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Q7XDC8 (MDHC_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase, cytoplasmic

EC=1.1.1.37
Alternative name(s):
PP37
Gene names
Ordered Locus Names:Os10g0478200, LOC_Os10g33800
ORF Names:OSJNBa0055P24.3
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length332 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH.

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity.

Tissue specificity

Highly expressed in young panicles and immature seeds. Weakly expressed in roots and leaves. Expressed in stem and sheath (at protein level). Ref.1 Ref.5

Post-translational modification

Phosphorylated after gibberellin treatment. Ref.6

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 2 family.

Sequence caution

The sequence ABG66141.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
   PTMPhosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

response to cadmium ion

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to salt stress

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to zinc ion

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentapoplast

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

chloroplast stroma

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

cytosol

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

nucleus

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

plasma membrane

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

plasmodesma

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

vacuolar membrane

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Molecular_functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.5
Chain2 – 332331Malate dehydrogenase, cytoplasmic
PRO_0000113418

Regions

Nucleotide binding12 – 187NAD By similarity
Nucleotide binding130 – 1323NAD By similarity

Sites

Active site1881Proton acceptor By similarity
Binding site931Substrate carboxyl By similarity
Binding site991Substrate carboxyl By similarity
Binding site1061NAD By similarity
Binding site1131NAD By similarity
Binding site1321Substrate By similarity
Binding site1631Substrate carboxyl By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7XDC8 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: EB1E3187DCC4DF42

FASTA33235,569
        10         20         30         40         50         60 
MAKEPMRVLV TGAAGQIGYA LVPMIARGVM LGADQPVILH MLDIPPATES LNGLKMELVD 

        70         80         90        100        110        120 
AAFPLLKGIV ATTDVVEACT GVNVAVMVGG FPRKEGMERK DVMSKNVSIY KSQASALEAH 

       130        140        150        160        170        180 
AAPNCKVLVV ANPANTNALI LKEFAPSIPE KNITCLTRLD HNRALGQISE KLNVQVTDVK 

       190        200        210        220        230        240 
NAIIWGNHSS TQYPDVNHAT VKTPSGEKPV RELVADDEWL NTEFISTVQQ RGAAIIKARK 

       250        260        270        280        290        300 
QSSALSAASS ACDHIRDWVL GTPEGTFVSM GVYSDGSYGV PAGLIYSFPV TCSGGEWTIV 

       310        320        330 
QGLPIDEFSR KKMDATAQEL SEEKTLAYSC LN 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, characterization and prokaryotic expression of cytosolic malate dehydrogenase from Oryza sativa."
Lin C.F., Jiang R.H., Jiang L.Z., Qian X.Y., Attia K., Yang J.S.
DNA Seq. 15:314-318(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[2]"In-depth view of structure, activity, and evolution of rice chromosome 10."
Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S., Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D., Oates R., Palmer M., Pries G., Gibson J., Anderson H. expand/collapse author list , Paradkar M., Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F., Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M., Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R., Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F., Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D., Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R., Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K., Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S., Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S., Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R., Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M., Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R., Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E., Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A.
Science 300:1566-1569(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[4]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[5]"Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003."
Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K.
Nucleic Acids Res. 32:D388-D392(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-11; 41-50; 144-153 AND 151-160, TISSUE SPECIFICITY.
Strain: cv. Nipponbare.
Tissue: Panicle, Sheath and Stem.
[6]"Identification of phosphoproteins regulated by gibberellin in rice leaf sheath."
Khan M.M.K., Jan A., Karibe H., Komatsu S.
Plant Mol. Biol. 58:27-40(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INDUCTION, PHOSPHORYLATION.
Strain: cv. Nipponbare.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF353203 mRNA. Translation: AAK26431.1.
AC037425 Genomic DNA. Translation: AAG13573.1.
DP000086 Genomic DNA. Translation: AAP54283.1.
DP000086 Genomic DNA. Translation: ABG66141.1. Sequence problems.
AP008216 Genomic DNA. Translation: BAF26774.1.
RefSeqNP_001064860.1. NM_001071395.1.
UniGeneOs.21850.

3D structure databases

ProteinModelPortalQ7XDC8.
SMRQ7XDC8. Positions 4-332.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ7XDC8.
PRIDEQ7XDC8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS10T0478200-01; OS10T0478200-01; OS10G0478200.
GeneID4348905.
KEGGosa:4348905.

Organism-specific databases

GrameneQ7XDC8.

Phylogenomic databases

eggNOGCOG0039.
HOGENOMHOG000220953.
KOK00025.
OMANCLIASK.
ProtClustDBPLN00135.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR23382. PTHR23382. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMSSF56327. SSF56327. 1 hit.
TIGRFAMsTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMDHC_ORYSJ
AccessionPrimary (citable) accession number: Q7XDC8
Secondary accession number(s): Q0IWZ1, Q109J3, Q9FWH5
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 82 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations