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Q7X5K6 (RNPA_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease P protein component

Short name=RNase P protein
Short name=RNaseP protein
EC=3.1.26.5
Alternative name(s):
Protein C5
Gene names
Name:rnpA
Ordered Locus Names:TTHA0445
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length163 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme. HAMAP-Rule MF_00227

Catalytic activity

Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor. HAMAP-Rule MF_00227

Subunit structure

Consists of a catalytic RNA component (M1 or rnpB) and a protein subunit.

Miscellaneous

The open reading frame (ORF) for this protein entirely encompasses the ORF for the 50S ribosomal protein L34 (rpmH). The two start codons are separated by four nucleotides.

Sequence similarities

Belongs to the RnpA family.

Sequence caution

The sequence BAD70268.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processtRNA processing
   LigandRNA-binding
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionribonuclease P activity

Inferred from electronic annotation. Source: UniProtKB-EC

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 163163Ribonuclease P protein component HAMAP-Rule MF_00227
PRO_0000198559

Experimental info

Mutagenesis1 – 5050Missing: No effect on activity of the reconstituted enzyme.

Sequences

Sequence LengthMass (Da)Tools
Q7X5K6 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 7B30582D2C2D3421

FASTA16317,851
        10         20         30         40         50         60 
MDEKDVATQP QETGQNPRLS GQDEDPGRPE GAEAPPSEGA LAPHARRSEA VGPKPPAPGG 

        70         80         90        100        110        120 
KLLSLKGDRA FQRLRKGRAG RGRYVSVKWL PAAELRVGIV VSKKVGKAVV RNKVKRRLRE 

       130        140        150        160 
ILRRLHLPQA HLLVVASPEA READFAELFR DVVRALRKSG LVQ 

« Hide

References

« Hide 'large scale' references
[1]"An unusual mechanism of bacterial gene expression revealed for the RNase P protein of Thermus strains."
Feltens R., Gossringer M., Willkomm D.K., Urlaub H., Hartmann R.K.
Proc. Natl. Acad. Sci. U.S.A. 100:5724-5729(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-61; 89-96; 125-154 AND 158-163, CHARACTERIZATION, MUTAGENESIS.
[2]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY256342 Genomic DNA. Translation: AAO88977.1.
AP008226 Genomic DNA. Translation: BAD70268.1. Different initiation.
RefSeqYP_143711.1. NC_006461.1.

3D structure databases

ProteinModelPortalQ7X5K6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING300852.TTHA0445.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD70268; BAD70268; BAD70268.
GeneID3170122.
KEGGttj:TTHA0445.
PATRIC23955859. VBITheThe93045_0444.

Phylogenomic databases

eggNOGbactNOG50607.
HOGENOMHOG000266301.
KOK03536.
OMAHLPPCEM.
OrthoDBEOG6C01C6.
ProtClustDBCLSK738259.

Enzyme and pathway databases

BioCycTTHE300852:GH8R-466-MONOMER.

Family and domain databases

Gene3D3.30.230.10. 1 hit.
HAMAPMF_00227. RNase_P.
InterProIPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR000100. RNase_P.
IPR020539. RNase_P_CS.
[Graphical view]
PfamPF00825. Ribonuclease_P. 1 hit.
[Graphical view]
ProDomPD003629. Ribonuclease_P. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF54211. SSF54211. 1 hit.
TIGRFAMsTIGR00188. rnpA. 1 hit.
PROSITEPS00648. RIBONUCLEASE_P. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRNPA_THET8
AccessionPrimary (citable) accession number: Q7X5K6
Secondary accession number(s): Q5SL48
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: October 1, 2003
Last modified: April 16, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families