Xyloglucan galactosyltransferase KATAMARI1 - Arabidopsis thaliana (Mouse-ear cress)

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Q7XJ98 (KATAM_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 62. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Xyloglucan galactosyltransferase KATAMARI1
EC=2.4.1.-

Alternative name(s):
Protein MURUS 3

Gene names

Name:	KAM1
Synonyms:	MUR3
Ordered Locus Names:	At2g20370
ORF Names:	F11A3.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	619 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the attachment of the Gal residue on the third xylosyl unit within the XXXG core structure of xyloglucan, the principal glycan that interlaces the cellulose microfibrils in plant cell wall. Interacts with actin and is required for the proper endomembrane organization and for the cell elongation. Not involved in the trafficking from the endoplasmic reticulum to the vacuoles. Ref.1 Ref.2
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein Ref.2.
Tissue specificity	Ubiquitous. Ref.1
Domain	The cytoplasmic N-terminal domain interacts with actin, while the lumenal C-terminal domain contributes to the activity of xyloglucan galactosyltransferase.
Sequence similarities	Belongs to the glycosyltransferase 47 family.
Sequence caution	The sequence AAD21751.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endomembrane system organization Inferred from mutant phenotype Ref.2. Source: TAIR fucose biosynthetic process Inferred from mutant phenotype PubMed 9301086. Source: TAIR protein glycosylation Inferred from electronic annotation. Source: UniProtKB-UniPathway salicylic acid mediated signaling pathway Inferred from mutant phenotype PubMed 18657237. Source: TAIR unidimensional cell growth Inferred from mutant phenotype Ref.2. Source: TAIR xyloglucan biosynthetic process Inferred from mutant phenotype PubMed 12481058. Source: TAIR
Cellular_component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endosome Inferred from direct assay PubMed 22923678. Source: TAIR integral to Golgi membrane Inferred from direct assay Ref.2. Source: TAIR trans-Golgi network Inferred from direct assay PubMed 22923678. Source: TAIR
Molecular_function	transferase activity, transferring glycosyl groups Inferred from direct assay Ref.1. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 619

619

Xyloglucan galactosyltransferase KATAMARI1

PRO_0000149667

Regions

Topological domain

1 – 33

Cytoplasmic

Transmembrane

34 – 54

Helical; Signal-anchor for type II membrane protein

Topological domain

55 – 619

565

Lumenal

Compositional bias

85 – 124

Pro-rich

Experimental info

Mutagenesis

290

A → V in mur3-2; altered xyloglucan, but normal endomembrane organization. Ref.1

Mutagenesis

470

S → L in mur3-1; altered xyloglucan, but normal endomembrane organization. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q7XJ98 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 16CB8D880CBC893D
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FASTA

619

70,744

        10         20         30         40         50         60 
MFPRVSMRRR SAEVSPTEPM EKGNGKNQTN RICLLVALSL FFWALLLYFH FVVLGTSNID 

        70         80         90        100        110        120 
KQLQLQPSYA QSQPSSVSLR VDKFPIEPHA APSKPPPKEP LVTIDKPILP PAPVANSSST 

       130        140        150        160        170        180 
FKPPRIVESG KKQEFSFIRA LKTVDNKSDP CGGKYIYVHN LPSKFNEDML RDCKKLSLWT 

       190        200        210        220        230        240 
NMCKFTTNAG LGPPLENVEG VFSDEGWYAT NQFAVDVIFS NRMKQYKCLT NDSSLAAAIF 

       250        260        270        280        290        300 
VPFYAGFDIA RYLWGYNISR RDAASLELVD WLMKRPEWDI MRGKDHFLVA GRITWDFRRL 

       310        320        330        340        350        360 
SEEETDWGNK LLFLPAAKNM SMLVVESSPW NANDFGIPYP TYFHPAKDSE VFEWQDRMRN 

       370        380        390        400        410        420 
LERKWLFSFA GAPRPDNPKS IRGQIIDQCR NSNVGKLLEC DFGESKCHAP SSIMQMFQSS 

       430        440        450        460        470        480 
LFCLQPQGDS YTRRSAFDSM LAGCIPVFFH PGSAYTQYTW HLPKNYTTYS VFIPEDDVRK 

       490        500        510        520        530        540 
RNISIEERLL QIPAKQVKIM RENVINLIPR LIYADPRSEL ETQKDAFDVS VQAVIDKVTR 

       550        560        570        580        590        600 
LRKNMIEGRT EYDYFVEENS WKYALLEEGQ REAGGHVWDP FFSKPKPGED GSSDGNGGTT 

       610 
ISADAAKNSW KSEQRDKTQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The MUR3 gene of Arabidopsis encodes a xyloglucan galactosyltransferase that is evolutionarily related to animal exostosins." Madson M., Dunand C., Li X., Verma R., Vanzin G.F., Caplan J., Shoue D.A., Carpita N.C., Reiter W.-D. Plant Cell 15:1662-1670(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTAGENESIS OF ALA-290 AND SER-470, TISSUE SPECIFICITY.
[2]	"KATAMARI1/MURUS3 is a novel Golgi membrane protein that is required for endomembrane organization in Arabidopsis." Tamura K., Shimada T., Kondo M., Nishimura M., Hara-Nishimura I. Plant Cell 17:1764-1776(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Arabidopsis ORF clones." Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY195743 Genomic DNA. Translation: AAO39815.1. AC006569 Genomic DNA. Translation: AAD21751.1. Sequence problems. CP002685 Genomic DNA. Translation: AEC06999.1. BT023734 mRNA. Translation: AAZ23926.1.
IPI	IPI00544087.
PIR	D84588.
RefSeq	NP_179627.2. NM_127596.2.
UniGene	At.39706.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT47. Glycosyltransferase Family 47.
Proteomic databases
PaxDb	Q7XJ98.
PRIDE	Q7XJ98.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G20370.1; AT2G20370.1; AT2G20370.
GeneID	816556.
KEGG	ath:AT2G20370.
Organism-specific databases
TAIR	At2g20370.
Phylogenomic databases
eggNOG	NOG269133.
HOGENOM	HOG000239726.
InParanoid	Q7XJ98.
OMA	EGRTEYD.
PhylomeDB	Q7XJ98.
ProtClustDB	CLSN2690815.
Enzyme and pathway databases
BioCyc	ARA:AT2G20370-MONOMER. MetaCyc:AT2G20370-MONOMER.
UniPathway	UPA00378.
Gene expression databases
ArrayExpress	Q7XJ98.
Genevestigator	Q7XJ98.
GermOnline	AT2G20370. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR004263. Exostosin. [Graphical view]
Pfam	PF03016. Exostosin. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATAM_ARATH

Accession

Primary (citable) accession number: Q7XJ98
Secondary accession number(s): Q494P2, Q9SK65

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	October 1, 2003
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents