Reviewed, UniProtKB/Swiss-Prot Q7XJ02 (APX7_ORYSJ)

Names and origin

Protein names

Recommended name:
    Probable L-ascorbate peroxidase 7, chloroplastic
    EC=1.11.1.11
Alternative name(s):
    OsAPx07

Gene names

Name:	APX7
Ordered Locus Names:	Os04g0434800, LOC_Os04g35520
ORF Names:	OSJNBb0086G13.10

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a key role in hydrogen peroxide removal By similarity.
Catalytic activity	L-ascorbate + H(2)O(2) = dehydroascorbate + 2 H(2)O.
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group By similarity. Binds 1 potassium or calcium ion per subunit By similarity.
Subcellular location	Plastid › chloroplast stromaProbable.
Sequence similarities	Belongs to the peroxidase family. Ascorbate peroxidase subfamily.
Sequence caution	The sequence CAD41021.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Calcium Heme Iron Metal-binding Potassium
Molecular function	Oxidoreductase Peroxidase
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-ascorbate peroxidase activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 71	71	Chloroplast Potential
	Chain	72 – 359	288	Probable L-ascorbate peroxidase 7, chloroplastic		PRO_0000042657
Regions
	Compositional bias	2 – 95	94	Ala-rich
Sites
	Active site	118	1	Proton acceptor By similarity
	Metal binding	247	1	Iron (heme axial ligand)
	Metal binding	248	1	Potassium or calcium By similarity
	Metal binding	280	1	Potassium or calcium By similarity
	Metal binding	287	1	Potassium or calcium By similarity
	Site	114	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7XJ02-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: AB4AA8063826E93B	FASTA	359	38,325
	10 20 30 40 50 60 MAAQRLAALH AAAPSAFSST SSASHGRPAA RSSTTALLPV ALPRASATLR AAPSRLLPQE 70 80 90 100 110 120 AKAAGSGRSV MCMASASASA ASAAVASGAA ELKAAREDIR ELLKTTHCHP ILVRLGWHDS 130 140 150 160 170 180 GTYDKNIKEW PQRGGANGSL RFDVELKHGA NAGLVNALKL VQPIKDKYPN ISYADLFQLA 190 200 210 220 230 240 SATAIEEAGG PKIPMTYGRI DVTGPEQCPP EGKLPDAGPS APADHLRKVF YRMGLDDKEI 250 260 270 280 290 300 VVLSGAHTLG RSRPERSGWG KPETKYTKNG PGAPGGQSWT AEWLKFDNSY FKEIKEKRDQ 310 320 330 340 350 DLLVLPTDAA LFEDPTFKVY AEKYAEDQEA FFKDYAGAHA KLSNLGAKFN PPEGFTLDG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The expression of rice chloroplastic ascorbate peroxidase genes during greening." Morita S., Kotani T., Kaminaka H., Masumura T., Tanaka K. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare.
[2]	"Sequence and analysis of rice chromosome 4." Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y., Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q., Zhang L. , Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T., Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R., Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G., Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C., Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J., Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J., Li J., Hong G., Xue Y., Han B. Nature 420:316-320(2002) [PubMed: 12447439] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Analysis of the molecular evolutionary history of the ascorbate peroxidase gene family: inferences from the rice genome." Teixeira F.K., Menezes-Benavente L., Margis R., Margis-Pinheiro M. J. Mol. Evol. 59:761-770(2004) [PubMed: 15599508] [Abstract] Cited for: NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB114855 mRNA. Translation: BAC79362.1. AL606706 Genomic DNA. Translation: CAD41021.1. Sequence problems.
RefSeq	NP_001052844.1.
UniGene	Os.26502
3D structure databases
HSSP	HSSP built from PDB template 1IYN based on UniProtKB Q8LNY5.
SMR	Q7XJ02. Positions 86-359.
ModBase	Search...
Protein family/group databases
PeroxiBase	1871. OsAPx07.
Genome annotation databases
GeneID	4335896.
KEGG	osa:4335896.
Organism-specific databases
Gramene	Q7XJ02.
Family and domain databases
InterPro	IPR002207. Asc_perxdse. IPR002016. Haem_peroxidase_pln/fun/bac. [Graphical view]
Pfam	PF00141. peroxidase. 1 hit. [Graphical view]
PRINTS	PR00459. ASPEROXIDASE. PR00458. PEROXIDASE.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. False negative. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

APX7_ORYSJ

Accession

Primary (citable) accession number: Q7XJ02
Secondary accession number(s): Q7XUS8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	October 1, 2003
Last modified:	November 25, 2008
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)