Q7XI75 (CARM1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable histone-arginine methyltransferase CARM1
EC=2.1.1.-
EC=2.1.1.125

Alternative name(s):
Protein arginine N-methyltransferase 4

Gene names

Name:	CARM1
Synonyms:	PRMT4
Ordered Locus Names:	Os07g0671700, LOC_Os07g47500
ORF Names:	P0470D12.124

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	528 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, and mRNA stability. Recruited to promoters upon gene activation, methylates histone H3 and activates transcription via chromatin remodeling By similarity.
Catalytic activity	S-adenosyl-L-methionine + arginine-[histone] = S-adenosyl-L-homocysteine + N(omega)-methyl-arginine-[histone].
Subcellular location	Nucleus By similarity. Cytoplasm By similarity.
Sequence similarities	Belongs to the protein arginine N-methyltransferase family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Transferase
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	histone-arginine N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 528	528	Probable histone-arginine methyltransferase CARM1		PRO_0000294007
Regions
	Compositional bias	496 – 517	22	Gln-rich
Sites
	Binding site	166	1	S-adenosyl-L-methionine By similarity
	Binding site	175	1	S-adenosyl-L-methionine By similarity
	Binding site	199	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	221	1	S-adenosyl-L-methionine By similarity
	Binding site	251	1	S-adenosyl-L-methionine By similarity
	Binding site	279	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7XI75 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 06043252B82901AD	FASTA	528	58,580
	10 20 30 40 50 60 MASPDLFPNV SFSHVSVPAA AGASTEVTGG ATAVFGGDAS TGAPRLSLVW SGETQAKHTL 70 80 90 100 110 120 EIDLSDAQIF KLGPTEWLCV SGESEAKDGV EEKSYSRAIK VVLRTEAESK AFYLAFQQWK 130 140 150 160 170 180 HRVISGKAGE PLENGLIIGS KSKFDTKIEA SSAKMYFHYY GQLLHQQNML QDFVRTGTYY 190 200 210 220 230 240 AAVMENRSDF EGRVVVDVGA GSGILSLFAA QAGARHVYAV EASEMAEHAQ RLISGNPSLG 250 260 270 280 290 300 QRITVIKGKV EEVELPEKAD ILISEPMGTL LVNERMLESY VIARDRFLVP GGKMFPTTGR 310 320 330 340 350 360 IHMAPFSDEY LYVEMANKAL FWQQHNFFGV DLTPLHGSAF QGYFSQPVVD AFDPRLLVSP 370 380 390 400 410 420 PTFHTLDFTT MKEEELYEID IPLNFVASVG TRVHGLACWF DVLFNGSTVQ RWLTTAPGSP 430 440 450 460 470 480 TTHWYQLRCI LSQPLYVMAG QEITGRLHLV AHSAQSYTIY LTMSAKMWGE GAEQGGILQT 490 500 510 520 STAKLELKEP YYRLSQPQPY VMQQDQQQQQ LPSLQPQSPL WDYHYGQD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed: 18089549] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP004300 Genomic DNA. Translation: BAC79844.1. AP008213 Genomic DNA. Translation: BAF22514.1. CM000144 Genomic DNA. No translation available. AK065980 mRNA. No translation available.
RefSeq	NP_001060600.1. NM_001067135.1.
UniGene	Os.10778.
3D structure databases
HSSP	HSSP built from PDB template 1OR8 based on UniProtKB Q63009.
ProteinModelPortal	Q7XI75.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7XI75.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os07g47500.2; LOC_Os07g47500.2; LOC_Os07g47500. LOC_Os07g47500.3; LOC_Os07g47500.3; LOC_Os07g47500.
GeneID	4344248.
KEGG	osa:4344248.
Organism-specific databases
Gramene	Q7XI75.
Phylogenomic databases
GeneTree	EPGT00070000029771.
HOGENOM	HBG717391.
PhylomeDB	Q7XI75.
ProtClustDB	CLSN2690752.
Family and domain databases
InterPro	IPR007857. Skb1_MeTrfase. [Graphical view]
KO	K05931.
Pfam	PF05185. PRMT5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARM1_ORYSJ

Accession

Primary (citable) accession number: Q7XI75
Secondary accession number(s): A3BNA6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	October 1, 2003
Last modified:	November 16, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program