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Q7XKV4

- BGL12_ORYSJ

UniProt

Q7XKV4 - BGL12_ORYSJ

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Protein

Beta-glucosidase 12

Gene

BGLU12

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Hydrolyzes p-nitrophenyl beta-D-glucoside, p-nitrophenyl beta-D-galactoside, p-nitrophenyl beta-D-xyloside, p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-arabinoside, cello-oligosaccharides and laminaribiose.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei53 – 531SubstrateBy similarity
Binding sitei157 – 1571SubstrateBy similarity
Binding sitei202 – 2021SubstrateBy similarity
Active sitei203 – 2031Proton donorBy similarity
Binding sitei346 – 3461SubstrateBy similarity
Active sitei417 – 4171NucleophileBy similarity
Binding sitei466 – 4661SubstrateBy similarity

GO - Molecular functioni

  1. beta-D-fucosidase activity Source: UniProtKB
  2. beta-galactosidase activity Source: UniProtKB
  3. beta-gentiobiose beta-glucosidase activity Source: UniProtKB
  4. beta-glucosidase activity Source: UniProtKB
  5. beta-L-arabinosidase activity Source: UniProtKB
  6. glucan exo-1,3-beta-glucosidase activity Source: UniProtKB

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucosidase 12 (EC:3.2.1.21)
Short name:
Os4bglu12
Gene namesi
Name:BGLU12
Ordered Locus Names:Os04g0474800, LOC_Os04g39880
ORF Names:OsJ_15166, OSJNBa0022H21.3
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 4

Organism-specific databases

GrameneiQ7XKV4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 510486Beta-glucosidase 12PRO_0000390329Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi122 – 1221N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi222 ↔ 230By similarity
Glycosylationi229 – 2291N-linked (GlcNAc...)Sequence Analysis
Glycosylationi361 – 3611N-linked (GlcNAc...)Sequence Analysis
Glycosylationi371 – 3711N-linked (GlcNAc...)Sequence Analysis
Glycosylationi425 – 4251N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os09g36810.1.

Structurei

3D structure databases

ProteinModelPortaliQ7XKV4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni473 – 4742Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088630.
InParanoidiQ7XKV4.
KOiK01188.
OMAiNDFKDYA.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q7XKV4-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAGAMPGG LLLTFLLLAV VASGAYNGAG EPPVSRRSFP KGFIFGTASS
60 70 80 90 100
SYQYEGGAAE GGRGPSIWDT FTHQHPEKIA DRSNGDVASD SYHLYKEDVR
110 120 130 140 150
LMKDMGMDAY RFSISWTRIL PNGSLRGGVN KEGIKYYNNL INELLSKGVQ
160 170 180 190 200
PFITLFHWDS PQALEDKYNG FLSPNIINDF KDYAEICFKE FGDRVKNWIT
210 220 230 240 250
FNEPWTFCSN GYATGLFAPG RCSPWEKGNC SVGDSGREPY TACHHQLLAH
260 270 280 290 300
AETVRLYKAK YQALQKGKIG ITLVSHWFVP FSRSKSNDDA AKRAIDFMFG
310 320 330 340 350
WFMDPLIRGD YPLSMRGLVG NRLPQFTKEQ SKLVKGAFDF IGLNYYTANY
360 370 380 390 400
ADNLPPSNGL NNSYTTDSRA NLTGVRNGIP IGPQAASPWL YVYPQGFRDL
410 420 430 440 450
LLYVKENYGN PTVYITENGV DEFNNKTLPL QEALKDDARI EYYHKHLLSL
460 470 480 490 500
LSAIRDGANV KGYFAWSLLD NFEWSNGYTV RFGINFVDYN DGRKRYPKNS
510
AHWFKKFLLK

Note: Derived from EST data. No experimental confirmation available.

Length:510
Mass (Da):57,464
Last modified:March 1, 2004 - v2
Checksum:i5828169EA16037DA
GO
Isoform 2 (identifier: Q7XKV4-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-119: Missing.
     120-122: LPN → MAD

Note: No experimental confirmation available.

Show »
Length:391
Mass (Da):44,662
Checksum:i4979F83BD1BF4B2E
GO

Sequence cautioni

The sequence BAF14984.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 119119Missing in isoform 2. 1 PublicationVSP_038503Add
BLAST
Alternative sequencei120 – 1223LPN → MAD in isoform 2. 1 PublicationVSP_038504

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL731582 Genomic DNA. Translation: CAE05483.2.
AP008210 Genomic DNA. Translation: BAF14984.1. Sequence problems.
CM000141 Genomic DNA. Translation: EEE61179.1.
AK100820 mRNA. Translation: BAG94781.1.
RefSeqiNP_001053070.1. NM_001059605.1.
UniGeneiOs.59390.

Genome annotation databases

GeneIDi4336145.
KEGGiosa:4336145.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL731582 Genomic DNA. Translation: CAE05483.2 .
AP008210 Genomic DNA. Translation: BAF14984.1 . Sequence problems.
CM000141 Genomic DNA. Translation: EEE61179.1 .
AK100820 mRNA. Translation: BAG94781.1 .
RefSeqi NP_001053070.1. NM_001059605.1.
UniGenei Os.59390.

3D structure databases

ProteinModelPortali Q7XKV4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 39947.LOC_Os09g36810.1.

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 4336145.
KEGGi osa:4336145.

Organism-specific databases

Gramenei Q7XKV4.

Phylogenomic databases

eggNOGi COG2723.
HOGENOMi HOG000088630.
InParanoidi Q7XKV4.
KOi K01188.
OMAi NDFKDYA.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 1 hit.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. "The genomes of Oryza sativa: a history of duplications."
    Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
    , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
    PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  5. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Nipponbare.
  6. "Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 beta-glucosidase."
    Opassiri R., Pomthong B., Onkoksoong T., Akiyama T., Esen A., Ketudat Cairns J.R.
    BMC Plant Biol. 6:33-33(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiBGL12_ORYSJ
AccessioniPrimary (citable) accession number: Q7XKV4
Secondary accession number(s): B7EQY4, Q0JCF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: March 1, 2004
Last modified: October 29, 2014
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3