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Protein

Wall-associated receptor kinase-like 2

Gene

WAKL2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Serine/threonine-protein kinase that may function as a signaling receptor of extracellular matrix component.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei444ATPPROSITE-ProRule annotation1
Active sitei541Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi422 – 430ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • cell surface receptor signaling pathway Source: GO_Central
  • protein autophosphorylation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Wall-associated receptor kinase-like 2 (EC:2.7.11.-)
Gene namesi
Name:WAKL2
Ordered Locus Names:At1g16130
ORF Names:T24D18.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G16130.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini28 – 341ExtracellularSequence analysisAdd BLAST314
Transmembranei342 – 362HelicalSequence analysisAdd BLAST21
Topological domaini363 – 748CytoplasmicSequence analysisAdd BLAST386

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000025330628 – 748Wall-associated receptor kinase-like 2Add BLAST721

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi66N-linked (GlcNAc...)Sequence analysis1
Glycosylationi83N-linked (GlcNAc...)Sequence analysis1
Glycosylationi160N-linked (GlcNAc...)Sequence analysis1
Glycosylationi199N-linked (GlcNAc...)Sequence analysis1
Glycosylationi200N-linked (GlcNAc...)Sequence analysis1
Glycosylationi221N-linked (GlcNAc...)Sequence analysis1
Glycosylationi257N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi272 ↔ 285By similarity
Disulfide bondi308 ↔ 322By similarity
Disulfide bondi317 ↔ 331By similarity
Modified residuei575PhosphothreonineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ7X8C5.

Expressioni

Tissue specificityi

Slightly expressed in the whole plant.1 Publication

Inductioni

Induced by INA.1 Publication

Gene expression databases

GenevisibleiQ7X8C5. AT.

Interactioni

Protein-protein interaction databases

BioGridi23422. 1 interactor.
STRINGi3702.AT1G16130.1.

Structurei

3D structure databases

ProteinModelPortaliQ7X8C5.
SMRiQ7X8C5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini416 – 689Protein kinasePROSITE-ProRule annotationAdd BLAST274

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni270 – 331Atypical EGF-likeAdd BLAST62

Domaini

The EGF-like region is specific to this family of proteins and seems to consist of the C-terminal of an EGF-like domain fused to the N-terminal of another one.

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IG4X. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000116550.
InParanoidiQ7X8C5.
OMAiDRIKDEC.
OrthoDBiEOG093602C6.
PhylomeDBiQ7X8C5.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR018097. EGF_Ca-bd_CS.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR013695. WAK.
IPR025287. WAK_GUB.
[Graphical view]
PfamiPF13947. GUB_WAK_bind. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF08488. WAK. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01186. EGF_2. 1 hit.
PS01187. EGF_CA. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7X8C5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTETHNRQC IPLAISVLSL FINGVSSARQ PPDRCNRVCG EISIPFPFGI
60 70 80 90 100
GGKDCYLNPW YEVVCNSTNS VPFLSRINRE LVNISLNGVV HIKAPVTSSG
110 120 130 140 150
CSTGTSQPLT PPPLNVAGQG SPYFLTDKNL LVAVGCKFKA VMAGITSQIT
160 170 180 190 200
SCESSCNERN SSSQEGRNKI CNGYKCCQTR IPEGQPQVIS VDIEIPQGNN
210 220 230 240 250
TTGEGGCRVA FLTSDKYSSL NVTEPEKFHG HGYAAVELGW FFDTSDSRDT
260 270 280 290 300
QPISCKNASD TTPYTSDTRC SCSYGYFSGF SYRDCYCNSP GYKGNPFLPG
310 320 330 340 350
GCVDVDECKL DIGRNQCKDQ SCVNLPGWFD CQPKKPEQLK RVIQGVLIGS
360 370 380 390 400
ALLLFAFGIF GLYKFVQKRR KLIRMRKFFR RNGGMLLKQQ LARKEGNVEM
410 420 430 440 450
SRIFSSHELE KATDNFNKNR VLGQGGQGTV YKGMLVDGRI VAVKRSKAVD
460 470 480 490 500
EDRVEEFINE VVVLAQINHR NIVKLLGCCL ETEVPVLVYE FVPNGDLCKR
510 520 530 540 550
LHDESDDYTM TWEVRLHIAI EIAGALSYLH SAASFPIYHR DIKTTNILLD
560 570 580 590 600
ERNRAKVSDF GTSRSVTIDQ THLTTQVAGT FGYVDPEYFQ SSKFTEKSDV
610 620 630 640 650
YSFGVVLVEL LTGEKPSSRV RSEENRGLAA HFVEAVKENR VLDIVDDRIK
660 670 680 690 700
DECNMDQVMS VANLARRCLN RKGKKRPNMR EVSIELEMIR SSHYDSGIHI
710 720 730 740
EDDDEEDDQA MELNFNDTWE VGATAPASMF NNASPTSDAE PLVPLRTW
Length:748
Mass (Da):83,357
Last modified:October 1, 2003 - v1
Checksum:i39B2DFDE7549E135
GO

Sequence cautioni

The sequence AAF18508 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC010924 Genomic DNA. Translation: AAF18508.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29412.1.
BT008569 mRNA. Translation: AAP40396.1.
BT008657 mRNA. Translation: AAP40469.1.
AK229684 mRNA. Translation: BAF01525.1.
PIRiA86296.
RefSeqiNP_173064.1. NM_101480.3.
UniGeneiAt.41899.

Genome annotation databases

EnsemblPlantsiAT1G16130.1; AT1G16130.1; AT1G16130.
GeneIDi838182.
GrameneiAT1G16130.1; AT1G16130.1; AT1G16130.
KEGGiath:AT1G16130.

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC010924 Genomic DNA. Translation: AAF18508.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29412.1.
BT008569 mRNA. Translation: AAP40396.1.
BT008657 mRNA. Translation: AAP40469.1.
AK229684 mRNA. Translation: BAF01525.1.
PIRiA86296.
RefSeqiNP_173064.1. NM_101480.3.
UniGeneiAt.41899.

3D structure databases

ProteinModelPortaliQ7X8C5.
SMRiQ7X8C5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi23422. 1 interactor.
STRINGi3702.AT1G16130.1.

Proteomic databases

PaxDbiQ7X8C5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G16130.1; AT1G16130.1; AT1G16130.
GeneIDi838182.
GrameneiAT1G16130.1; AT1G16130.1; AT1G16130.
KEGGiath:AT1G16130.

Organism-specific databases

TAIRiAT1G16130.

Phylogenomic databases

eggNOGiENOG410IG4X. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000116550.
InParanoidiQ7X8C5.
OMAiDRIKDEC.
OrthoDBiEOG093602C6.
PhylomeDBiQ7X8C5.

Miscellaneous databases

PROiQ7X8C5.

Gene expression databases

GenevisibleiQ7X8C5. AT.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR018097. EGF_Ca-bd_CS.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR013695. WAK.
IPR025287. WAK_GUB.
[Graphical view]
PfamiPF13947. GUB_WAK_bind. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF08488. WAK. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01186. EGF_2. 1 hit.
PS01187. EGF_CA. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWAKLB_ARATH
AccessioniPrimary (citable) accession number: Q7X8C5
Secondary accession number(s): Q9S9M4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: October 1, 2003
Last modified: November 30, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.