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Protein

Beta-glucosidase 12

Gene

BGLU12

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes p-nitrophenyl beta-D-glucoside, p-nitrophenyl beta-D-galactoside, p-nitrophenyl beta-D-xyloside, p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-arabinoside, cello-oligosaccharides and laminaribiose.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.1 Publication

Kineticsi

kcat is 18.5 (sec-1) with p-nitrophenyl beta-D-glucopyranoside as substrate. kcat is 0.27 (sec-1) with p-nitrophenyl beta-D-thioglucoside as substrate. kcat is 12.1 (sec-1) with n-heptyl-beta-D-glucoside as substrate. kcat is 9.8 (src-1) with n-octyl-beta-D-glucoside as substrate. kcat is 0.038 (sec-1) n-octyl-beta-D-thioglucopyranoside as substrate.1 Publication
  1. KM=0.56 mM for p-nitrophenyl beta-D-glucopyranoside1 Publication
  2. KM=1.64 mM for p-nitrophenyl beta-D-thioglucoside1 Publication
  3. KM=0.91 mM for n-heptyl-beta-D-glucoside1 Publication
  4. KM=0.44 mM for n-octyl-beta-D-glucoside1 Publication
  5. KM=1.61 mM for n-octyl-beta-D-thioglucopyranoside1 Publication
  1. Vmax=20.1 µmol/min/mg enzyme with p-nitrophenyl beta-D-glucopyranoside as substrate1 Publication
  2. Vmax=0.29 µmol/min/mg enzyme with p-nitrophenyl beta-D-thioglucoside as substrate1 Publication
  3. Vmax=13.2 µmol/min/mg enzyme with n-heptyl-beta-D-glucoside as substrate1 Publication
  4. Vmax=10.7 µmol/min/mg enzyme with n-octyl-beta-D-glucoside as substrate1 Publication
  5. Vmax=4 µmol/min/mg enzyme with n-octyl-beta-D-thioglucopyranoside as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei53SubstrateCombined sources1 Publication1
Binding sitei157SubstrateCombined sources1 Publication1
Binding sitei202SubstrateCombined sources1 Publication1
Active sitei203Proton donor1 Publication1
Binding sitei346SubstrateCombined sources1 Publication1
Active sitei417Nucleophile1 Publication1
Binding sitei466SubstrateBy similarity1

GO - Molecular functioni

  • beta-D-fucosidase activity Source: UniProtKB
  • beta-galactosidase activity Source: UniProtKB
  • beta-gentiobiose beta-glucosidase activity Source: UniProtKB
  • beta-glucosidase activity Source: UniProtKB
  • beta-L-arabinosidase activity Source: UniProtKB
  • glucan exo-1,3-beta-glucosidase activity Source: UniProtKB
  • scopolin beta-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

ReactomeiR-OSA-189085 Digestion of dietary carbohydrate

Protein family/group databases

CAZyiGH1 Glycoside Hydrolase Family 1

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucosidase 12Curated (EC:3.2.1.211 Publication)
Short name:
Os4bglu121 Publication
Gene namesi
Name:BGLU12Curated
Ordered Locus Names:Os04g0474800Imported, LOC_Os04g39880Curated
ORF Names:OsJ_15166Imported, OSJNBa0022H21.3Imported
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi276H → M: Decreases the kcat/Km values 2 to 6-fold depending on the substrate. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000039032925 – 510Beta-glucosidase 12Sequence analysisAdd BLAST486

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi122N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi208 ↔ 243Combined sources1 Publication
Disulfide bondi222 ↔ 230Combined sources1 Publication
Glycosylationi229N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi361N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi371N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi425N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ7XKV4

Expressioni

Gene expression databases

ExpressionAtlasiQ7XKV4 differential

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os04g39880.1

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PTKX-ray2.49A/B25-510[»]
3PTMX-ray2.40A/B25-510[»]
3PTQX-ray2.45A/B25-510[»]
ProteinModelPortaliQ7XKV4
SMRiQ7XKV4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni473 – 474Substrate bindingCombined sources1 Publication2

