Reviewed, UniProtKB/Swiss-Prot Q7X3P1 (SPEB_PROMI)

Names and origin

Protein names

Recommended name:
    Agmatinase
    EC=3.5.3.11
Alternative name(s):
    Agmatine ureohydrolase
      Short name=AUH

Gene names

Name:

speB

Organism

Proteus mirabilis

Taxonomic identifier

584 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Proteus

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of putrescine from agmatine By similarity.
Catalytic activity	Agmatine + H2O = putrescine + urea. HAMAP MF_01418
Cofactor	Manganese By similarity.
Pathway	Amine and polyamine biosynthesis; putrescine biosynthesis via agmatine pathway; putrescine from agmatine: step 1/1. HAMAP MF_01418
Disruption phenotype	Cells show a delay in differentiation to swarmer cells and are unable to migrate effectively on agar surfaces. Putrescine restores normal cell differentiation and migration ability. Ref.1
Sequence similarities	Belongs to the arginase family. Agmatinase subfamily.

Ontologies

Keywords
Biological process	Polyamine biosynthesis Putrescine biosynthesis Spermidine biosynthesis
Ligand	Manganese Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	putrescine biosynthetic process Inferred from electronic annotation. Source: HAMAP spermidine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	agmatinase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Agmatinase HAMAP MF_01418		PRO_0000173738
Sites
	Metal binding	128	1	Manganese By similarity
	Metal binding	151	1	Manganese By similarity
	Metal binding	153	1	Manganese By similarity
	Metal binding	155	1	Manganese By similarity
	Metal binding	232	1	Manganese By similarity
	Metal binding	234	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7X3P1-1 [UniParc]. Last modified March 29, 2004. Version 2. Checksum: BFAD491A8881C77B	FASTA	306	33,473
	10 20 30 40 50 60 MKNCTLGNET DNSLISNAFG FLRFPLNFQP YSSDADWVIT GVPFDMATSG RAGTRHGPGA 70 80 90 100 110 120 IRQISTNLAW EGHRWPWHFD MRERLKVVDC GDLVFNFGDA QDMSDKLQAH TEKLLAAGKR 130 140 150 160 170 180 CLTFGGDHFV TLPLLRAHAK HFGKMALVHF DAHTDTYANG SKFDHGTMFY HAPNEGLIDP 190 200 210 220 230 240 QHSVQIGIRT EHDTNNGFTV LDAAQVNDRG VDDLVAQIKE IVGSLPVYLT FDIDCLDPAF 250 260 270 280 290 300 APGTGTPVVG GLTTDKALKM LRALQPLNIV GMDLVEVSPA YDQSDITALA GATIALDMLY LQAAKK

References

[1]	"Evidence that putrescine acts as an extracellular signal required for swarming in Proteus mirabilis." Sturgill G., Rather P.N. Mol. Microbiol. 51:437-446(2004) [PubMed: 14756784] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY298901 Genomic DNA. Translation: AAP55488.1. Different initiation.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.5.3.11. 639.
Family and domain databases
HAMAP	MF_01418. [Tree]
InterPro	IPR005925. Agmatinase. IPR006035. Ureohydrolase. [Graphical view]
Gene3D	G3DSA:3.40.800.10. Ureohydrolase. 1 hit.
PANTHER	PTHR11358. Ureohydrolase. 1 hit.
Pfam	PF00491. Arginase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01230. agmatinase. 1 hit.
PROSITE	PS01053. ARGINASE_1. 1 hit. PS51409. ARGINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPEB_PROMI

Accession

Primary (citable) accession number: Q7X3P1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2004
Last sequence update:	March 29, 2004
Last modified:	July 28, 2009
This is version 28 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)