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Protein

Protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1

Gene

PIE1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant H2A.F/Z leading to transcriptional regulation of selected genes (e.g. FLC) by chromatin remodeling. Probable DNA-dependent ATPase. Not involved in the repression of FLC in gametophytes, but required for the reactivation of FLC in early embryos and for the maintenance of full activation of FLC in late embryos.4 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi561 – 568ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • covalent chromatin modification Source: UniProtKB-KW
  • defense response to bacterium Source: TAIR
  • flower development Source: UniProtKB-KW
  • response to cadmium ion Source: TAIR

Keywordsi

Molecular functionChromatin regulator, Developmental protein, DNA-binding, Helicase, Hydrolase
Biological processDifferentiation, Flowering
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 (EC:3.6.4.12)
Alternative name(s):
Independent early flowering 1 protein
Protein CHROMATIN REMODELING 131 Publication
Short name:
AtCHR13
Gene namesi
Name:PIE1
Synonyms:CHR131 Publication
Ordered Locus Names:At3g12810
ORF Names:MBK21.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G12810.
TAIRilocus:2087780. AT3G12810.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Early flowering. Loss of H2A.Z from chromatin.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004237291 – 2055Protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1Add BLAST2055

Proteomic databases

PaxDbiQ7X9V2.

PTM databases

iPTMnetiQ7X9V2.

Expressioni

Tissue specificityi

Expressed in ovules, but not in stamens.1 Publication

Inductioni

Not regulated by vernalization.1 Publication

Gene expression databases

ExpressionAtlasiQ7X9V2. baseline and differential.
GenevisibleiQ7X9V2. AT.

Interactioni

Subunit structurei

Component of the SWR1 chromatin-remodeling complex composed of at least ARP6/ESD1/SUF3, PIE1, SWC6, SWC2 and H2AZs (HTA8, HTA9, HTA11). Interacts (via c-terminus) with SWC6 and ARP6 and (via N-terminus) with H2AZs.4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi5797. 7 interactors.
IntActiQ7X9V2. 5 interactors.
STRINGi3702.AT3G12810.1.

Structurei

3D structure databases

ProteinModelPortaliQ7X9V2.
SMRiQ7X9V2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 107HSAPROSITE-ProRule annotationAdd BLAST73
Domaini548 – 713Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST166
Domaini1076 – 1229Helicase C-terminalPROSITE-ProRule annotationAdd BLAST154
Domaini1673 – 1727Myb-likePROSITE-ProRule annotationAdd BLAST55

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili78 – 147Sequence analysisAdd BLAST70
Coiled coili229 – 250Sequence analysisAdd BLAST22
Coiled coili392 – 416Sequence analysisAdd BLAST25
Coiled coili1419 – 1492Sequence analysisAdd BLAST74
Coiled coili2006 – 2029Sequence analysisAdd BLAST24

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi29 – 36Nuclear localization signal 1PROSITE-ProRule annotation8
Motifi664 – 667DEAH box4
Motifi1506 – 1513Nuclear localization signal 2PROSITE-ProRule annotation8
Motifi1570 – 1577Nuclear localization signal 3PROSITE-ProRule annotation8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi135 – 138Poly-Lys4
Compositional biasi181 – 257Glu-richAdd BLAST77
Compositional biasi503 – 509Poly-Ala7
Compositional biasi1373 – 1379Poly-Ala7
Compositional biasi1410 – 1491Glu-richAdd BLAST82
Compositional biasi1504 – 1659Lys-richPROSITE-ProRule annotationAdd BLAST156
Compositional biasi2009 – 2015Poly-Glu7

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0391. Eukaryota.
ENOG410XP0A. LUCA.
HOGENOMiHOG000083197.
InParanoidiQ7X9V2.
KOiK11320.
OMAiNDESDHT.
OrthoDBiEOG093600BW.
PhylomeDBiQ7X9V2.

