Q7XR06 (P2C45_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable protein phosphatase 2C 45
Short name=OsPP2C45
EC=3.1.3.16

Gene names

Ordered Locus Names:	Os04g0659500, LOC_Os04g56450
ORF Names:	OsJ_015762, OSJNBa0011F23.20, OSJNBa0015K02.7

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response to bacterium Inferred from electronic annotation. Source: EnsemblPlants/Gramene protein dephosphorylation Inferred from electronic annotation. Source: InterPro
Cellular_component	plasma membrane Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q7XR06-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q7XR06-2)

The sequence of this isoform differs from the canonical sequence as follows:
     25-64: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	Probable protein phosphatase 2C 45		PRO_0000363292
Regions
	Domain	26 – 265	240	PP2C-like
Sites
	Metal binding	63	1	Manganese 1 By similarity
	Metal binding	63	1	Manganese 2 By similarity
	Metal binding	64	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	224	1	Manganese 2 By similarity
	Metal binding	263	1	Manganese 2 By similarity
Natural variations
	Alternative sequence	25 – 64	40	Missing in isoform 2.		VSP_036269

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2004. Version 2. Checksum: B8FF3CF119534AB7	FASTA	282	30,584
	10 20 30 40 50 60 MGYLSSVIPT DGSPVSGGGL SQNGKFSYGY ASSPGKRASM EDFYETRIDS VDGQIIGLFG 70 80 90 100 110 120 VFDGHGGAKV AEYVKQNLFS HLLRHPKFIS DTKVAIDDAY KSTDSEFLES DSSQNQCGST 130 140 150 160 170 180 ASTAVLVGDR LFVANVGDSR AIICRGGNAI AVSKDHKPDQ TDERQRIEDA GGFVMWAGTW 190 200 210 220 230 240 RVGGVLAVSR AFGDKLLKQY VVVDPEIREE VIDHSLEFLI LASDGLWDVV TNEEAVDMTR 250 260 270 280 SIHDPEEAAK KLLQEAYKRE SSDNITCVVV RFLHGQGSSG YA
	Isoform 2 [UniParc]. Checksum: 8CC4D5FE1B05EA83	FASTA	242	26,190
	10 20 30 40 50 60 MGYLSSVIPT DGSPVSGGGL SQNGHGGAKV AEYVKQNLFS HLLRHPKFIS DTKVAIDDAY 70 80 90 100 110 120 KSTDSEFLES DSSQNQCGST ASTAVLVGDR LFVANVGDSR AIICRGGNAI AVSKDHKPDQ 130 140 150 160 170 180 TDERQRIEDA GGFVMWAGTW RVGGVLAVSR AFGDKLLKQY VVVDPEIREE VIDHSLEFLI 190 200 210 220 230 240 LASDGLWDVV TNEEAVDMTR SIHDPEEAAK KLLQEAYKRE SSDNITCVVV RFLHGQGSSG YA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of rice chromosome 4." Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y., Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q., Zhang L. , Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T., Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R., Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G., Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C., Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J., Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J., Li J., Hong G., Xue Y., Han B. Nature 420:316-320(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[5]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: cv. Nipponbare.
[6]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL606608 Genomic DNA. Translation: CAE02890.2. AL662953 Genomic DNA. Translation: CAE54579.1. AP008210 Genomic DNA. Translation: BAF16051.1. CM000141 Genomic DNA. Translation: EAZ32279.1. AK059302 mRNA. Translation: BAG86954.1. AK072534 mRNA. Translation: BAG93020.1.
RefSeq	NP_001054137.1. NM_001060672.1.
UniGene	Os.172.
3D structure databases
HSSP	HSSP built from PDB template 1A6Q based on UniProtKB P35813.
ProteinModelPortal	Q7XR06.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os04g56450.1; LOC_Os04g56450.1; LOC_Os04g56450.
GeneID	4337276.
KEGG	osa:4337276.
Organism-specific databases
Gramene	Q7XR06.
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000233896.
OMA	ACITEAY.
Gene expression databases
ArrayExpress	Q7XR06.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR000222. PP2C_Mn2_Asp60_BS. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C45_ORYSJ

Accession

Primary (citable) accession number: Q7XR06
Secondary accession number(s): Q0J9D6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	March 1, 2004
Last modified:	April 3, 2013
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program