Reviewed,
UniProtKB/Swiss-Prot Q7XDC8 (MDHC_ORYSJ)
Last modified
November 25, 2008.
Version 44.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Malate dehydrogenase, cytoplasmic EC=1.1.1.37 Alternative name(s): PP37 | ||||
| Gene names |
| ||||
| Organism | Oryza sativa subsp. japonica (Rice) | ||||
| Taxonomic identifier | 39947 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza |
Protein attributes
| Sequence length | 332 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | (S)-malate + NAD(+) = oxaloacetate + NADH. |
| Subunit structure | Homodimer By similarity. |
| Subcellular location | CytoplasmBy similarity. |
| Tissue specificity | Highly expressed in young panicles and immature seeds. Weakly expressed in roots and leaves. Expressed in stem and sheath (at protein level). |
| Post-translational modification | Phosphorylated after gibberellin treatment. |
| Sequence similarities | Belongs to the LDH/MDH superfamily. MDH type 2 family. |
| Sequence caution | The sequence ABG66141.1 differs from that shown. Reason: Erroneous gene model prediction. |
Ontologies
Keywords | |
|---|---|
| Biological process | Tricarboxylic acid cycle |
| Cellular component | Cytoplasm |
| Ligand | NAD |
| Molecular function | Oxidoreductase |
| PTM | Phosphoprotein |
| Technical term | Direct protein sequencing |
Gene Ontology (GO) | |
| Biological process | glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic processInferred from electronic annotation. Source: InterPro oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycleInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | L-malate dehydrogenase activity Inferred from electronic annotation. Source: InterPro bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed | ||||||
| Chain | 2 – 332 | 331 | Malate dehydrogenase, cytoplasmic | PRO_0000113418 | |||||
Regions | |||||||||
| Nucleotide binding | 12 – 18 | 7 | NAD By similarity | ||||||
| Nucleotide binding | 130 – 132 | 3 | NAD By similarity | ||||||
Sites | |||||||||
| Active site | 188 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 93 | 1 | Substrate carboxyl By similarity | ||||||
| Binding site | 99 | 1 | Substrate carboxyl By similarity | ||||||
| Binding site | 106 | 1 | NAD By similarity | ||||||
| Binding site | 113 | 1 | NAD By similarity | ||||||
| Binding site | 132 | 1 | Substrate By similarity | ||||||
| Binding site | 163 | 1 | Substrate carboxyl By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning, characterization and prokaryotic expression of cytosolic malate dehydrogenase from Oryza sativa." Lin C.F., Jiang R.H., Jiang L.Z., Qian X.Y., Attia K., Yang J.S. DNA Seq. 15:314-318(2004) [PubMed: 15620222] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. |
| [2] | "In-depth view of structure, activity, and evolution of rice chromosome 10." Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S., Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D., Oates R., Palmer M., Pries G., Gibson J., Anderson H. Dean R.A.Science 300:1566-1569(2003) [PubMed: 12791992] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare. |
| [3] | "Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003." Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K. Nucleic Acids Res. 32:D388-D392(2004) [PubMed: 14681440] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-11; 41-50; 144-153 AND 151-160, TISSUE SPECIFICITY. Strain: cv. Nipponbare. Tissue: Panicle, Sheath and Stem. |
| [4] | "Identification of phosphoproteins regulated by gibberellin in rice leaf sheath." Khan M.M.K., Jan A., Karibe H., Komatsu S. Plant Mol. Biol. 58:27-40(2005) [PubMed: 16028114] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INDUCTION, PHOSPHORYLATION. Strain: cv. Nipponbare. |
Cross-references
Sequence databases | |
|---|---|
| AF353203 mRNA. Translation: AAK26431.1. AC037425 Genomic DNA. Translation: AAG13573.1. AP008216 Genomic DNA. Translation: BAF26774.1. DP000086 Genomic DNA. Translation: AAP54283.1. DP000086 Genomic DNA. Translation: ABG66141.1. Sequence problems. | |
| RefSeq | NP_001064860.1. |
| UniGene | Os.21850 |
3D structure databases | |
| HSSP | HSSP built from PDB template 4MDH based on UniProtKB P11708. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 4348905. |
| KEGG | osa:4348905. |
| NMPDR | fig|39947.1.peg.13505. |
Organism-specific databases | |
| Gramene | Q7XDC8. |
Family and domain databases | |
| InterPro | IPR001557. L-lactate/malate_DHase. IPR001236. Lactate/malate_DHase. IPR015955. Lactate_DHase/Glyco_Ohase_4_C. IPR001252. Malate_DHase_AS. IPR011274. Malate_DHase_NAD-dep_euk. IPR010945. Malate_DHase_SF1. IPR016040. NAD(P)-bd. [Graphical view] |
| Gene3D | G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| PANTHER | PTHR23382. MDH_SF1. 1 hit. |
| Pfam | PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view] |
| PIRSF | PIRSF000102. Lac_mal_DH. 1 hit. |
| ProDom | PD003052. Mal_dehydrog. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| TIGRFAMs | TIGR01759. MalateDH-SF1. 1 hit. TIGR01758. MDH_euk_cyt. 1 hit. |
| PROSITE | PS00068. MDH. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MDHC_ORYSJ | ||||||||
| Accession | Primary (citable) accession number: Q7XDC8 Secondary accession number(s): Q0IWZ1, Q109J3, Q9FWH5 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Oryza sativa (rice) Index of Oryza sativa entries and their corresponding gene designations |
| UniProtKB secondary accession numbers Index of UniProtKB secondary accession numbers |
| SIMILARITY comments Index of protein domains and families |

Clusters with


