., Jung R., Boston R.S. Plant Physiol. 137:762-778(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE. Strain: cv. Nipponbare.
[2]	"Sequence and analysis of rice chromosome 4." Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y., Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q., Zhang L. , Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T., Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R., Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G., Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C., Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J., Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J., Li J., Hong G., Xue Y., Han B. Nature 420:316-320(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[5]	"The protein disulfide isomerase gene family in bread wheat (T. aestivum L.)." d'Aloisio E., Paolacci A.R., Dhanapal A.P., Tanzarella O.A., Porceddu E., Ciaffi M. BMC Plant Biol. 10:101-101(2010) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY739308 mRNA. Translation: AAX14679.1. AL606592 Genomic DNA. Translation: CAE02742.2. AP008210 Genomic DNA. Translation: BAF14766.2.
RefSeq	NP_001052852.2. NM_001059387.2.
UniGene	Os.53966.
3D structure databases
HSSP	HSSP built from PDB template 1FAA based on UniProtKB P09856.
ProteinModelPortal	Q7XRB5.
ModBase	Search...
Proteomic databases
PRIDE	Q7XRB5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os04g35600.1; LOC_Os04g35600.1; LOC_Os04g35600.
GeneID	4335906.
KEGG	osa:4335906.
Organism-specific databases
Gramene	Q7XRB5.
Phylogenomic databases
eggNOG	COG0526.
HOGENOM	HOG000162459.
KO	K09580.
OMA	LERYYST.
Gene expression databases
ArrayExpress	Q7XRB5.
Family and domain databases
Gene3D	3.40.30.10. 3 hits.
InterPro	IPR005788. Disulphide_isomerase. IPR005792. Prot_disulphide_isomerase. IPR005746. Thioredoxin. IPR012336. Thioredoxin-like_fold. IPR017937. Thioredoxin_CS. IPR013766. Thioredoxin_domain. [Graphical view]
Pfam	PF00085. Thioredoxin. 2 hits. [Graphical view]
PRINTS	PR00421. THIOREDOXIN.
SUPFAM	SSF52833. Thiordxn-like_fd. 4 hits.
TIGRFAMs	TIGR01130. ER_PDI_fam. 1 hit. TIGR01126. pdi_dom. 1 hit.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00194. THIOREDOXIN_1. 2 hits. PS51352. THIOREDOXIN_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDI12_ORYSJ

Accession

Primary (citable) accession number: Q7XRB5
Secondary accession number(s): Q0JD21

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 2, 2010
Last sequence update:	March 1, 2004
Last modified:	April 3, 2013
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program