Q7X5K6 (RNPA_THET8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease P protein component
Short name=RNase P protein
Short name=RNaseP protein
EC=3.1.26.5

Alternative name(s):
Protein C5

Gene names

Name:	rnpA
Ordered Locus Names:	TTHA0445

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus › Thermus thermophilus

Protein attributes

Sequence length	163 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme. HAMAP-Rule MF_00227
Catalytic activity	Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor. HAMAP-Rule MF_00227
Subunit structure	Consists of a catalytic RNA component (M1 or RnpB) and a protein subunit.
Miscellaneous	The open reading frame (ORF) for this protein entirely encompasses the ORF for the 50S ribosomal protein L34 (rpmH). The two start codons are separated by four nucleotides. HAMAP-Rule MF_00227
Sequence similarities	Belongs to the RnpA family.
Sequence caution	The sequence BAD70268.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	RNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC tRNA 5'-leader removal Inferred from electronic annotation. Source: HAMAP
Molecular_function	ribonuclease P activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 163	163	Ribonuclease P protein component HAMAP-Rule MF_00227		PRO_0000198559
Experimental info
	Mutagenesis	1 – 50	50	Missing: No effect on activity of the reconstituted enzyme.

Sequences

	Sequence		Length	Mass (Da)
	Q7X5K6 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 7B30582D2C2D3421	FASTA	163	17,851
	10 20 30 40 50 60 MDEKDVATQP QETGQNPRLS GQDEDPGRPE GAEAPPSEGA LAPHARRSEA VGPKPPAPGG 70 80 90 100 110 120 KLLSLKGDRA FQRLRKGRAG RGRYVSVKWL PAAELRVGIV VSKKVGKAVV RNKVKRRLRE 130 140 150 160 ILRRLHLPQA HLLVVASPEA READFAELFR DVVRALRKSG LVQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An unusual mechanism of bacterial gene expression revealed for the RNase P protein of Thermus strains." Feltens R., Gossringer M., Willkomm D.K., Urlaub H., Hartmann R.K. Proc. Natl. Acad. Sci. U.S.A. 100:5724-5729(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-61; 89-96; 125-154 AND 158-163, CHARACTERIZATION, MUTAGENESIS.
[2]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB8 / ATCC 27634 / DSM 579.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY256342 Genomic DNA. Translation: AAO88977.1. AP008226 Genomic DNA. Translation: BAD70268.1. Different initiation.
RefSeq	YP_143711.1. NC_006461.1.
3D structure databases
ProteinModelPortal	Q7X5K6.
ModBase	Search...
Protein-protein interaction databases
STRING	300852.TTHA0445.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAD70268; BAD70268; BAD70268.
GeneID	3170122.
KEGG	ttj:TTHA0445.
PATRIC	23955859. VBITheThe93045_0444.
Phylogenomic databases
eggNOG	bactNOG50607.
HOGENOM	HOG000266301.
KO	K03536.
OMA	AVEASYW.
ProtClustDB	CLSK738259.
Family and domain databases
Gene3D	3.30.230.10. 1 hit.
HAMAP	MF_00227. RNase_P.
InterPro	IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR000100. RNase_P. IPR020539. RNase_P_CS. [Graphical view]
Pfam	PF00825. Ribonuclease_P. 1 hit. [Graphical view]
ProDom	PD003629. Ribonuclease_P. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00188. rnpA. 1 hit.
PROSITE	PS00648. RIBONUCLEASE_P. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPA_THET8

Accession

Primary (citable) accession number: Q7X5K6
Secondary accession number(s): Q5SL48

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2004
Last sequence update:	October 1, 2003
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program