Q7XA61 (TIP21_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable aquaporin TIP2-1

Alternative name(s):
Tonoplast intrinsic protein 2-1
Short name=OsTIP2;1

Gene names

Name:	TIP2-1
Synonyms:	TIP2, W950ERIPDM
Ordered Locus Names:	Os02g0658100, LOC_Os02g44080
ORF Names:	OJ1112_F09.18, OsJ_007553, P0519E06.48

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Aquaporins facilitate the transport of water and small neutral solutes across cell membranes. May be involved in transport from the vacuolar compartment to the cytoplasm By similarity.
Subcellular location	Vacuole membrane; Multi-pass membrane protein By similarity. Note: Tonoplast.
Tissue specificity	Expressed in roots and anthers. Ref.7
Induction	Circadian-regulation. Expression is higher during the light phase than during the dark phase. Ref.7
Domain	Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Sequence similarities	Belongs to the MIP/aquaporin (TC 1.A.8) family. TIP (TC 1.A.8.10) subfamily. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane Vacuole
Domain	Repeat Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	methylammonium transmembrane transport Inferred from electronic annotation. Source: GOC
Cellular_component	central vacuole Inferred from electronic annotation. Source: EnsemblPlants/Gramene chloroplast Inferred from electronic annotation. Source: EnsemblPlants/Gramene integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plant-type vacuole membrane Inferred from electronic annotation. Source: EnsemblPlants/Gramene plasma membrane Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	ammonia transmembrane transporter activity Inferred from electronic annotation. Source: EnsemblPlants/Gramene methylammonium transmembrane transporter activity Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	Probable aquaporin TIP2-1		PRO_0000064023
Regions
	Transmembrane	20 – 40	21	Helical; Name=1; Potential
	Transmembrane	54 – 74	21	Helical; Name=2; Potential
	Transmembrane	97 – 119	23	Helical; Name=3; Potential
	Transmembrane	141 – 161	21	Helical; Name=4; Potential
	Transmembrane	168 – 188	21	Helical; Name=5; Potential
	Transmembrane	217 – 237	21	Helical; Name=6; Potential
	Motif	83 – 85	3	NPA 1
	Motif	196 – 198	3	NPA 2

Sequences

	Sequence		Length	Mass (Da)
	Q7XA61 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: F8407683CF23BF3A	FASTA	248	24,878
	10 20 30 40 50 60 MVKLAFGSLG DSFSATSVKA YVAEFIATLL FVFAGVGSAI AYGQLTNGGA LDPAGLVAIA 70 80 90 100 110 120 IAHALALFVG VSVAANISGG HLNPAVTFGL AVGGHITILT GLFYWIAQLL GASIACLLLK 130 140 150 160 170 180 FVTHGKAIPT HGVAGISELE GVVMEIVITF ALVYTVYATA ADPKKGSLGT IAPIAIGFIV 190 200 210 220 230 240 GANILAAGPF SGGSMNPARS FGPAVAAGNF AGNWVYWVGP LIGGGLAGLV YGDVFIGSYQ PVADQDYA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization and expression analysis of tonoplast intrinsic protein isoforms from rice." Takahashi H., Morita S., Masumura T., Tanaka K. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare.
[2]	"Conservation of microstructure bewtween a sequenced region of the genome of rice and multiple segments of the genome of Arabidopsis thaliana." Mayer K., Murphy G., Tarchini R., Wambutt R., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Lemcke K., Haase D., Hall C.R., van Dodeweerd A.-M., Tingey S.V., Mewes H.-W., Bevan M.W., Bancroft I. Genome Res. 11:1167-1174(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[5]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[6]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 43-248. Strain: cv. Nipponbare.
[7]	"Identification of 33 rice aquaporin genes and analysis of their expression and function." Sakurai J., Ishikawa F., Yamaguchi T., Uemura M., Maeshima M. Plant Cell Physiol. 46:1568-1577(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, TISSUE SPECIFICITY, INDUCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB114830 mRNA. Translation: BAC79359.1. AJ307662 Genomic DNA. Translation: CAC39073.1. AP005006 Genomic DNA. Translation: BAD25694.1. AP005289 Genomic DNA. Translation: BAD25765.1. AP008208 Genomic DNA. Translation: BAF09546.1. CM000139 Genomic DNA. Translation: EAZ24070.1. AK064728 mRNA. No translation available.
RefSeq	NP_001047632.1. NM_001054167.1.
UniGene	Os.48060.
3D structure databases
ProteinModelPortal	Q7XA61.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os02g44080.1; LOC_Os02g44080.1; LOC_Os02g44080.
GeneID	4330207.
KEGG	osa:4330207.
Organism-specific databases
Gramene	Q7XA61.
Phylogenomic databases
eggNOG	COG0580.
HOGENOM	HOG000288286.
KO	K09873.
OMA	IGSYAPA.
ProtClustDB	CLSN2693015.
Gene expression databases
ArrayExpress	Q7XA61.
Family and domain databases
Gene3D	1.20.1080.10. 1 hit.
InterPro	IPR023271. Aquaporin-like. IPR000425. MIP. IPR022357. MIP_CS. [Graphical view]
PANTHER	PTHR19139. PTHR19139. 1 hit.
Pfam	PF00230. MIP. 1 hit. [Graphical view]
PRINTS	PR00783. MINTRINSICP.
SUPFAM	SSF81338. MIP. 1 hit.
TIGRFAMs	TIGR00861. MIP. 1 hit.
PROSITE	PS00221. MIP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TIP21_ORYSJ

Accession

Primary (citable) accession number: Q7XA61
Secondary accession number(s): Q0DYZ2, Q949B9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	October 1, 2003
Last modified:	April 3, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program