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Protein

Calcium-dependent protein kinase 16

Gene

CPK16

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei137ATPPROSITE-ProRule annotation1
Active sitei234Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi114 – 122ATPPROSITE-ProRule annotation9
Calcium bindingi424 – 4351PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi461 – 4722PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi503 – 5143PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi533 – 5444PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Calcium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-111932 CaMK IV-mediated phosphorylation of CREB
R-ATH-442745 Activation of CaMK IV
R-ATH-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-ATH-69473 G2/M DNA damage checkpoint

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase 16 (EC:2.7.11.1)
Gene namesi
Name:CPK16
Ordered Locus Names:At2g17890
ORF Names:T13L16.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G17890
TAIRilocus:2827826 AT2G17890

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2G → A: Loss of myristoylation. Loss of plasma membrane localization and relocation to nucleolus and chloroplasts. 1 Publication1
Mutagenesisi4C → A: Drastic reduction of plasma membrane localization and strong increase of nuclear localization. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCurated
ChainiPRO_00003633382 – 571Calcium-dependent protein kinase 16Add BLAST570

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1
Lipidationi4S-palmitoyl cysteine1 Publication1
Modified residuei274PhosphoserineBy similarity1
Modified residuei541PhosphoserineBy similarity1

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ7XJR9
PRIDEiQ7XJR9

PTM databases

iPTMnetiQ7XJR9

Expressioni

Gene expression databases

ExpressionAtlasiQ7XJR9 baseline and differential
GenevisibleiQ7XJR9 AT

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi1656, 1 interactor
STRINGi3702.AT2G17890.1

Structurei

3D structure databases

ProteinModelPortaliQ7XJR9
SMRiQ7XJR9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini108 – 368Protein kinasePROSITE-ProRule annotationAdd BLAST261
Domaini411 – 447EF-hand 1PROSITE-ProRule annotationAdd BLAST37
Domaini448 – 483EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini484 – 525EF-hand 3PROSITE-ProRule annotationAdd BLAST42
Domaini526 – 555EF-hand 4PROSITE-ProRule annotationAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni374 – 404Autoinhibitory domainBy similarityAdd BLAST31

Domaini

There are 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (374-404) inactivates kinase activity under calcium-free conditions (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032 Eukaryota
ENOG410XRMJ LUCA
HOGENOMiHOG000233030
InParanoidiQ7XJR9
KOiK13412
OMAiASMSNVP
OrthoDBiEOG093605DQ
PhylomeDBiQ7XJR9

Family and domain databases

InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF13499 EF-hand_7, 2 hits
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 4 hits
SM00220 S_TKc, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 4 hits
PS50222 EF_HAND_2, 4 hits
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7XJR9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLCFSSAAK SSGHNRSSRN PHPHPPLTVV KSRPPRSPCS FMAVTIQKDH
60 70 80 90 100
RTQPRRNATA KKTPTRHTPP HGKVREKVIS NNGRRHGETI PYGKRVDFGY
110 120 130 140 150
AKDFDHRYTI GKLLGHGQFG YTYVATDKKT GDRVAVKKID KAKMTIPIAV
160 170 180 190 200
EDVKREVKIL QALTGHENVV RFYNAFEDKN SVYIVMELCE GGELLDRILA
210 220 230 240 250
RKDSRYSERD AAVVVRQMLK VAAECHLRGL VHRDMKPENF LFKSTEEDSP
260 270 280 290 300
LKATDFGLSD FIKPGKKFHD IVGSAYYVAP EVLKRRSGPE SDVWSIGVIS
310 320 330 340 350
YILLCGRRPF WDKTEDGIFK EVLKNKPDFR RKPWPTISNS AKDFVKKLLV
360 370 380 390 400
KDPRARLTAA QALSHPWVRE GGDASEIPID ISVLNNMRQF VKFSRLKQFA
410 420 430 440 450
LRALATTLDE EELADLRDQF DAIDVDKNGV ISLEEMRQAL AKDHPWKLKD
460 470 480 490 500
ARVAEILQAI DSNTDGFVDF GEFVAAALHV NQLEEHDSEK WQQRSRAAFE
510 520 530 540 550
KFDIDGDGFI TAEELRMHTG LKGSIEPLLE EADIDNDGKI SLQEFRRLLR
560 570
TASIKSRNVR SPPGYLISRK V
Length:571
Mass (Da):64,754
Last modified:October 1, 2003 - v1
Checksum:i282D5E81EA571D74
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002685 Genomic DNA Translation: AEC06699.1
PIRiT00835
RefSeqiNP_179379.1, NM_127343.2

Genome annotation databases

EnsemblPlantsiAT2G17890.1; AT2G17890.1; AT2G17890
GeneIDi816299
GrameneiAT2G17890.1; AT2G17890.1; AT2G17890
KEGGiath:AT2G17890

Similar proteinsi

Entry informationi

Entry nameiCDPKG_ARATH
AccessioniPrimary (citable) accession number: Q7XJR9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: October 1, 2003
Last modified: May 23, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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