Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Wall-associated receptor kinase-like 2

Gene

WAKL2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Serine/threonine-protein kinase that may function as a signaling receptor of extracellular matrix component.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei444ATPPROSITE-ProRule annotation1
Active sitei541Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi422 – 430ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • cell surface receptor signaling pathway Source: GO_Central
  • protein autophosphorylation Source: TAIR

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Wall-associated receptor kinase-like 2 (EC:2.7.11.-)
Gene namesi
Name:WAKL2
Ordered Locus Names:At1g16130
ORF Names:T24D18.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G16130
TAIRilocus:2200562 AT1G16130

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini28 – 341ExtracellularSequence analysisAdd BLAST314
Transmembranei342 – 362HelicalSequence analysisAdd BLAST21
Topological domaini363 – 748CytoplasmicSequence analysisAdd BLAST386

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000025330628 – 748Wall-associated receptor kinase-like 2Add BLAST721

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi66N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi83N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi160N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi199N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi200N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi221N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi257N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi272 ↔ 285By similarity
Disulfide bondi308 ↔ 322By similarity
Disulfide bondi317 ↔ 331By similarity
Modified residuei489PhosphotyrosineBy similarity1
Modified residuei575PhosphothreonineBy similarity1
Modified residuei580PhosphothreonineBy similarity1
Modified residuei588PhosphotyrosineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ7X8C5

Expressioni

Tissue specificityi

Slightly expressed in the whole plant.1 Publication

Inductioni

Induced by INA.1 Publication

Gene expression databases

ExpressionAtlasiQ7X8C5 baseline and differential
GenevisibleiQ7X8C5 AT

Interactioni

Protein-protein interaction databases

BioGridi23422, 1 interactor
STRINGi3702.AT1G16130.1

Structurei

3D structure databases

ProteinModelPortaliQ7X8C5
SMRiQ7X8C5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini416 – 689Protein kinasePROSITE-ProRule annotationAdd BLAST274

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni270 – 331Atypical EGF-likeAdd BLAST62

Domaini

The EGF-like region is specific to this family of proteins and seems to consist of the C-terminal of an EGF-like domain fused to the N-terminal of another one.

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IG4X Eukaryota
COG0515 LUCA
HOGENOMiHOG000116550
InParanoidiQ7X8C5
OMAiKIRSATH
OrthoDBiEOG093602C6
PhylomeDBiQ7X8C5

Family and domain databases

InterProiView protein in InterPro
IPR018097 EGF_Ca-bd_CS
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR008271 Ser/Thr_kinase_AS
IPR013695 WAK
IPR025287 WAK_GUB
PfamiView protein in Pfam
PF13947 GUB_WAK_bind, 1 hit
PF07714 Pkinase_Tyr, 1 hit
PF08488 WAK, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01186 EGF_2, 1 hit
PS01187 EGF_CA, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7X8C5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTETHNRQC IPLAISVLSL FINGVSSARQ PPDRCNRVCG EISIPFPFGI
60 70 80 90 100
GGKDCYLNPW YEVVCNSTNS VPFLSRINRE LVNISLNGVV HIKAPVTSSG
110 120 130 140 150
CSTGTSQPLT PPPLNVAGQG SPYFLTDKNL LVAVGCKFKA VMAGITSQIT
160 170 180 190 200
SCESSCNERN SSSQEGRNKI CNGYKCCQTR IPEGQPQVIS VDIEIPQGNN
210 220 230 240 250
TTGEGGCRVA FLTSDKYSSL NVTEPEKFHG HGYAAVELGW FFDTSDSRDT
260 270 280 290 300
QPISCKNASD TTPYTSDTRC SCSYGYFSGF SYRDCYCNSP GYKGNPFLPG
310 320 330 340 350
GCVDVDECKL DIGRNQCKDQ SCVNLPGWFD CQPKKPEQLK RVIQGVLIGS
360 370 380 390 400
ALLLFAFGIF GLYKFVQKRR KLIRMRKFFR RNGGMLLKQQ LARKEGNVEM
410 420 430 440 450
SRIFSSHELE KATDNFNKNR VLGQGGQGTV YKGMLVDGRI VAVKRSKAVD
460 470 480 490 500
EDRVEEFINE VVVLAQINHR NIVKLLGCCL ETEVPVLVYE FVPNGDLCKR
510 520 530 540 550
LHDESDDYTM TWEVRLHIAI EIAGALSYLH SAASFPIYHR DIKTTNILLD
560 570 580 590 600
ERNRAKVSDF GTSRSVTIDQ THLTTQVAGT FGYVDPEYFQ SSKFTEKSDV
610 620 630 640 650
YSFGVVLVEL LTGEKPSSRV RSEENRGLAA HFVEAVKENR VLDIVDDRIK
660 670 680 690 700
DECNMDQVMS VANLARRCLN RKGKKRPNMR EVSIELEMIR SSHYDSGIHI
710 720 730 740
EDDDEEDDQA MELNFNDTWE VGATAPASMF NNASPTSDAE PLVPLRTW
Length:748
Mass (Da):83,357
Last modified:October 1, 2003 - v1
Checksum:i39B2DFDE7549E135
GO

Sequence cautioni

The sequence AAF18508 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC010924 Genomic DNA Translation: AAF18508.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE29412.1
BT008569 mRNA Translation: AAP40396.1
BT008657 mRNA Translation: AAP40469.1
AK229684 mRNA Translation: BAF01525.1
PIRiA86296
RefSeqiNP_173064.1, NM_101480.3
UniGeneiAt.41899

Genome annotation databases

EnsemblPlantsiAT1G16130.1; AT1G16130.1; AT1G16130
GeneIDi838182
GrameneiAT1G16130.1; AT1G16130.1; AT1G16130
KEGGiath:AT1G16130

Similar proteinsi

Entry informationi

Entry nameiWAKLB_ARATH
AccessioniPrimary (citable) accession number: Q7X8C5
Secondary accession number(s): Q9S9M4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: October 1, 2003
Last modified: April 25, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health