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Q7XJ53

- P2C35_ARATH

UniProt

Q7XJ53 - P2C35_ARATH

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Protein
Probable protein phosphatase 2C 35
Gene
At3g06270, F28L1.21
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi93 – 931Manganese 1 By similarity
Metal bindingi93 – 931Manganese 2 By similarity
Metal bindingi94 – 941Manganese 1; via carbonyl oxygen By similarity
Metal bindingi289 – 2891Manganese 2 By similarity
Metal bindingi333 – 3331Manganese 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein serine/threonine phosphatase activity Source: InterPro

GO - Biological processi

  1. protein dephosphorylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT3G06270-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protein phosphatase 2C 35 (EC:3.1.3.16)
Short name:
AtPP2C35
Gene namesi
Ordered Locus Names:At3g06270
ORF Names:F28L1.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G06270.

Subcellular locationi

GO - Cellular componenti

  1. plasma membrane Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 348348Probable protein phosphatase 2C 35
PRO_0000367962Add
BLAST

Proteomic databases

PaxDbiQ7XJ53.
PRIDEiQ7XJ53.

Expressioni

Gene expression databases

GenevestigatoriQ7XJ53.

Interactioni

Protein-protein interaction databases

BioGridi5136. 1 interaction.
IntActiQ7XJ53. 1 interaction.
MINTiMINT-8390749.

Structurei

3D structure databases

ProteinModelPortaliQ7XJ53.
SMRiQ7XJ53. Positions 52-343.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini81 – 304224PP2C-like
Add
BLAST

Sequence similaritiesi

Belongs to the PP2C family.
Contains 1 PP2C-like domain.

Phylogenomic databases

eggNOGiCOG0631.
HOGENOMiHOG000071030.
InParanoidiQ7XJ53.
OMAiKCCSRYP.
PhylomeDBiQ7XJ53.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 2 hits.
PROSITEiPS01032. PP2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7XJ53-1 [UniParc]FASTAAdd to Basket

« Hide

MGCVQCKCCS RYPSSSSDGD SRGPLEANGV LKGKDQKPLG SIHVPSPNFD    50
MVYSVLSQRG YYPDSPDKEN QDTYCIKTEL QGNPNVHFFG VFDGHGVLGT 100
QCSNFVKERV VEMLSEDPTL LEDPEKAYKS AFLRVNEELH DSEIDDSMSG 150
TTAITVLVVG DKIYVANVGD SRAVLAVKDR NRILAEDLSY DQTPFRKDEC 200
ERVKACGARV LSVDQVEGLK DPNIQTWANE ESEGGDPPRL WVQNGMYPGT 250
AFTRSVGDFT AESIGVIAEP EVSMVHLSPN HLFFVVASDG IFEFLPSQAV 300
VDMVGRYADP RDGCAAAAAE SYKLWLEHEN RTDDITIIIV QIKKLSNE 348
Length:348
Mass (Da):38,330
Last modified:October 1, 2003 - v1
Checksum:i2F20E79DC63515B0
GO

Sequence cautioni

The sequence AAF30320.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC018907 Genomic DNA. Translation: AAF30320.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE74367.1.
BT009732 mRNA. Translation: AAP88366.1.
AK227928 mRNA. Translation: BAE99898.1.
RefSeqiNP_187278.2. NM_111502.4.
UniGeneiAt.40545.

Genome annotation databases

EnsemblPlantsiAT3G06270.1; AT3G06270.1; AT3G06270.
GeneIDi819801.
KEGGiath:AT3G06270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC018907 Genomic DNA. Translation: AAF30320.1 . Sequence problems.
CP002686 Genomic DNA. Translation: AEE74367.1 .
BT009732 mRNA. Translation: AAP88366.1 .
AK227928 mRNA. Translation: BAE99898.1 .
RefSeqi NP_187278.2. NM_111502.4.
UniGenei At.40545.

3D structure databases

ProteinModelPortali Q7XJ53.
SMRi Q7XJ53. Positions 52-343.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 5136. 1 interaction.
IntActi Q7XJ53. 1 interaction.
MINTi MINT-8390749.

Proteomic databases

PaxDbi Q7XJ53.
PRIDEi Q7XJ53.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G06270.1 ; AT3G06270.1 ; AT3G06270 .
GeneIDi 819801.
KEGGi ath:AT3G06270.

Organism-specific databases

TAIRi AT3G06270.

Phylogenomic databases

eggNOGi COG0631.
HOGENOMi HOG000071030.
InParanoidi Q7XJ53.
OMAi KCCSRYP.
PhylomeDBi Q7XJ53.

Enzyme and pathway databases

BioCyci ARA:AT3G06270-MONOMER.

Gene expression databases

Genevestigatori Q7XJ53.

Family and domain databases

Gene3Di 3.60.40.10. 1 hit.
InterProi IPR001932. PP2C-like_dom.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view ]
PANTHERi PTHR13832. PTHR13832. 1 hit.
Pfami PF00481. PP2C. 1 hit.
[Graphical view ]
SMARTi SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF81606. SSF81606. 2 hits.
PROSITEi PS01032. PP2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
    Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
    BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiP2C35_ARATH
AccessioniPrimary (citable) accession number: Q7XJ53
Secondary accession number(s): Q9M8J1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: October 1, 2003
Last modified: June 11, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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