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Protein

Calcium-dependent protein kinase 16

Gene

CPK16

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei137ATPPROSITE-ProRule annotation1
Active sitei234Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi114 – 122ATPPROSITE-ProRule annotation9
Calcium bindingi424 – 4351PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi461 – 4722PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi503 – 5143PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi533 – 5444PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase 16 (EC:2.7.11.1)
Gene namesi
Name:CPK16
Ordered Locus Names:At2g17890
ORF Names:T13L16.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G17890.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • nucleus Source: GO_Central
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2G → A: Loss of myristoylation. Loss of plasma membrane localization and relocation to nucleolus and chloroplasts. 1 Publication1
Mutagenesisi4C → A: Drastic reduction of plasma membrane localization and strong increase of nuclear localization. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCurated
ChainiPRO_00003633382 – 571Calcium-dependent protein kinase 16Add BLAST570

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1
Lipidationi4S-palmitoyl cysteine1 Publication1

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ7XJR9.
PRIDEiQ7XJR9.

PTM databases

iPTMnetiQ7XJR9.

Expressioni

Gene expression databases

GenevisibleiQ7XJR9. AT.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi1656. 1 interactor.
STRINGi3702.AT2G17890.1.

Structurei

3D structure databases

ProteinModelPortaliQ7XJR9.
SMRiQ7XJR9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini108 – 368Protein kinasePROSITE-ProRule annotationAdd BLAST261
Domaini411 – 447EF-hand 1PROSITE-ProRule annotationAdd BLAST37
Domaini448 – 483EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini484 – 525EF-hand 3PROSITE-ProRule annotationAdd BLAST42
Domaini526 – 555EF-hand 4PROSITE-ProRule annotationAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni374 – 404Autoinhibitory domainBy similarityAdd BLAST31

Domaini

There is 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (374-404) inactivates kinase activity under calcium-free conditions (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation
Contains 4 EF-hand domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
InParanoidiQ7XJR9.
KOiK13412.
OMAiKMTIPIA.
OrthoDBiEOG093605DQ.
PhylomeDBiQ7XJR9.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7XJR9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLCFSSAAK SSGHNRSSRN PHPHPPLTVV KSRPPRSPCS FMAVTIQKDH
60 70 80 90 100
RTQPRRNATA KKTPTRHTPP HGKVREKVIS NNGRRHGETI PYGKRVDFGY
110 120 130 140 150
AKDFDHRYTI GKLLGHGQFG YTYVATDKKT GDRVAVKKID KAKMTIPIAV
160 170 180 190 200
EDVKREVKIL QALTGHENVV RFYNAFEDKN SVYIVMELCE GGELLDRILA
210 220 230 240 250
RKDSRYSERD AAVVVRQMLK VAAECHLRGL VHRDMKPENF LFKSTEEDSP
260 270 280 290 300
LKATDFGLSD FIKPGKKFHD IVGSAYYVAP EVLKRRSGPE SDVWSIGVIS
310 320 330 340 350
YILLCGRRPF WDKTEDGIFK EVLKNKPDFR RKPWPTISNS AKDFVKKLLV
360 370 380 390 400
KDPRARLTAA QALSHPWVRE GGDASEIPID ISVLNNMRQF VKFSRLKQFA
410 420 430 440 450
LRALATTLDE EELADLRDQF DAIDVDKNGV ISLEEMRQAL AKDHPWKLKD
460 470 480 490 500
ARVAEILQAI DSNTDGFVDF GEFVAAALHV NQLEEHDSEK WQQRSRAAFE
510 520 530 540 550
KFDIDGDGFI TAEELRMHTG LKGSIEPLLE EADIDNDGKI SLQEFRRLLR
560 570
TASIKSRNVR SPPGYLISRK V
Length:571
Mass (Da):64,754
Last modified:October 1, 2003 - v1
Checksum:i282D5E81EA571D74
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002685 Genomic DNA. Translation: AEC06699.1.
PIRiT00835.
RefSeqiNP_179379.1. NM_127343.2.

Genome annotation databases

EnsemblPlantsiAT2G17890.1; AT2G17890.1; AT2G17890.
GeneIDi816299.
GrameneiAT2G17890.1; AT2G17890.1; AT2G17890.
KEGGiath:AT2G17890.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002685 Genomic DNA. Translation: AEC06699.1.
PIRiT00835.
RefSeqiNP_179379.1. NM_127343.2.

3D structure databases

ProteinModelPortaliQ7XJR9.
SMRiQ7XJR9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi1656. 1 interactor.
STRINGi3702.AT2G17890.1.

PTM databases

iPTMnetiQ7XJR9.

Proteomic databases

PaxDbiQ7XJR9.
PRIDEiQ7XJR9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G17890.1; AT2G17890.1; AT2G17890.
GeneIDi816299.
GrameneiAT2G17890.1; AT2G17890.1; AT2G17890.
KEGGiath:AT2G17890.

Organism-specific databases

TAIRiAT2G17890.

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
InParanoidiQ7XJR9.
KOiK13412.
OMAiKMTIPIA.
OrthoDBiEOG093605DQ.
PhylomeDBiQ7XJR9.

Miscellaneous databases

PROiQ7XJR9.

Gene expression databases

GenevisibleiQ7XJR9. AT.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDPKG_ARATH
AccessioniPrimary (citable) accession number: Q7XJR9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: October 1, 2003
Last modified: November 30, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.