Family and domain databases

InterProiView protein in InterPro
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR014012. HSA_dom.
IPR017877. Myb-like_dom.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
PfamiView protein in Pfam
PF00271. Helicase_C. 1 hit.
PF07529. HSA. 1 hit.
PF00176. SNF2_N. 1 hit.
SMARTiView protein in SMART
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00573. HSA. 1 hit.
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiView protein in PROSITE
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51204. HSA. 1 hit.
PS50090. MYB_LIKE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7X9V2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASKGGKSKP DIVMASKSGK SKPDNESRAK RQKTLEAPKE PRRPKTHWDH
60 70 80 90 100
VLEEMAWLSK DFESERKWKL AQAKKVALRA SKGMLDQASR EERKLKEEEQ
110 120 130 140 150
RLRKVALNIS KDMKKFWMKV EKLVLYKHQL VRNEKKKKAM DKQLEFLLGQ
160 170 180 190 200
TERYSTMLAE NLVEPYKQGQ NTPSKPLLTI ESKSDEERAE QIPPEINSSA
210 220 230 240 250
GLESGSPELD EDYDLKSEDE TEDDEDTIEE DEKHFTKRER QEELEALQNE
260 270 280 290 300
VDLPVEELLR RYTSGRVSRE TSPVKDENED NLTSVSRVTS PVKDENQDNL
310 320 330 340 350
ASVGQDHGED KNNLAASEET EGNPSVRRSN DSYGHLAISE THSHDLEPGM
360 370 380 390 400
TTASVKSRKE DHTYDFNDEQ EDVDFVLANG EEKDDEATLA VEEELAKADN
410 420 430 440 450
EDHVEEIALL QKESEMPIEV LLARYKEDFG GKDISEDESE SSFAVSEDSI
460 470 480 490 500
VDSDENRQQA DLDDDNVDLT ECKLDPEPCS ENVEGTFHEV AEDNDKDSSD
510 520 530 540 550
KIADAAAAAR SAQPTGFTYS TTKVRTKLPF LLKHSLREYQ HIGLDWLVTM
560 570 580 590 600
YEKKLNGILA DEMGLGKTIM TIALLAHLAC DKGIWGPHLI VVPTSVMLNW
610 620 630 640 650
ETEFLKWCPA FKILTYFGSA KERKLKRQGW MKLNSFHVCI TTYRLVIQDS
660 670 680 690 700
KMFKRKKWKY LILDEAHLIK NWKSQRWQTL LNFNSKRRIL LTGTPLQNDL
710 720 730 740 750
MELWSLMHFL MPHVFQSHQE FKDWFCNPIA GMVEGQEKIN KEVIDRLHNV
760 770 780 790 800
LRPFLLRRLK RDVEKQLPSK HEHVIFCRLS KRQRNLYEDF IASTETQATL
810 820 830 840 850
TSGSFFGMIS IIMQLRKVCN HPDLFEGRPI VSSFDMAGID VQLSSTICSL
860 870 880 890 900
LLESPFSKVD LEALGFLFTH LDFSMTSWEG DEIKAISTPS ELIKQRVNLK
910 920 930 940 950
DDLEAIPLSP KNRKNLQGTN IFEEIRKAVF EERIQESKDR AAAIAWWNSL
960 970 980 990 1000
RCQRKPTYST SLRTLLTIKG PLDDLKANCS SYMYSSILAD IVLSPIERFQ
1010 1020 1030 1040 1050
KMIELVEAFT FAIPAARVPS PTCWCSKSDS PVFLSPSYKE KVTDLLSPLL
1060 1070 1080 1090 1100
SPIRPAIVRR QVYFPDRRLI QFDCGKLQEL AMLLRKLKFG GHRALIFTQM
1110 1120 1130 1140 1150
TKMLDVLEAF INLYGYTYMR LDGSTPPEER QTLMQRFNTN PKIFLFILST
1160 1170 1180 1190 1200
RSGGVGINLV GADTVIFYDS DWNPAMDQQA QDRCHRIGQT REVHIYRLIS
1210 1220 1230 1240 1250
ESTIEENILK KANQKRVLDN LVIQNGEYNT EFFKKLDPME LFSGHKALTT
1260 1270 1280 1290 1300
KDEKETSKHC GADIPLSNAD VEAALKQAED EADYMALKRV EQEEAVDNQE
1310 1320 1330 1340 1350
FTEEPVERPE DDELVNEDDI KADEPADQGL VAAGPAKEEM SLLHSDIRDE
1360 1370 1380 1390 1400
RAVITTSSQE DDTDVLDDVK QMAAAAADAG QAISSFENQL RPIDRYAIRF
1410 1420 1430 1440 1450
LELWDPIIVE AAMENEAGFE EKEWELDHIE KYKEEMEAEI DDGEEPLVYE
1460 1470 1480 1490 1500
KWDADFATEA YRQQVEVLAQ HQLMEDLENE AREREAAEVA EMVLTQNESA
1510 1520 1530 1540 1550
HVLKPKKKKK AKKAKYKSLK KGSLAAESKH VKSVVKIEDS TDDDNEEFGY
1560 1570 1580 1590 1600
VSSSDSDMVT PLSRMHMKGK KRDLIVDTDE EKTSKKKAKK HKKSLPNSDI
1610 1620 1630 1640 1650
KYKQTSALLD ELEPSKPSDS MVVDNELKLT NRGKTVGKKF ITSMPIKRVL
1660 1670 1680 1690 1700
MIKPEKLKKG NLWSRDCVPS PDSWLPQEDA ILCAMVHEYG PNWNFVSGTL
1710 1720 1730 1740 1750
YGMTAGGAYR GRYRHPAYCC ERYRELIQRH ILSASDSAVN EKNLNTGSGK
1760 1770 1780 1790 1800
ALLKVTEENI RTLLNVAAEQ PDTEMLLQKH FSCLLSSIWR TSTRTGNDQM
1810 1820 1830 1840 1850
LSLNSPIFNR QFMGSVNHTQ DLARKPWQGM KVTSLSRKLL ESALQDSGPS
1860 1870 1880 1890 1900
QPDNTISRSR LQETQPINKL GLELTLEFPR GNDDSLNQFP PMISLSIDGS
1910 1920 1930 1940 1950
DSLNYVNEPP GEDVLKGSRV AAENRYRNAA NACIEDSFGW ASNTFPANDL
1960 1970 1980 1990 2000
KSRTGTKAQS LGKHKLSASD SAKSTKSKHR KLLAEQLEGA WVRPNDPNLK
2010 2020 2030 2040 2050
FDFTPGDREE EEEQEVDEKA NSAEIEMISC SQWYDPFFTS GLDDCSLASD

ISEIE
Length:2,055
Mass (Da):234,048
Last modified:October 1, 2003 - v1
Checksum:iED38EC3C67D7F776
GO

Sequence cautioni

The sequence BAB02425 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY279398 mRNA. Translation: AAP40633.1.
AB024033 Genomic DNA. Translation: BAB02425.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE75248.1.
RefSeqiNP_187887.3. NM_112117.5.
UniGeneiAt.39549.

Genome annotation databases

EnsemblPlantsiAT3G12810.1; AT3G12810.1; AT3G12810.
GeneIDi820463.
GrameneiAT3G12810.1; AT3G12810.1; AT3G12810.
KEGGiath:AT3G12810.

Similar proteinsi

Entry informationi

Entry nameiPIE1_ARATH
AccessioniPrimary (citable) accession number: Q7X9V2
Secondary accession number(s): Q9LTV5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: October 1, 2003
Last modified: July 5, 2017
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families