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Protein

Protein S-acyltransferase 10

Gene

PAT10

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

S-acyltransferase involved in protein lipid modification. Catalyzes the palmitoylation of proteins peripheral or integral to the tonoplast. Required for the tonoplast localization of CBL2, CBL3 and CBL6, but not for the plasma membrane localization of CBL9, for the endosome localization of RABF1 or for the endomembrane localization of RABF2B.3 Publications

Catalytic activityi

Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei192S-palmitoyl cysteine intermediateBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.3.1.225. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein S-acyltransferase 10 (EC:2.3.1.225)
Alternative name(s):
Probable palmitoyltransferase At3g51390
Zinc finger DHHC domain-containing protein At3g51390
Gene namesi
Name:PAT10
Ordered Locus Names:At3g51390
ORF Names:F26O13.30
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRilocus:2081795. AT3G51390.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei34 – 54HelicalSequence analysisAdd BLAST21
Transmembranei66 – 86HelicalSequence analysisAdd BLAST21
Transmembranei207 – 227HelicalSequence analysisAdd BLAST21
Transmembranei241 – 261HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • vacuolar membrane Source: TAIR

Keywords - Cellular componenti

Membrane, Vacuole

Pathology & Biotechi

Disruption phenotypei

Pleiotropic growth defects, including smaller leaves, dwarfism, and sterility. Hypersensitivity to salt stress and compromised pollen tube growth.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi192C → S: Loss of function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003635971 – 340Protein S-acyltransferase 10Add BLAST340

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiQ7XA86.

PTM databases

iPTMnetiQ7XA86.
SwissPalmiQ7XA86.

Expressioni

Tissue specificityi

Expressed in mature embryos, embryo sacs, cotyledons, whole seedlings, hydathodes, guard cells, sites of lateral root initiation, root tips and phloem, but not in xylem.1 Publication

Developmental stagei

Expressd in tapetal layer of developing anthers and then expression gradually increases in developing microspores and in mature pollen.1 Publication

Inductioni

Not regulated by salt stresses.1 Publication

Gene expression databases

ExpressionAtlasiQ7XA86. baseline and differential.
GenevisibleiQ7XA86. AT.

Interactioni

Protein-protein interaction databases

BioGridi9620. 1 interactor.
IntActiQ7XA86. 1 interactor.
STRINGi3702.AT3G51390.1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini162 – 212DHHCPROSITE-ProRule annotationAdd BLAST51

Domaini

The DHHC domain is required for palmitoyltransferase activity.By similarity

Sequence similaritiesi

Belongs to the DHHC palmitoyltransferase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IREC. Eukaryota.
COG5273. LUCA.
HOGENOMiHOG000030117.
InParanoidiQ7XA86.
KOiK18932.
OMAiWWYICEE.
OrthoDBiEOG09360EGH.
PhylomeDBiQ7XA86.

Family and domain databases

InterProiView protein in InterPro
IPR001594. Palmitoyltrfase_DHHC.
PfamiView protein in Pfam
PF01529. zf-DHHC. 1 hit.
PROSITEiView protein in PROSITE
PS50216. DHHC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7XA86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVCCPFLQP WDRARDQCLL NLPCLSDPVR RSSLLLKLAL VALHLVFIGF
60 70 80 90 100
LFLFDAEFIE KTKRDPWYMG CYILLFSATL LQYFVTSGSS PGYVVDAMRD
110 120 130 140 150
VCEASAMYRN PSTTSIQHAS RKSESVVVNV EGGSASCPRR PPTPWGKLVL
160 170 180 190 200
DLYPPGTSIR NLTCGYCHVE QPPRTKHCHD CDRCVLQFDH HCVWLGTCIG
210 220 230 240 250
QKNHSKFWWY ICEETTLCIW TLIMYVDYLS NVAKPWWKNA IIILLLVILA
260 270 280 290 300
ISLIFVLLLL IFHSYLILTN QSTYELVRRR RIPYMRNIPG RVHPFSRGIR
310 320 330 340
RNLYNVCCGN YNLDSLPTAF ELEDRSRPYT CIDMLKCRCC
Length:340
Mass (Da):39,228
Last modified:October 1, 2003 - v1
Checksum:i029BA53613D8F4E7
GO

Sequence cautioni

The sequence CAB63003 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti140R → K in AAM65117 (Ref. 4) Curated1
Sequence conflicti196G → V in AAM65117 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL133452 Genomic DNA. Translation: CAB63003.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE78787.1.
BT010141 mRNA. Translation: AAQ22610.1.
AY087575 mRNA. Translation: AAM65117.1.
PIRiT45770.
RefSeqiNP_566950.1. NM_114998.5.
UniGeneiAt.35395.
At.75576.

Genome annotation databases

EnsemblPlantsiAT3G51390.1; AT3G51390.1; AT3G51390.
GeneIDi824302.
GrameneiAT3G51390.1; AT3G51390.1; AT3G51390.
KEGGiath:AT3G51390.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL133452 Genomic DNA. Translation: CAB63003.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE78787.1.
BT010141 mRNA. Translation: AAQ22610.1.
AY087575 mRNA. Translation: AAM65117.1.
PIRiT45770.
RefSeqiNP_566950.1. NM_114998.5.
UniGeneiAt.35395.
At.75576.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi9620. 1 interactor.
IntActiQ7XA86. 1 interactor.
STRINGi3702.AT3G51390.1.

PTM databases

iPTMnetiQ7XA86.
SwissPalmiQ7XA86.

Proteomic databases

PaxDbiQ7XA86.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G51390.1; AT3G51390.1; AT3G51390.
GeneIDi824302.
GrameneiAT3G51390.1; AT3G51390.1; AT3G51390.
KEGGiath:AT3G51390.

Organism-specific databases

AraportiAT3G51390.
TAIRilocus:2081795. AT3G51390.

Phylogenomic databases

eggNOGiENOG410IREC. Eukaryota.
COG5273. LUCA.
HOGENOMiHOG000030117.
InParanoidiQ7XA86.
KOiK18932.
OMAiWWYICEE.
OrthoDBiEOG09360EGH.
PhylomeDBiQ7XA86.

Enzyme and pathway databases

BRENDAi2.3.1.225. 399.

Miscellaneous databases

PROiPR:Q7XA86.

Gene expression databases

ExpressionAtlasiQ7XA86. baseline and differential.
GenevisibleiQ7XA86. AT.

Family and domain databases

InterProiView protein in InterPro
IPR001594. Palmitoyltrfase_DHHC.
PfamiView protein in Pfam
PF01529. zf-DHHC. 1 hit.
PROSITEiView protein in PROSITE
PS50216. DHHC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiZDH11_ARATH
AccessioniPrimary (citable) accession number: Q7XA86
Secondary accession number(s): Q8LAV8, Q9SD10
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: October 1, 2003
Last modified: April 12, 2017
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.