Q7XJE6 (MCA1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Metacaspase-1
Short name=AtMC1
EC=3.4.22.-

Alternative name(s):
Metacaspase 1b
Short name=AtMCP1b
Protein LSD ONE LIKE 3

Gene names

Name:	AMC1
Synonyms:	LOL3, MCP1B
Ordered Locus Names:	At1g02170
ORF Names:	T7I23.17

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cysteine protease that cleaves specifically after arginine or lysine residues. Does not cleave caspase-specific substrates. Acts as a positive regulator of cell death. Required for both oxidative stress cell death response and hypersensitive cell death response mediated by immune response. Ref.6 Ref.7
Subunit structure	Interacts (via N-terminus) with LSD1. Ref.7
Post-translational modification	Proteolytically processed; by an autocatalytic mechanism.
Disruption phenotype	No visible phenotype under normal growth conditions, but suppression of hypersensitive cell death response upon infection with avirulent pathogen. Ref.7
Sequence similarities	Belongs to the peptidase C14B family.
Sequence caution	The sequence AAC24380.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Plant defense
Molecular function	Hydrolase Protease Thiol protease
PTM	Autocatalytic cleavage
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW induction of apoptosis Inferred from direct assay Ref.6. Source: TAIR proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cysteine-type endopeptidase activity Inferred from direct assay Ref.6. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	Metacaspase-1		PRO_0000334599
Regions
	Compositional bias	3 – 76	74	Pro-rich
Sites
	Active site	164	1	By similarity
	Active site	220	1
Experimental info
	Mutagenesis	220	1	C → A: Loss of autoprocessing and cell death regulatory function. Ref.6 Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q7XJE6 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 98DCC44A986E0C5A	FASTA	367	39,792
	10 20 30 40 50 60 MYPPPPSSIY APPMLVNCSG CRTPLQLPSG ARSIRCALCQ AVTHIADPRT APPPQPSSAP 70 80 90 100 110 120 SPPPQIHAPP GQLPHPHGRK RAVICGISYR FSRHELKGCI NDAKCMRHLL INKFKFSPDS 130 140 150 160 170 180 ILMLTEEETD PYRIPTKQNM RMALYWLVQG CTAGDSLVFH YSGHGSRQRN YNGDEVDGYD 190 200 210 220 230 240 ETLCPLDFET QGMIVDDEIN ATIVRPLPHG VKLHSIIDAC HSGTVLDLPF LCRMNRAGQY 250 260 270 280 290 300 VWEDHRPRSG LWKGTAGGEA ISISGCDDDQ TSADTSALSK ITSTGAMTFC FIQAIERSAQ 310 320 330 340 350 360 GTTYGSLLNS MRTTIRNTGN DGGGSGGVVT TVLSMLLTGG SAIGGLRQEP QLTACQTFDV YAKPFTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Type II metacaspases Atmc4 and Atmc9 of Arabidopsis thaliana cleave substrates after arginine and lysine." Vercammen D., van de Cotte B., De Jaeger G., Eeckhout D., Casteels P., Vandepoele K., Vandenberghe I., van Beeumen J., Inze D., van Breusegem F. J. Biol. Chem. 279:45329-45336(2004) [PubMed: 15326173] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE.
[2]	"Characterization of metacaspases." Ikeda Y., Krishnamurthy N., Chua N.-H. Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Recent advance in the study of caspase-like proteases and Bax inhibitor-1 in plants: their possible roles as regulator of programmed cell death." Watanabe N., Lam E. Mol. Plant Pathol. 5:65-70(2004) [PubMed: 20565583] [Abstract] Cited for: GENE FAMILY.
[6]	"Two Arabidopsis metacaspases AtMCP1b and AtMCP2b are arginine/lysine-specific cysteine proteases and activate apoptosis-like cell death in yeast." Watanabe N., Lam E. J. Biol. Chem. 280:14691-14699(2005) [PubMed: 15691845] [Abstract] Cited for: FUNCTION, AUTOCATALYTIC CLEAVAGE, MUTAGENESIS OF CYS-220.
[7]	"Arabidopsis type I metacaspases control cell death." Coll N.S., Vercammen D., Smidler A., Clover C., Van Breusegem F., Dangl J.L., Epple P. Science 330:1393-1397(2010) [PubMed: 21097903] [Abstract] Cited for: FUNCTION, INTERACTION WITH LSD1, DISRUPTION PHENOTYPE, MUTAGENESIS OF CYS-220.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY219826 mRNA. Translation: AAP44514.1. AY322525 mRNA. Translation: AAP84706.1. U89959 Genomic DNA. Translation: AAC24380.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE27396.1.
IPI	IPI00531085.
RefSeq	NP_171719.2. NM_100097.4.
UniGene	At.10711. At.69983.
3D structure databases
ProteinModelPortal	Q7XJE6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7XJE6.
Protein family/group databases
MEROPS	C14.047.
Proteomic databases
PRIDE	Q7XJE6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G02170.1; AT1G02170.1; AT1G02170.
GeneID	839561.
GenomeReviews	Gene locus AT1G02170 in contig CT485782_GR.
KEGG	ath:AT1G02170.
NMPDR	fig|3702.1.peg.334.
Organism-specific databases
TAIR	At1g02170.
Phylogenomic databases
eggNOG	KOG1546.
GeneTree	EPGT00070000028362.
HOGENOM	HBG692861.
InParanoid	Q7XJE6.
OMA	NDAKCMR.
PhylomeDB	Q7XJE6.
Gene expression databases
Genevestigator	Q7XJE6.
Family and domain databases
InterPro	IPR011600. Pept_C14_cat. IPR005735. Znf_LSD1. [Graphical view]
Pfam	PF00656. Peptidase_C14. 1 hit. PF06943. zf-LSD1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01053. LSD1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCA1_ARATH

Accession

Primary (citable) accession number: Q7XJE6
Secondary accession number(s): O81911

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	October 1, 2003
Last modified:	November 16, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program