Reviewed, UniProtKB/Swiss-Prot Q7X7E9 (DNLI4_ORYSJ)

Names and origin

Protein names

Recommended name:
    Putative DNA ligase 4
    EC=6.5.1.1
Alternative name(s):
    DNA ligase IV
    Polydeoxyribonucleotide synthase [ATP] 4

Gene names

Name:	LIG4
Ordered Locus Names:	LOC_Os04g51700
ORF Names:	OSJNBa0035M09.8

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	1322 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Subcellular location	NucleusBy similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family. Contains 2 BRCT domains.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair DNA replication
Cellular component	Nucleus
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: InterPro DNA repair Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1322	1322	Putative DNA ligase 4		PRO_0000247468
Regions
	Domain	686 – 768	83	BRCT 1
	Domain	825 – 935	111	BRCT 2
Sites
	Active site	267	1	N6-AMP-lysine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7X7E9-1 [UniParc]. Last modified July 5, 2004. Version 2. Checksum: 110BF7B94DB62A67	FASTA	1,322	149,078
	10 20 30 40 50 60 MAATVRFGLL VAMFQAMSRD RTAAKKRARL RALLDRAYGP GGRDDYFSAL RLVLPGLDRE 70 80 90 100 110 120 RGSYGLKEAA LASVLVDALG IAKDSPDAVR LTNWRRGGGF RNAGNFALVA AEVLQRRQGM 130 140 150 160 170 180 TSGGLTIKEV NDALDRLAAT ENSVCLESEK LLLILDRSEK ASILSSLIKK TNALEMKWLL 190 200 210 220 230 240 MIILKDLKLG ISEKSVFHEF HPDAEDLFNV TCDLRLVCEK LNDRSQRHKR QDIEVGKAVR 250 260 270 280 290 300 PQLSMRVNNA SSAWKKLHGK QVVAECKFDG DRIQIHKNGE EIHFFSRSFL DHSEYAPGMS 310 320 330 340 350 360 KVIIENILVD RCILDGEMLV WDTVLNRFAE FGSNQEIAKA AKEGLETDRQ LCCILEWIAS 370 380 390 400 410 420 YVLALCVNIL SCQDFFFSNK RESYVAFDIL YAGDTSVIHQ SLTERHEILQ KVVRPLKGHL 430 440 450 460 470 480 EILVPTGGLN IHRPPDEPCW SILAHSLDDV EKFFKDTVDN RFFPTSPNTD ELSNLSISLM 490 500 510 520 530 540 IQFRFSDYSI CYVLREEGII LKDLESKWEP GDRSGKWLKL KPDYIHAGAD LDVIIIGGYY 550 560 570 580 590 600 GSGRRGGEVA QFLVGLAVPS DDNSYPKRFL SFCRVGTGLS DEELDALVTK LKPHFRKNEY 610 620 630 640 650 660 PKKPPRFYEV TNHSKERPDV WIESPDKQSV RFRKHLIYCI TKCRSVIISI TSDIRTIKSE 670 680 690 700 710 720 VFAAPYSLRF PRIQRLRYDK PWHECLDVQA FVDIVHSSNG TTHRAADDDN DLKNVKNGGS 730 740 750 760 770 780 FSMNLNDSVT HCIAAEKKAT RQGRIIHYSW ILDCCKEKRL LHLQPKFDPC LQPMHKFPEE 790 800 810 820 830 840 IDSYADYFYW DIDISDLKQI FSNMDRAVVD SNMVHHYKKK YCADERFCFF QGCCVYFYHA 850 860 870 880 890 900 PLVNADYNVI SDLALKRVKQ DLTMHGGQVC SILAPATHLI IVSVLQAYNF DMLYKSLPPA 910 920 930 940 950 960 ERRYLHDKRL QVVSNKWLED SVEKQTRLPE TTYSLKPDTL EEIEIERSEE TVQPCNDKLE 970 980 990 1000 1010 1020 ENEKADTSHV KHAPRKRGRP SSSASRTAKP APRPVRRTRA RRGNQHAKID DVEPEESDHG 1030 1040 1050 1060 1070 1080 ETGLDDQIPD TDNISKMEVD SFDKDQVSAR PVRRTRARRG KQHAKIDYGQ SEESDPGETG 1090 1100 1110 1120 1130 1140 QDDQRLDADY ISKMEEDSSD RDQGAHPTAP RVVRRSRAQR GKWLAKIDRE TGPGETGQDD 1150 1160 1170 1180 1190 1200 KKLNADSISK MEEHAHDKDQ EPPPGAQLIT LDEQEPKGIK SSTTETPSSP KHERNQTVLR 1210 1220 1230 1240 1250 1260 RDTAETTSSA TCEKMEQMVD PLHAMLLDMI PSLGQMKTDV GNRVAEAKAE TNPPWVGSST 1270 1280 1290 1300 1310 1320 SSYVAPVPQA SASSASSSGV PAPHAGSSTQ STGVPAPDPT AGAPKKKKVS YKDVAGALLK DW

References

[1]

"Sequence and analysis of rice chromosome 4." Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y., Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q., Zhang L. , Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T., Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R., Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G., Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C., Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J., Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J., Li J., Hong G., Xue Y., Han B. Nature 420:316-320(2002) [PubMed: 12447439] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL662968 Genomic DNA. Translation: CAD41778.2.
UniGene	Os.53597
3D structure databases
ModBase	Search...
Organism-specific databases
Gramene	Q7X7E9.
Family and domain databases
InterPro	IPR001357. BRCT. IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 2 hits. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS50172. BRCT. 2 hits. PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI4_ORYSJ

Accession

Primary (citable) accession number: Q7X7E9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 5, 2004
Last modified:	November 25, 2008
This is version 31 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)