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0626 Eukaryota
COG2723 LUCA
HOGENOMiHOG000088630
InParanoidiQ7XKV4
KOiK01188
OMAiNRWWLDP

Family and domain databases

InterProiView protein in InterPro
IPR001360 Glyco_hydro_1
IPR033132 Glyco_hydro_1_N_CS
IPR017853 Glycoside_hydrolase_SF
PANTHERiPTHR10353 PTHR10353, 1 hit
PfamiView protein in Pfam
PF00232 Glyco_hydro_1, 1 hit
PRINTSiPR00131 GLHYDRLASE1
SUPFAMiSSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS00653 GLYCOSYL_HYDROL_F1_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7XKV4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAGAMPGG LLLTFLLLAV VASGAYNGAG EPPVSRRSFP KGFIFGTASS
60 70 80 90 100
SYQYEGGAAE GGRGPSIWDT FTHQHPEKIA DRSNGDVASD SYHLYKEDVR
110 120 130 140 150
LMKDMGMDAY RFSISWTRIL PNGSLRGGVN KEGIKYYNNL INELLSKGVQ
160 170 180 190 200
PFITLFHWDS PQALEDKYNG FLSPNIINDF KDYAEICFKE FGDRVKNWIT
210 220 230 240 250
FNEPWTFCSN GYATGLFAPG RCSPWEKGNC SVGDSGREPY TACHHQLLAH
260 270 280 290 300
AETVRLYKAK YQALQKGKIG ITLVSHWFVP FSRSKSNDDA AKRAIDFMFG
310 320 330 340 350
WFMDPLIRGD YPLSMRGLVG NRLPQFTKEQ SKLVKGAFDF IGLNYYTANY
360 370 380 390 400
ADNLPPSNGL NNSYTTDSRA NLTGVRNGIP IGPQAASPWL YVYPQGFRDL
410 420 430 440 450
LLYVKENYGN PTVYITENGV DEFNNKTLPL QEALKDDARI EYYHKHLLSL
460 470 480 490 500
LSAIRDGANV KGYFAWSLLD NFEWSNGYTV RFGINFVDYN DGRKRYPKNS
510
AHWFKKFLLK
Note: Derived from EST data. No experimental confirmation available.
Length:510
Mass (Da):57,464
Last modified:March 1, 2004 - v2
Checksum:i5828169EA16037DA
GO
Isoform 2 (identifier: Q7XKV4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-119: Missing.
     120-122: LPN → MAD

Note: No experimental confirmation available.
Show »
Length:391
Mass (Da):44,662
Checksum:i4979F83BD1BF4B2E
GO

Sequence cautioni

The sequence BAF14984 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0385031 – 119Missing in isoform 2. 1 PublicationAdd BLAST119
Alternative sequenceiVSP_038504120 – 122LPN → MAD in isoform 2. 1 Publication3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL731582 Genomic DNA Translation: CAE05483.2
AP008210 Genomic DNA Translation: BAF14984.1 Sequence problems.
AP014960 Genomic DNA Translation: BAS89674.1
CM000141 Genomic DNA Translation: EEE61179.1
AK100820 mRNA Translation: BAG94781.1
RefSeqiXP_015636597.1, XM_015781111.1 [Q7XKV4-1]
UniGeneiOs.59390

Genome annotation databases

EnsemblPlantsiOs04t0474800-02; Os04t0474800-02; Os04g0474800 [Q7XKV4-2]
GeneIDi4336145
GrameneiOs04t0474800-02; Os04t0474800-02; Os04g0474800 [Q7XKV4-2]
KEGGiosa:4336145

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiBGL12_ORYSJ
AccessioniPrimary (citable) accession number: Q7XKV4
Secondary accession number(s): B7EQY4, Q0JCF3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: March 1, 2004
Last modified: May 23, 2018
This is version 91 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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