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Protein

Fatty acid amide hydrolase

Gene

FAAH

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Degrades bioactive fatty acid amides to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Converts N-actylethanolamine (NAE) to ethanolamine. Can also use oleamide or 2-arachidonylglycerol as substrates, but not indole-3-acetamide, 1-naphtalene-acetamide, nicotinic acid amide or L-asparagine. Might be involved in abscisic acid signaling and plant defense through distinctly different mechanisms not involving the catalytic activity.4 Publications

Catalytic activityi

Anandamide + H2O = arachidonic acid + ethanolamine.
Oleamide + H2O = oleic acid + NH3.

Enzyme regulationi

Inhibited by methylarachidonyl fluorophosphonate (MAFP).1 Publication

Kineticsi

  1. KM=13.6 µM for N-actylethanolamine 20:41 Publication
  2. KM=26.2 µM for N-actylethanolamine 18:21 Publication
  3. KM=50.8 µM for N-actylethanolamine 16:01 Publication
  4. KM=37.0 µM for N-actylethanolamine 14:01 Publication
  5. KM=17.6 µM for N-actylethanolamine 12:01 Publication
  1. Vmax=17.9 µmol/h/mg enzyme with N-actylethanolamine 20:4 as substrate1 Publication
  2. Vmax=14.1 µmol/h/mg enzyme with N-actylethanolamine 18:2 as substrate1 Publication
  3. Vmax=12.1 µmol/h/mg enzyme with N-actylethanolamine 16:0 as substrate1 Publication
  4. Vmax=9.1 µmol/h/mg enzyme with N-actylethanolamine 14:0 as substrate1 Publication
  5. Vmax=13.9 µmol/h/mg enzyme with N-actylethanolamine 12:0 as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei205Charge relay systemCurated1
Active sitei281Charge relay systemCurated1
Active sitei305Acyl-ester intermediateCurated1

GO - Molecular functioni

GO - Biological processi

  • defense response to bacterium Source: TAIR
  • N-acylethanolamine metabolic process Source: TAIR

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BioCyciARA:AT5G64440-MONOMER.
BRENDAi3.5.1.99. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid amide hydrolase (EC:3.5.1.99)
Alternative name(s):
N-acylethanolamine amidohydrolase
Gene namesi
Name:FAAH
Ordered Locus Names:At5g64440
ORF Names:T12B11.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G64440.
TAIRilocus:2179371. AT5G64440.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei32 – 52HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype and enhanced sensitivity to growth inhibition induced by exogenous N-actylethanolamine.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi205K → A: Loss of activity. 1 Publication1
Mutagenesisi281 – 282SS → AA: Loss of activity. 1 Publication2
Mutagenesisi305S → A: Loss of activity. 1 Publication1
Mutagenesisi307R → A: Loss of activity. 1 Publication1
Mutagenesisi360S → A: No effect. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004140261 – 607Fatty acid amide hydrolaseAdd BLAST607

Proteomic databases

PaxDbiQ7XJJ7.
PRIDEiQ7XJJ7.

PTM databases

iPTMnetiQ7XJJ7.

Expressioni

Tissue specificityi

Expressed in seedlings, flowers, roots, siliques, seeds and leaves. Lower levels in stems.2 Publications

Developmental stagei

Up-regulated during seed germination and early postgerminative seedling growth.1 Publication

Gene expression databases

GenevisibleiQ7XJJ7. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G64440.1.

Structurei

3D structure databases

ProteinModelPortaliQ7XJJ7.
SMRiQ7XJJ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni302 – 305Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the amidase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1211. Eukaryota.
COG0154. LUCA.
HOGENOMiHOG000116697.
InParanoidiQ7XJJ7.
OMAiTLINMID.
OrthoDBiEOG093603Z7.
PhylomeDBiQ7XJJ7.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
InterProiView protein in InterPro
IPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiView protein in Pfam
PF01425. Amidase. 1 hit.
SUPFAMiSSF75304. SSF75304. 1 hit.
PROSITEiView protein in PROSITE
PS00571. AMIDASES. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7XJJ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKYQVMKRA SEVDLSTVKY KAETMKAPHL TGLSFKLFVN LLEAPLIGSL
60 70 80 90 100
IVDYLKKDNG MTKIFRNTVI PEEPMFRPEF PSQEPEHDVV IVGEDESPID
110 120 130 140 150
RLETALKCLP QYDPSRSLHA DPVSSFRYWK IRDYAYAYRS KLTTPLQVAK
160 170 180 190 200
RIISIIEEFG YDKPPTPFLI RFDANEVIKQ AEASTRRFEQ GNPISVLDGI
210 220 230 240 250
FVTIKDDIDC LPHPTNGGTT WLHEDRSVEK DSAVVSKLRS CGAILLGKAN
260 270 280 290 300
MHELGMGTTG NNSNYGTTRN PHDPKRYTGG SSSGSAAIVA AGLCSAALGT
310 320 330 340 350
DGGGSVRIPS ALCGITGLKT TYGRTDMTGS LCEGGTVEII GPLASSLEDA
360 370 380 390 400
FLVYAAILGS SSADRYNLKP SPPCFPKLLS HNGSNAIGSL RLGKYTKWFN
410 420 430 440 450
DVSSSDISDK CEDILKLLSN NHGCKVVEIV VPELEEMRAA HVISIGSPTL
460 470 480 490 500
SSLTPYCEAG KNSKLSYDTR TSFAIFRSFS ASDYIAAQCL RRRLMEYHLN
510 520 530 540 550
IFKDVDVIVT PTTGMTAPVI PPDALKNGET NIQVTTDLMR FVLAANLLGF
560 570 580 590 600
PAISVPVGYD KEGLPIGLQI MGRPWAEATV LGLAAAVEEL APVTKKPAIF

YDILNTN
Length:607
Mass (Da):66,079
Last modified:October 1, 2003 - v1
Checksum:iB659CFA60EFC1633
GO

Sequence cautioni

The sequence AAL09742 differs from that shown. Reason: Frameshift at position 148.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti127R → W in BAB11605 (Ref. 3) Curated1
Sequence conflicti127R → W in AAF73891 (Ref. 1) Curated1
Sequence conflicti571M → R in AAF73891 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF223949 mRNA. Translation: AAF73891.1.
AY308736 mRNA. Translation: AAP83139.1.
AB025640 Genomic DNA. Translation: BAB11605.1.
CP002688 Genomic DNA. Translation: AED97893.1.
AY057501 mRNA. Translation: AAL09742.1. Frameshift.
AY143870 mRNA. Translation: AAN28809.1.
RefSeqiNP_201249.2. NM_125840.4.
UniGeneiAt.19619.

Genome annotation databases

EnsemblPlantsiAT5G64440.1; AT5G64440.1; AT5G64440.
GeneIDi836565.
GrameneiAT5G64440.1; AT5G64440.1; AT5G64440.
KEGGiath:AT5G64440.

Similar proteinsi

Entry informationi

Entry nameiFAAH_ARATH
AccessioniPrimary (citable) accession number: Q7XJJ7
Secondary accession number(s): Q93ZI9, Q9FGF2, Q9LLC0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 16, 2011
Last sequence update: October 1, 2003
Last modified: September 27, 2017
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families