Reviewed, UniProtKB/Swiss-Prot Q7XJ98 (KATAM_ARATH)

Names and origin

Protein names

Recommended name:
    Xyloglucan galactosyltransferase KATAMARI1
    EC=2.4.1.-
Alternative name(s):
    Protein MURUS 3

Gene names

Name:	KAM1
Synonyms:	MUR3
Ordered Locus Names:	At2g20370
ORF Names:	F11A3.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	619 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the attachment of the Gal residue on the third xylosyl unit within the XXXG core structure of xyloglucan, the principal glycan that interlaces the cellulose microfibrils in plant cell wall. Interacts with actin and is required for the proper endomembrane organization and for the cell elongation. Not involved in the trafficking from the endoplasmic reticulum to the vacuoles. Ref.1 Ref.2
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Ref.2
Tissue specificity	Ubiquitous. Ref.1
Domain	The cytoplasmic N-terminal domain interacts with actin, while the lumenal C-terminal domain contributes to the activity of xyloglucan galactosyltransferase.
Sequence similarities	Belongs to the glycosyltransferase 47 family.
Sequence caution	The sequence AAD21751.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	endomembrane organization Ref.2 Inferred from mutant phenotype. Source: TAIR fucose biosynthetic process Inferred from mutant phenotype. Source: TAIR salicylic acid mediated signaling pathway Inferred from mutant phenotype. Source: TAIR unidimensional cell growth Ref.2 Inferred from mutant phenotype. Source: TAIR xyloglucan biosynthetic process Inferred from mutant phenotype. Source: TAIR
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to Golgi membrane Ref.2 Inferred from direct assay. Source: TAIR
Molecular function	transferase activity, transferring glycosyl groups Ref.1 Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 619	619	Xyloglucan galactosyltransferase KATAMARI1		PRO_0000149667
Regions
	Topological domain	1 – 33	33	Cytoplasmic
	Transmembrane	34 – 54	21	Signal-anchor for type II membrane protein
	Topological domain	55 – 619	565	Lumenal
	Compositional bias	85 – 124	40	Pro-rich
Experimental info
	Mutagenesis	290	1	A → V in mur3-2; altered xyloglucan, but normal endomembrane organization. Ref.1
	Mutagenesis	470	1	S → L in mur3-1; altered xyloglucan, but normal endomembrane organization. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q7XJ98-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 16CB8D880CBC893D	FASTA	619	70,744
	10 20 30 40 50 60 MFPRVSMRRR SAEVSPTEPM EKGNGKNQTN RICLLVALSL FFWALLLYFH FVVLGTSNID 70 80 90 100 110 120 KQLQLQPSYA QSQPSSVSLR VDKFPIEPHA APSKPPPKEP LVTIDKPILP PAPVANSSST 130 140 150 160 170 180 FKPPRIVESG KKQEFSFIRA LKTVDNKSDP CGGKYIYVHN LPSKFNEDML RDCKKLSLWT 190 200 210 220 230 240 NMCKFTTNAG LGPPLENVEG VFSDEGWYAT NQFAVDVIFS NRMKQYKCLT NDSSLAAAIF 250 260 270 280 290 300 VPFYAGFDIA RYLWGYNISR RDAASLELVD WLMKRPEWDI MRGKDHFLVA GRITWDFRRL 310 320 330 340 350 360 SEEETDWGNK LLFLPAAKNM SMLVVESSPW NANDFGIPYP TYFHPAKDSE VFEWQDRMRN 370 380 390 400 410 420 LERKWLFSFA GAPRPDNPKS IRGQIIDQCR NSNVGKLLEC DFGESKCHAP SSIMQMFQSS 430 440 450 460 470 480 LFCLQPQGDS YTRRSAFDSM LAGCIPVFFH PGSAYTQYTW HLPKNYTTYS VFIPEDDVRK 490 500 510 520 530 540 RNISIEERLL QIPAKQVKIM RENVINLIPR LIYADPRSEL ETQKDAFDVS VQAVIDKVTR 550 560 570 580 590 600 LRKNMIEGRT EYDYFVEENS WKYALLEEGQ REAGGHVWDP FFSKPKPGED GSSDGNGGTT 610 ISADAAKNSW KSEQRDKTQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The MUR3 gene of Arabidopsis encodes a xyloglucan galactosyltransferase that is evolutionarily related to animal exostosins." Madson M., Dunand C., Li X., Verma R., Vanzin G.F., Caplan J., Shoue D.A., Carpita N.C., Reiter W.-D. Plant Cell 15:1662-1670(2003) [PubMed: 12837954] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTAGENESIS OF ALA-290 AND SER-470, TISSUE SPECIFICITY.
[2]	"KATAMARI1/MURUS3 is a novel Golgi membrane protein that is required for endomembrane organization in Arabidopsis." Tamura K., Shimada T., Kondo M., Nishimura M., Hara-Nishimura I. Plant Cell 17:1764-1776(2005) [PubMed: 15863516] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY195743 Genomic DNA. Translation: AAO39815.1. AC006569 Genomic DNA. Translation: AAD21751.1. Sequence problems. BT023734 mRNA. Translation: AAZ23926.1.
IPI	IPI00544087.
PIR	D84588.
RefSeq	NP_179627.2.
UniGene	At.39706
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT47. Glycosyltransferase Family 47.
Proteomic databases
PRIDE	Q7XJ98.
Genome annotation databases
GeneID	816556.
GenomeReviews	Gene locus AT2G20370 in contig CT485783_GR.
KEGG	ath:AT2G20370.
NMPDR	fig|3702.1.peg.9032.
Organism-specific databases
TAIR	At2g20370.
Phylogenomic databases
OMA	Q7XJ98. EENSWKY.
Gene expression databases
GermOnline	AT2G20370. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR004263. Exostosin. [Graphical view]
Pfam	PF03016. Exostosin. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATAM_ARATH

Accession

Primary (citable) accession number: Q7XJ98
Secondary accession number(s): Q494P2, Q9SK65

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	October 1, 2003
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)