Endo-beta-1,4-glucanase - Bacillus licheniformis

Preferred order of sections

Names and origin

Protein names

Submitted name:
Endo-beta-1,4-glucanase EMBL AAP44491.1
EC=3.2.1.4 EMBL AAP44491.1

Gene names

Name:

cel12A EMBL AAP44491.1

Organism

Bacillus licheniformis EMBL AAP44491.1

Taxonomic identifier

1402 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Molecular function	Glycosidase EMBL AAP44491.1 Hydrolase
Technical term	3D-structure PDB 2JEN PDB 2JEM
Gene Ontology (GO)
Biological process	polysaccharide catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Region

51 – 53

3

Glucose binding PDB 2JEN

Sites

Binding site

97

1

Glucose PDB 2JEN

Binding site

137

1

Glucose PDB 2JEN

Binding site

157

1

Glucose PDB 2JEN

Binding site

166

1

Glucose; via carbonyl oxygen PDB 2JEN

Binding site

197

1

Glucose PDB 2JEN

Binding site

243

1

Glucose PDB 2JEN

Sequences

Sequence

Length

Mass (Da)

Tools

Q7X4S4 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 444CA54F697D95C3
Show »

FASTA

261

29,068

        10         20         30         40         50         60 
MKNNHLLKSI LLWGAVCIIV LAGPLSAFAA SSSNPSDKLY FKNKKYYIFN NVWGADQVSG 

        70         80         90        100        110        120 
WWQTIYHNSD SDMGWVWNWP SNTSTVKAYP SIVSGWHWTE GYTAGSGFPT RLSDQKNINT 

       130        140        150        160        170        180 
KVSYSISANG TYNAAYDIWL HNTNKASWDS APTDEIMIWL NNTNAGPAGS YVETVSIGGH 

       190        200        210        220        230        240 
SWKVYKGYID AGGGKGWNVF SFIRTANTQS ANLNIRDFTN YLADSKQWLS KTKYVSSVEF 

       250        260 
GTEVFGGTGQ INISNWDVTV R

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References

[1]

"Molecular cloning of novel cellulase genes cel9A and cel12A from Bacillus licheniformis GXN151 and synergism of their encoded polypeptides."
Liu Y., Zhang J., Liu Q., Zhang C., Ma Q.
Curr. Microbiol. 49:234-238(2004) [PubMed: 15386110] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: GXN151 EMBL AAP44491.1.

[2]

"Characterization and three-dimensional structures of two distinct bacterial xyloglucanases from families GH5 and GH12."
Gloster T.M., Ibatullin F.M., Macauley K., Eklof J.M., Roberts S., Turkenburg J.P., Bjornvad M.E., Jorgensen P.L., Danielsen S., Johansen K.S., Borchert T.V., Wilson K.S., Brumer H., Davies G.J.
J. Biol. Chem. 282:19177-19189(2007) [PubMed: 17376777] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) IN COMPLEX WITH GLUCOSE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY291066 Genomic DNA. Translation: AAP44491.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2JEM	X-ray	1.78	A/B	1-261	[»]
2JEN	X-ray	1.40	A	1-261	[»]

ProteinModelPortal

Q7X4S4.

SMR

Q7X4S4. Positions 29-261.

ModBase

Search...

Protein family/group databases

CAZy

GH12. Glycoside Hydrolase Family 12.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR008985. ConA-like_lec_gl.
IPR013319. Glyco_hydro_11/12_cat.
IPR002594. Glyco_hydro_12.
[Graphical view]

Gene3D

G3DSA:2.60.120.180. Glyco_hydro_11/12_cat. 1 hit.

Pfam

PF01670. Glyco_hydro_12. 1 hit.
[Graphical view]

SUPFAM

SSF49899. ConA_like_lec_gl. 1 hit.

ProtoNet

Search...

Entry information

Entry name

Q7X4S4_BACLI

Accession

Primary (citable) accession number: Q7X4S4

Entry history

Integrated into UniProtKB/TrEMBL:	October 1, 2003
Last sequence update:	October 1, 2003
Last modified:	July 27, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)