Reviewed, UniProtKB/Swiss-Prot Q7XE50 (LAC16_ORYSJ)

Names and origin

Protein names

Recommended name:
    Putative laccase-16
    EC=1.10.3.2
Alternative name(s):
    Benzenediol:oxygen oxidoreductase 16
    Urishiol oxidase 16
    Diphenol oxidase 16

Gene names

Name:	LAC16
Ordered Locus Names:	Os10g0437400, LOC_Os10g30140

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Uncertain.

General annotation (Comments)

Function	Lignin degradation and detoxification of lignin-derived products By similarity.
Catalytic activity	4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O.
Cofactor	Binds 4 copper ions per monomer By similarity.
Subcellular location	Secreted › extracellular space › apoplastPotential.
Sequence similarities	Belongs to the multicopper oxidase family. Contains 3 plastocyanin-like domains.
Caution	Lacks the signal peptide, which is one of the conserved features of the laccases. Could be the product of a pseudogene.

Ontologies

Keywords
Biological process	Lignin degradation
Cellular component	Apoplast Secreted
Domain	Repeat
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	copper ion binding Inferred from electronic annotation. Source: InterPro laccase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 467	467	Putative laccase-16		PRO_0000291900
Regions
	Domain	7 – 88	82	Plastocyanin-like 1
	Domain	98 – 225	128	Plastocyanin-like 2
	Domain	318 – 451	134	Plastocyanin-like 3
Sites
	Metal binding	22	1	Copper 1 By similarity
	Metal binding	24	1	Copper 2 By similarity
	Metal binding	67	1	Copper 2 By similarity
	Metal binding	69	1	Copper 3 By similarity
	Metal binding	368	1	Copper 4 By similarity
	Metal binding	371	1	Copper 1 By similarity
	Metal binding	373	1	Copper 3 By similarity
	Metal binding	430	1	Copper 3 By similarity
	Metal binding	431	1	Copper 4 By similarity
	Metal binding	432	1	Copper 2 By similarity
	Metal binding	436	1	Copper 4 By similarity
	Metal binding	441	1	Copper 4 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7XE50-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 93F9411A5D6E1C21	FASTA	467	52,187
	10 20 30 40 50 60 MDRKGIVLGS KLAVFSMILL WHRHGVDQPR NPWSDGPEFI TQCPIRPCGN FTYQVILFEE 70 80 90 100 110 120 EGTLWWHAHS DFDRATVHGA IVIHPKHGTT FPFNKPDKEI PIILSEWWND DVENVLDEAK 130 140 150 160 170 180 RTGGDQGNTY LLRVINTGLT NDMFFAVAGH CLTVVSIDAR YTKPLTVDYI MIAPGQTMDV 190 200 210 220 230 240 LLEANRTLGS NSRYYMAARA FITLPVDTIP FNNSTATAIV EYTDSPTARP PGPPEFPLLL 250 260 270 280 290 300 PAIKDEDAAM AFVDERMLID IDVNFLPCDT TNATNKLCKG PQGNQFAASL NNVSFESPAI 310 320 330 340 350 360 DVLDAYYYGS GRGVYEEDFP NKPVNAFVNP TGDNGGRPLL TKRGTKVKVV EYGTVVEVVF 370 380 390 400 410 420 QDLSSENHPM HLHGFAFYVV GRGSGTFDER RDPATYNLVD PPFQNTVSVP KSSWAAIRFR 430 440 450 460 ADNPGVWFMH CHFDRHVVWG MDTVFIVKDG KTPQAQMLPR PPNMPEC

References

[1]

"In-depth view of structure, activity, and evolution of rice chromosome 10." Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S., Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D., Oates R., Palmer M., Pries G., Gibson J., Anderson H. , Paradkar M., Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F., Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M., Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R., Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F., Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D., Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R., Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K., Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S., Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S., Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R., Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M., Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R., Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E., Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A. Science 300:1566-1569(2003) [PubMed: 12791992] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DP000086 Genomic DNA. Translation: AAP53940.1.
3D structure databases
HSSP	HSSP built from PDB template 1AOZ based on UniProtKB P37064.
ModBase	Search...
Genome annotation databases
NMPDR	fig|39947.1.peg.13165.
Organism-specific databases
Gramene	Q7XE50.
Family and domain databases
InterPro	IPR001117. Cu-oxidase. IPR011706. Cu-oxidase_2. IPR011707. Cu-oxidase_3. IPR002355. Cu_oxidase_Cu_BS. IPR008972. Cupredoxin. IPR002035. VWF_A. [Graphical view]
Gene3D	G3DSA:2.60.40.420. Cupredoxin. 3 hits.
Pfam	PF00394. Cu-oxidase. 1 hit. PF07731. Cu-oxidase_2. 1 hit. PF07732. Cu-oxidase_3. 1 hit. [Graphical view]
PRINTS	PR00453. VWFADOMAIN.
PROSITE	PS00079. MULTICOPPER_OXIDASE1. 1 hit. PS00080. MULTICOPPER_OXIDASE2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LAC16_ORYSJ

Accession

Primary (citable) accession number: Q7XE50

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	October 1, 2003
Last modified:	November 25, 2008
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)